Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 325,034 G→T 5.3% S482S (TCG→TCT htpG → molecular chaperone HSP90 family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported325,0340GT 5.3% 304.6 / 2.2 93S482S (TCG→TCThtpGmolecular chaperone HSP90 family
Reads supporting (aligned to +/- strand):  ref base G (41/47);  new base T (3/2);  total (44/49)
Fisher's exact test for biased strand distribution p-value = 6.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.13e-01

Only 100 of 102 total aligned reads displayed.

CTTTCCGACCGCATCGATGAGTGGATGATGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCTTGAAAAACTGGCTGACGAAGTTGATGAGAGCGCGAAAGAAGCGGAG  >  Exported/324966‑325103
                                                                    |                                                                     
ctTTCCGACCGCATCGATGAGTGGATGATGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCgg                                                                      >  1:414338/1‑70 (MQ=255)
ctTTCCGACCGCATCGATGAGTGGATGATGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGgt                                                                     >  1:4479922/1‑71 (MQ=255)
  ttCCGACCGCATCGATGAGTGGATGATGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGgtgt                                                                   >  1:4929981/1‑71 (MQ=255)
  ttCCGACCGCATCGATGAGTGGATGATGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGgtgt                                                                   >  1:4750449/1‑71 (MQ=255)
     cGACCGCATCGATGAGTGGATGATGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGGTGTCTa                                                                <  1:3754812/71‑1 (MQ=255)
     cGACCGCATCGATGAGTGGATGATGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGGTGTCTa                                                                <  1:4215588/71‑1 (MQ=255)
     cGACCGCATCGATGAGTGGATGATGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGGTGTCTa                                                                <  1:476906/71‑1 (MQ=255)
     cGACCGCATCGATGAGTGGATGATGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGGTGTCTa                                                                <  1:2172347/71‑1 (MQ=255)
       aCCGCATCGATGAGTGGATGATGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGGTGTCTaaa                                                              <  1:1970939/71‑1 (MQ=255)
       aCCGCATCGATGAGTGGATGATGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGGTGTCTa                                                                >  1:4703651/1‑69 (MQ=255)
        ccGCATCGATGAGTGGATGATGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGGTGTCTaaa                                                              <  1:4444261/70‑1 (MQ=255)
        ccGCATCGATGAGTGGATGATGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGGTGTCTAAAg                                                             <  1:4233318/71‑1 (MQ=255)
          gCATCGATGAGTGGATGATGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGGTGTCTAAAGtt                                                           <  1:5202696/71‑1 (MQ=255)
          gCATCGATGAGTGGATGATGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGGTGTCTAAAGtt                                                           <  1:2110772/71‑1 (MQ=255)
          gCATCGATGAGTGGATGATGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGGTGTCTAAAGtt                                                           <  1:3011042/71‑1 (MQ=255)
           cATCGATGAGTGGATGATGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGgt                                                                     >  1:1499357/1‑60 (MQ=255)
           cATCGATGAGTGGATGATGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGgt                                                                     >  1:5376689/1‑60 (MQ=255)
           cATCGATGAGTGGATGATGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGgt                                                                     >  1:3767965/1‑60 (MQ=255)
             tCGATGAGTGGATGATGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCg                                                                       >  1:1012235/1‑56 (MQ=255)
             tCGATGAGTGGATGATGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCg                                                                       >  1:2020001/1‑56 (MQ=255)
             tCGATGAGTGGATGATGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCg                                                                       >  1:612703/1‑56 (MQ=255)
             tCGATGAGTGGATGATGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCg                                                                       >  1:1591517/1‑56 (MQ=255)
             tCGATGAGTGGATGATGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGGTGTCTaa                                                               <  1:2513710/64‑1 (MQ=255)
             tCGATGAGTGGATGATGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGGTGTCTAAAGTTGAc                                                        <  1:4977268/71‑1 (MQ=255)
             tCGATGAGTGGATGATGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGGTGTCTAAAGTTGAc                                                        <  1:769878/71‑1 (MQ=255)
               gATGAGTGGATGATGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCgg                                                                      >  1:3739541/1‑55 (MQ=255)
               gATGAGTGGATGATGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGGTGTCTAAAGt                                                            >  1:3040560/1‑65 (MQ=255)
               gATGAGTGGATGATGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGGTGTCTAAAGTTGa                                                         >  1:1891137/1‑68 (MQ=255)
               gATGAGTGGATGATGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGGTGTCTAAAGTTGa                                                         >  1:3278479/1‑68 (MQ=255)
               gATGAGTGGATGATGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGGTGTCTAAAGTTGAc                                                        >  1:5078307/1‑69 (MQ=255)
               gATGAGTGGATGATGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGGTGTCTAAAGTTGAc                                                        >  1:2550211/1‑69 (MQ=255)
               gATGAGTGGATGATGAACTATCTGACTGAGTTCGACGGTAAAACGTTCCAGTCGGTGTCTAAAGTTGAc                                                        >  1:3206269/1‑69 (MQ=255)
                   aGTGGATGATGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGGTGTCTAAAGTTGACGAGTc                                                   <  1:276366/70‑1 (MQ=255)
                     tGGATGATGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCg                                                                       <  1:2076086/48‑1 (MQ=255)
                     tGGATGATGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCg                                                                       <  1:3934528/48‑1 (MQ=255)
                       gatgatGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGgt                                                                     >  1:1910171/1‑48 (MQ=255)
                       gatgatGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGgt                                                                     >  1:1940470/1‑48 (MQ=255)
                       gatgatGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGgt                                                                     >  1:1337967/1‑48 (MQ=255)
                       gatgatGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGgt                                                                     >  1:695461/1‑48 (MQ=255)
                       gatgatGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCtt                                               >  1:2007859/1‑70 (MQ=255)
                       gatgatGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCTTg                                              <  1:5122867/71‑1 (MQ=255)
                          gatgaACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGGTGTCTAAAGTTGACGa                                                      <  1:286094/60‑1 (MQ=255)
                          gatgaACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGGTGTCTAAAGTTGACGa                                                      <  1:1870240/60‑1 (MQ=255)
                           atgaACTATCTGACTGAGTTCGACGGTAAACCGTTCCATTCGGTGTCTAAAGTTGACGAGTCGCTTGaaa                                           <  1:2475267/70‑1 (MQ=255)
                           atgaACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCTTGaaaa                                          <  1:4144955/71‑1 (MQ=255)
                           atgaACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCTTGaaa                                           <  1:1321471/70‑1 (MQ=255)
                           atgaACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCTTGaaa                                           <  1:2494102/70‑1 (MQ=255)
                           atgaACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCTTGaaa                                           <  1:5098741/70‑1 (MQ=255)
                           atgaACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCTTGaaa                                           <  1:2187931/70‑1 (MQ=255)
                            tgaACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGGTGTCTAAAGTTGACGAGt                                                    >  1:5666234/1‑60 (MQ=255)
                                 tATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCTTGaaa                                           <  1:3476612/64‑1 (MQ=255)
                                 tATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCTTGaaa                                           <  1:1313241/64‑1 (MQ=255)
                                   tCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGGTGTCTAAAGtt                                                           <  1:4266066/46‑1 (MQ=255)
                                   tCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCt                                                >  1:2653310/1‑57 (MQ=255)
                                   tCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCt                                                >  1:5380774/1‑57 (MQ=255)
                                    ctgactgaGTTCGACGGTAAACCGTTCCAGTCGgtgt                                                                   <  1:3630192/37‑1 (MQ=255)
                                     tgactgaGTTCGACGGTAAACCGTTCCAGTCGGTGTCTAAAGTTGa                                                         >  1:5415853/1‑46 (MQ=255)
                                     tgactgaGTTCGACGGTAAACCGTTCCAGTCGGTGTCTAAAGTTGa                                                         >  1:5101605/1‑46 (MQ=255)
                                     tgactgaGTTCGACGGTAAACCGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCTTGAAAAACTGGCTGAc                                >  1:4050161/1‑71 (MQ=255)
                                       actgaGTTCGACGGTAAACCGTTCCAGTCGGTGTCTa                                                                <  1:2210341/37‑1 (MQ=255)
                                       actgaGTTCGACGGTAAACCGTTCCAGTCGGTGTCTa                                                                <  1:2445211/37‑1 (MQ=255)
                                       actgaGTTCGACGGTAAACCGTTCCAGTCGGTGTCTa                                                                <  1:3260398/37‑1 (MQ=255)
                                        ctgaGTTCGACGGTAAACCGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCTTGaaaa                                          <  1:3185125/58‑1 (MQ=255)
                                         tgaGTTCGACGGTAAACCGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCTTGaaaa                                          <  1:2888715/57‑1 (MQ=255)
                                           aGTTCGACGGTAAACCGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCTTGAAAAACTGGCTGACGAAGtt                          >  1:1752657/1‑71 (MQ=255)
                                           aGTTCGACGGTAAACCGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCTTGAAAAACTGGCTGACGAAGtt                          >  1:2011152/1‑71 (MQ=255)
                                           aGTTCGACGGTAAACCGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCTTGAAAAACTGGCTGACGAAGtt                          <  1:2116836/71‑1 (MQ=255)
                                            gTTCGACTGTAAACCGGTCCAGTCGGTGTCTAAAGTTGACGAGTCGCTTGAAAAACTGGCGGACGAAGTtg                         <  1:138475/71‑1 (MQ=255)
                                            gTTCGACGGTAAACCGTTCCAGTCGGTGTCTAAAGtt                                                           <  1:279424/37‑1 (MQ=255)
                                            gTTCGACGGTAAACCGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCTTGAAAAACTGGCTGACGAAGtt                          >  1:4633751/1‑70 (MQ=255)
                                             ttCGACGGTAAACCGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCTTGAAAAACTGGCTGACGAAGTtg                         <  1:551334/70‑1 (MQ=255)
                                              tCGACGGTAAACCGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCTTGAAAAACTGGCTGACGAAGTtgat                       >  1:5686805/1‑71 (MQ=255)
                                               cGACGGTAAACCGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCTTGAAAAACTGGCTGACGAAGTtgat                       <  1:1228622/70‑1 (MQ=255)
                                               cGACGGTAAACCGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCTTGAAAAACTGGCTGACGAAGTtgat                       <  1:4056530/70‑1 (MQ=255)
                                               cGACGGTAAACCGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCTTGAAAAACTGGCTGACGAAGTtgat                       <  1:1848283/70‑1 (MQ=255)
                                                gACGGTAAACCGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCTTGAAAAACTGGCTGACGAAGTtgacga                     <  1:4499850/71‑1 (MQ=255)
                                                  cGGTAAACCGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCTTGAAAAACTGGCTGACGAAGTtgatga                     >  1:1319304/1‑69 (MQ=255)
                                                    gTAAACCGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCTTGaaaaa                                         <  1:4206105/47‑1 (MQ=255)
                                                    gTAAACCGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCTTGAAAAACTGGCTGACGAAGTtgat                       <  1:4528821/65‑1 (MQ=255)
                                                    gTAAACCGTTCCAGTCGGTGTCTAAAGCTGACGAGTCGCTTGaaaaa                                         <  1:2295609/47‑1 (MQ=255)
                                                     tAAACCGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCTTGAAAAACTGGCTGACGAAGTtga                        <  1:2461823/63‑1 (MQ=255)
                                                      aaaCCGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCTTg                                              >  1:2822566/1‑40 (MQ=255)
                                                      aaaCCGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCTTGAAAAACt                                       >  1:576699/1‑47 (MQ=255)
                                                      aaaCCGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCTTGAAAAACTGGCTGACg                               >  1:4614711/1‑55 (MQ=255)
                                                       aaCCGTTCCAGTCTGTGTCTAAAGTTGACGAGTCGCtg                                               >  1:2888440/1‑37 (MQ=255)
                                                       aaCCGTTCCAGTCTGTGTCTAAAGTTGACGAGTCGCtg                                               >  1:683448/1‑37 (MQ=255)
                                                       aaCCGTTCCAGTCTGTGTCTAAAGTTGACGAGTCGCtg                                               >  1:5188630/1‑37 (MQ=255)
                                                       aaCCGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCTTGAAAAAc                                        >  1:3608674/1‑45 (MQ=255)
                                                       aaCCGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCTTGAAAAAc                                        >  1:5217936/1‑45 (MQ=255)
                                                       aaCCGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCTTGAAAAAc                                        >  1:2147599/1‑45 (MQ=255)
                                                         ccGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCTTGaaaaa                                         >  1:3917486/1‑42 (MQ=255)
                                                         ccGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCTTGa                                             >  1:1679360/1‑38 (MQ=255)
                                                         ccGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCTTGa                                             >  1:3731245/1‑38 (MQ=255)
                                                            ttCCAGTCTGTGTCTAAAGTTGACGAGTCTCTGGAAAAACTGGCTGACGAAGTTGATGAGAGCGCGa             <  1:2285955/67‑1 (MQ=255)
                                                            ttCCAGTCTGTGTCTAAAGTTGACGAGTCGCTGGAAAAACTGGCTGACGAAGTTGATGAGAGCGCGa             <  1:5289291/67‑1 (MQ=255)
                                                            ttCCAGTCGGTGTCTAAAGTTGACGAGTCGCTTGAAAAACTGGCTGACGAAGTTGATGAGAGCGCGAaaga         <  1:5071815/71‑1 (MQ=255)
                                                               cAGTCGGTGTCTAAAGTTGACGAGTCGCTTGAAAAACTGGCTGACGAAGtt                          <  1:1056041/51‑1 (MQ=255)
                                                               cAGTCGGTGTCTAAAGTTGACGAGTCGCTTGAAAAACTGGCTGACGAAGTTGATGAGAGCGCGAAAGAAGc      <  1:1479284/71‑1 (MQ=255)
                                                                aGTCGGTGTCTAAAGTTGACGAGTCGCTTGAAAAACTgg                                     >  1:4081845/1‑39 (MQ=255)
                                                                    ggTGTCTAAAGTTGACGAGTCGCTTGAAAAACTGGCTGACGAAGTTGATGAGAGCGTGAAAGAAGCGgag  <  1:4087167/70‑1 (MQ=255)
                                                                    |                                                                     
CTTTCCGACCGCATCGATGAGTGGATGATGAACTATCTGACTGAGTTCGACGGTAAACCGTTCCAGTCGGTGTCTAAAGTTGACGAGTCGCTTGAAAAACTGGCTGACGAAGTTGATGAGAGCGCGAAAGAAGCGGAG  >  Exported/324966‑325103

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: