Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 385,612 A→G 10.3% L274L (TTA→TTG) ‡ cstA → carbon starvation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported385,6120AG10.3% 151.9 / 7.3 58L274L (TTA→TTG) ‡cstAcarbon starvation protein
Reads supporting (aligned to +/- strand):  ref base A (26/26);  new base G (2/4);  total (28/30)
Fisher's exact test for biased strand distribution p-value = 6.71e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

TTACCGGCGTGCAGTTGACCTGGATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGG  >  Exported/385542‑385680
                                                                      |                                                                    
ttACCGGCGTGCAGTTGACCTGGATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTa                                                                      >  1:4822375/1‑71 (MQ=255)
  aCCGGCGTGCAGTTGACCTGGATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTAct                                                                    >  1:196598/1‑71 (MQ=255)
      gCGTGCAGTTGACCTGGATGCTGGTGGGTTACTGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTActgctg                                                                >  1:3156345/1‑71 (MQ=255)
      gCGTGCAGTTGACCTGGATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTActgctg                                                                >  1:3949890/1‑71 (MQ=255)
           cAGTTGACCTGGATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGccc                                                            <  1:1764602/70‑1 (MQ=255)
            aGTTGACCTGGATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCcg                                                          <  1:3206180/71‑1 (MQ=255)
              ttGACCTGGATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGATGGCCCcccg                                                        >  1:852744/1‑71 (MQ=255)
                gACCTGGATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGt                                                       >  1:1315308/1‑70 (MQ=255)
                gACCTGGATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGt                                                       >  1:5671959/1‑70 (MQ=255)
                  ccTGGATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGAc                                                    >  1:5090822/1‑71 (MQ=255)
                   cTGGATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTAc                                                                     <  1:311343/53‑1 (MQ=255)
                   cTGGATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTAc                                                                     <  1:1617529/53‑1 (MQ=255)
                   cTGGATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTAc                                                                     <  1:851523/53‑1 (MQ=255)
                     ggATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTa                                                                      >  1:573667/1‑50 (MQ=255)
                     ggATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTa                                                                      >  1:759079/1‑50 (MQ=255)
                     ggATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTAc                                                 >  1:4665770/1‑71 (MQ=255)
                     ggATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCACCGCGTg                                                      >  1:5331024/1‑66 (MQ=255)
                      gATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGcccc                                                           >  1:4819517/1‑60 (MQ=255)
                      gATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTAcc                                                >  1:4752566/1‑71 (MQ=255)
                      gATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTAcc                                                >  1:1069388/1‑71 (MQ=255)
                      gATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTAc                                                 <  1:4483431/70‑1 (MQ=255)
                       aTGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACct                                               >  1:4723507/1‑71 (MQ=255)
                            ggtggGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTa                                          >  1:144673/1‑71 (MQ=255)
                            ggtggGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTa                                          >  1:4706999/1‑71 (MQ=255)
                             gtggGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTAc                                         >  1:2173621/1‑71 (MQ=255)
                              tggGTTACGGTTTTGTGGCAGCGGTGCTGCCGGTGTGGTTActgctg                                                                <  1:2753046/47‑1 (MQ=255)
                                 gTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTAct                                                                    <  1:1890492/40‑1 (MQ=255)
                                 gTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTc                                     <  1:3594466/71‑1 (MQ=255)
                                   tACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCt                                   >  1:4062551/1‑71 (MQ=255)
                                   tACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCt                                   >  1:556715/1‑71 (MQ=255)
                                    aCGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTAcc                                                >  1:4165621/1‑57 (MQ=255)
                                      ggTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGaa                                <  1:833257/71‑1 (MQ=255)
                                       gTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGaaa                               <  1:1130967/71‑1 (MQ=255)
                                          ttGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTcc                                    <  1:2931034/63‑1 (MQ=255)
                                          ttGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATc                            >  1:3968050/1‑71 (MQ=255)
                                                  gcggTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATc                            <  1:3265248/63‑1 (MQ=255)
                                                  gcggTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATc                            <  1:5671157/63‑1 (MQ=255)
                                                  gcggTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATc                            <  1:5542898/63‑1 (MQ=255)
                                                  gcggTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATc                            <  1:2092252/63‑1 (MQ=255)
                                                   cactgctgcCAGTGTGGCTGATCCTCGCACCGCGCGACTATCTGGCAACCTTCCTGaaaa                              >  1:907676/4‑60 (MQ=255)
                                                      tgctgcCAGTGTGGCTGATCCTCGCACCGCGCGACTATCTGGCAACCTTCCTGa                                 <  1:3358470/54‑1 (MQ=255)
                                                       gctgcCAGTGTGGCTGATCCTCGCACCGCGCGACTATCTGGCAACCTTCCTGAAAATCGGCGTTAtcgtcg               >  1:3116366/1‑71 (MQ=255)
                                                        ctgcCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTa                     >  1:1879722/1‑64 (MQ=255)
                                                        ctgcCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCgg              >  1:3163480/1‑71 (MQ=255)
                                                        ctgcCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCgg              >  1:1053986/1‑71 (MQ=255)
                                                        ctgcCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCgg              <  1:4568831/71‑1 (MQ=255)
                                                        ctgcCAGTGTGGCTGATCCTCGCACCGCGCGACTATCTGGCAACCTTCCTGAAAATCGGCGTTAtcgtcg               <  1:5292467/70‑1 (MQ=255)
                                                        ctgcCAGTGTGGCTGATCCTCGCACCGCGCGACTATCTGGCAACCTTCCTGAAAATCGGCGTTAtcgtc                <  1:1211863/69‑1 (MQ=255)
                                                        ctgcCAGTGTGGCTGATCCTCGCACCGCGCGACTATCTGGCAACCTTCCTGAAAATCGGCGTTAtcgtc                <  1:5149104/69‑1 (MQ=255)
                                                           ccGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGa                                 <  1:1612282/49‑1 (MQ=255)
                                                           ccGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCgg              <  1:5232363/68‑1 (MQ=255)
                                                           ccGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCgg              <  1:1553514/68‑1 (MQ=255)
                                                             ggTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTg          <  1:1446727/70‑1 (MQ=255)
                                                               tgtgGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGc        <  1:5483196/70‑1 (MQ=255)
                                                               tgtgGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCg       <  1:457091/71‑1 (MQ=255)
                                                                gtgGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCgg      >  1:2327305/1‑71 (MQ=255)
                                                                 tgGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGaa                                <  1:2177273/44‑1 (MQ=255)
                                                                   gTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATGGTCGGt             <  1:5998/61‑1 (MQ=255)
                                                                   gTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCTGTCTGGCGGTAg   <  1:1606371/71‑1 (MQ=255)
                                                                   gTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAg   >  1:3797468/1‑71 (MQ=255)
                                                                    ttACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAgg  >  1:2483854/1‑71 (MQ=255)
                                                                      aCTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATc                            >  1:5690135/1‑43 (MQ=255)
                                                                      aCTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGAc                       >  1:2137469/1‑48 (MQ=255)
                                                                      aCTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTg          <  1:5039293/61‑1 (MQ=255)
                                                                      |                                                                    
TTACCGGCGTGCAGTTGACCTGGATGCTGGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGG  >  Exported/385542‑385680

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: