| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 2,823,871 | A→G | 82.0% | intergenic (+616/+342) | nrfG → / ← pheU | heme lyase (NrfEFG) for insertion of heme into c552, subunit NrfG/tRNA‑Phe |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 2,823,871 | 0 | A | G | 82.0% | 154.8 / 25.1 | 72 | intergenic (+616/+342) | nrfG/pheU | heme lyase (NrfEFG) for insertion of heme into c552, subunit NrfG/tRNA‑Phe |
| Reads supporting (aligned to +/- strand): ref base A (3/10); new base G (28/31); total (31/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.32e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.46e-01 | |||||||||||
CCAAAGAAAAATGTTCAGAAAATAATCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGATTATTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAATCTCATCAG > Exported/2823804‑2823937 | ccAAAGAAAAATGTTCAGAAAATAATCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGAttatta < 1:155501/71‑1 (MQ=255) aGAAAAATGTTCAGAAAATAATCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGATTGTTAGTCt < 1:1199788/71‑1 (MQ=255) aaaaTGTTCAGAAAATAATCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGatta > 1:4950190/1‑61 (MQ=255) aaaaTGTTCAGAAAATAATCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGATTGTTAGTCTTTg < 1:137084/71‑1 (MQ=255) aaaTGTTCAGAAAATAATCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGATTGTTAGTCTTTg > 1:4410463/1‑70 (MQ=255) ttCAGAAAATAATCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGATTATTAGTCTTTGc < 1:3937482/66‑1 (MQ=255) tCAGAAAATAATCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGATTGTTAGTCTTTGCATGAg > 1:5300592/1‑70 (MQ=255) cAGAAAATAATCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGATTGTTAGTCTTTGCATGAGc > 1:4034402/1‑70 (MQ=255) aaaaTAATCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAGc > 1:3855404/1‑71 (MQ=255) aaaTAATCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGATTATTAGTCTTTGCATGAGCAAGCg < 1:1732461/71‑1 (MQ=255) taatCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGATTGTTAGTCTTTGCATGa < 1:950350/61‑1 (MQ=255) taatCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGATTGTTAGTCTTTGCATGa < 1:5010144/61‑1 (MQ=255) taatCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAGCGAAc > 1:1464007/1‑71 (MQ=255) atCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAGCGAACt > 1:2241350/1‑70 (MQ=255) atCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAGCGAACt < 1:468637/70‑1 (MQ=255) atCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAGCGAACt < 1:1009274/70‑1 (MQ=255) atCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAGCGAACTg < 1:778565/71‑1 (MQ=255) cATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAGCGAACTGATa > 1:4759447/1‑71 (MQ=255) cATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAGCGAACTGATa > 1:1325757/1‑71 (MQ=255) aTGGAAAATTGTCCGGAGCACTTACTATTTTAATGGAttgtta > 1:3009616/1‑43 (MQ=255) tGGAAAATTGTCCGGAGCACTTACTATTTTAATGGATTGTTAGTCTTTGCATGAGCa < 1:2467209/57‑1 (MQ=255) ggAAAATTGTCCGGAGCACTTACTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAGCGaa > 1:4207375/1‑62 (MQ=255) ggAAAATTGTCCGGAGCACTTACTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAGCGaa > 1:418279/1‑62 (MQ=255) aaTTGTCCGGAGCACTTACTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAGCGAACTGATACATTtct > 1:3479534/1‑71 (MQ=255) aaTTGTCCGGAGCACTTACTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAGCGAACTGATACATTtct > 1:1923813/1‑71 (MQ=255) aTTGTCCGGAGCACTTACTATTTTAATGGATTGTTAGTCTTTGCATg > 1:2479940/1‑47 (MQ=255) aTTGTCCGGAGCACTTACTATTTTAATGGATTGTTAGTCTTTGCATg > 1:1228875/1‑47 (MQ=255) tCCGGAGCACTTACTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCttt < 1:2875738/70‑1 (MQ=255) tCCGGAGCACTTACTATTTTAATGGATTATTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTg < 1:3673055/71‑1 (MQ=255) cGGAGCACTTACTATTTTAATGGATTGTTAGTCTTTGCATg < 1:4655763/41‑1 (MQ=255) cGGAGCACTTACTATTTTAATGGATTGTTAGTCTTTGCATg < 1:1752600/41‑1 (MQ=255) cGGAGCACTTACTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGt > 1:4044053/1‑70 (MQ=255) ggAGCACTTACTATTTTAATGGATTGTTAGTCTTTGc < 1:1708501/37‑1 (MQ=255) aGCACTTACTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAGCGAACTGATACAt < 1:955252/57‑1 (MQ=255) gCACTTACTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCa > 1:1603865/1‑71 (MQ=255) gCACTTACTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCa > 1:1321533/1‑71 (MQ=255) gCACTTACTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCa > 1:4115694/1‑71 (MQ=255) cACTTACTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTtctc < 1:167283/69‑1 (MQ=255) cACTTACTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAGCGAACTGACACATTTCTCTTTGTtctc < 1:1267883/69‑1 (MQ=255) aCTTACTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAGCGaa > 1:4468569/1‑45 (MQ=255) aCTTACTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAGCGaa > 1:1540343/1‑45 (MQ=255) cTTACTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCAt > 1:4262048/1‑69 (MQ=255) cTTACTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCAt > 1:4355258/1‑69 (MQ=255) cTTACTATTTTAATGGATTATTAGTCTTTGCATGAGCAAGCg < 1:5071217/42‑1 (MQ=255) ttACTATTTTAATTGATTGTTAGTCTTTGCATGAGCAAGCGAACTGa < 1:4067033/47‑1 (MQ=255) ttACTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGtt < 1:4150832/63‑1 (MQ=255) ttACTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGtt < 1:5203381/63‑1 (MQ=255) aCTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAGCGAACTg > 1:928502/1‑44 (MQ=255) aCTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAGCGAACTGATACATTTctct > 1:626071/1‑56 (MQ=255) aCTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCa < 1:633104/69‑1 (MQ=255) aCTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAg > 1:2837933/1‑70 (MQ=255) ctgttttAATGGATTATTAATCTTTGCATATTCAAATGGACTGATAAACCTTTCTCTGTTTACATCCAGaa < 1:725613/68‑1 (MQ=255) ctgttttAATGGATTATTAATCTTTGCATATTCAAATGGACTGATAAACCTTTCTCTGTTTACATCCAGaa < 1:1852988/68‑1 (MQ=255) cTATTTTAATGGATTGTTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGa > 1:4186092/1‑70 (MQ=255) cTATTTTAATGGATTATTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGa > 1:3934662/1‑70 (MQ=255) cTATTTTAATGGATTATTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGa > 1:1301788/1‑70 (MQ=255) ttttAATGGATTATTAGTCTTTGCATGAGCAAGCg < 1:3152668/35‑1 (MQ=255) ttttAATGGATTATTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAAt > 1:1164936/1‑71 (MQ=255) ttttAATGGATTATTAATCTTTGCATATTCAAATGGACTGATAAACCTTTCTCTGTTTACATCCAGaaa < 1:2004004/69‑1 (MQ=255) ttAATGGATTGTTAGTCTTTGCATGAGCAAGCGAACTGATACa > 1:5000199/1‑43 (MQ=255) ttAATGGATTGTTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTAATTCAGAAAAta > 1:2056989/1‑69 (MQ=255) tAATGGATTGTTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAAtct < 1:5614916/70‑1 (MQ=255) tAATGGATTGTTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAAtct < 1:2148017/70‑1 (MQ=255) aaTGGATTGTTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAAtctc < 1:5124004/70‑1 (MQ=255) aTGGATTGTTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCAtt < 1:3294995/58‑1 (MQ=255) aTGGATTGTTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCAtt < 1:4147399/58‑1 (MQ=255) aTGGATTGTTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCAtt < 1:4392639/58‑1 (MQ=255) aTGGATTGTTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAAtctc < 1:1486434/69‑1 (MQ=255) aTGGATTGTTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAATCtca < 1:4002675/70‑1 (MQ=255) gATTGTTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAATCTCATCAg < 1:2246936/71‑1 (MQ=255) gATTGTTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAATCTCATCAg < 1:695184/71‑1 (MQ=255) gATTGTTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAATCTCATCAg < 1:2968525/71‑1 (MQ=255) gATTGTTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAATCTCATCAg < 1:3915967/71‑1 (MQ=255) gATTATTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAATCtcatca < 1:3081504/70‑1 (MQ=255) gATTATTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAATCtcatca < 1:3092828/70‑1 (MQ=255) | CCAAAGAAAAATGTTCAGAAAATAATCCATGGAAAATTGTCCGGAGCACTTACTATTTTAATGGATTATTAGTCTTTGCATGAGCAAGCGAACTGATACATTTCTCTTTGTTCTCATTCAGAAAATCTCATCAG > Exported/2823804‑2823937 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |