Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 313,864 A→G 17.4% I75I (ATT→ATC acrA ← multidrug efflux system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported313,8640AG17.4% 198.4 / 32.8 92I75I (ATT→ATCacrAmultidrug efflux system
Reads supporting (aligned to +/- strand):  ref base A (40/36);  new base G (10/6);  total (50/42)
Fisher's exact test for biased strand distribution p-value = 5.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.92e-01

AGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTG  >  Exported/313795‑313933
                                                                     |                                                                     
aGGATCAATCTGAAAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGTTTCAGGATaa                                                                      <  1:2256437/71‑1 (MQ=255)
    tCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATccc                                                                  >  1:3630095/1‑71 (MQ=255)
    tCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATcc                                                                   >  1:88970/1‑70 (MQ=255)
          tGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAAc                                                            >  1:5186021/1‑71 (MQ=255)
            aTAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTa                                                              <  1:472724/67‑1 (MQ=255)
            aTAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACtt                                                          <  1:4302614/71‑1 (MQ=255)
            aTAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACt                                                           <  1:4365912/70‑1 (MQ=255)
               gagagagaCACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAg                                                       <  1:3355624/71‑1 (MQ=255)
                agagagaCACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAgg                                                      <  1:2898845/71‑1 (MQ=255)
                    agaCACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAAt                                                                     >  1:4562670/1‑52 (MQ=255)
                    agaCACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGc                                                                >  1:3336319/1‑57 (MQ=255)
                      acacCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAAc                                                <  1:5298/71‑1 (MQ=255)
                         ccTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTg                                                         <  1:320484/59‑1 (MQ=255)
                         ccTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTg                                                         <  1:755252/59‑1 (MQ=255)
                         ccTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACg                                                   <  1:3472635/65‑1 (MQ=255)
                           tGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATaa                                                                      >  1:2894055/1‑44 (MQ=255)
                           tGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGa                                                     >  1:1226219/1‑61 (MQ=255)
                           tGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTc                                             <  1:390887/69‑1 (MQ=255)
                           tGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTc                                             <  1:268844/69‑1 (MQ=255)
                            gCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGaa                                                 >  1:3313676/1‑64 (MQ=255)
                            gCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGaa                                                 >  1:5434545/1‑64 (MQ=255)
                             cTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATaa                                                                      >  1:403855/1‑42 (MQ=255)
                             cTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATaa                                                                      >  1:5225794/1‑42 (MQ=255)
                             cTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTg                                           >  1:3209556/1‑69 (MQ=255)
                             cTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTg                                           >  1:4422835/1‑69 (MQ=255)
                             cTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTg                                           >  1:1906437/1‑69 (MQ=255)
                                cGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATc                                      >  1:4141687/1‑71 (MQ=255)
                                  acatCGCTGCCTTCAGTGAAATTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACt                                               <  1:5261606/57‑1 (MQ=255)
                                  acatCGCTGCCTTCAGTGAAATTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACt                                               <  1:4917131/57‑1 (MQ=255)
                                  aTGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAg                                                       <  1:3123355/52‑1 (MQ=255)
                                   tGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCgg                                   <  1:4271291/71‑1 (MQ=255)
                                    gTCGCTACCTTCTTTGAAATTACGCTTCAGGATaa                                                                      >  1:1187704/1‑35 (MQ=255)
                                    gTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGc                                                                >  1:4344865/1‑41 (MQ=255)
                                    gTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGc                                                                >  1:4953699/1‑41 (MQ=255)
                                    gTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCgg                                   >  1:4521836/1‑70 (MQ=255)
                                    atCGCTGCCTTCAGTGAAATTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCGGc                                          <  1:3393494/62‑1 (MQ=255)
                                     tCGCTGCCTTCAGTGAAATTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCGGCTATACgat                                  >  1:2845283/1‑68 (MQ=255)
                                       gCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACtt                                              <  1:2650266/56‑1 (MQ=255)
                                       gCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAgg                               >  1:4051084/1‑71 (MQ=255)
                                        cTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGc                              <  1:254615/71‑1 (MQ=255)
                                          aCCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGt                                  <  1:5230191/65‑1 (MQ=255)
                                          aCCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGt                                  <  1:1228423/65‑1 (MQ=255)
                                           ccTTCTTTGAAATTACGCTTCAGGATAATCCCGCt                                                               <  1:991978/35‑1 (MQ=255)
                                           ccTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAAc                                                <  1:5564794/50‑1 (MQ=255)
                                           ccTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAAc                                                <  1:2626268/50‑1 (MQ=255)
                                           ccTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACt                           >  1:1876190/1‑71 (MQ=255)
                                           ccTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACt                           >  1:1869642/1‑71 (MQ=255)
                                           ccTTCAGTGAAATTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCatt                           <  1:2516331/71‑3 (MQ=255)
                                             ttcttTGAAATTACGCTTCAGGATAATCCCGCTaa                                                             >  1:3772504/1‑35 (MQ=255)
                                             ttcttTGAAATTACGCTTCAGGATAATCCCGCTAACTTg                                                         <  1:5387738/39‑1 (MQ=255)
                                              tcttTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACt                                               >  1:1237771/1‑48 (MQ=255)
                                               cttTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACtt                                              >  1:3856700/1‑48 (MQ=255)
                                               cttTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACtt                                              >  1:4605907/1‑48 (MQ=255)
                                               cttTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTg                       >  1:3984667/1‑71 (MQ=255)
                                                tttGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGc                      >  1:513687/1‑71 (MQ=255)
                                                tttGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGc                      >  1:2760457/1‑71 (MQ=255)
                                                 ttGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATcc                                     <  1:2144178/55‑1 (MQ=255)
                                                 gtGAAATTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCTGCTATACGATAAGCATTGGTGCg                     <  1:2343094/70‑1 (MQ=255)
                                                  tGAAATTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCATTGGTGCgg                    >  1:1375392/1‑70 (MQ=255)
                                                   gAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGa                    <  1:5551224/70‑1 (MQ=255)
                                                    aaaTTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCATTGGt                        >  1:1981234/1‑65 (MQ=255)
                                                    aaaTTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCATTGGTg                       >  1:1572776/1‑66 (MQ=255)
                                                    aaaTTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCATTGGTg                       >  1:3001702/1‑66 (MQ=255)
                                                    aaaTTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCATTGGTg                       >  1:536100/1‑66 (MQ=255)
                                                    aaaTTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCATTGGTGCggc                   >  1:4205515/1‑68 (MQ=255)
                                                    aaaTTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCATTGGTGCGGcc                  >  1:2215143/1‑71 (MQ=255)
                                                    aaaTTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCATTGGTGCGGcc                  <  1:4455268/71‑1 (MQ=255)
                                                    aaaTTACTCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGAc                   <  1:5693475/70‑1 (MQ=255)
                                                    aaaTTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGAcc                  >  1:2856905/1‑71 (MQ=255)
                                                      aTTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCATTg                          >  1:5356662/1‑61 (MQ=255)
                                                      aTTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCATTg                          >  1:5135467/1‑61 (MQ=255)
                                                      aTTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCg                <  1:2536126/71‑1 (MQ=255)
                                                       ttACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCgg               <  1:4605918/71‑1 (MQ=255)
                                                       ttACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCgg               <  1:2171877/71‑1 (MQ=255)
                                                         aCGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCg                                    >  1:4701667/1‑48 (MQ=255)
                                                         aCGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCg                                    >  1:4708280/1‑48 (MQ=255)
                                                         aCGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCg                                    >  1:1544449/1‑48 (MQ=255)
                                                         aCGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGa              <  1:2308429/70‑1 (MQ=255)
                                                           gCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGc           >  1:139169/1‑71 (MQ=255)
                                                            cTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCt          <  1:1138572/71‑1 (MQ=255)
                                                              tCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCgg                                   <  1:2520358/44‑1 (MQ=255)
                                                              tCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTc         >  1:4285273/1‑70 (MQ=255)
                                                               cAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCgg       >  1:723581/1‑71 (MQ=255)
                                                               cAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCgg       >  1:3156673/1‑71 (MQ=255)
                                                               cAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCg        <  1:5147570/70‑1 (MQ=255)
                                                                aGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTa                                 >  1:1272766/1‑44 (MQ=255)
                                                                aGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTg                          >  1:887874/1‑51 (MQ=255)
                                                                aGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTg                          >  1:1081018/1‑51 (MQ=255)
                                                                aGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTg                          >  1:2580969/1‑51 (MQ=255)
                                                                 ggATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGtt     >  1:1133632/1‑71 (MQ=255)
                                                                  gATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTtg    <  1:663073/71‑1 (MQ=255)
                                                                    tAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCa                             >  1:5552838/1‑44 (MQ=255)
                                                                    tAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCa                             >  1:480781/1‑44 (MQ=255)
                                                                    tAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTtgtg  <  1:157456/71‑1 (MQ=255)
                                                                    tAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTtgtg  <  1:4781529/71‑1 (MQ=255)
                                                                    tAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTtgtg  <  1:4601654/71‑1 (MQ=255)
                                                                    tAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTtgtg  <  1:2803890/71‑1 (MQ=255)
                                                                     |                                                                     
AGGATCAATCTGATAGAGAGAGACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTG  >  Exported/313795‑313933

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: