Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 385,780 T→C 26.2% F330F (TTT→TTC cstA → carbon starvation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported385,7800TC26.2% 156.7 / 61.4 99F330F (TTT→TTCcstAcarbon starvation protein
Reads supporting (aligned to +/- strand):  ref base T (34/39);  new base C (18/8);  total (52/47)
Fisher's exact test for biased strand distribution p-value = 6.69e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 103 total aligned reads displayed.

GCCTGCGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGA  >  Exported/385711‑385847
                                                                     |                                                                   
gCCTGCGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTa                                                                    >  1:3015739/1‑71 (MQ=255)
 ccTGCGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTAt                                                                   >  1:267907/1‑71 (MQ=255)
 ccTGCGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTAt                                                                   >  1:1176196/1‑71 (MQ=255)
   tGCGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTAt                                                                   <  1:5184631/69‑1 (MQ=255)
    gcgcTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCAc                                                                >  1:3218/1‑71 (MQ=255)
     cgcTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCAc                                                                >  1:5528319/1‑70 (MQ=255)
          aCCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGc                                                          >  1:153575/1‑71 (MQ=255)
           ccAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGcc                                                         <  1:412730/71‑1 (MQ=255)
            cAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTAt                                                                   >  1:1437279/1‑60 (MQ=255)
              aaTTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCtgt                                                      >  1:704952/1‑71 (MQ=255)
              aaTTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCtgt                                                      >  1:4366406/1‑71 (MQ=255)
               aTTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCtgtg                                                     <  1:534802/71‑1 (MQ=255)
                 ttGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCtga                                                      >  1:5220164/1‑67 (MQ=255)
                 ttGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGgc                                                   >  1:5664158/1‑71 (MQ=255)
                  tGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGgcg                                                  >  1:454788/1‑71 (MQ=255)
                      gATTGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGgt                                              <  1:5600569/71‑1 (MQ=255)
                      gATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGgt                                              <  1:2073417/71‑1 (MQ=255)
                        tGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGgtgt                                            <  1:381716/71‑1 (MQ=255)
                        tGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGgtg                                             >  1:757379/1‑70 (MQ=255)
                         ggCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTc                                           >  1:3297794/1‑71 (MQ=255)
                         ggCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTc                                           >  1:1851656/1‑71 (MQ=255)
                          gCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTc                                           <  1:3043332/70‑1 (MQ=255)
                          gCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCt                                          <  1:3771173/71‑1 (MQ=255)
                          gCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCt                                          <  1:208726/71‑1 (MQ=255)
                          gCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCt                                          >  1:1195516/1‑71 (MQ=255)
                          gCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCt                                          <  1:2961331/71‑1 (MQ=255)
                           cACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTg                                         >  1:1514140/1‑71 (MQ=255)
                            aCTGGCCCGGTGTGGTCGGGCAATGTGTTCCCCTTCCTGTTCATCACCATCGCCTGCGGCGCCgtc                                             <  1:5583916/66‑2 (MQ=255)
                            aCTGGCCCGGTGTGGTCGGGCAATGTGTTCCCCTTCCTGTTCATCACCATCGCCTGCGGCGCCgtc                                             <  1:3076186/66‑2 (MQ=255)
                             cTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCtg                                                       <  1:3663082/55‑1 (MQ=255)
                             cTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCtg                                                       <  1:4638015/55‑1 (MQ=255)
                             cTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGc                                       >  1:1656502/1‑71 (MQ=255)
                             cTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGc                                       >  1:3721522/1‑71 (MQ=255)
                              tGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCtg                                                       <  1:5119357/54‑1 (MQ=255)
                               ggCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATc                                                                  >  1:5134494/1‑42 (MQ=255)
                                gCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCC‑‑TGGCGCGGTGTCTGGCTTc                                    <  1:2628831/69‑1 (MQ=255)
                                    ggTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCt                                                        >  1:4413748/1‑47 (MQ=255)
                                      tATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCTtgtg                                                     <  1:175695/48‑1 (MQ=255)
                                      tATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTc                                    <  1:4039023/65‑1 (MQ=255)
                                        tGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGgt                                              <  1:5547667/53‑1 (MQ=255)
                                        tGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGc                                       <  1:5280871/60‑1 (MQ=255)
                                          gACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCtg                                                       >  1:1997239/1‑42 (MQ=255)
                                          gACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTg                           <  1:1045122/70‑1 (MQ=255)
                                          gACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTg                           <  1:4515735/70‑1 (MQ=255)
                                           aCCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCAGTGGCGCGGTGTCTgg                                        <  1:3608653/56‑1 (MQ=255)
                                             cGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTc                                           <  1:4457964/51‑1 (MQ=255)
                                              ggTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGAtctc                      <  1:5356668/71‑1 (MQ=255)
                                               gTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATctct                     <  1:3691129/71‑1 (MQ=255)
                                                tAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCtctt                    <  1:4003777/71‑1 (MQ=255)
                                                tAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCtctt                    <  1:3370116/71‑1 (MQ=255)
                                                 aaCCTGTTCGCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCtcttc                   >  1:3309006/1‑71 (MQ=255)
                                                 aaCCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTg                           >  1:1059454/1‑63 (MQ=255)
                                                 aaCCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCtcttc                   >  1:5359783/1‑71 (MQ=255)
                                                  aCCTGTTCCCGTTCCTGTTTATCACCATCGCCtgt                                                      <  1:3098531/35‑1 (MQ=255)
                                                  aCCTGTTCCCGTTCCTGTTTATCACCATCGCCtgt                                                      <  1:3837791/35‑1 (MQ=255)
                                                   tcTGTTCCCGTTCCTGTTCATCACCATCGCCTGTGGTGCGgt                                              <  1:652291/41‑1 (MQ=38)
                                                    cTGTTCCCGTTCCTGTTTTTCACCATCGCCTGTGGCGCGGTGTCTGGCTTc                                    <  1:754599/51‑1 (MQ=255)
                                                    cTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTc                                    <  1:705027/51‑1 (MQ=255)
                                                    cTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTc                                    <  1:423090/51‑1 (MQ=255)
                                                    cTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTc                                    <  1:1546709/51‑1 (MQ=255)
                                                    cTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTgg                <  1:3770457/71‑1 (MQ=255)
                                                    cTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTgg                <  1:4010256/71‑1 (MQ=255)
                                                    cTGTTCCCGTTCCTGTTCATCACCATCGCCTGTGGTGCGgt                                              <  1:1652347/41‑1 (MQ=38)
                                                    cTGTTCCCGTTCCTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGAt                         >  1:3604457/1‑62 (MQ=255)
                                                    cTGTTCCCGTTCCTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCgg                >  1:3630361/1‑71 (MQ=255)
                                                    cTGTTCCCGTTCCTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCgg                >  1:344535/1‑71 (MQ=255)
                                                     tGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTgg                >  1:316871/1‑70 (MQ=255)
                                                     tGTTCCCGTTCCTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCtcttccg                 <  1:379824/69‑3 (MQ=255)
                                                     tGTTCCCGTTCCTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCgg                >  1:3299243/1‑70 (MQ=255)
                                                     tGTTCCCCTTCCTGTTCATCACCATCGCCTGCGGCGCCGTCTCGGGCTTcc                                   >  1:3721054/1‑51 (MQ=19)
                                                     tGTTCCCCTTCCTGTTCATCACCATCGCCTGCGGCGCCGTCTCGGGCTTCCa                                  >  1:204728/1‑52 (MQ=25)
                                                     tGTTCCCCTTCCTGTTCATCACCATCGCCTGCGGCGCCGTCTCGGGCTTCCa                                  >  1:4937681/1‑52 (MQ=25)
                                                     tGTTCCCCTTCCTGTTCATCACCATCGCCTGCGGCGCCGTCTCGGGCTTCCa                                  >  1:179118/1‑52 (MQ=25)
                                                      gTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCa                                  >  1:3396405/1‑51 (MQ=255)
                                                       ttCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCa                                  >  1:1626343/1‑50 (MQ=255)
                                                       ttCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCa                                  >  1:2207877/1‑50 (MQ=255)
                                                       ttCCCGTTCCTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTAc             >  1:2880985/1‑71 (MQ=255)
                                                        tcgcGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGAc             >  1:2441857/4‑70 (MQ=255)
                                                        tCCCGTTCCTGTTCATCACCATCGCCTGTGGtgcg                                                >  1:1075373/1‑35 (MQ=37)
                                                         cccGTTCCTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTc                                    >  1:1554944/1‑46 (MQ=255)
                                                         cccGTTCATGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTc                                    >  1:1115606/1‑46 (MQ=255)
                                                          ccGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCAc          <  1:5355415/71‑1 (MQ=255)
                                                            gTTCCTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGc                                       >  1:2734362/1‑40 (MQ=37)
                                                            gTTCCTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGc        >  1:201029/1‑71 (MQ=255)
                                                            gTTCCTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGc        >  1:4703036/1‑71 (MQ=255)
                                                            gTTCCTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGc        >  1:5588902/1‑71 (MQ=255)
                                                             ttCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGcc       <  1:168692/71‑1 (MQ=255)
                                                             ttCCTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGcc       <  1:3677951/71‑1 (MQ=255)
                                                              tCCTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCtcttc                   >  1:2926318/1‑58 (MQ=255)
                                                               ccTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGa                          >  1:3575214/1‑50 (MQ=255)
                                                                cTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGAt                         >  1:1272292/1‑50 (MQ=255)
                                                                cTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGAt                         >  1:1213713/1‑50 (MQ=255)
                                                                cTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGAt                         >  1:577523/1‑50 (MQ=255)
                                                                cTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGAt                         >  1:4105061/1‑50 (MQ=255)
                                                                cTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGccaa     <  1:345194/70‑3 (MQ=255)
                                                                 tGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCtcttct                  <  1:2502230/56‑1 (MQ=255)
                                                                   tttATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATctct                     <  1:1289056/51‑1 (MQ=255)
                                                                   tttATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGa  <  1:1576820/70‑1 (MQ=255)
                                                                   tttATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGa  <  1:1661703/70‑1 (MQ=255)
                                                                   tttATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGa  <  1:5116961/70‑1 (MQ=255)
                                                                     |                                                                   
GCCTGCGCTGACCAAATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGA  >  Exported/385711‑385847

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: