Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 132,241 G→T 23.0% L178L (CTG→CTT) ‡ yadD → predicted transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported132,2410GT23.0% 211.3 / 40.6 95L178L (CTG→CTT) ‡yadDpredicted transposase
Reads supporting (aligned to +/- strand):  ref base G (32/41);  new base T (14/8);  total (46/49)
Fisher's exact test for biased strand distribution p-value = 1.44e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.33e-01

GTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAGG  >  Exported/132173‑132309
                                                                    |                                                                    
gtggATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCa                                                                    <  1:5028866/71‑1 (MQ=255)
  ggATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaa                                                                  <  1:879856/71‑1 (MQ=255)
   gATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaaa                                                                 <  1:3186170/71‑1 (MQ=255)
   gATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaaa                                                                 <  1:2104096/71‑1 (MQ=255)
   gATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaa                                                                  <  1:5527922/70‑1 (MQ=255)
    atatCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaaa                                                                 <  1:2659004/70‑1 (MQ=255)
       tCACCATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCa                                                              >  1:4331714/1‑70 (MQ=255)
        cACCATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCata                                                            >  1:4456591/1‑71 (MQ=255)
        cACCATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCat                                                             >  1:3151006/1‑70 (MQ=255)
        cACCATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCat                                                             >  1:5139991/1‑70 (MQ=255)
        cACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACata                                                            >  1:137935/1‑71 (MQ=255)
         aCCATCGTACCTGACGATGAGATCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCata                                                            <  1:1682564/70‑1 (MQ=255)
         aCCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACatat                                                           <  1:5411175/71‑1 (MQ=255)
            aTCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCg                                                        <  1:4109234/71‑1 (MQ=255)
             tCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGc                                                       >  1:1724271/1‑71 (MQ=255)
             tCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGc                                                       <  1:2811198/71‑1 (MQ=255)
             tCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGc                                                       <  1:3733406/71‑1 (MQ=255)
             tCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGc                                                       <  1:1990572/71‑1 (MQ=255)
               acacCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGcc                                                      <  1:5225852/70‑1 (MQ=255)
               acacCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGcc                                                      <  1:1456262/70‑1 (MQ=255)
                 acCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaaa                                                                 <  1:4922131/57‑1 (MQ=255)
                 acCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaaa                                                                 <  1:1591358/57‑1 (MQ=255)
                   cGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAgcgc                                                 <  1:1982600/71‑1 (MQ=255)
                   cGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAgcgc                                                 >  1:5603944/1‑71 (MQ=255)
                   cGGATGACGAAATCATGCAACATAGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAgcg                                                  >  1:4314735/1‑70 (MQ=255)
                    ggATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACa                                                              <  1:4411448/57‑1 (MQ=255)
                    ggATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACa                                                              <  1:5499548/57‑1 (MQ=255)
                      aTGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaat                                                                 <  1:1765041/52‑2 (MQ=255)
                       tGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGc                                                       <  1:5480886/61‑1 (MQ=255)
                       tGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACt                                             <  1:5105414/71‑1 (MQ=255)
                         aCGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACtt                                            >  1:4311932/1‑70 (MQ=255)
                            agaTCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGCGACCGCGATTTAAtc                                        >  1:5264174/3‑70 (MQ=255)
                             aaTCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGt                                       <  1:4346016/71‑1 (MQ=255)
                              aTCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGCGACcgcg                                                <  1:1981471/61‑1 (MQ=255)
                              aTCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGCGACCGCGATTTAAtcgg                                      <  1:3144933/71‑4 (MQ=255)
                               tCATGCAACATCGGCGTATAGCTCTGCTTGAACTGATTCAAAAGCATATTCGCGACCGCGat                                              <  1:437409/62‑2 (MQ=255)
                               tCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGCGACCGCGat                                              <  1:3389478/62‑2 (MQ=255)
                               tCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGCGACCGCGat                                              <  1:5381492/62‑2 (MQ=255)
                               tCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGCGACCGCGAttt                                            <  1:4614380/64‑1 (MQ=255)
                               tCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGCGACCGCGATTTAAtcgg                                      <  1:4086207/70‑4 (MQ=255)
                               tCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaaaa                                                                <  1:3964826/44‑1 (MQ=255)
                               tCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaaaa                                                                <  1:5304759/44‑1 (MQ=255)
                               tCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaaaa                                                                <  1:2386059/44‑1 (MQ=255)
                                cATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCata                                                            >  1:1096838/1‑47 (MQ=255)
                                cATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCat                                                             >  1:3949494/1‑46 (MQ=255)
                                cATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCat                                                             >  1:3643736/1‑46 (MQ=255)
                                cATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCat                                                             >  1:3007904/1‑46 (MQ=255)
                                cATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGCGACCGCGat                                              >  1:2371657/1‑60 (MQ=255)
                                cATGCAACATAGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGCGACCGCGat                                              >  1:3248330/1‑60 (MQ=255)
                                 aTGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACa                                                              >  1:176267/1‑44 (MQ=255)
                                  tGCAACATCGGCGGATTGCGATTCTCGAACTACTGCaaa                                                                  <  1:202621/39‑1 (MQ=255)
                                   gCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGcga                                                     >  1:2947347/1‑49 (MQ=255)
                                    cAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAgcg                                                  >  1:1678405/1‑53 (MQ=255)
                                    cAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCt                                >  1:121453/1‑71 (MQ=255)
                                    cAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCt                                <  1:4014833/71‑1 (MQ=255)
                                    cAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCt                                >  1:1755295/1‑71 (MQ=255)
                                     aaCATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGCGACCGCGATTTa                                           >  1:2550432/1‑59 (MQ=255)
                                     aaCATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGCGACCGCGATTTa                                           >  1:2398642/1‑59 (MQ=255)
                                      aCATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAgcgc                                                 <  1:825848/52‑1 (MQ=255)
                                        aTCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTaa                                          >  1:4756399/1‑57 (MQ=255)
                                        aTCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTaa                                          >  1:5389881/1‑57 (MQ=255)
                                        aTCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAg                            >  1:3152257/1‑71 (MQ=255)
                                          cggcggATTGCGATTCTCGAACTACTGCAAAAACatat                                                           >  1:2487730/1‑38 (MQ=255)
                                          cggcggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAAt                                         >  1:5388259/1‑56 (MQ=255)
                                          cggcggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAAt                                         >  1:4727016/1‑56 (MQ=255)
                                          cggcggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAAt                                         >  1:4733468/1‑56 (MQ=255)
                                           ggcggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCaa                         <  1:1726524/71‑1 (MQ=255)
                                           ggcggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCaa                         >  1:549698/1‑71 (MQ=255)
                                           ggcggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCaa                         <  1:1932043/71‑1 (MQ=255)
                                           ggcggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCaa                         >  1:5025523/1‑71 (MQ=255)
                                              ggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACt                       <  1:1854585/70‑1 (MQ=255)
                                                     gATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCtt                               <  1:1875035/55‑1 (MQ=255)
                                                     gATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCtt                               <  1:1957148/55‑1 (MQ=255)
                                                       ttCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCt              >  1:5450095/1‑70 (MQ=255)
                                                       ttCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTg             <  1:2192961/71‑1 (MQ=255)
                                                        tctcGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGa            >  1:1205416/1‑71 (MQ=255)
                                                        tctcGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGa            >  1:3072992/1‑71 (MQ=255)
                                                         ctcGAACTACTGCAAAAACATATTCGCCAGCGCGACTTaa                                          >  1:1582883/1‑40 (MQ=255)
                                                         ctcGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCaa                         >  1:3476834/1‑57 (MQ=255)
                                                         ctcGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCaa                         >  1:4439828/1‑57 (MQ=255)
                                                            gAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTAt                                    >  1:1217928/1‑43 (MQ=255)
                                                            gAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGAt           <  1:498423/68‑1 (MQ=255)
                                                            gAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATc          <  1:2636217/69‑1 (MQ=255)
                                                              actactGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATcgcc       >  1:4945734/1‑68 (MQ=255)
                                                              actactGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATcgac       >  1:4301254/1‑70 (MQ=255)
                                                              actactGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATcgac       <  1:27477/70‑1 (MQ=255)
                                                                 actGCAAAAACATATTCGCCAGCGCGACTTTATGt                                       <  1:2813211/35‑1 (MQ=255)
                                                                 actGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAg   >  1:4110214/1‑71 (MQ=255)
                                                                  ctGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAAc                        >  1:2780334/1‑49 (MQ=255)
                                                                  ctGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAAc                        >  1:820289/1‑49 (MQ=255)
                                                                  ctGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATc          <  1:1448313/63‑1 (MQ=255)
                                                                  ctGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGaa    >  1:723550/1‑69 (MQ=255)
                                                                  ctGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAgg  >  1:3767974/1‑71 (MQ=255)
                                                                   tGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGc                           <  1:5630840/45‑1 (MQ=255)
                                                                   tGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGt                    <  1:2358735/52‑1 (MQ=255)
                                                                    gCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATcgacga     >  1:5393177/1‑66 (MQ=255)
                                                                    gCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATcgacga     >  1:4396399/1‑66 (MQ=255)
                                                                    |                                                                    
GTGGATATCACCATCACACCGGATGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAGG  >  Exported/132173‑132309

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: