Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 385,111 G→C 36.2% L107L (CTG→CTC cstA → carbon starvation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported385,1110GC36.2% 40.6 / 42.8 47L107L (CTG→CTCcstAcarbon starvation protein
Reads supporting (aligned to +/- strand):  ref base G (15/15);  new base C (7/10);  total (22/25)
Fisher's exact test for biased strand distribution p-value = 7.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCG  >  Exported/385042‑385177
                                                                     |                                                                  
cAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTgg                                                                   <  1:2712877/71‑1 (MQ=255)
 aaGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTggc                                                                  >  1:3787673/1‑71 (MQ=255)
       gTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCa                                                            <  1:1296041/71‑1 (MQ=255)
       gTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCa                                                            <  1:1289279/71‑1 (MQ=255)
         gttGTTTGGTCACCACTTCGCCGCTATCGCCGGTGCTGGTCCGCTGGTGGGTCCGGTTCTCgccgcg                                                              <  1:1649463/65‑1 (MQ=255)
          cTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAAt                                                         >  1:868934/1‑71 (MQ=255)
           tGTTTGGTCACCACTTCGCCGCTATCGCCGGTGCTGGTCCGCTGGTGGGTCCGGTTCTCGCCGCGCa                                                            >  1:3884430/1‑67 (MQ=255)
           tGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAAt                                                         <  1:5489501/70‑1 (MQ=255)
            gTTTGGTCACCACTTCGCCGCTATCGCCGGTGCTGGTCCGCTGGTGGGTCCGGTTCTCGCCGCGCAGATg                                                        <  1:5279289/70‑1 (MQ=255)
            gTTTGGTCACCACTTCGCCGCTATCGCCGGTGCTGGTCCGCTGGTGGGTCCGGTTCTCGCCGCGCAGATg                                                        <  1:5612341/70‑1 (MQ=255)
              ttggTCACCACTTCGCCGCTATCGCCGGTGCTGGTCCGCTGGTGGGTCCGGTTCTCGCCGCGCAGATGGGc                                                     >  1:230749/3‑71 (MQ=255)
               cGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGc                                                     >  1:2273881/1‑70 (MQ=255)
                ggTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCt                                                    <  1:1631204/70‑1 (MQ=255)
                 gTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGc                                                     >  1:204562/1‑68 (MQ=255)
                    aCCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTg                                               >  1:4949266/1‑71 (MQ=255)
                    aCCACTTCGCCGCTATCGCCGGTGCTGGTCCGCTGGTGGGTCCGGTTCTCGCCGCGCAGATGGGCTACCt                                                <  1:3629173/70‑1 (MQ=255)
                     ccACTTCGCCGCTATCGCCGGTGCTGGTCCGCTGGTGGGTCCGGTTCTCGCCGCGCAGATGGGCt                                                    <  1:273080/65‑1 (MQ=255)
                     ccACTTCGCCGCTATCGCCGGTGCTGGTCCGCTGGTGGGTCCGGTTCTCGCCGCGCAGATGGGCt                                                    <  1:4808523/65‑1 (MQ=255)
                     ccACTTCGCCGCTATCGCCGGTGCTGGTCCGCTGGTGGGTCCGGTTCTCGCCGCGCAGATGGGCTACCTGc                                              >  1:3509648/1‑71 (MQ=255)
                        ttttGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCt                                                    <  1:647690/62‑1 (MQ=255)
                        ttttGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCgg                                           >  1:5385301/1‑71 (MQ=255)
                        ttttGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCgg                                           >  1:373704/1‑71 (MQ=255)
                          ttGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCggg                                          >  1:283420/1‑70 (MQ=255)
                          ttGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCggg                                          >  1:2082745/1‑70 (MQ=255)
                                ccATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATc                                   >  1:724685/1‑71 (MQ=255)
                                     gCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTa                                                   >  1:2431413/1‑50 (MQ=255)
                                      ccGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGgctg                             <  1:3752196/71‑1 (MQ=255)
                                       cGGTGCTGGTCCGCTGGTGGGTCCGGTTCTCGCCGTGCAGATGGGCTACCTGCCTGGCACGCTGTGgctgc                            <  1:2802760/71‑1 (MQ=255)
                                           gCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGgatgat                                    >  1:2519884/1‑59 (MQ=255)
                                           gCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGgatgat                                    >  1:3264642/1‑59 (MQ=255)
                                            cAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCt                       <  1:1548247/71‑1 (MQ=255)
                                              ggTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCgggg                                         <  1:455446/51‑1 (MQ=255)
                                                tCCGCTGGTGGGTCCGGTTCTCGCCGCGCAGATGGGCTACCTGCCTGGCACGCTGTGGCTGCTGGcg                       >  1:4242236/1‑66 (MQ=255)
                                                  cGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTAGCTGGggt                  <  1:4945212/70‑1 (MQ=255)
                                                   gCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGggtgg                <  1:2738382/71‑1 (MQ=255)
                                                    cTGGTGGGTCCGGTTCTCGCCGCGCAGATGGGCTACCTGCCTGGCACGCTGTGGCTGCTGGCGGGggtcgt               <  1:3437747/71‑1 (MQ=255)
                                                       gtggGTCCGGTTCTCGCCGCGCAGATGGGCTACCTGCCTGGCACGCTGTGgct                              <  1:499600/53‑1 (MQ=255)
                                                       gtggGTCCGGTTCTCGCCGCGCAGATGGGCTACCTGCCTGGCACGCTGTGGCTGCTGGCGGGGGTCGTgct            >  1:449019/1‑71 (MQ=255)
                                                       gtggGTCCGGTTCTCGCCGCGCAGATGGGCTACCTGCCTGGCACGCTGTGGCTGCTGGCGGGGGTCGTgct            >  1:3439234/1‑71 (MQ=255)
                                                       gtggGTCCGGTTCGCGCCGCGCAGATGGGCTACCTGCCTGGCACGCTGTGgct                              <  1:3781756/53‑1 (MQ=255)
                                                          ggtccGGTTCTCGCCGCGCAGATGGGCTACCTGCCTGGCACGCTGTGGCTGCTGGCGGGGGTCGTGCTGGc         >  1:3274463/4‑71 (MQ=255)
                                                           ggCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGcc        <  1:5507184/71‑1 (MQ=255)
                                                                gTACTGGCGGCGCAAATGGGCTACCTGCCGGGgatg                                      <  1:1668014/36‑1 (MQ=255)
                                                                gTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCg                         <  1:1648172/49‑1 (MQ=255)
                                                                 tACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGggtgg                <  1:5656408/57‑1 (MQ=255)
                                                                 tACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGCCTGCTCGCTGGggtgg                <  1:3690331/57‑1 (MQ=255)
                                                                  aCTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTgg                     >  1:4122070/1‑51 (MQ=255)
                                                                  aCTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCcggtgcg  >  1:4901219/1‑70 (MQ=255)
                                                                   cTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTtctc           >  1:2271265/1‑60 (MQ=255)
                                                                     ggcggcGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTtctc           >  1:3012489/1‑58 (MQ=255)
                                                                     |                                                                  
CAAGAAAGTGCTGTTCGGTCACCATTTTGCGGCCATTGCCGGAGCAGGTCCGCTGGTGGGGCCGGTACTGGCGGCGCAAATGGGCTACCTGCCGGGGATGATCTGGCTGCTCGCTGGGGTGGTTCTCGCCGGTGCG  >  Exported/385042‑385177

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: