Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 2,952,884 G→A 8.4% intergenic (‑193/+75) leuQ ← / ← rsmC tRNA‑Leu/16S RNA m2G1207 methylase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported2,952,8840GA 8.4% 206.5 / 5.8 71intergenic (‑193/+75)leuQ/rsmCtRNA‑Leu/16S RNA m2G1207 methylase
Reads supporting (aligned to +/- strand):  ref base G (30/35);  new base A (4/2);  total (34/37)
Fisher's exact test for biased strand distribution p-value = 4.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

GCGTGCAGGTTTGGTATGCATGCGGGGGCAGATGCCAGATGCGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACACGGTATTCGGCGA  >  Exported/2952815‑2952953
                                                                     |                                                                     
gCGTGCAGGTTTGGTATGCATGCGGGGGCAGATGCCAGATGCGACGCTGGCGCGTCTTATCTGGCCTACg                                                                       <  1:1793427/70‑1 (MQ=255)
gCGTGCAGGTTTGGTATGCATGCGGGGGCAGATGCCAGATGCGACGCTGGCGCGTCTTATCTGGCCTACg                                                                       >  1:824589/1‑70 (MQ=255)
 cGTGCAGGTTTGGTATGCATGCGGGGGCAGATGCCAGATGCGACGCTGGCGCGTCTTATCTGGCCTACg                                                                       >  1:4445936/1‑69 (MQ=255)
      aGGTTTGGTATGCATGCGGGGGCAGATGCCAGATGCGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCt                                                                <  1:1797335/71‑1 (MQ=255)
      aGGTTTGGTATGCATGCGGGGGCAGATGCCAGATGCGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCt                                                                <  1:1054707/71‑1 (MQ=255)
                tgcatgcGGTGGCAGATGCCAGATGCGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAg                                                       <  1:5685050/70‑1 (MQ=255)
                tgcatgcGGGGGCGGATGCCAGATGCGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAgg                                                      >  1:80829/1‑71 (MQ=255)
                tgcatgcGGGGGCAGATGCCAGATGCGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAgg                                                      >  1:4770255/1‑71 (MQ=255)
                tgcatgcGGGGGCAGATGCCAGATGCGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAgg                                                      >  1:593047/1‑71 (MQ=255)
                  catgcGGGGGCAGATGCCAGATGCGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGtt                                                    >  1:1131947/1‑71 (MQ=255)
                  catgcGGGGGCAGATGCCAGATGCGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGta                                                    >  1:1015345/1‑70 (MQ=255)
                      cGGGGTCAGATGCCAGATGCGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTg                                                 <  1:3284808/70‑1 (MQ=255)
                      cGGGGGCAGATGCCAGATGCGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTg                                                 <  1:1742415/70‑1 (MQ=255)
                      cGGGGGCAGATGCCAGATGCGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGt                                                <  1:4345284/71‑1 (MQ=255)
                         gggCAGATGCCAGATGCGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGt                                                <  1:3067244/68‑1 (MQ=255)
                             ttatgccagatgcGACGCTGACGCGTCTTATCTGGCCTACAAAGGGCTAACGTGCAGGttt                                                   <  1:3389087/59‑1 (MQ=18)
                             agatgccagatgcGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCg                                          >  1:211415/1‑70 (MQ=255)
                              gatgccagatgcGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGa                                        <  1:4228620/71‑1 (MQ=255)
                               atgccagatgcGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGa                                        >  1:5637732/1‑70 (MQ=255)
                               atgccagatgcGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGAt                                       <  1:5553128/71‑1 (MQ=255)
                                 gccagatgcGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGAt                                       >  1:1271032/1‑69 (MQ=255)
                                  ccagatgcGACGCTGACGCGTCTTATCTGGCCTACAAAGGGCTAACGTGCAGGt                                                     >  1:4547068/1‑54 (MQ=18)
                                  ccagatgcGACGCTGACGCGTCTTATCTGGCCTACAAAGGGCTAACGTGCAGGt                                                     >  1:5415070/1‑54 (MQ=18)
                                    agatgcGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTg                                                 <  1:1362622/56‑1 (MQ=255)
                                    agatgcGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTg                                                 <  1:1369373/56‑1 (MQ=255)
                                     gatgcGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATAAGGCg                                 <  1:1307967/71‑1 (MQ=255)
                                      atgcGACGCTGACGCGTCTTATCTGGCCTACAAAGGGCTAACGTGCAgg                                                      <  1:3086673/49‑1 (MQ=18)
                                       tgcGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATAAGGCGtt                               >  1:1321382/1‑71 (MQ=255)
                                       tgcGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATAAGGCGtt                               <  1:5002663/71‑1 (MQ=255)
                                       tgcGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATAAGGCGtt                               <  1:1007146/71‑1 (MQ=255)
                                       tgcGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATAAGGCGt                                >  1:4442983/1‑70 (MQ=255)
                                         cGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGtt                                                    <  1:3756413/48‑1 (MQ=255)
                                         cGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATAAGGCGTTc                              <  1:2463774/70‑1 (MQ=255)
                                                 gcgcGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAgg                                             <  1:5446323/47‑1 (MQ=255)
                                                 gcgcGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATa                                      <  1:4034977/54‑1 (MQ=255)
                                                 gcgcGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATa                                      <  1:3854165/54‑1 (MQ=255)
                                                 gcgcGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATa                                      <  1:3914212/54‑1 (MQ=255)
                                                 gcgcGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATAAGGCGTTCACGCCGCa                      <  1:1038300/70‑1 (MQ=255)
                                                   gcgTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGt                                                >  1:5554495/1‑42 (MQ=255)
                                                    cgTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGtttt                                                  <  1:2140421/39‑1 (MQ=255)
                                                    cgTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGtttt                                                  <  1:4633089/39‑1 (MQ=255)
                                                    cgTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGtttt                                                  <  1:2472352/39‑1 (MQ=255)
                                                    cgTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGttt                                                   >  1:4995822/1‑38 (MQ=255)
                                                    cgTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGttt                                                   >  1:573622/1‑38 (MQ=255)
                                                    cgTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGttt                                                   >  1:3259708/1‑38 (MQ=255)
                                                    cgTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGttt                                                   >  1:2762024/1‑38 (MQ=255)
                                                    cgTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGttt                                                   >  1:230646/1‑38 (MQ=255)
                                                    cgTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATAAGGCGTTCACGCCGCATcc                   <  1:46087/70‑1 (MQ=255)
                                                     gTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTg                                                 <  1:2737889/39‑1 (MQ=255)
                                                       cTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGaca               >  1:5426193/1‑71 (MQ=255)
                                                        ttATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAg                                              >  1:2438807/1‑39 (MQ=255)
                                                        ttATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAg                                              >  1:2291965/1‑39 (MQ=255)
                                                        ttATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGacac              >  1:200268/1‑71 (MQ=255)
                                                        ttATCTGGCCTACAAAGGGCTAACGTGCAGGtttt                                                  >  1:2938158/1‑35 (MQ=255)
                                                        ttATCTGGCCTACAAAGGGCTAACGTGCAGGtttt                                                  >  1:4719976/1‑35 (MQ=255)
                                                          aTCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATAAGGCGTTCa                             >  1:1459806/1‑54 (MQ=255)
                                                          aTCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGacac              <  1:2317889/69‑1 (MQ=255)
                                                           tCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACACgg            <  1:838632/70‑1 (MQ=255)
                                                           tCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACACgg            <  1:595978/70‑1 (MQ=255)
                                                             tGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACACGGTAt         >  1:2197231/1‑71 (MQ=255)
                                                               gCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATaa                                     >  1:2634651/1‑41 (MQ=255)
                                                                ccTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACACGGTa          >  1:4128494/1‑67 (MQ=255)
                                                                ccTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACACGGTa          >  1:4782658/1‑67 (MQ=255)
                                                                  tACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATAAGGCGTTCAcgcc                         <  1:5141599/50‑1 (MQ=255)
                                                                  tACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATAAGGCGTTCAcgcc                         <  1:110850/50‑1 (MQ=255)
                                                                   aagAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATAAGGc                                  >  1:2036014/3‑40 (MQ=255)
                                                                   aCGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACACGGTATTCGGc    >  1:5522408/1‑70 (MQ=255)
                                                                   aCGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACACGGTATTCGGc    >  1:4118069/1‑70 (MQ=255)
                                                                    cGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACACGGTa          >  1:1889811/1‑63 (MQ=255)
                                                                    cGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACACGGTATTCGGc    >  1:2485320/1‑69 (MQ=255)
                                                                    cGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACACGGTATTCGGc    >  1:2587815/1‑69 (MQ=255)
                                                                    cGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACACGGTATTCGGCga  <  1:5109667/71‑1 (MQ=255)
                                                                    cGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACACGGTATTCGGCga  <  1:2142700/71‑1 (MQ=255)
                                                                    cGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACACGGTATTCGGCga  <  1:3148350/71‑1 (MQ=255)
                                                                    cGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACACGGTATTCGGCga  <  1:4065407/71‑1 (MQ=255)
                                                                     |                                                                     
GCGTGCAGGTTTGGTATGCATGCGGGGGCAGATGCCAGATGCGACGCTGGCGCGTCTTATCTGGCCTACGAAGGGCTAACGTGCAGGTTTTGTAGGTCGGATAAGGCGTTCACGCCGCATCCGACACGGTATTCGGCGA  >  Exported/2952815‑2952953

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: