Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 386,230 A→C 8.4% A480A (GCA→GCC cstA → carbon starvation protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported386,2300AC 8.4% 207.4 / 11.1 83A480A (GCA→GCCcstAcarbon starvation protein
Reads supporting (aligned to +/- strand):  ref base A (35/41);  new base C (3/4);  total (38/45)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

TTCTGCACGGCGCGCTGGGCGGCATGATGGATGTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGTGCTGCGCGCTTTATGTTGCAGGATCTGCTG  >  Exported/386160‑386296
                                                                      |                                                                  
ttCTGCACGGCGCGCTGGGCGGCATGATGGATGTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCa                                                                    >  1:5432269/1‑71 (MQ=255)
ttCTGCACGGCGCGCTGGGCGGCATGATGGATGTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCa                                                                    <  1:2568366/71‑1 (MQ=255)
  cTGCACGGCGCGCTGGGCGGCATGATGGATGTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACt                                                                  >  1:4523335/1‑71 (MQ=255)
     cACGGCGCGCTGGGCGGCATGATGGATGTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACt                                                                  <  1:463774/68‑1 (MQ=255)
     cACGGCGCGCTGGGCGGCATGATGGATGTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGtt                                                               >  1:3220209/1‑71 (MQ=255)
      aCGGCGCGCTGGGCGGCATGATGGATGTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGttt                                                              <  1:1198125/71‑1 (MQ=255)
       cGGCGCGCTGGGCGGCATGATGGATGTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTa                                                             >  1:1146638/1‑71 (MQ=255)
       cGGCGCGCTGGGCGGCATGATGGATGTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTa                                                             <  1:950548/71‑1 (MQ=255)
         gcgcgcTGGGCGGCATGATGGATGTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTa                                                             >  1:2366779/1‑69 (MQ=255)
              cTGGGCGGCATGATGGATGTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGAc                                                      >  1:4307277/1‑71 (MQ=255)
               tGGGCGGCATGATGGATGTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGAc                                                      >  1:5597332/1‑70 (MQ=255)
                gggCGGCATGATGGATGTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGAcgg                                                    >  1:3454238/1‑71 (MQ=255)
                      cATGATGGATGTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCa                                                                    >  1:1252992/1‑49 (MQ=255)
                      cATGATGGATGTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCa                                                                    >  1:5668714/1‑49 (MQ=255)
                      cATGATGGATGTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCa                                                                    >  1:1570879/1‑49 (MQ=255)
                       atgatgGATGTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGa                                             >  1:1404732/1‑71 (MQ=255)
                       atgatgGATGTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGa                                             >  1:3274472/1‑71 (MQ=255)
                          atggatgTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCAc                                                                   >  1:3907023/1‑46 (MQ=255)
                          atggatgTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGAcggcgg                                                 >  1:1608238/1‑64 (MQ=255)
                          atggatgTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATg                                           >  1:5324654/1‑70 (MQ=255)
                          atggatgTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATg                                           >  1:5325828/1‑70 (MQ=255)
                          atggatgTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATg                                           <  1:1738957/70‑1 (MQ=255)
                          atggatgTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGc                                          <  1:4526423/71‑1 (MQ=255)
                          atggatgTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGc                                          <  1:3084162/71‑1 (MQ=255)
                          atggatgTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGc                                          <  1:4406319/71‑1 (MQ=255)
                             gatgTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGa                                             >  1:4132793/1‑65 (MQ=255)
                             gatgTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAgg                                       <  1:1297316/71‑1 (MQ=255)
                              atgTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGTTGCAGGt                                      <  1:33828/71‑1 (MQ=255)
                              atgTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAgg                                       >  1:5211871/1‑70 (MQ=255)
                                   gCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTAcgcg                                 <  1:3166406/71‑1 (MQ=255)
                                   gCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTAcgcg                                 <  1:293709/71‑1 (MQ=255)
                                   gCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTAcgcg                                 <  1:5476324/71‑1 (MQ=255)
                                   gCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTAcgcg                                 <  1:2668341/71‑1 (MQ=255)
                                   gCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTAcgcg                                 <  1:806370/71‑1 (MQ=255)
                                   gCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTAcgc                                  <  1:4521844/70‑1 (MQ=255)
                                    gcttCTGGTATCACTTCGGTATTCTGTTCGAAGCCCTGTTCATCCTGACCGCGCTGGATGCGGGTAcccg                                 >  1:3733837/3‑70 (MQ=255)
                                    gcttCTGGTATCACTTCGGTATTCTGTTCGAAGCCCTGTTCATCCTGACCGCGCTGGATGCGGGTACCCGt                                <  1:305927/69‑1 (MQ=255)
                                    gcttCTGGTATCACTTCGGTATTCTGTTCGAAGCCCTGTTCATCCTGACCGCGCTGGATGCGGGTACCCGt                                <  1:3008848/69‑1 (MQ=255)
                                    gcgtCTGGTATCACTTCGGTATTCTGTTCGAAGCCCTGTTCATCCTGACCGCGCTGGATGCGGGt                                      <  1:5138441/62‑1 (MQ=255)
                                    cGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTAcgcg                                 <  1:3595086/70‑1 (MQ=255)
                                    cGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGt                                <  1:5282299/71‑1 (MQ=255)
                                    cGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGt                                <  1:3870259/71‑1 (MQ=255)
                                    cGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGt                                <  1:4382262/71‑1 (MQ=255)
                                     gTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGtg                               <  1:731110/71‑1 (MQ=255)
                                     gTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGtg                               <  1:3025731/71‑1 (MQ=255)
                                      ttCTGGTATCACTTCGCCATTTTGTTTGAAGCACt                                                                  >  1:182072/1‑35 (MQ=255)
                                      ttCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGttt                                                              <  1:4219324/39‑1 (MQ=255)
                                      ttCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTAt                                                            <  1:3386721/41‑1 (MQ=255)
                                      ttCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGc                                          <  1:3724063/59‑1 (MQ=255)
                                      ttCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGtgc                              <  1:472205/71‑1 (MQ=255)
                                        cTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTAt                                                            >  1:656532/1‑39 (MQ=255)
                                        cTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGAcgg                                                    >  1:558162/1‑47 (MQ=255)
                                        cTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCa                                         >  1:5628888/1‑58 (MQ=255)
                                        cTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGtgct                             <  1:330016/70‑1 (MQ=255)
                                         tGGTATCACTTCGGTATTCTGTTCGAAGCCCTGTTCATCCTGACCGCGCTGGATGc                                          <  1:3908714/56‑1 (MQ=255)
                                         tGGTATCACTTCGGTATTCTGTTCGAAGCCCTGTTCATCCTGACCGCGCTGGATGCGGGTACCCGttctgg                           >  1:4394724/1‑70 (MQ=255)
                                         tGGTATCACTTCGGTATTCTGTTCGAAGCCCTGTTCATCCTGACCGCGCTGGATGCGGGTACCCGttctgg                           >  1:3426748/1‑70 (MQ=255)
                                             aTCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGTGCTgcgcgc                       >  1:2687181/1‑71 (MQ=255)
                                             aTCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGTGCTgcg                          >  1:1395470/1‑68 (MQ=255)
                                             aTCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGTGCTgcg                          >  1:5136372/1‑68 (MQ=255)
                                               cACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAg                                        >  1:3783402/1‑52 (MQ=255)
                                               cACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAg                                        >  1:5262176/1‑52 (MQ=255)
                                                aCTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGTGCTGCGCGCtt                     >  1:627540/1‑70 (MQ=255)
                                                  ttCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTAcg                                   <  1:714393/54‑1 (MQ=255)
                                                  ttCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTAcg                                   <  1:4238556/54‑1 (MQ=255)
                                                   tCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGTGCTGCGCGCTTTATg                 >  1:1132488/1‑71 (MQ=255)
                                                    cGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGTGCTGCGCGCTTTAt                  <  1:5523042/69‑1 (MQ=255)
                                                     gCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGtgct                             >  1:3073553/1‑57 (MQ=255)
                                                     gCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGtgct                             >  1:1514813/1‑57 (MQ=255)
                                                     gCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGtgct                             >  1:2355952/1‑57 (MQ=255)
                                                      ccATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGTGCTGCGCGCTTTATGTTg              <  1:5128654/71‑1 (MQ=255)
                                                       cATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGTGCTGCGCGCTTTATGTTGc             <  1:444402/71‑1 (MQ=255)
                                                       cATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGTGCTGCGCGCTTTATGTTGc             <  1:1934363/71‑1 (MQ=255)
                                                         ttttGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGTGCTGCGCGCTTTATGTTGCAg           <  1:3063722/71‑1 (MQ=255)
                                                          tttgtttgAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGTGCTGCGCGCTTTATGTTGCAg           >  1:1372550/1‑70 (MQ=255)
                                                           ttgtttgAAGCACTGTTTATTCTGACGGCGGTGGa                                             >  1:1346262/1‑35 (MQ=255)
                                                           ttgtttgAAGCACTGTTTATTCTGACGGCGGTGGa                                             >  1:5313221/1‑35 (MQ=255)
                                                           ttgtttgAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTa                                     >  1:4652966/1‑43 (MQ=255)
                                                           ttgtttgAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGtgctgc                           >  1:5369582/1‑53 (MQ=255)
                                                           ttgtttgAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGTGCTGCGCGCTTTAt                  >  1:671389/1‑62 (MQ=255)
                                                                 gAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGTGCTgcgc                         <  1:4928386/49‑1 (MQ=255)
                                                                 gAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGTGCTgcgc                         <  1:4128685/49‑1 (MQ=255)
                                                                 gAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGTGCTGCGCGCTTTa                   <  1:181016/55‑1 (MQ=255)
                                                                 gAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGTGCTGCGCGCTTTATGTTGCAGGATctgc    <  1:2314553/70‑1 (MQ=255)
                                                                 gAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGTGATGCGCGCTTTa                   <  1:927550/55‑1 (MQ=255)
                                                                 gAAGCACTGTTTATTTGACGGCGGTGGATGCAGGTACGCGTGCTGCGCGCTTTa                   <  1:1710832/54‑1 (MQ=255)
                                                                  aaGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGTGCTGCGCGCTTTATGTTGCAGGATctgctg  <  1:4754531/71‑1 (MQ=255)
                                                                  aaGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGTGCTGCGCGCTTTATGTTGCAGGATctgct   <  1:1764216/70‑1 (MQ=255)
                                                                      |                                                                  
TTCTGCACGGCGCGCTGGGCGGCATGATGGATGTGGCGTTCTGGTATCACTTCGCCATTTTGTTTGAAGCACTGTTTATTCTGACGGCGGTGGATGCAGGTACGCGTGCTGCGCGCTTTATGTTGCAGGATCTGCTG  >  Exported/386160‑386296

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: