Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	132,226	G→T	22.6%	A173A (GCG→GCT)	yadD →	predicted transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	132,226	0	G	T	22.6%	222.1 / 41.2	97	A173A (GCG→GCT)	yadD	predicted transposase
Reads supporting (aligned to +/- strand):  ref base G (32/43);  new base T (14/8);  total (46/51)
Fisher's exact test for biased strand distribution p-value = 9.50e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACT  >  Exported/132157‑132288                                                                      |                                                               cAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGa                                                               <  1:1986915/71‑1 (MQ=255)      cTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATtctc                                                          <  1:1800412/71‑1 (MQ=255)       tttCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATtctc                                                          >  1:648428/1‑70 (MQ=255)        ttCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCTACATCGGCGGATTGCGATTCTCg                                                         >  1:2903375/1‑70 (MQ=255)         tCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGaa                                                       >  1:5084483/1‑71 (MQ=255)          cccGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCg                                                         <  1:4883306/68‑1 (MQ=255)           ccGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAac                                                      <  1:4287679/70‑1 (MQ=255)            cGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAac                                                      <  1:3269773/69‑1 (MQ=255)              cTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAacta                                                    >  1:5320433/1‑69 (MQ=255)              cTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAacta                                                    >  1:1291540/1‑69 (MQ=255)                 gtggATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCa                                               <  1:5028866/71‑1 (MQ=255)                   ggATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaa                                             <  1:879856/71‑1 (MQ=255)                    gATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaaa                                            <  1:3186170/71‑1 (MQ=255)                    gATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaaa                                            <  1:2104096/71‑1 (MQ=255)                    gATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaa                                             <  1:5527922/70‑1 (MQ=255)                     atatCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaaa                                            <  1:2659004/70‑1 (MQ=255)                        taaCCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAacta                                                    >  1:3366443/3‑59 (MQ=255)                        tCACCATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCa                                         >  1:4331714/1‑70 (MQ=255)                        tCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAacta                                                    >  1:1715133/1‑59 (MQ=255)                        tCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAacta                                                    >  1:5194850/1‑59 (MQ=255)                        tCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAacta                                                    >  1:3654363/1‑59 (MQ=255)                         cACCATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCata                                       >  1:4456591/1‑71 (MQ=255)                         cACCATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCat                                        >  1:5139991/1‑70 (MQ=255)                         cACCATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCat                                        >  1:3151006/1‑70 (MQ=255)                         cACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACata                                       >  1:137935/1‑71 (MQ=255)                          aCCATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCata                                       <  1:1682564/70‑1 (MQ=255)                          aCCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACatat                                      <  1:5411175/71‑1 (MQ=255)                             aTCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCg                                   <  1:4109234/71‑1 (MQ=255)                              tCACAGCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCg                                                         <  1:793773/48‑1 (MQ=255)                              tCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAacta                                                    <  1:1364574/53‑1 (MQ=255)                              tCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGc                                  <  1:1990572/71‑1 (MQ=255)                              tCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGc                                  <  1:3733406/71‑1 (MQ=255)                              tCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGc                                  >  1:1724271/1‑71 (MQ=255)                              tCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGc                                  <  1:2811198/71‑1 (MQ=255)                              tCACACCGGATGACGAAATCATGCAACATCGGAGGATTGCGATTCTCg                                                         <  1:3171776/48‑1 (MQ=255)                               cacaCCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGaa                                                       >  1:1119891/1‑49 (MQ=255)                               cacaCCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGaa                                                       >  1:1469273/1‑49 (MQ=255)                               cacaCCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGaa                                                       >  1:283599/1‑49 (MQ=255)                               cacaCCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGaa                                                       >  1:2427586/1‑49 (MQ=255)                               cacaCCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGa                                                        >  1:824333/1‑48 (MQ=255)                                acacCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGcc                                 <  1:5225852/70‑1 (MQ=255)                                acacCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGcc                                 <  1:1456262/70‑1 (MQ=255)                                  acCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaaa                                            <  1:4922131/57‑1 (MQ=255)                                  acCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaaa                                            <  1:1591358/57‑1 (MQ=255)                                    cGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAgcgc                            <  1:1982600/71‑1 (MQ=255)                                    cGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAgcgc                            >  1:5603944/1‑71 (MQ=255)                                    cGGATGACGAAATCATGCAACATAGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAgcg                             >  1:4314735/1‑70 (MQ=255)                                     ggATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACa                                         <  1:5499548/57‑1 (MQ=255)                                     ggATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACa                                         <  1:4411448/57‑1 (MQ=255)                                       aTGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaat                                            <  1:1765041/52‑2 (MQ=255)                                        tGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAac                                                      <  1:5469016/41‑1 (MQ=255)                                        tGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAac                                                      <  1:1419189/41‑1 (MQ=255)                                        tGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAac                                                      <  1:2405323/41‑1 (MQ=255)                                        tGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAac                                                      <  1:3950972/41‑1 (MQ=255)                                        tGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGc                                  <  1:5480886/61‑1 (MQ=255)                                        tGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACt                        <  1:5105414/71‑1 (MQ=255)                                          aCGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACtt                       >  1:4311932/1‑70 (MQ=255)                                             agaTCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGCGACCGCGATTTAAtc                   >  1:5264174/3‑70 (MQ=255)                                              aaTCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGt                  <  1:4346016/71‑1 (MQ=255)                                               aTCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGCGACcgcg                           <  1:1981471/61‑1 (MQ=255)                                               aTCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGCGACCGCGATTTAAtcgg                 <  1:3144933/71‑4 (MQ=255)                                                tCATGCAACATCGGCGTATAGCTCTGCTTGAACTGATTCAAAAGCATATTCGCGACCGCGat                         <  1:437409/62‑2 (MQ=255)                                                tCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGCGACCGCGat                         <  1:3389478/62‑2 (MQ=255)                                                tCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGCGACCGCGat                         <  1:5381492/62‑2 (MQ=255)                                                tCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGCGACCGCGAttt                       <  1:4614380/64‑1 (MQ=255)                                                tCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGCGACCGCGATTTAAtcgg                 <  1:4086207/70‑4 (MQ=255)                                                tCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaaaa                                           <  1:3964826/44‑1 (MQ=255)                                                tCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaaaa                                           <  1:2386059/44‑1 (MQ=255)                                                tCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaaaa                                           <  1:5304759/44‑1 (MQ=255)                                                 cATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCata                                       >  1:1096838/1‑47 (MQ=255)                                                 cATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCat                                        >  1:3643736/1‑46 (MQ=255)                                                 cATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCat                                        >  1:3007904/1‑46 (MQ=255)                                                 cATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCat                                        >  1:3949494/1‑46 (MQ=255)                                                 cATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGCGACCGCGat                         >  1:2371657/1‑60 (MQ=255)                                                 cATGCAACATAGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGCGACCGCGat                         >  1:3248330/1‑60 (MQ=255)                                                  aTGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACa                                         >  1:176267/1‑44 (MQ=255)                                                   tGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaa                                             <  1:202621/39‑1 (MQ=255)                                                    gCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGcga                                >  1:2947347/1‑49 (MQ=255)                                                     cAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAgcg                             >  1:1678405/1‑53 (MQ=255)                                                     cAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCt           <  1:4014833/71‑1 (MQ=255)                                                     cAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCt           >  1:121453/1‑71 (MQ=255)                                                     cAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCt           >  1:1755295/1‑71 (MQ=255)                                                      aaCATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGCGACCGCGATTTa                      >  1:2398642/1‑59 (MQ=255)                                                      aaCATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGCGACCGCGATTTa                      >  1:2550432/1‑59 (MQ=255)                                                       aCATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAgcgc                            <  1:825848/52‑1 (MQ=255)                                                         aTCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTaa                     >  1:5389881/1‑57 (MQ=255)                                                         aTCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTaa                     >  1:4756399/1‑57 (MQ=255)                                                         aTCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAg       >  1:3152257/1‑71 (MQ=255)                                                           cggcggATTGCGATTCTCGAACTACTGCAAAAACatat                                      >  1:2487730/1‑38 (MQ=255)                                                           cggcggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAAt                    >  1:4733468/1‑56 (MQ=255)                                                           cggcggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAAt                    >  1:5388259/1‑56 (MQ=255)                                                           cggcggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAAt                    >  1:4727016/1‑56 (MQ=255)                                                            ggcggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCaa    >  1:549698/1‑71 (MQ=255)                                                            ggcggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCaa    >  1:5025523/1‑71 (MQ=255)                                                            ggcggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCaa    <  1:1932043/71‑1 (MQ=255)                                                            ggcggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCaa    <  1:1726524/71‑1 (MQ=255)                                                               ggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACt  <  1:1854585/70‑1 (MQ=255)                                                                      gATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCtt          <  1:1957148/55‑1 (MQ=255)                                                                      gATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCtt          <  1:1875035/55‑1 (MQ=255)                                                                      |                                                               CAGTCCTTTCCCGCTGGTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACT  >  Exported/132157‑132288

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑