Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	313,534	A→G	7.5%	G185G (GGT→GGC)	acrA ←	multidrug efflux system

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	313,534	0	A	G	7.5%	265.9 / 8.8	93	G185G (GGT→GGC)	acrA	multidrug efflux system
Reads supporting (aligned to +/- strand):  ref base A (51/35);  new base G (2/5);  total (53/40)
Fisher's exact test for biased strand distribution p-value = 1.35e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.94e-01

GCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAG  >  Exported/313467‑313604                                                                    |                                                                       gctgcACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTAcc                                                                      <  1:540531/70‑1 (MQ=255) gctgcACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTAcc                                                                      <  1:3693696/70‑1 (MQ=255)    gcACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAAt                                                                   >  1:4312313/1‑70 (MQ=255)     cACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGc                                                                 >  1:5399426/1‑71 (MQ=255)      aCGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGc                                                                 >  1:1981536/1‑70 (MQ=255)      aCGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCg                                                                <  1:1841616/71‑1 (MQ=255)        ggTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGAc                                                              >  1:1172146/1‑71 (MQ=255)         gTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGc                                                                 <  1:1699255/67‑1 (MQ=255)         gTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGc                                                                 <  1:3544861/67‑1 (MQ=255)           tGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGa                                                               >  1:2772511/1‑67 (MQ=255)           tGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGa                                                               >  1:2442808/1‑67 (MQ=255)           tGCCAGCGCAGTCGCCTGACCGCTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGa                                                               >  1:3024802/1‑67 (MQ=255)             ccAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCaa                                                                    >  1:3156769/1‑60 (MQ=255)             ccAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTa                                                         <  1:5544201/71‑1 (MQ=255)               aGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAAt                                                       >  1:3808787/1‑71 (MQ=255)                gcgcAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATc                                                      >  1:5354033/1‑71 (MQ=255)                  gcAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATc                                                      >  1:3150097/1‑69 (MQ=255)                  gcAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCgg                                                    <  1:2841279/71‑1 (MQ=255)                   cAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGga                                                   <  1:2631577/71‑1 (MQ=255)                   cAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGA‑CGCTAATCGga                                                   <  1:4390026/70‑1 (MQ=255)                     gTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGga                                                   >  1:1120198/1‑69 (MQ=255)                        gCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAgg                                               <  1:4347586/70‑1 (MQ=255)                             aCCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCt                                                          <  1:5126496/54‑1 (MQ=255)                             aCCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGa                                            >  1:1387643/1‑68 (MQ=255)                             aCCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACtt                                         >  1:1000774/1‑71 (MQ=255)                             aCCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACt                                          <  1:4078213/70‑1 (MQ=255)                              ccGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCgg                                                    <  1:2058600/59‑1 (MQ=255)                              ccGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCgg                                                    <  1:2773031/59‑1 (MQ=255)                               cGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGc                                                                 >  1:2714395/1‑45 (MQ=255)                               cGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTaa                                                        >  1:1363686/1‑54 (MQ=255)                                gTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGgag                                                  >  1:2922155/1‑59 (MQ=255)                                 ttCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACttt                                        <  1:1507394/68‑1 (MQ=255)                                 ttCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACttt                                        <  1:3908390/68‑1 (MQ=255)                                 ttCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGgt                                     >  1:1044453/1‑71 (MQ=255)                                 ttCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGgt                                     >  1:4168834/1‑71 (MQ=255)                                 ttCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGgt                                     >  1:1079468/1‑71 (MQ=255)                                  tCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGgtg                                    >  1:278007/1‑71 (MQ=255)                                  tCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGgtg                                    >  1:284897/1‑71 (MQ=255)                                   cTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGgtg                                    >  1:3060659/1‑70 (MQ=255)                                      tACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGgtgt                                   <  1:3958640/68‑1 (MQ=255)                                      tACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTa                                  <  1:1234323/69‑1 (MQ=255)                                      tACCAATGCGCCTTCCGTCACGTGCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGgtgt                                   <  1:2560667/68‑1 (MQ=255)                                       aCCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGc                               >  1:4053291/1‑71 (MQ=255)                                          aaTGCGGCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAg                            <  1:4957902/71‑1 (MQ=255)                                            tGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGgtg                                    >  1:1280405/1‑61 (MQ=255)                                            tGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGgtg                                    >  1:2424949/1‑61 (MQ=255)                                             gcgcCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGga                                                   >  1:877363/1‑45 (MQ=255)                                              cgcCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGga                                                   >  1:609626/1‑44 (MQ=255)                                              cgcCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGga                                                   >  1:885729/1‑44 (MQ=255)                                              cgcCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGAtt                         >  1:5604312/1‑70 (MQ=255)                                              cgcCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGAtt                         >  1:1815974/1‑70 (MQ=255)                                              cgcCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGAtt                         <  1:555686/70‑1 (MQ=255)                                              cgcCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTg                        <  1:3291585/71‑1 (MQ=255)                                               gcCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTg                        <  1:345774/70‑1 (MQ=255)                                                 cTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATc                     >  1:5429045/1‑71 (MQ=255)                                                  ttCGGTCACAGTCGATTTGCCGATACGTCCGCTAATTGGCGCAGTGACTTTGGTATAAGc                               <  1:410310/60‑1 (MQ=255)                                                  ttCGGTCACAGTCGATTTGCCGATACGTCCGCTAATTGGCGCAGTGACTTTGGTATAAGc                               <  1:4492323/60‑1 (MQ=255)                                                  ttCGGTCACAGTCGATTTGCCGATACGTCCGCTAATTGGCGCAGTGACTTTGGTATAAGc                               <  1:5609237/60‑1 (MQ=255)                                                  ttCGGTCACAGTCGATTTGCCGATACGTCCGCTAATTGGCGCAGTGACTTTGGTATAAGCAAGATTGAt                      <  1:2698399/69‑1 (MQ=255)                                                  ttCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAg                            <  1:333139/63‑1 (MQ=255)                                                  ttCCGTCACGTTCGACTTACCAATACGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATc                     >  1:1768711/1‑70 (MQ=255)                                                   tcggTCACAGTCGATTTGCCGATACGTCCGCTAATTGGCGCAGTGACTTTGGTATAAGCAAGATTGa                       >  1:1674694/4‑67 (MQ=255)                                                   tcggTCACAGTCGATTTGCCGATACGTCCGCTAATTGGCGCAGTGACTTTGGTATAAGCAAGATTGa                       >  1:2488414/4‑67 (MQ=255)                                                    ccGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCcgc                  >  1:747901/1‑71 (MQ=255)                                                    ccGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCcgc                  >  1:5309105/1‑71 (MQ=255)                                                    ccGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCcg                   >  1:961809/1‑70 (MQ=255)                                                     cGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCcgcg                 <  1:2819622/71‑1 (MQ=255)                                                     cGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCcgcg                 >  1:3528495/1‑71 (MQ=255)                                                     cGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCcgcg                 <  1:2231851/71‑1 (MQ=255)                                                      gTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTg                                       <  1:4627500/48‑1 (MQ=255)                                                      gTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTg                                       <  1:1119593/48‑1 (MQ=255)                                                       tCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGgtgt                                   <  1:5474630/51‑1 (MQ=255)                                                       tCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCa               <  1:2370381/71‑1 (MQ=255)                                                        cACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGa                           >  1:105230/1‑58 (MQ=255)                                                        cACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAg              >  1:1840239/1‑71 (MQ=255)                                                         aCGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTg                        >  1:3201007/1‑60 (MQ=255)                                                         aCGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGAt                      >  1:5252971/1‑62 (MQ=255)                                                         aCGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGt             >  1:3249714/1‑71 (MQ=255)                                                         aCGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACATTGGTGTAAGcc                              >  1:378175/1‑54 (MQ=255)                                                          cGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACtt                                         <  1:5043541/42‑1 (MQ=255)                                                            ttCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGc                               <  1:4100273/50‑1 (MQ=255)                                                            ttCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTc          <  1:5258923/71‑1 (MQ=255)                                                             tCGATTTGCCGATACGTCCGCTAATTGGCGCAGTGACTTTGGTATAAGCAAGAtt                         <  1:905778/55‑1 (MQ=255)                                                             tCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGAtt                         >  1:3141002/1‑55 (MQ=255)                                                             tCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGAtt                         >  1:3422879/1‑55 (MQ=255)                                                             tCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGAtt                         >  1:3766842/1‑55 (MQ=255)                                                             tCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGAtt                         >  1:2593473/1‑55 (MQ=255)                                                             tCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGa                       >  1:3952600/1‑57 (MQ=255)                                                              cGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTg                                       >  1:5520484/1‑40 (MQ=255)                                                              cGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTg                                       >  1:28298/1‑40 (MQ=255)                                                              cGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCaa        >  1:717827/1‑71 (MQ=255)                                                                 cTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCa                             <  1:3053031/47‑1 (MQ=255)                                                                 cTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCTCGCAGTTTCAAc       <  1:3527718/69‑1 (MQ=255)                                                                    aCCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCaa        >  1:1506094/1‑65 (MQ=255)                                                                    aCCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCaa        >  1:2968328/1‑65 (MQ=255)                                                                    aCCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAg  >  1:1059598/1‑71 (MQ=255)                                                                    |                                                                       GCTGCACGGTTGCCAGCGCAGTCGCCTGACCGTTCTGTACCAATGCGCCTTCCGTCACGTTCGACTTACCAATGCGACCGCTAATCGGAGAGGTGACTTTGGTGTAAGCCAGATTGATCCGCGCAGTTTCAACGGCAG  >  Exported/313467‑313604

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑