Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 385,610 T→C 10.0% L274L (TTA→CTA) ‡ cstA → carbon starvation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported385,6100TC10.0% 157.0 / 7.6 60L274L (TTA→CTA) ‡cstAcarbon starvation protein
Reads supporting (aligned to +/- strand):  ref base T (28/26);  new base C (2/4);  total (30/30)
Fisher's exact test for biased strand distribution p-value = 6.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01

CTTTACCGGCGTGCAGTTGACCTGGATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGG  >  Exported/385540‑385680
                                                                      |                                                                      
cTTTACCGGCGTGCAGTTGACCTGGATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGt                                                                        <  1:4412872/71‑1 (MQ=255)
  ttACCGGCGTGCAGTTGACCTGGATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTa                                                                      >  1:4822375/1‑71 (MQ=255)
    aCCGGCGTGCAGTTGACCTGGATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTAct                                                                    >  1:196598/1‑71 (MQ=255)
        gCGTGCAGTTGACCTGGATGCTGGTGGGTTACTGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTActgctg                                                                >  1:3156345/1‑71 (MQ=255)
        gCGTGCAGTTGACCTGGATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTActgctg                                                                >  1:3949890/1‑71 (MQ=255)
             cAGTTGACCTGGATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGccc                                                            <  1:1764602/70‑1 (MQ=255)
              aGTTGACCTGGATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCcg                                                          <  1:3206180/71‑1 (MQ=255)
                ttGACCTGGATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGATGGCCCcccg                                                        >  1:852744/1‑71 (MQ=255)
                  gACCTGGATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGt                                                       >  1:5671959/1‑70 (MQ=255)
                  gACCTGGATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGt                                                       >  1:1315308/1‑70 (MQ=255)
                    ccTGGATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGAc                                                    >  1:5090822/1‑71 (MQ=255)
                     cTGGATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGt                                                                        <  1:3908492/50‑1 (MQ=255)
                     cTGGATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTAc                                                                     <  1:311343/53‑1 (MQ=255)
                     cTGGATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTAc                                                                     <  1:1617529/53‑1 (MQ=255)
                     cTGGATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTAc                                                                     <  1:851523/53‑1 (MQ=255)
                      tGGATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGt                                                                        <  1:3426460/49‑1 (MQ=255)
                       ggATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTa                                                                      >  1:759079/1‑50 (MQ=255)
                       ggATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTa                                                                      >  1:573667/1‑50 (MQ=255)
                       ggATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTAc                                                 >  1:4665770/1‑71 (MQ=255)
                       ggATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCACCGCGTg                                                      >  1:5331024/1‑66 (MQ=255)
                        gATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGcccc                                                           >  1:4819517/1‑60 (MQ=255)
                        gATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTAcc                                                >  1:1069388/1‑71 (MQ=255)
                        gATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTAcc                                                >  1:4752566/1‑71 (MQ=255)
                        gATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTAc                                                 <  1:4483431/70‑1 (MQ=255)
                         aTGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACct                                               >  1:4723507/1‑71 (MQ=255)
                              ggtggGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTa                                          >  1:4706999/1‑71 (MQ=255)
                              ggtggGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTa                                          >  1:144673/1‑71 (MQ=255)
                               gtggGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTAc                                         >  1:2173621/1‑71 (MQ=255)
                                tggGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGt                                                                        <  1:2747739/39‑1 (MQ=255)
                                tggGTTACGGTTTTGTGGCAGCGGTGCTGCCGGTGTGGTTActgctg                                                                <  1:2753046/47‑1 (MQ=255)
                                   gTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTAct                                                                    <  1:1890492/40‑1 (MQ=255)
                                   gTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTc                                     <  1:3594466/71‑1 (MQ=255)
                                     tACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCt                                   >  1:556715/1‑71 (MQ=255)
                                     tACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCt                                   >  1:4062551/1‑71 (MQ=255)
                                      aCGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTAcc                                                >  1:4165621/1‑57 (MQ=255)
                                        ggTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGaa                                <  1:833257/71‑1 (MQ=255)
                                         gTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGaaa                               <  1:1130967/71‑1 (MQ=255)
                                            ttGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTcc                                    <  1:2931034/63‑1 (MQ=255)
                                            ttGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATc                            >  1:3968050/1‑71 (MQ=255)
                                                    gcggTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATc                            <  1:2092252/63‑1 (MQ=255)
                                                    gcggTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATc                            <  1:5671157/63‑1 (MQ=255)
                                                    gcggTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATc                            <  1:5542898/63‑1 (MQ=255)
                                                    gcggTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATc                            <  1:3265248/63‑1 (MQ=255)
                                                     cactgctgcCAGTGTGGCTGATCCTCGCACCGCGCGACTATCTGGCAACCTTCCTGaaaa                              >  1:907676/4‑60 (MQ=255)
                                                        tgctgcCAGTGTGGCTGATCCTCGCACCGCGCGACTATCTGGCAACCTTCCTGa                                 <  1:3358470/54‑1 (MQ=255)
                                                         gctgcCAGTGTGGCTGATCCTCGCACCGCGCGACTATCTGGCAACCTTCCTGAAAATCGGCGTTAtcgtcg               >  1:3116366/1‑71 (MQ=255)
                                                          ctgcCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTa                     >  1:1879722/1‑64 (MQ=255)
                                                          ctgcCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCgg              <  1:4568831/71‑1 (MQ=255)
                                                          ctgcCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCgg              >  1:1053986/1‑71 (MQ=255)
                                                          ctgcCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCgg              >  1:3163480/1‑71 (MQ=255)
                                                          ctgcCAGTGTGGCTGATCCTCGCACCGCGCGACTATCTGGCAACCTTCCTGAAAATCGGCGTTAtcgtcg               <  1:5292467/70‑1 (MQ=255)
                                                          ctgcCAGTGTGGCTGATCCTCGCACCGCGCGACTATCTGGCAACCTTCCTGAAAATCGGCGTTAtcgtc                <  1:5149104/69‑1 (MQ=255)
                                                          ctgcCAGTGTGGCTGATCCTCGCACCGCGCGACTATCTGGCAACCTTCCTGAAAATCGGCGTTAtcgtc                <  1:1211863/69‑1 (MQ=255)
                                                             ccGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGa                                 <  1:1612282/49‑1 (MQ=255)
                                                             ccGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCgg              <  1:5232363/68‑1 (MQ=255)
                                                             ccGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCgg              <  1:1553514/68‑1 (MQ=255)
                                                               ggTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTg          <  1:1446727/70‑1 (MQ=255)
                                                                 tgtgGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGc        <  1:5483196/70‑1 (MQ=255)
                                                                 tgtgGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCg       <  1:457091/71‑1 (MQ=255)
                                                                  gtgGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCgg      >  1:2327305/1‑71 (MQ=255)
                                                                   tgGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGaa                                <  1:2177273/44‑1 (MQ=255)
                                                                     gTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATGGTCGGt             <  1:5998/61‑1 (MQ=255)
                                                                     gTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCTGTCTGGCGGTAg   <  1:1606371/71‑1 (MQ=255)
                                                                     gTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAg   >  1:3797468/1‑71 (MQ=255)
                                                                      ttACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAgg  >  1:2483854/1‑71 (MQ=255)
                                                                      |                                                                      
CTTTACCGGCGTGCAGTTGACCTGGATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGG  >  Exported/385540‑385680

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: