Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 385,429 G→T 12.8% L213L (CTG→CTT) ‡ cstA → carbon starvation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported385,4290GT12.8% 184.7 / 9.6 62L213L (CTG→CTT) ‡cstAcarbon starvation protein
Reads supporting (aligned to +/- strand):  ref base G (29/25);  new base T (3/5);  total (32/30)
Fisher's exact test for biased strand distribution p-value = 4.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.87e-01

GTGGGGAACATATACCGTTGCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCCATTATCTCTGG  >  Exported/385360‑385497
                                                                     |                                                                    
gTGGGGAACATATACCGTTGCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGc                                                                     >  1:4831295/1‑71 (MQ=255)
 tGGGGAACATATACCGTTGCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCg                                                                    >  1:2575199/1‑71 (MQ=255)
     gAACATATACCGTTGCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTACATGCGCTATCTGCGTCCg                                                                <  1:1323712/71‑1 (MQ=255)
       aCATATACCGTTGCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCggg                                                              <  1:951749/71‑1 (MQ=255)
          tataCCGTTGCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCg                                                           <  1:2811345/71‑1 (MQ=255)
          tataCCGTTGCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCg                                                           >  1:2122117/1‑71 (MQ=255)
           ataCCGTTGCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCg                                                           >  1:59461/1‑70 (MQ=255)
            taCCGTTGCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTa                                                         >  1:3992845/1‑71 (MQ=255)
              ccGTTGCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTAtt                                                       <  1:865078/71‑1 (MQ=255)
              ccGTTGCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTAtt                                                       >  1:371778/1‑71 (MQ=255)
              ccGTTGCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTAtt                                                       <  1:4153633/71‑1 (MQ=255)
                 ttGCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGc                                                    <  1:3186701/71‑1 (MQ=255)
                    cGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGa                                                  <  1:3754821/70‑1 (MQ=255)
                      ttCACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGt                                                          <  1:1014246/60‑1 (MQ=255)
                        cACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAgtgt                                             >  1:107078/1‑71 (MQ=255)
                        cACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAgtgt                                             >  1:4635427/1‑71 (MQ=255)
                        cACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAgtg                                              <  1:1659745/70‑1 (MQ=255)
                        cACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAgtg                                              >  1:5071817/1‑70 (MQ=255)
                         aCCATTCCGCTGGCGCTGTTTATTGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTc                                            >  1:4454511/1‑71 (MQ=255)
                         aCCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTc                                                                  >  1:295969/1‑49 (MQ=255)
                             ttCCGCTGTCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAgtgg                                             <  1:163748/66‑2 (MQ=255)
                             ttCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCg                                                   <  1:2667754/60‑1 (MQ=255)
                             ttCCGCTGGCGCTGTTTATGGGGATCTACCCGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCg                                           <  1:3361179/68‑1 (MQ=255)
                              tCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCa                                       >  1:4031201/1‑71 (MQ=255)
                                    tgcgcTGTTTATGGGTATCTACATGCGCTTTATTCGTCCGGGGCGTGTGGGTGAAGTCTCTGTc                                        >  1:4899344/2‑64 (MQ=255)
                                    tgcgcTGTTTATGGGTATCTACATGCGCTTTATTCGTCCGGGGCGTGTGGGTGAAGTCTCTGTc                                        >  1:4975636/2‑64 (MQ=255)
                                     gcgcTGTTTATGGGTATCTACATGCGCTTTATTCGTCCGGGGCGTGTGGGTGAAGTCTCTGTCATTGGta                                 <  1:554944/70‑2 (MQ=255)
                                     gcgcTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTc                                 <  1:3730904/70‑1 (MQ=255)
                                      cgcTGTTTATGGGTATCTACATGCGCTTTATTCGTCCGGGGCGTGTGGGTGAAGTCTCTGTCATTGGtatc                               <  1:2715536/71‑4 (MQ=255)
                                      cgcTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCgggg                                                             >  1:1152620/1‑41 (MQ=255)
                                       gcTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTgg                              <  1:2204072/71‑1 (MQ=255)
                                        cTGTTTATGGGTATCTACATGCGCTTTATTCGTCCGGGGCGTGTGGGTGAAgtct                                             <  1:4865738/55‑3 (MQ=255)
                                          gTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTgg                              <  1:4519854/68‑1 (MQ=255)
                                          gTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTAt                           >  1:814768/1‑71 (MQ=255)
                                          gTTTATGGGGATCTACATGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTAt                           >  1:1186824/1‑71 (MQ=255)
                                           tttATGGGTATCTACATGCGCTTTATTCGTCCGGGGCGt                                                          <  1:2453367/39‑1 (MQ=19)
                                           tttATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTAtt                          <  1:914747/71‑1 (MQ=255)
                                            ttATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTa                            >  1:671109/1‑68 (MQ=255)
                                             tATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATc                                     >  1:2601464/1‑58 (MQ=255)
                                               tGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCAt                                      >  1:2479440/1‑55 (MQ=255)
                                               tGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTg                      <  1:2598206/71‑1 (MQ=255)
                                                 gggATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAg                                                >  1:5550889/1‑43 (MQ=255)
                                                 gggATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAg                                                >  1:956833/1‑43 (MQ=255)
                                                 gggATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAg                                                >  1:4471752/1‑43 (MQ=255)
                                                   gATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGAttt                  <  1:1930464/71‑1 (MQ=255)
                                                    aTCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAgtg                                              <  1:1589641/42‑1 (MQ=255)
                                                    aTCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTg                      <  1:1189991/66‑1 (MQ=255)
                                                    aTCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTg                      <  1:1950871/66‑1 (MQ=255)
                                                     tCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGAtttt                 >  1:477107/1‑70 (MQ=255)
                                                     tCTACATGCGCTTTATTCGTCCGGGGCGTGTGGGTGaa                                                 >  1:862661/1‑38 (MQ=255)
                                                      cTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTc                                            >  1:353490/1‑42 (MQ=255)
                                                      cTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTc                                            >  1:3118638/1‑42 (MQ=255)
                                                       tACATGCGCTTTATTCGTCCGGGGCGTGTGGGTGAAGtctct                                           <  1:2387922/42‑2 (MQ=255)
                                                        aCCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTa                            >  1:2881672/1‑56 (MQ=255)
                                                          cTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCCAt           <  1:661134/71‑1 (MQ=255)
                                                            gcgcTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCCATTa         >  1:1937530/1‑71 (MQ=255)
                                                                  tCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCAt                                      >  1:321450/1‑36 (MQ=255)
                                                                  tCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGAtt                   <  1:3591105/55‑1 (MQ=255)
                                                                  tCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCCATTATctct    <  1:32309/70‑1 (MQ=255)
                                                                  tCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCCATTATctct    <  1:3178753/70‑1 (MQ=255)
                                                                   cTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCCATTATctct    >  1:1951846/1‑69 (MQ=255)
                                                                   cTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCCATTATCTCTgg  >  1:815464/1‑71 (MQ=255)
                                                                     |                                                                    
GTGGGGAACATATACCGTTGCGTTCACCATTCCGCTGGCGCTGTTTATGGGGATCTACCTGCGCTATCTGCGTCCGGGGCGTATTGGCGAAGTGTCGGTCATCGGTCTGGTATTCCTGATTTTCGCCATTATCTCTGG  >  Exported/385360‑385497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: