Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,872,305 A→G 7.6% S186G (AGT→GGT)  ulaF → L‑ribulose 5‑phosphate 4‑epimerase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,872,3050AG 7.6% 259.7 / 8.6 92S186G (AGT→GGT) ulaFL‑ribulose 5‑phosphate 4‑epimerase
Reads supporting (aligned to +/- strand):  ref base A (33/52);  new base G (5/2);  total (38/54)
Fisher's exact test for biased strand distribution p-value = 1.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01

Only 100 of 104 total aligned reads displayed.

TCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGA  >  Exported/2872236‑2872375
                                                                     |                                                                      
tCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGgaagaa                                                                        <  1:4547312/70‑1 (MQ=255)
  cccACGGCCCGTTTGCATGGGGCAAAAATGCCGAAGATGCGGTGCATAACGCCATCGTGCTGGAAGAGGtc                                                                     >  1:2887968/3‑70 (MQ=255)
  cccACGGCCCGTTTGCATGGGGCAAAAATGCCGAAGATGCGGTGCATAACGCCATCGTGCTGGAAGAGGtc                                                                     >  1:4479915/3‑70 (MQ=255)
  cccACGGCCCGTTTGCATGGGGCAAAAATGCCGAAGATGCGGTGCATAACGCCATCGTGCTGGAAGAGGt                                                                      >  1:1172157/3‑70 (MQ=255)
  aGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGt                                                                      <  1:4557762/70‑1 (MQ=255)
  aGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTg                                                                     <  1:3622580/71‑1 (MQ=255)
  aGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTg                                                                     <  1:4289954/71‑1 (MQ=255)
   ccACGGCCCGTTTGCATGGGGCAAAAATGCCGAAGATGCGGTGCATAACGCCATCGTGCTGGAAGAGGtc                                                                     >  1:3668662/2‑69 (MQ=255)
    cACGGCCCGTTTGCATGGGGCAAAAATGCCGAAGATGCGGTGCATAACGCCATCGTGCTGGAAGAGGTCGc                                                                   <  1:1034563/71‑1 (MQ=255)
     aCGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGt                                                                      >  1:3283436/1‑67 (MQ=255)
     aCGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGt                                                                      >  1:5679899/1‑67 (MQ=255)
     aCGGCCCGTTTGCATGGGGCAAAAATGCCGAAGATGCGGTGCATAACGCCATCGTGCTGGAAGAGGTCGct                                                                  >  1:648511/1‑70 (MQ=255)
     aCGGCCCGTTTGCATGGGGCAAAAATGCCGAAGATGCGGTGCATAACGCCATCGTGCTGGAAGAGGTCGct                                                                  <  1:744477/71‑2 (MQ=255)
      cGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCg                                                                  >  1:3421720/1‑70 (MQ=255)
      cGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGa                                                                 <  1:394521/71‑1 (MQ=255)
      cGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGa                                                                 <  1:5533142/71‑1 (MQ=255)
       gggCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGa                                                                 >  1:80711/1‑70 (MQ=255)
         gCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGaaaa                                                              <  1:4340449/71‑1 (MQ=255)
         gCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGaaaa                                                              >  1:209393/1‑71 (MQ=255)
          ccGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGaaaa                                                              <  1:4346794/70‑1 (MQ=255)
            gTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATgg                                                           >  1:1590732/1‑71 (MQ=255)
                gCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTg                                                       <  1:4904463/71‑1 (MQ=255)
                gCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTg                                                       <  1:4897729/71‑1 (MQ=255)
                gCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTg                                                       <  1:4288885/71‑1 (MQ=255)
                   tGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGAt                                                    >  1:3644140/1‑71 (MQ=255)
                   tGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGAt                                                    >  1:5132382/1‑71 (MQ=255)
                   tGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGAt                                                    >  1:351066/1‑71 (MQ=255)
                    gggggAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGt                                                                      <  1:1929678/52‑1 (MQ=255)
                     ggggAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGAt                                                    <  1:1433365/69‑1 (MQ=255)
                         aaaGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGt                                                                      <  1:1849441/47‑1 (MQ=255)
                          aaGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCcgc                                             >  1:490196/1‑71 (MQ=255)
                           aGATTCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGt                                                                      <  1:4170256/45‑1 (MQ=255)
                           aGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGt                                                                      <  1:429674/45‑1 (MQ=255)
                           aGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGt                                                                      <  1:2794660/45‑1 (MQ=255)
                           aGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGt                                                                      <  1:4422703/45‑1 (MQ=255)
                           aGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTgg                                                                    >  1:3316616/1‑47 (MQ=255)
                           aGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTgg                                                                    >  1:4602987/1‑47 (MQ=255)
                           aGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTgg                                                                    >  1:148463/1‑47 (MQ=255)
                           aGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTgg                                                                    >  1:2661147/1‑47 (MQ=255)
                           aGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGaa                                                                <  1:1144192/51‑1 (MQ=255)
                           aGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGaa                                                                <  1:4427771/51‑1 (MQ=255)
                           aGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGaa                                                                <  1:4716890/51‑1 (MQ=255)
                           aGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGaa                                                                <  1:1545982/51‑1 (MQ=255)
                             aTGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCgg                                           >  1:1123374/1‑70 (MQ=255)
                             aTGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCgg                                           >  1:4235227/1‑70 (MQ=255)
                               gCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCAt                                        >  1:2695070/1‑71 (MQ=255)
                                cTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCAtt                                       <  1:2629783/71‑1 (MQ=255)
                                cTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCAt                                        <  1:796782/70‑1 (MQ=255)
                                 tCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCAtt                                       >  1:2634633/1‑70 (MQ=255)
                                 tCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCAt                                        <  1:66643/69‑1 (MQ=255)
                                   aCGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTaa                                     <  1:1990982/70‑1 (MQ=255)
                                   aCGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAAc                                    >  1:3027528/1‑71 (MQ=255)
                                   aCGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAAc                                    >  1:803648/1‑71 (MQ=255)
                                    cGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTg                                                       <  1:1080159/51‑1 (MQ=255)
                                    cGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTg                                                       <  1:278121/51‑1 (MQ=255)
                                    cGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTg                                                       <  1:1450193/51‑1 (MQ=255)
                                      aTGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCca                                 >  1:5442204/1‑71 (MQ=255)
                                      aTGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCca                                 >  1:3285736/1‑71 (MQ=255)
                                       tGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCcac                                <  1:5400436/71‑1 (MQ=255)
                                         cGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGaa                                                                <  1:3606104/37‑1 (MQ=255)
                                         cGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCcgc                                             <  1:3874169/56‑1 (MQ=255)
                                          ggTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAAc                             <  1:4748852/71‑1 (MQ=255)
                                           gTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCAtt                                       <  1:603351/60‑1 (MQ=255)
                                           gTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCAtt                                       <  1:593340/60‑1 (MQ=255)
                                           gTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCAtt                                       <  1:5330901/60‑1 (MQ=255)
                                           gTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCcac                                <  1:5342703/67‑1 (MQ=255)
                                           gTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCcac                                <  1:1541497/67‑1 (MQ=255)
                                           gTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCcac                                <  1:2913053/67‑1 (MQ=255)
                                            tGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAATTGGCGTg                                                       <  1:4648006/43‑1 (MQ=255)
                                            tGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTg                                                       <  1:80033/43‑1 (MQ=255)
                                            tGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTg                                                       <  1:4825754/43‑1 (MQ=255)
                                            tGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTg                                                       <  1:3872119/43‑1 (MQ=255)
                                            tGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTg                                                       <  1:1786805/43‑1 (MQ=255)
                                              cATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCa                          <  1:4491733/70‑1 (MQ=255)
                                                  aCGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGccc                                               >  1:4066239/1‑45 (MQ=255)
                                                   cgcgGTTGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATcaca                    <  1:1232952/71‑1 (MQ=255)
                                                   cgcgGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCAtt                                       <  1:1778596/52‑1 (MQ=255)
                                                        tggtgATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCcaca                               <  1:876268/55‑1 (MQ=255)
                                                        tggtgATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCcaca                               <  1:1449565/55‑1 (MQ=255)
                                                        tggtgATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCcaca                               <  1:2918272/55‑1 (MQ=255)
                                                        tggtgATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGa                >  1:4586885/1‑70 (MQ=255)
                                                         ggtgATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGa                >  1:2162000/1‑69 (MQ=255)
                                                            gATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCcga                                             >  1:1944767/1‑36 (MQ=255)
                                                              tGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCAtt                                       <  1:4597065/41‑1 (MQ=255)
                                                              tGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTc         >  1:5028019/1‑71 (MQ=255)
                                                              tGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTc         >  1:5302567/1‑71 (MQ=255)
                                                              tGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTc         >  1:4346728/1‑71 (MQ=255)
                                                               ggAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTcc        >  1:1844077/1‑71 (MQ=255)
                                                               ggAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTcc        >  1:3516891/1‑71 (MQ=255)
                                                                gaagaaGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCt       >  1:5507983/1‑71 (MQ=255)
                                                                gaagaaGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCt       <  1:2532897/71‑1 (MQ=255)
                                                                gaagaaGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCt       >  1:3298989/1‑71 (MQ=255)
                                                                gaagaaGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCt       >  1:4298450/1‑71 (MQ=255)
                                                                gaagaaGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCt       <  1:1566369/71‑1 (MQ=255)
                                                                gaagaaGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCt       <  1:4811083/71‑1 (MQ=255)
                                                                 aagaagTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCtg      <  1:3055171/71‑1 (MQ=255)
                                                                 aagaagTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCtg      <  1:1014573/71‑1 (MQ=255)
                                                                  agaagTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCa                          <  1:2554359/50‑1 (MQ=255)
                                                                     agTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCtgatga  <  1:2529385/71‑1 (MQ=255)
                                                                     agTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCtgatg   >  1:1131673/1‑70 (MQ=255)
                                                                     |                                                                      
TCAGCACGGGCCGTTCGCCTGGGGGAAAGATGCTCACGATGCGGTGCATAACGCGGTGGTGATGGAAGAAGTGGCGAAAATGGCGTGGATTGCCCGCGGCATTAACCCACAACTCAATCACATCGACAGCTTCCTGATGA  >  Exported/2872236‑2872375

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: