Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	132,265	C→T	5.2%	D186D (GAC→GAT)	yadD →	predicted transposase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	132,265	0	C	T	5.2%	292.0 / 3.4	95	D186D (GAC→GAT)	yadD	predicted transposase
Reads supporting (aligned to +/- strand):  ref base C (45/45);  new base T (3/2);  total (48/47)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.90e-01

TGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAGGGTACACTAGCGGAAGTCAGTTAGTTG  >  Exported/132196‑132335                                                                      |                                                                       tGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACt                                                                       <  1:5105414/71‑1 (MQ=255)   aCGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACtt                                                                      >  1:4311932/1‑70 (MQ=255)      agaTCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGCGACCGCGATTTAAtc                                                                  >  1:5264174/3‑70 (MQ=255)       aaTCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGt                                                                 <  1:4346016/71‑1 (MQ=255)        aTCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGCGACCGCGATTTAAtcgg                                                                <  1:3144933/71‑4 (MQ=255)         tCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGCGACCGCGAttt                                                                      <  1:4614380/64‑1 (MQ=255)         tCATGCAACATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGCGACCGCGATTTAAtcgg                                                                <  1:4086207/70‑4 (MQ=255)              cAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCt                                                          <  1:4014833/71‑1 (MQ=255)              cAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCt                                                          >  1:121453/1‑71 (MQ=255)              cAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCt                                                          >  1:1755295/1‑71 (MQ=255)               aaCATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGCGACCGCGATTTa                                                                     >  1:2398642/1‑59 (MQ=255)               aaCATCGGCGTATAGCTCTGCTGGAACTGATTCAAAAGCATATTCGCGACCGCGATTTa                                                                     >  1:2550432/1‑59 (MQ=255)                  aTCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTaa                                                                    >  1:5389881/1‑57 (MQ=255)                  aTCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTaa                                                                    >  1:4756399/1‑57 (MQ=255)                  aTCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAg                                                      >  1:3152257/1‑71 (MQ=255)                    cggcggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAAt                                                                   >  1:4727016/1‑56 (MQ=255)                    cggcggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAAt                                                                   >  1:5388259/1‑56 (MQ=255)                    cggcggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAAt                                                                   >  1:4733468/1‑56 (MQ=255)                     ggcggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCaa                                                   <  1:1726524/71‑1 (MQ=255)                     ggcggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCaa                                                   >  1:549698/1‑71 (MQ=255)                     ggcggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCaa                                                   <  1:1932043/71‑1 (MQ=255)                     ggcggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCaa                                                   >  1:5025523/1‑71 (MQ=255)                        ggATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACt                                                 <  1:1854585/70‑1 (MQ=255)                               gATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCtt                                                         <  1:1875035/55‑1 (MQ=255)                               gATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCtt                                                         <  1:1957148/55‑1 (MQ=255)                                 ttCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCt                                        >  1:5450095/1‑70 (MQ=255)                                 ttCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTg                                       <  1:2192961/71‑1 (MQ=255)                                  tctcGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGa                                      >  1:3072992/1‑71 (MQ=255)                                  tctcGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGa                                      >  1:1205416/1‑71 (MQ=255)                                   ctcGAACTACTGCAAAAACATATTCGCCAGCGCGACTTaa                                                                    >  1:1582883/1‑40 (MQ=255)                                   ctcGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCaa                                                   >  1:3476834/1‑57 (MQ=255)                                   ctcGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCaa                                                   >  1:4439828/1‑57 (MQ=255)                                      gAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTAt                                                              >  1:1217928/1‑43 (MQ=255)                                      gAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGAt                                     <  1:498423/68‑1 (MQ=255)                                      gAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATc                                    <  1:2636217/69‑1 (MQ=255)                                        actactGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATcgcc                                 >  1:4945734/1‑68 (MQ=255)                                        actactGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATcgac                                 >  1:4301254/1‑70 (MQ=255)                                        actactGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATcgac                                 <  1:27477/70‑1 (MQ=255)                                           actGCAAAAACATATTCGCCAGCGCGACTTTATGt                                                                 <  1:2813211/35‑1 (MQ=255)                                           actGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAg                             >  1:4110214/1‑71 (MQ=255)                                            ctGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAAc                                                  >  1:2780334/1‑49 (MQ=255)                                            ctGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAAc                                                  >  1:820289/1‑49 (MQ=255)                                            ctGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATc                                    <  1:1448313/63‑1 (MQ=255)                                            ctGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGaa                              >  1:723550/1‑69 (MQ=255)                                            ctGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAgg                            >  1:3767974/1‑71 (MQ=255)                                             tGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGc                                                     <  1:5630840/45‑1 (MQ=255)                                             tGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGt                                              <  1:2358735/52‑1 (MQ=255)                                              gCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATcgacga                               >  1:4396399/1‑66 (MQ=255)                                              gCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATcgacga                               >  1:5393177/1‑66 (MQ=255)                                               cAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGAt                                     >  1:748620/1‑59 (MQ=255)                                               cAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGAt                                     >  1:671609/1‑59 (MQ=255)                                               cAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGAt                                     >  1:3749686/1‑59 (MQ=255)                                               cAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGAt                                     >  1:3560665/1‑59 (MQ=255)                                               cAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATcga                                  >  1:692509/1‑62 (MQ=255)                                               cAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAggg                           <  1:4352719/69‑1 (MQ=255)                                                aaaaaCATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAg                             >  1:4778198/1‑66 (MQ=255)                                                aaaaaCATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAGGGTac                        <  1:3883655/71‑1 (MQ=255)                                                aaaaaCATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAGGGTa                         <  1:3334884/70‑1 (MQ=255)                                                aaaaaCATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAGGGTa                         <  1:3088165/70‑1 (MQ=255)                                                aaaaaCATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCAACGAAg                             >  1:3339152/1‑66 (MQ=255)                                                  aaaCATATTCGCCAGCGCGACTTAATGTTATTGCtt                                                         <  1:1658124/36‑1 (MQ=255)                                                  aaaCATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATcg                                   <  1:279077/58‑1 (MQ=255)                                                  aaaCATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAGGGTacac                      >  1:4318894/1‑71 (MQ=255)                                                    aCATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAGGGTacac                      >  1:2959748/1‑69 (MQ=255)                                                    aCATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAGGGTacac                      >  1:542714/1‑69 (MQ=255)                                                    aCATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAGGGTacac                      >  1:1876175/1‑69 (MQ=255)                                                    aCATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAGGGTACACTa                    <  1:3289267/71‑1 (MQ=255)                                                       tatTCGCCAGCGCGACTTAATGTTATTGCTTGAGc                                                     <  1:1573545/35‑1 (MQ=255)                                                       tatTCGCCAGCGCGACTTAATGTTATTGCTTGAGc                                                     <  1:1829889/35‑1 (MQ=255)                                                       tatTCGCCAGCGCGACTTAATGTTATTGCTTGAGc                                                     <  1:927884/35‑1 (MQ=255)                                                       tatTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAGGGTACACTAGCg                 <  1:1865938/71‑1 (MQ=255)                                                         ttCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAGGGTACACTAGc                  >  1:4745196/1‑68 (MQ=255)                                                         ttCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAGGGTACACTAGc                  >  1:3016066/1‑68 (MQ=255)                                                         ttCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAGGGTACACTAGCgg                <  1:2048286/70‑1 (MQ=255)                                                          tCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAGGGTACACTAGCgg                >  1:3781331/1‑69 (MQ=255)                                                           cGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAGGGTACACTAGCGGAAg             >  1:1088716/1‑71 (MQ=255)                                                           cGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAGGGTACACTAGCGGAAg             >  1:631586/1‑71 (MQ=255)                                                            gCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAg                             >  1:4285262/1‑54 (MQ=255)                                                            gCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAg                             >  1:661355/1‑54 (MQ=255)                                                            gCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAGGGTACACTAGCGGAAGt            <  1:4878921/71‑1 (MQ=255)                                                            gCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAGGGTACACTAGCGGAAGt            <  1:2896582/71‑1 (MQ=255)                                                              cAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAGGGTACACTAGCGGAAGTCa          <  1:5001049/71‑1 (MQ=255)                                                                gcgcGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAGGGTa                         <  1:2587922/54‑1 (MQ=255)                                                                gcgcGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAGGGTa                         <  1:2100752/54‑1 (MQ=255)                                                                gcgcGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAGGGTACACTAGCgg                <  1:3984407/63‑1 (MQ=255)                                                                gcgcGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAGGGTACACTAGCgg                <  1:3580560/63‑1 (MQ=255)                                                                gcgcGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAGGGTACACTAGCgg                <  1:3554239/63‑1 (MQ=255)                                                                gcgcGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAGGGTACACTAGCgg                <  1:1524346/63‑1 (MQ=255)                                                                gcgcGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAGGGTACACTAGCGGAAGTCagt        <  1:1092622/71‑1 (MQ=255)                                                                gcgcGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAGGGTACACTAGCGGAAGTCagt        <  1:4992125/71‑1 (MQ=255)                                                                   cgACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAGGGTACACTAGCGGAAGTCagtta      <  1:1388029/70‑1 (MQ=255)                                                                   cgACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAGGGTACACTAGCGGAAGTCagtta      <  1:946002/70‑1 (MQ=255)                                                                     aCTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATc                                    <  1:551970/38‑1 (MQ=255)                                                                     aCTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATc                                    <  1:5042420/38‑1 (MQ=255)                                                                     aCTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATc                                    <  1:827682/38‑1 (MQ=255)                                                                     aCTTAATGTTATTGCTTGAGCAACTGGGCACGCTGATc                                    <  1:2578643/38‑1 (MQ=255)                                                                      cTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAGGGTACACTAGCGGAAGTCAGTTAGTTg  <  1:999182/71‑1 (MQ=255)                                                                      |                                                                       TGACGAAATCATGCAACATCGGCGGATTGCGATTCTCGAACTACTGCAAAAACATATTCGCCAGCGCGACTTAATGTTATTGCTTGAGCAACTGGTCACGCTGATCGACGAAGGGTACACTAGCGGAAGTCAGTTAGTTG  >  Exported/132196‑132335

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑