Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 385,795 T→C 5.9% C335C (TGT→TGC cstA → carbon starvation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported385,7950TC 5.9% 290.9 / 6.1 101C335C (TGT→TGCcstAcarbon starvation protein
Reads supporting (aligned to +/- strand):  ref base T (47/48);  new base C (4/2);  total (51/50)
Fisher's exact test for biased strand distribution p-value = 6.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.83e-01

Only 100 of 117 total aligned reads displayed.

AATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGG  >  Exported/385725‑385864
                                                                      |                                                                     
aaTTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCtgt                                                                       >  1:4366406/1‑71 (MQ=255)
 aTTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCtgtg                                                                      <  1:534802/71‑1 (MQ=255)
   ttGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGgc                                                                    >  1:5664158/1‑71 (MQ=255)
    tGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGgcg                                                                   >  1:454788/1‑71 (MQ=255)
        gATTGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGgt                                                               <  1:5600569/71‑1 (MQ=255)
        gATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGgt                                                               <  1:2073417/71‑1 (MQ=255)
          tGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGgtgt                                                             <  1:381716/71‑1 (MQ=255)
          tGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGgtg                                                              >  1:757379/1‑70 (MQ=255)
           ggCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTc                                                            >  1:3297794/1‑71 (MQ=255)
           ggCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTc                                                            >  1:1851656/1‑71 (MQ=255)
            gCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTc                                                            <  1:3043332/70‑1 (MQ=255)
            gCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCt                                                           <  1:3771173/71‑1 (MQ=255)
            gCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCt                                                           <  1:2961331/71‑1 (MQ=255)
            gCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCt                                                           >  1:1195516/1‑71 (MQ=255)
            gCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCt                                                           <  1:208726/71‑1 (MQ=255)
             cACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTg                                                          >  1:1514140/1‑71 (MQ=255)
              aCTGGCCCGGTGTGGTCGGGCAATGTGTTCCCCTTCCTGTTCATCACCATCGCCTGCGGCGCCgtc                                                              <  1:3076186/66‑2 (MQ=255)
               cTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGc                                                        >  1:1656502/1‑71 (MQ=255)
                  gCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCC‑‑TGGCGCGGTGTCTGGCTTc                                                     <  1:2628831/69‑1 (MQ=255)
                        tATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCTtgtg                                                                      <  1:175695/48‑1 (MQ=255)
                        tATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTc                                                     <  1:4039023/65‑1 (MQ=255)
                          tGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGgt                                                               <  1:5547667/53‑1 (MQ=255)
                          tGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGc                                                        <  1:5280871/60‑1 (MQ=255)
                            gACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTg                                            <  1:1045122/70‑1 (MQ=255)
                            gACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTg                                            <  1:4515735/70‑1 (MQ=255)
                             aCCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCAGTGGCGCGGTGTCTgg                                                         <  1:3608653/56‑1 (MQ=255)
                               cGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTc                                                            <  1:4457964/51‑1 (MQ=255)
                                ggTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGAtctc                                       <  1:5356668/71‑1 (MQ=255)
                                 gTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATctct                                      <  1:3691129/71‑1 (MQ=255)
                                  tAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCtctt                                     <  1:3370116/71‑1 (MQ=255)
                                   aaCCTGTTCGCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCtcttc                                    >  1:3309006/1‑71 (MQ=255)
                                   aaCCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTg                                            >  1:1059454/1‑63 (MQ=255)
                                   aaCCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCtcttc                                    >  1:5359783/1‑71 (MQ=255)
                                    aCCTGTTCCCGTTCCTGTTTATCACCATCGCCtgt                                                                       <  1:3837791/35‑1 (MQ=255)
                                    aCCTGTTCCCGTTCCTGTTTATCACCATCGCCtgt                                                                       <  1:889498/35‑1 (MQ=255)
                                    aCCTGTTCCCGTTCCTGTTTATCACCATCGCCtgt                                                                       <  1:4042647/35‑1 (MQ=255)
                                     tcTGTTCCCGTTCCTGTTCATCACCATCGCCTGTGGTGCGgt                                                               <  1:652291/41‑1 (MQ=38)
                                      cTGTTCCCGTTCCTGTTTTTCACCATCGCCTGTGGCGCGGTGTCTGGCTTc                                                     <  1:754599/51‑1 (MQ=255)
                                      cTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTc                                                     <  1:1546709/51‑1 (MQ=255)
                                      cTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTc                                                     <  1:705027/51‑1 (MQ=255)
                                      cTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTgg                                 <  1:3770457/71‑1 (MQ=255)
                                      cTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTgg                                 <  1:4010256/71‑1 (MQ=255)
                                      cTGTTCCCGTTCCTGTTCATCACCATCGCCTGTGGTGCGgt                                                               <  1:1652347/41‑1 (MQ=38)
                                      cTGTTCCCGTTCCTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCgg                                 >  1:3630361/1‑71 (MQ=255)
                                      cTGTTCCCGTTCCTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCgg                                 >  1:344535/1‑71 (MQ=255)
                                       tGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTgg                                 >  1:316871/1‑70 (MQ=255)
                                       tGTTCCCGTTCCTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCtcttccg                                  <  1:379824/69‑3 (MQ=255)
                                       tGTTCCCGTTCCTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCgg                                 >  1:3299243/1‑70 (MQ=255)
                                       tGTTCCCCTTCCTGTTCATCACCATCGCCTGCGGCGCCGTCTCGGGCTTcc                                                    >  1:3721054/1‑51 (MQ=19)
                                       tGTTCCCCTTCCTGTTCATCACCATCGCCTGCGGCGCCGTCTCGGGCTTCCa                                                   >  1:179118/1‑52 (MQ=25)
                                       tGTTCCCCTTCCTGTTCATCACCATCGCCTGCGGCGCCGTCTCGGGCTTCCa                                                   >  1:4937681/1‑52 (MQ=25)
                                        gTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCa                                                   >  1:3396405/1‑51 (MQ=255)
                                         ttCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCa                                                   >  1:1626343/1‑50 (MQ=255)
                                         ttCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCa                                                   >  1:2207877/1‑50 (MQ=255)
                                         ttCCCGTTCCTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTAc                              >  1:2880985/1‑71 (MQ=255)
                                          tcgcGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGAc                              >  1:2441857/4‑70 (MQ=255)
                                          tCCCGTTCCTGTTCATCACCATCGCCTGTGGtgcg                                                                 >  1:1075373/1‑35 (MQ=37)
                                           cccGTTCATGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTc                                                     >  1:1115606/1‑46 (MQ=255)
                                            ccGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCAc                           <  1:5355415/71‑1 (MQ=255)
                                              gTTCCTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGc                         >  1:5588902/1‑71 (MQ=255)
                                              gTTCCTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGc                         >  1:4703036/1‑71 (MQ=255)
                                              gTTCCTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGc                         >  1:201029/1‑71 (MQ=255)
                                               ttCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGcc                        <  1:168692/71‑1 (MQ=255)
                                               ttCCTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGcc                        <  1:3677951/71‑1 (MQ=255)
                                                tCCTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCtcttc                                    >  1:2926318/1‑58 (MQ=255)
                                                 ccTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGa                                           >  1:3575214/1‑50 (MQ=255)
                                                  cTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGAt                                          >  1:1272292/1‑50 (MQ=255)
                                                  cTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGAt                                          >  1:4105061/1‑50 (MQ=255)
                                                  cTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGAt                                          >  1:577523/1‑50 (MQ=255)
                                                  cTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGccaa                      <  1:2874781/70‑3 (MQ=255)
                                                  cTGTTCATCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGccaa                      <  1:345194/70‑3 (MQ=255)
                                                   tGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCtcttct                                   <  1:2502230/56‑1 (MQ=255)
                                                     tttATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATctct                                      <  1:1289056/51‑1 (MQ=255)
                                                     tttATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGa                   <  1:1576820/70‑1 (MQ=255)
                                                     tttATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGa                   <  1:1661703/70‑1 (MQ=255)
                                                     tttATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGa                   <  1:5116961/70‑1 (MQ=255)
                                                         tCACCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTAcga                            <  1:2420229/57‑3 (MQ=255)
                                                           aCCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTgg             >  1:3700686/1‑70 (MQ=255)
                                                           aCCATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGc            >  1:4549970/1‑71 (MQ=255)
                                                           aCCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGc            >  1:2658140/1‑71 (MQ=255)
                                                           aCCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGc            >  1:5469831/1‑71 (MQ=255)
                                                            ccATCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTAcga                            <  1:1188041/54‑3 (MQ=255)
                                                               tCGCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTgg             <  1:4625930/66‑1 (MQ=255)
                                                               tCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaac        >  1:1952477/1‑71 (MQ=255)
                                                                 gCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGctaatg       <  1:5170441/70‑3 (MQ=255)
                                                                 gCCTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGctaatg       >  1:5027682/1‑68 (MQ=255)
                                                                 gCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacg       <  1:2474919/70‑1 (MQ=255)
                                                                 gCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacg       <  1:4227084/70‑1 (MQ=255)
                                                                 gCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacg       <  1:133727/70‑1 (MQ=255)
                                                                 gCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacg       <  1:2238044/70‑1 (MQ=255)
                                                                 gCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcgaacg       <  1:3113432/70‑1 (MQ=255)
                                                                 gCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGcg           >  1:3418768/1‑66 (MQ=255)
                                                                  ccTGTGGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGctaatga      >  1:1528704/1‑70 (MQ=255)
                                                                   cTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAg    >  1:2125439/1‑71 (MQ=255)
                                                                      tgGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGctaatgaaacc  <  1:89464/70‑4 (MQ=255)
                                                                      tgGTGCGGTATCTGGCTTCCACGCGCTGATCTCTTCCGGTACGACGCCAAAACTGCTGGct           <  1:1943278/61‑2 (MQ=255)
                                                                      tgGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGa                   >  1:5612503/1‑53 (MQ=255)
                                                                      tgGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGa                   >  1:4199975/1‑53 (MQ=255)
                                                                      tgGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGa                   >  1:2217885/1‑53 (MQ=255)
                                                                      tgGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGa                   >  1:3948685/1‑53 (MQ=255)
                                                                      |                                                                     
AATTTGTCGATGGCACTGGCCCGGTATGGACCGGTAACCTGTTCCCGTTCCTGTTTATCACCATCGCCTGTGGCGCGGTGTCTGGCTTCCATGCGCTGATCTCTTCTGGGACCACGCCGAAGATGCTGGCGAACGAAGGG  >  Exported/385725‑385864

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: