Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 385,078 T→C 18.6% I96I (ATT→ATC cstA → carbon starvation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported385,0780TC18.6% 141.7 / 26.1 70I96I (ATT→ATCcstAcarbon starvation protein
Reads supporting (aligned to +/- strand):  ref base T (19/38);  new base C (5/8);  total (24/46)
Fisher's exact test for biased strand distribution p-value = 7.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.27e-01

GCCATAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATC  >  Exported/385008‑385144
                                                                      |                                                                  
gcCATAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCAtt                                                                    <  1:2971558/71‑1 (MQ=255)
  cATAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGc                                                                  <  1:525246/71‑1 (MQ=255)
  cATAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGc                                                                  <  1:3267131/71‑1 (MQ=255)
  cATAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGc                                                                  <  1:1894336/71‑1 (MQ=255)
  cATAACGACGGGCTGGACTATGTGCCGAAGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGc                                                                  <  1:4955664/71‑1 (MQ=255)
   aTAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGcc                                                                 <  1:1730019/71‑1 (MQ=255)
    tAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCAtt                                                                    <  1:967651/67‑1 (MQ=255)
    tAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCAtt                                                                    <  1:5663818/67‑1 (MQ=255)
    tAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCAtt                                                                    <  1:5657472/67‑1 (MQ=255)
    tAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCAtt                                                                    <  1:5153350/67‑1 (MQ=255)
    tAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCg                                                                <  1:2051506/71‑1 (MQ=255)
      acgacgGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCggc                                                              >  1:1702531/1‑70 (MQ=255)
      acgacgGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCgg                                                               <  1:1060206/70‑1 (MQ=255)
      acgacgGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCgg                                                               <  1:781360/70‑1 (MQ=255)
      acgacgGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATATTGCGGCCATTGCCg                                                                >  1:370272/1‑69 (MQ=255)
       cgacgGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAg                                                             <  1:1969869/71‑1 (MQ=255)
       cgacgGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAg                                                             >  1:3004488/1‑71 (MQ=255)
       cgacgGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAg                                                             <  1:2937430/71‑1 (MQ=255)
       cgacgGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAg                                                             <  1:2894153/71‑1 (MQ=255)
       cgacgGACTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAg                                                             >  1:739857/1‑71 (MQ=255)
        gacgGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGc                                                            <  1:2977286/71‑1 (MQ=255)
         acgGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAg                                                             <  1:1323330/69‑1 (MQ=255)
          cgGGCTGGACTATGTGCCGACGGACAGGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAg                                                          <  1:744356/71‑1 (MQ=255)
          cgGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAg                                                          <  1:5272378/71‑1 (MQ=255)
          cgGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAg                                                          <  1:5164516/71‑1 (MQ=255)
           gggCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAgg                                                         <  1:19354/71‑1 (MQ=255)
           gggCTGGACTATGTGCAGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAgg                                                         <  1:2840582/71‑1 (MQ=255)
              cTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTcc                                                      <  1:3776807/71‑1 (MQ=255)
                  aCTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGa                                                              <  1:3179177/59‑1 (MQ=255)
                  aCTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCtg                                                  <  1:3968233/71‑1 (MQ=255)
                    tATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCtggt                                                >  1:1513531/1‑71 (MQ=255)
                      tgtgCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCtggtg                                               <  1:2850864/70‑1 (MQ=255)
                          ccGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGcc                                          >  1:489017/1‑71 (MQ=255)
                           cGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCg                                         >  1:4572003/1‑71 (MQ=255)
                            gACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCgg                                        <  1:554019/71‑1 (MQ=255)
                            gACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCgg                                        <  1:1661392/71‑1 (MQ=255)
                              ctgACAAGAAAGTGCTGTTCGGGCACCATTTTGCGGCCATTGCCGGAGCAGGGCCGCTGGTGGGGcc                                          <  1:3712220/65‑1 (MQ=255)
                               ggACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCtg                                                  >  1:2584290/1‑58 (MQ=255)
                               ggACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTAc                                     >  1:1046367/1‑71 (MQ=255)
                                  cAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTgg                                  <  1:2712877/71‑1 (MQ=255)
                                   aaGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTggc                                 >  1:3787673/1‑71 (MQ=255)
                                         gtgtTGTTTGGTCACCACTTCGCCGCTATCGCCGGTGCTGGTCCGCtggt                                                <  1:1850922/50‑1 (MQ=255)
                                         gtgtTGTTTGGTCACCACTTCGCCGCTATCGCCGGTGCTGGTCCGCtggt                                                <  1:2651637/50‑1 (MQ=255)
                                         gTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCa                           <  1:1296041/71‑1 (MQ=255)
                                         gTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCa                           <  1:1289279/71‑1 (MQ=255)
                                          tGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCg                                                     <  1:33002/44‑1 (MQ=255)
                                          tGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCg                                                     <  1:4700197/44‑1 (MQ=255)
                                           gttGTTTGGTCACCACTTCGCCGCTATCGCCGGTGCTGGTCCGCTGGTGGGTCCGGTTCTCgccgcg                             <  1:1649463/65‑1 (MQ=255)
                                            ttGTTTGGTCACCACTTCGCCGCTATCGCCGGTGCTGGTCCGCtggt                                                >  1:2677279/2‑47 (MQ=255)
                                            ttGTTTGGTCACCACTTCGCCGCTATCGCCGGTGCTGGTCCGCtggt                                                >  1:4289989/2‑47 (MQ=255)
                                            cTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAAt                        >  1:868934/1‑71 (MQ=255)
                                             tGTTTGGTCACCACTTCGCCGCTATCGCCGGTGCTGGTCCGCTGGTGGGTCCGGTTCTCGCCGCGCa                           >  1:3884430/1‑67 (MQ=255)
                                             tGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAAt                        <  1:5489501/70‑1 (MQ=255)
                                              gTTTGGTCACCACTTCGCCGCTATCGCCGGTGCTGGTCCGCTGGTGGGTCCGGTTCTCGCCGCGCAGATg                       <  1:5279289/70‑1 (MQ=255)
                                              gTTTGGTCACCACTTCGCCGCTATCGCCGGTGCTGGTCCGCTGGTGGGTCCGGTTCTCGCCGCGCAGATg                       <  1:5612341/70‑1 (MQ=255)
                                                ttggTCACCACTTCGCCGCTATCGCCGGTGCTGGTCCGCTGGTGGGTCCGGTTCTCGCCGCGCAGATGGGc                    >  1:230749/3‑71 (MQ=255)
                                                 cGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGc                    >  1:2273881/1‑70 (MQ=255)
                                                  ggTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCt                   <  1:1631204/70‑1 (MQ=255)
                                                   gTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGc                    >  1:204562/1‑68 (MQ=255)
                                                     cACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGt                                       <  1:625748/47‑1 (MQ=255)
                                                     cACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGt                                       <  1:1850941/47‑1 (MQ=255)
                                                      aCCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTg              >  1:4949266/1‑71 (MQ=255)
                                                      aCCACTTCGCCGCTATCGCCGGTGCTGGTCCGCTGGTGGGTCCGGTTCTCGCCGCGCAGATGGGCTACCt               <  1:3629173/70‑1 (MQ=255)
                                                       ccACTTCGCCGCTATCGCCGGTGCTGGTCCGCTGGTGGGTCCGGTTCTCGCCGCGCAGATGGGCt                   <  1:273080/65‑1 (MQ=255)
                                                       ccACTTCGCCGCTATCGCCGGTGCTGGTCCGCTGGTGGGTCCGGTTCTCGCCGCGCAGATGGGCt                   <  1:4808523/65‑1 (MQ=255)
                                                       ccACTTCGCCGCTATCGCCGGTGCTGGTCCGCTGGTGGGTCCGGTTCTCGCCGCGCAGATGGGCTACCTGc             >  1:3509648/1‑71 (MQ=255)
                                                          ttttGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCt                   <  1:647690/62‑1 (MQ=255)
                                                          ttttGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCgg          >  1:5385301/1‑71 (MQ=255)
                                                          ttttGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCgg          >  1:373704/1‑71 (MQ=255)
                                                            ttGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCggg         >  1:2082745/1‑70 (MQ=255)
                                                            ttGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCggg         >  1:283420/1‑70 (MQ=255)
                                                                  ccATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATc  >  1:724685/1‑71 (MQ=255)
                                                                   cATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACt                                    <  1:4810106/36‑1 (MQ=255)
                                                                    aTTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACt                                    <  1:1446467/35‑1 (MQ=255)
                                                                    aTTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACt                                    <  1:3462893/35‑1 (MQ=255)
                                                                      |                                                                  
GCCATAACGACGGGCTGGACTATGTGCCGACGGACAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATC  >  Exported/385008‑385144

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: