Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 313,882 A→G 20.0% R69R (CGT→CGC acrA ← multidrug efflux system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported313,8820AG20.0% 186.0 / 41.5 95R69R (CGT→CGCacrAmultidrug efflux system
Reads supporting (aligned to +/- strand):  ref base A (39/37);  new base G (13/6);  total (52/43)
Fisher's exact test for biased strand distribution p-value = 2.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01

ACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAG  >  Exported/313817‑313943
                                                                 |                                                             
acacCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAAc                                                          <  1:5298/71‑1 (MQ=255)
   ccTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACg                                                             <  1:3472635/65‑1 (MQ=255)
     tGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGa                                                               >  1:1226219/1‑61 (MQ=255)
     tGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTc                                                       <  1:268844/69‑1 (MQ=255)
     tGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTc                                                       <  1:390887/69‑1 (MQ=255)
      gCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGaa                                                           >  1:5434545/1‑64 (MQ=255)
      gCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGaa                                                           >  1:3313676/1‑64 (MQ=255)
       cTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTg                                                     >  1:4422835/1‑69 (MQ=255)
       cTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTg                                                     >  1:3209556/1‑69 (MQ=255)
       cTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTg                                                     >  1:1906437/1‑69 (MQ=255)
          cGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATc                                                >  1:4141687/1‑71 (MQ=255)
            acatCGCTGCCTTCAGTGAAATTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACt                                                         <  1:5261606/57‑1 (MQ=255)
            acatCGCTGCCTTCAGTGAAATTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACt                                                         <  1:4917131/57‑1 (MQ=255)
             tGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCgg                                             <  1:4271291/71‑1 (MQ=255)
              gTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCgg                                             >  1:4521836/1‑70 (MQ=255)
              atCGCTGCCTTCAGTGAAATTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCGGc                                                    <  1:3393494/62‑1 (MQ=255)
               tCGCTGCCTTCAGTGAAATTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCGGCTATACgat                                            >  1:2845283/1‑68 (MQ=255)
                 gCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACtt                                                        <  1:2650266/56‑1 (MQ=255)
                 gCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAgg                                         >  1:4051084/1‑71 (MQ=255)
                  cTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGc                                        <  1:254615/71‑1 (MQ=255)
                    aCCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGt                                            <  1:1228423/65‑1 (MQ=255)
                    aCCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGt                                            <  1:5230191/65‑1 (MQ=255)
                     ccTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAAc                                                          <  1:5564794/50‑1 (MQ=255)
                     ccTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAAc                                                          <  1:2626268/50‑1 (MQ=255)
                     ccTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACt                                     >  1:1869642/1‑71 (MQ=255)
                     ccTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACt                                     >  1:1876190/1‑71 (MQ=255)
                     ccTTCAGTGAAATTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCatt                                     <  1:2516331/71‑3 (MQ=255)
                        tcttTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACt                                                         >  1:1237771/1‑48 (MQ=255)
                         cttTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACtt                                                        >  1:4605907/1‑48 (MQ=255)
                         cttTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACtt                                                        >  1:3856700/1‑48 (MQ=255)
                         cttTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTg                                 >  1:3984667/1‑71 (MQ=255)
                          tttGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGc                                >  1:2760457/1‑71 (MQ=255)
                          tttGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGc                                >  1:513687/1‑71 (MQ=255)
                           ttGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATcc                                               <  1:2144178/55‑1 (MQ=255)
                           gtGAAATTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCTGCTATACGATAAGCATTGGTGCg                               <  1:2343094/70‑1 (MQ=255)
                            tGAAATTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCATTGGTGCgg                              >  1:1375392/1‑70 (MQ=255)
                             gAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGa                              <  1:5551224/70‑1 (MQ=255)
                              aaaTTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCATTGGt                                  >  1:1981234/1‑65 (MQ=255)
                              aaaTTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCATTGGTg                                 >  1:3001702/1‑66 (MQ=255)
                              aaaTTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCATTGGTg                                 >  1:536100/1‑66 (MQ=255)
                              aaaTTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCATTGGTg                                 >  1:1572776/1‑66 (MQ=255)
                              aaaTTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCATTGGTGCggc                             >  1:4205515/1‑68 (MQ=255)
                              aaaTTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCATTGGTGCGGcc                            <  1:4455268/71‑1 (MQ=255)
                              aaaTTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCATTGGTGCGGcc                            >  1:2215143/1‑71 (MQ=255)
                              aaaTTACTCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGAc                             <  1:5693475/70‑1 (MQ=255)
                              aaaTTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGAcc                            >  1:2856905/1‑71 (MQ=255)
                                aTTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCATTg                                    >  1:5356662/1‑61 (MQ=255)
                                aTTGCGATTCAGTACGATCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCATTg                                    >  1:5135467/1‑61 (MQ=255)
                                aTTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCg                          <  1:2536126/71‑1 (MQ=255)
                                 ttACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCgg                         <  1:2171877/71‑1 (MQ=255)
                                 ttACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCgg                         <  1:4605918/71‑1 (MQ=255)
                                   aCGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCg                                              >  1:1544449/1‑48 (MQ=255)
                                   aCGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCg                                              >  1:4708280/1‑48 (MQ=255)
                                   aCGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCg                                              >  1:4701667/1‑48 (MQ=255)
                                   aCGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGa                        <  1:2308429/70‑1 (MQ=255)
                                     gCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGc                     >  1:139169/1‑71 (MQ=255)
                                      cTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCt                    <  1:1138572/71‑1 (MQ=255)
                                        tCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCgg                                             <  1:2520358/44‑1 (MQ=255)
                                        tCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTc                   >  1:4285273/1‑70 (MQ=255)
                                         cAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCgg                 >  1:3156673/1‑71 (MQ=255)
                                         cAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCgg                 >  1:723581/1‑71 (MQ=255)
                                         cAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCg                  <  1:5147570/70‑1 (MQ=255)
                                          aGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTa                                           >  1:1272766/1‑44 (MQ=255)
                                          aGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTg                                    >  1:887874/1‑51 (MQ=255)
                                          aGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTg                                    >  1:1081018/1‑51 (MQ=255)
                                          aGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTg                                    >  1:2580969/1‑51 (MQ=255)
                                           ggATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGtt               >  1:1133632/1‑71 (MQ=255)
                                            gATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTtg              <  1:663073/71‑1 (MQ=255)
                                              tAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCa                                       >  1:5552838/1‑44 (MQ=255)
                                              tAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCa                                       >  1:480781/1‑44 (MQ=255)
                                              tAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTtgtg            <  1:4601654/71‑1 (MQ=255)
                                              tAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTtgtg            <  1:157456/71‑1 (MQ=255)
                                              tAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTtgtg            <  1:4781529/71‑1 (MQ=255)
                                              tAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTtgtg            <  1:2803890/71‑1 (MQ=255)
                                                aTCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGa           <  1:28518/70‑1 (MQ=255)
                                                aTCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCATTg                                    >  1:1602933/1‑45 (MQ=255)
                                                aTCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCATTg                                    >  1:3751927/1‑45 (MQ=255)
                                                aTCCCGCTAACCTGTGGGCGAACTTCGGCTATACGATAAGCATTg                                    >  1:180262/1‑45 (MQ=255)
                                                 tCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTa                                           >  1:999242/1‑37 (MQ=255)
                                                 tCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTa                                           >  1:4622318/1‑37 (MQ=255)
                                                   ccGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTg       >  1:4630114/1‑71 (MQ=255)
                                                     gCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCa     <  1:5687358/71‑1 (MQ=255)
                                                      cTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCag    <  1:2953582/71‑1 (MQ=255)
                                                      cTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCag    <  1:2961360/71‑1 (MQ=255)
                                                      cTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCag    <  1:4744225/71‑1 (MQ=255)
                                                       tAACTTGAGGAGGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTtgtg            <  1:4120954/62‑1 (MQ=255)
                                                       tAACTTGAGGACGAACTTCTGCGATCCGGTAGGCa                                       >  1:2529336/1‑35 (MQ=255)
                                                       tAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGtt               >  1:464566/1‑59 (MQ=255)
                                                       tAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTtgtg            <  1:2541091/62‑1 (MQ=255)
                                                       tAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTtgtg            <  1:1502636/62‑1 (MQ=255)
                                                        aaCTTGAGGACGAACTTCTGCGGTCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCagag  >  1:5040791/1‑71 (MQ=255)
                                                        aaCTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTtgtg            <  1:2107542/61‑1 (MQ=255)
                                                          cTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGAt          >  1:4652778/1‑61 (MQ=255)
                                                           ttGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTtgtg            <  1:1048885/58‑1 (MQ=255)
                                                             gAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGAt          >  1:4345115/1‑58 (MQ=255)
                                                                gACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCag    <  1:2579096/61‑1 (MQ=255)
                                                                 |                                                             
ACACCTGCTTCGATGTCGCTACCTTCTTTGAAATTACGCTTCAGGATAATCCCGCTAACTTGAGGACGAACTTCTGCGATCCGGTAGGCACTGGTGCGACCCGGAAGCTCGGTTGTGATCTGCAGAG  >  Exported/313817‑313943

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: