BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	132,223	T→A	21.9%	I172I (ATT→ATA)	yadD →	predicted transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	132,223	0	T	A	21.9%	215.2 / 57.6	105	I172I (ATT→ATA)	yadD	predicted transposase
Reads supporting (aligned to +/- strand): ref base T (37/45); new base A (15/8); total (52/53)
Fisher's exact test for biased strand distribution p-value = 1.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 106 total aligned reads displayed.

ATAACAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACT  >  Exported/132153‑132288

ataACAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGAtt                                                                   >  1:4143045/1‑71 (MQ=255)

   aCAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGc                                                                 <  1:2161053/70‑1 (MQ=255)

   aCAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGc                                                                 <  1:3522708/70‑1 (MQ=255)

    cAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGa                                                               <  1:1986915/71‑1 (MQ=255)

         cTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATtctc                                                          <  1:1800412/71‑1 (MQ=255)

          tttCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATtctc                                                          >  1:648428/1‑70 (MQ=255)

           ttCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCTACATCGGCGGATTGCGATTCTCg                                                         >  1:2903375/1‑70 (MQ=255)

            tCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGaa                                                       >  1:5084483/1‑71 (MQ=255)

             cccGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCg                                                         <  1:4883306/68‑1 (MQ=255)

              ccGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAac                                                      <  1:4287679/70‑1 (MQ=255)

               cGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAac                                                      <  1:3269773/69‑1 (MQ=255)

                 cTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAacta                                                    >  1:1291540/1‑69 (MQ=255)

                 cTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAacta                                                    >  1:5320433/1‑69 (MQ=255)

                    gtggATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCa                                               <  1:5028866/71‑1 (MQ=255)

                       gATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaaa                                            <  1:2104096/71‑1 (MQ=255)

                       gATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaaa                                            <  1:3186170/71‑1 (MQ=255)

                       gATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaa                                             <  1:5527922/70‑1 (MQ=255)

                        atatCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaaa                                            <  1:2659004/70‑1 (MQ=255)

                           taaCCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAacta                                                    >  1:3366443/3‑59 (MQ=255)

                           tCACCATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGct                                                                >  1:1639548/1‑46 (MQ=255)

                           tCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAacta                                                    >  1:1715133/1‑59 (MQ=255)

                           tCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAacta                                                    >  1:3654363/1‑59 (MQ=255)

                           tCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAacta                                                    >  1:5194850/1‑59 (MQ=255)

                            cACCATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCata                                       >  1:4456591/1‑71 (MQ=255)

                            cACCATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCat                                        >  1:3151006/1‑70 (MQ=255)

                            cACCATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCat                                        >  1:5139991/1‑70 (MQ=255)

                            cACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACata                                       >  1:137935/1‑71 (MQ=255)

                             aCCATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCata                                       <  1:1682564/70‑1 (MQ=255)

                             aCCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACatat                                      <  1:5411175/71‑1 (MQ=255)

                                aTCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCg                                   <  1:4109234/71‑1 (MQ=255)

                                 tCACAGCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCg                                                         <  1:793773/48‑1 (MQ=255)

                                 tCACACCGGATGACGAAATCATGCAACATCGGCGGATTg                                                                  >  1:2371299/1‑39 (MQ=255)

                                 tCACACCGGATGACGAAATCATGCAACATCGGCGGATTg                                                                  >  1:3622995/1‑39 (MQ=255)

                                 tCACACCGGATGACGAAATCATGCAACATCGGCGGATTg                                                                  >  1:1589802/1‑39 (MQ=255)

                                 tCACACCGGATGACGAAATCATGCAACATCGGCGGATTg                                                                  >  1:2733784/1‑39 (MQ=255)

                                 tCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAacta                                                    <  1:1364574/53‑1 (MQ=255)

                                 tCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGc                                  <  1:3733406/71‑1 (MQ=255)

                                 tCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGc                                  <  1:2811198/71‑1 (MQ=255)

                                 tCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGc                                  >  1:1724271/1‑71 (MQ=255)

                                 tCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGc                                  <  1:1990572/71‑1 (MQ=255)

                                 tCACACCGGATGACGAAATCATGCAACATCGGAGGATTGCGATTCTCg                                                         <  1:3171776/48‑1 (MQ=255)

                                  cacaCCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGaa                                                       >  1:1119891/1‑49 (MQ=255)

                                  cacaCCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGaa                                                       >  1:283599/1‑49 (MQ=255)

                                  cacaCCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGaa                                                       >  1:1469273/1‑49 (MQ=255)

                                  cacaCCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGa                                                        >  1:824333/1‑48 (MQ=255)

                                   acacCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGcc                                 <  1:5225852/70‑1 (MQ=255)

                                   acacCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGcc                                 <  1:1456262/70‑1 (MQ=255)

                                     acCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaaa                                            <  1:4922131/57‑1 (MQ=255)

                                     acCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaaa                                            <  1:1591358/57‑1 (MQ=255)

                                       cGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAgcgc                            <  1:1982600/71‑1 (MQ=255)

                                       cGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAgcgc                            >  1:5603944/1‑71 (MQ=255)

                                       cGGATGACGAAATCATGCAACATAGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAgcg                             >  1:4314735/1‑70 (MQ=255)

                                        ggATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACa                                         <  1:4411448/57‑1 (MQ=255)

                                        ggATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACa                                         <  1:5499548/57‑1 (MQ=255)

                                          aTGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaat                                            <  1:1765041/52‑2 (MQ=255)

                                           tGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAac                                                      <  1:5469016/41‑1 (MQ=255)

                                           tGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAac                                                      <  1:2405323/41‑1 (MQ=255)

                                           tGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAac                                                      <  1:3950972/41‑1 (MQ=255)

                                           tGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAac                                                      <  1:1419189/41‑1 (MQ=255)

                                           tGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGc                                  <  1:5480886/61‑1 (MQ=255)

                                           tGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACt                        <  1:5105414/71‑1 (MQ=255)

                                             aCGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACtt                       >  1:4311932/1‑70 (MQ=255)

                                                 aaTCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGt                  <  1:4346016/71‑1 (MQ=255)

                                                  aTCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGCGACcgcg                           <  1:1981471/61‑1 (MQ=255)

                                                  aTCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGCGACCGCGATTTAAtcgg                 <  1:3144933/71‑4 (MQ=255)

                                                   tCATGCAACATCGGCGTATAGCTCTGCTTGAACTGATTCAAAAGCATATTCGCGACCGCGat                         <  1:437409/62‑2 (MQ=255)

                                                   tCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGCGACCGCGat                         <  1:5381492/62‑2 (MQ=255)

                                                   tCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGCGACCGCGat                         <  1:3389478/62‑2 (MQ=255)

                                                   tCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGCGACCGCGAttt                       <  1:4614380/64‑1 (MQ=255)

                                                   tCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGCGACCGCGATTTAAtcgg                 <  1:4086207/70‑4 (MQ=255)

                                                   tCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaaaa                                           <  1:2386059/44‑1 (MQ=255)

                                                   tCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaaaa                                           <  1:5304759/44‑1 (MQ=255)

                                                   tCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaaaa                                           <  1:3964826/44‑1 (MQ=255)

                                                    cATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCata                                       >  1:1096838/1‑47 (MQ=255)

                                                    cATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCat                                        >  1:3007904/1‑46 (MQ=255)

                                                    cATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCat                                        >  1:3643736/1‑46 (MQ=255)

                                                    cATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCat                                        >  1:3949494/1‑46 (MQ=255)

                                                    cATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGCGACCGCGat                         >  1:2371657/1‑60 (MQ=255)

                                                    cATGCAACATAGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGCGACCGCGat                         >  1:3248330/1‑60 (MQ=255)

                                                     aTGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACa                                         >  1:176267/1‑44 (MQ=255)

                                                      tGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaa                                             <  1:202621/39‑1 (MQ=255)

                                                       gCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGcga                                >  1:2947347/1‑49 (MQ=255)

                                                        cAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAgcg                             >  1:1678405/1‑53 (MQ=255)

                                                        cAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCt           <  1:4014833/71‑1 (MQ=255)

                                                        cAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCt           >  1:121453/1‑71 (MQ=255)

                                                         aaCATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGCGACCGCGATTTa                      >  1:2398642/1‑59 (MQ=255)

                                                         aaCATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGCGACCGCGATTTa                      >  1:2550432/1‑59 (MQ=255)

                                                          aCATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAgcgc                            <  1:825848/52‑1 (MQ=255)

                                                            aTCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTaa                     >  1:5389881/1‑57 (MQ=255)

                                                            aTCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTaa                     >  1:4756399/1‑57 (MQ=255)

                                                            aTCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAg       >  1:3152257/1‑71 (MQ=255)

                                                              cggcggATTGCGATTCTCGAACTACTGCAAAAACatat                                      >  1:2487730/1‑38 (MQ=255)

                                                              cggcggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAAt                    >  1:5388259/1‑56 (MQ=255)

                                                              cggcggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAAt                    >  1:4733468/1‑56 (MQ=255)

                                                              cggcggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAAt                    >  1:4727016/1‑56 (MQ=255)

                                                               ggcggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCaa    <  1:1726524/71‑1 (MQ=255)

                                                               ggcggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCaa    >  1:549698/1‑71 (MQ=255)

                                                               ggcggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCaa    >  1:5025523/1‑71 (MQ=255)

                                                               ggcggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCaa    <  1:1932043/71‑1 (MQ=255)

                                                                  ggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACt  <  1:1854585/70‑1 (MQ=255)

ATAACAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACT  >  Exported/132153‑132288

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`