Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 385,637 T→G 10.4% S283A (TCT→GCT) ‡ cstA → carbon starvation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported385,6370TG10.4% 130.0 / 8.8 57S283A (TCT→GCT) ‡cstAcarbon starvation protein
Reads supporting (aligned to +/- strand):  ref base T (20/31);  new base G (2/4);  total (22/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGATTATGCGCCCGACGCTGA  >  Exported/385570‑385706
                                                                   |                                                                     
ggtggGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTa                                                                    >  1:144673/1‑71 (MQ=255)
ggtggGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTa                                                                    >  1:4706999/1‑71 (MQ=255)
 gtggGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTAc                                                                   >  1:2173621/1‑71 (MQ=255)
     gTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTc                                                               <  1:3594466/71‑1 (MQ=255)
       tACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCt                                                             >  1:556715/1‑71 (MQ=255)
       tACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCt                                                             >  1:4062551/1‑71 (MQ=255)
          ggTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGaa                                                          <  1:833257/71‑1 (MQ=255)
           gTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGaaa                                                         <  1:1130967/71‑1 (MQ=255)
              ttGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTcc                                                              <  1:2931034/63‑1 (MQ=255)
              ttGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATc                                                      >  1:3968050/1‑71 (MQ=255)
                      gcggTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATc                                                      <  1:5671157/63‑1 (MQ=255)
                      gcggTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATc                                                      <  1:5542898/63‑1 (MQ=255)
                      gcggTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATc                                                      <  1:3265248/63‑1 (MQ=255)
                      gcggTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATc                                                      <  1:2092252/63‑1 (MQ=255)
                       cactgctgcCAGTGTGGCTGATCCTCGCACCGCGCGACTATCTGGCAACCTTCCTGaaaa                                                        >  1:907676/4‑60 (MQ=255)
                          tgctgcCAGTGTGGCTGATCCTCGCACCGCGCGACTATCTGGCAACCTTCCTGa                                                           <  1:3358470/54‑1 (MQ=255)
                           gctgcCAGTGTGGCTGATCCTCGCACCGCGCGACTATCTGGCAACCTTCCTGAAAATCGGCGTTAtcgtcg                                         >  1:3116366/1‑71 (MQ=255)
                            ctgcCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTa                                               >  1:1879722/1‑64 (MQ=255)
                            ctgcCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCgg                                        >  1:1053986/1‑71 (MQ=255)
                            ctgcCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCgg                                        <  1:4568831/71‑1 (MQ=255)
                            ctgcCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCgg                                        >  1:3163480/1‑71 (MQ=255)
                            ctgcCAGTGTGGCTGATCCTCGCACCGCGCGACTATCTGGCAACCTTCCTGAAAATCGGCGTTAtcgtcg                                         <  1:5292467/70‑1 (MQ=255)
                            ctgcCAGTGTGGCTGATCCTCGCACCGCGCGACTATCTGGCAACCTTCCTGAAAATCGGCGTTAtcgtc                                          <  1:5149104/69‑1 (MQ=255)
                            ctgcCAGTGTGGCTGATCCTCGCACCGCGCGACTATCTGGCAACCTTCCTGAAAATCGGCGTTAtcgtc                                          <  1:1211863/69‑1 (MQ=255)
                               ccGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGa                                                           <  1:1612282/49‑1 (MQ=255)
                               ccGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCgg                                        <  1:1553514/68‑1 (MQ=255)
                               ccGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCgg                                        <  1:5232363/68‑1 (MQ=255)
                                 ggTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTg                                    <  1:1446727/70‑1 (MQ=255)
                                   tgtgGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGc                                  <  1:5483196/70‑1 (MQ=255)
                                   tgtgGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCg                                 <  1:457091/71‑1 (MQ=255)
                                    gtgGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCgg                                >  1:2327305/1‑71 (MQ=255)
                                     tgGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGaa                                                          <  1:2177273/44‑1 (MQ=255)
                                       gTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATGGTCGGt                                       <  1:5998/61‑1 (MQ=255)
                                       gTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCTGTCTGGCGGTAg                             <  1:1606371/71‑1 (MQ=255)
                                       gTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAg                             >  1:3797468/1‑71 (MQ=255)
                                        ttACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAgg                            >  1:2483854/1‑71 (MQ=255)
                                          aCTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATc                                                      >  1:5690135/1‑43 (MQ=255)
                                          aCTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGAc                                                 >  1:2137469/1‑48 (MQ=255)
                                          aCTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTg                                    <  1:5039293/61‑1 (MQ=255)
                                            tgctgGCCCCGCGTGACTACCTCTGTACCTTCCTGAAAATCGGGACTATCGTCGGt                                       <  1:3512216/56‑1 (MQ=255)
                                            tgctgGCCCCGCGTGACTACCTCTCTACCTTCCTGaaa                                                         <  1:4448642/38‑1 (MQ=255)
                                            tgctgGCCCCGCGTGACTACCTCTCTACCTTCCTGaaa                                                         <  1:1899124/38‑1 (MQ=255)
                                            tgctgGCCCCGCGTGACTACCTCTCTACCTTCCTGaaa                                                         <  1:1940238/38‑1 (MQ=255)
                                            tgctgGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGTGACTATCGTCGGt                                       <  1:1516846/56‑1 (MQ=255)
                                            tgctgGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGt                                       <  1:2109879/56‑1 (MQ=255)
                                              ctgGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCAtttt                      >  1:2013277/1‑71 (MQ=255)
                                              ctgGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCAtt                        <  1:4871700/69‑1 (MQ=255)
                                                 gCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGAt                   <  1:613552/71‑1 (MQ=255)
                                                 gCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGAt                   <  1:3925491/71‑1 (MQ=255)
                                                      gcgTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGAt                   <  1:1150380/66‑1 (MQ=255)
                                                      gcgTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGAt                   <  1:4726105/66‑1 (MQ=255)
                                                       cgTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCt                                     >  1:4729189/1‑47 (MQ=255)
                                                       cgTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGATTa                 >  1:3422983/1‑67 (MQ=255)
                                                        gTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGATTATgcgc            <  1:3111417/71‑1 (MQ=255)
                                                        gTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGATTATgcgc            >  1:2676299/1‑71 (MQ=255)
                                                              aCCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGATTATGCGCCCGACg      >  1:486545/1‑71 (MQ=255)
                                                               ccTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCt                                     >  1:2768379/1‑39 (MQ=255)
                                                                ctctctACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTg                     <  1:297900/54‑1 (MQ=255)
                                                                ctctctACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTg                     <  1:5159509/54‑1 (MQ=255)
                                                                ctctctACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGATTATGCGCCCGACGCt    <  1:3037071/71‑1 (MQ=255)
                                                                  ctctACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGATTa                 >  1:1308375/1‑56 (MQ=255)
                                                                  ctctACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGATTATGCGCCCGACGCTGa  >  1:98970/1‑71 (MQ=255)
                                                                  ctctACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGATTATGCGCCCGACGCTGa  >  1:4202142/1‑71 (MQ=255)
                                                                   |                                                                     
GGTGGGTTACGGTTTTGTGGCGGCGGTGCTGCCGGTGTGGTTACTGCTGGCCCCGCGTGACTACCTCTCTACCTTCCTGAAAATCGGGACTATCGTCGGTCTGGCGGTAGGCATTTTGATTATGCGCCCGACGCTGA  >  Exported/385570‑385706

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: