Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,952,864 G→A 9.3% intergenic (‑173/+95) leuQ ← / ← rsmC tRNA‑Leu/16S RNA m2G1207 methylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,952,8640GA 9.3% 121.4 / 2.2 43intergenic (‑173/+95)leuQ/rsmCtRNA‑Leu/16S RNA m2G1207 methylase
Reads supporting (aligned to +/- strand):  ref base G (19/20);  new base A (2/2);  total (21/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAATGTCCATCAGGAAACTAGCGTGCAGGTTTGGTATGCATGCGGGGGCAGATGCCAGATGCGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATAAGGCGTTCACGCCGCA  >  Exported/2952795‑2952933
                                                                     |                                                                     
aaaTGTCCATCAGGAAACTAGCGTGCAGGTTTGGTATGCATGCGGGGGCAGATGCCAGATGCGACGCTGgc                                                                      <  1:3931403/71‑1 (MQ=255)
aaaTGTCCATCAGGAAACTAGCGTGCAGGTTTGGTATGCATGCGGGGGCAGATGCCAGATGCGACGCTGgc                                                                      >  1:1764167/1‑71 (MQ=255)
       cATCAGGAAACTAGCGTGCAGGTTTGGTATGCATGCGGGGGCAGATGCCAGATGCGACGCTGGCGCGTCtt                                                               <  1:3172218/71‑1 (MQ=255)
        aTCAGGAAACTAGCGTGCAGGTTTGGTATGCATGCGGGGGCAGATGCCAGATGCGACGCTGGCGCGTCTTa                                                              >  1:3616736/1‑71 (MQ=255)
        aTCAGGAAACTAGCGTGCAGGTTTGGTATGCATGCGGGGGCAGATGCCAGATGCGACGCTGGCGCGTCTTa                                                              >  1:1612340/1‑71 (MQ=255)
          cAGGAAACTAGCGTGCAGGTTTGGTATGCATGCGGTGGCAGATGCCAGATGCGACGCTGGCGCGTCTTAt                                                             >  1:4035718/1‑70 (MQ=255)
           aGGAAACTAGCGTGCAGGTTTGGTATGCATGCGGGGGCAGATGCCAGATGCGACGCTGGCGCGTCTTATCt                                                           <  1:1496207/71‑1 (MQ=255)
             gAAACTAGCGTGCAGGTTTGGTATGCATGCGGGGGCAGATGCCAGATGCGACGCTGGCGCGTCTTATCTgg                                                         >  1:5601990/1‑71 (MQ=255)
              aaaCTAGCGTGCAGGTTTGGTATGCATGCGGGGGCAGATGCCAGATGCGACGCTGGCGCGTCTTATCTGGc                                                        >  1:2654080/1‑71 (MQ=255)
               aaCTAGCGTGCAGGTTTGGTATGCATGCGGGGGCAGATGCCAGATGCGACGCTGGCGCGTCTTATCTGGcc                                                       >  1:3093730/1‑71 (MQ=255)
                    gCGTGCAGGTTTGGTATGCATGCGGGGGCAGATGCCAGATGCGACGCTGGCGCGTCTTATCTGGCCTACg                                                   >  1:824589/1‑70 (MQ=255)
                    gCGTGCAGGTTTGGTATGCATGCGGGGGCAGATGCCAGATGCGACGCTGGCGCGTCTTATCTGGCCTACg                                                   <  1:1793427/70‑1 (MQ=255)
                     cGTGCAGGTTTGGTATGCATGCGGGGGCAGATGCCAGATGCGACGCTGGCGCGTCTTATCTGGCCTACg                                                   >  1:4445936/1‑69 (MQ=255)
                          aGGTTTGGTATGCATGCGGGGGCAGATGCCAGATGCGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCt                                            <  1:1797335/71‑1 (MQ=255)
                          aGGTTTGGTATGCATGCGGGGGCAGATGCCAGATGCGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCt                                            <  1:1054707/71‑1 (MQ=255)
                           ggTTTGGTATGCATGCGGTGGCAGATGCCAGATGCGACGCTGGCGCGTCt                                                                <  1:5191639/50‑1 (MQ=255)
                                    tgcatgcGGTGGCAGATGCCAGATGCGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAg                                   <  1:5685050/70‑1 (MQ=255)
                                    tgcatgcGGGGGCGGATGCCAGATGCGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAgg                                  >  1:80829/1‑71 (MQ=255)
                                    tgcatgcGGGGGCAGATGCCAGATGCGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAgg                                  >  1:593047/1‑71 (MQ=255)
                                    tgcatgcGGGGGCAGATGCCAGATGCGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAgg                                  >  1:4770255/1‑71 (MQ=255)
                                      catgcGGGGGCAGATGCCAGATGCGACGCTGGCGCGTCt                                                                >  1:4773053/1‑39 (MQ=255)
                                      catgcGGGGGCAGATGCCAGATGCGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGtt                                >  1:1131947/1‑71 (MQ=255)
                                      catgcGGGGGCAGATGCCAGATGCGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGta                                >  1:1015345/1‑70 (MQ=255)
                                          cGGGGTCAGATGCCAGATGCGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTg                             <  1:3284808/70‑1 (MQ=255)
                                          cGGGGGCAGATGCCAGATGCGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTg                             <  1:1742415/70‑1 (MQ=255)
                                          cGGGGGCAGATGCCAGATGCGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGt                            <  1:4345284/71‑1 (MQ=255)
                                             gggCAGATGCCAGATGCGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGt                            <  1:3067244/68‑1 (MQ=255)
                                                 ttatgccagatgcGACGCTGACGCGTCTTATCTGGCCTACAAAGGGCTAACGTGCAGGttt                               <  1:3389087/59‑1 (MQ=18)
                                                 agatgccagatgcGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCg                      >  1:211415/1‑70 (MQ=255)
                                                  gatgccagatgcGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGa                    <  1:4228620/71‑1 (MQ=255)
                                                   atgccagatgcGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGa                    >  1:5637732/1‑70 (MQ=255)
                                                   atgccagatgcGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGAt                   <  1:5553128/71‑1 (MQ=255)
                                                     gccagatgcGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGAt                   >  1:1271032/1‑69 (MQ=255)
                                                      ccagatgcGACGCTGACGCGTCTTATCTGGCCTACAAAGGGCTAACGTGCAGGt                                 >  1:5415070/1‑54 (MQ=18)
                                                      ccagatgcGACGCTGACGCGTCTTATCTGGCCTACAAAGGGCTAACGTGCAGGt                                 >  1:4547068/1‑54 (MQ=18)
                                                        agatgcGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTg                             <  1:1369373/56‑1 (MQ=255)
                                                        agatgcGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTg                             <  1:1362622/56‑1 (MQ=255)
                                                         gatgcGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATAAGGCg             <  1:1307967/71‑1 (MQ=255)
                                                          atgcGACGCTGACGCGTCTTATCTGGCCTACAAAGGGCTAACGTGCAgg                                  <  1:3086673/49‑1 (MQ=18)
                                                           tgcGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATAAGGCGtt           <  1:1007146/71‑1 (MQ=255)
                                                           tgcGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATAAGGCGtt           <  1:5002663/71‑1 (MQ=255)
                                                           tgcGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATAAGGCGtt           >  1:1321382/1‑71 (MQ=255)
                                                           tgcGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATAAGGCGt            >  1:4442983/1‑70 (MQ=255)
                                                             cGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGtt                                <  1:3756413/48‑1 (MQ=255)
                                                             cGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATAAGGCGTTc          <  1:2463774/70‑1 (MQ=255)
                                                                     gcgcGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAgg                         <  1:5446323/47‑1 (MQ=255)
                                                                     gcgcGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATa                  <  1:4034977/54‑1 (MQ=255)
                                                                     gcgcGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATa                  <  1:3914212/54‑1 (MQ=255)
                                                                     gcgcGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATa                  <  1:3854165/54‑1 (MQ=255)
                                                                     gcgcGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATAAGGCGTTCACGCCGCa  <  1:1038300/70‑1 (MQ=255)
                                                                     |                                                                     
AAATGTCCATCAGGAAACTAGCGTGCAGGTTTGGTATGCATGCGGGGGCAGATGCCAGATGCGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATAAGGCGTTCACGCCGCA  >  Exported/2952795‑2952933

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: