Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 387,150 T→C 11.2% D24D (GAT→GAC ybdD → conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported387,1500TC11.2% 325.9 / 25.3 125D24D (GAT→GACybdDconserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (66/45);  new base C (8/6);  total (74/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01

Only 100 of 131 total aligned reads displayed.

TGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATGACCTACGAAGAGTTTT  >  Exported/387080‑387220
                                                                      |                                                                      
  tttGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTa                                                                      <  1:1122434/71‑1 (MQ=255)
   ttGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTAc                                                                     >  1:2445598/1‑71 (MQ=255)
   ttGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTAc                                                                     >  1:1661989/1‑71 (MQ=255)
    tGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTAc                                                                     >  1:2071149/1‑70 (MQ=255)
    tGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTAc                                                                     >  1:1729895/1‑70 (MQ=255)
    tGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTAc                                                                     >  1:2450142/1‑70 (MQ=255)
         cACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACaa                                                                >  1:2493443/1‑70 (MQ=255)
         cACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAAc                                                               >  1:4476256/1‑71 (MQ=255)
          aCTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAAc                                                               >  1:305563/1‑70 (MQ=255)
                aaaaGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGAt                                                                        >  1:5565807/1‑55 (MQ=255)
                aaaaGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGAt                                                                        >  1:5572510/1‑55 (MQ=255)
                   aGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGaa                                                      >  1:3932333/1‑70 (MQ=255)
                   aGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAAc                                                     <  1:2296528/71‑1 (MQ=255)
                     ccGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACa                                                    >  1:4169278/1‑70 (MQ=255)
                      cGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACaa                                                                <  1:4582039/57‑1 (MQ=255)
                      aaaaaaaTATCTCGGCCAGGCGGCGAAGATGTTGATTGGCATTCCAGACTACGACAACTACGTCGAGCata                                                  >  1:1375512/4‑71 (MQ=255)
                        gAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACt                                                              >  1:2710601/1‑57 (MQ=255)
                        gAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACt                                                              >  1:3960817/1‑57 (MQ=255)
                        gAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACt                                                              >  1:1563660/1‑57 (MQ=255)
                        gAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACt                                                              >  1:4746026/1‑57 (MQ=255)
                        aaaaaTATCTCGGCCAGGCGGCGAAGATGTTGATTGGCATTCCAGACTACGACAACTACGTCGAGCATATg                                                <  1:2994722/70‑1 (MQ=255)
                        aaaaaTATCTCGGCCAGGCGGCGAAGATGTTGATTGGCATTCCAGACTACGACAACTACGTCGAGCATATg                                                <  1:784947/70‑1 (MQ=255)
                         aaaaTATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGAt                                                                        >  1:1798256/1‑46 (MQ=255)
                         aaaaTATCTCGGCCAGGCGGCGAAGATGTTGATTGGCATTCCAGACTAc                                                                     >  1:307246/1‑49 (MQ=255)
                         aaaaTATCTCGGCCAGGCGGCGAAGATGTTGATTGGCATTCCAGACTACGACAACTACGTCGAGCATATg                                                >  1:2617577/1‑70 (MQ=255)
                          aaaTATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCg                                              >  1:405943/1‑71 (MQ=255)
                          aaaTATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCg                                              >  1:331928/1‑71 (MQ=255)
                          aaaTATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCg                                              <  1:3826447/71‑1 (MQ=255)
                            atatTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCggg                                            >  1:5142009/1‑71 (MQ=255)
                              atTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGt                                           >  1:3267710/1‑70 (MQ=255)
                              atTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGt                                           >  1:3907523/1‑70 (MQ=255)
                              atTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGt                                           >  1:1579984/1‑70 (MQ=255)
                               tttAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTa                                         <  1:3716080/71‑1 (MQ=255)
                                ttAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTaa                                        >  1:2608251/1‑71 (MQ=255)
                                 tAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAAc                                       >  1:4330098/1‑71 (MQ=255)
                                  aGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAAc                                       <  1:342600/70‑1 (MQ=255)
                                        ggcggcGAAGCTGATGATTGGTATGCCTGATTACGACAACTa                                                             >  1:1023549/1‑42 (MQ=255)
                                           ggcgAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACata                                                  >  1:4862206/1‑50 (MQ=255)
                                           ggcgAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACata                                                  >  1:2670197/1‑50 (MQ=255)
                                           ggcgAAGATGTTGATTGGCATTCCAGACTACGACAACTACGTCGAGCATATgaa                                              >  1:3133247/1‑52 (MQ=255)
                                            gcgAAGTTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTaaac                                      <  1:4942000/61‑3 (MQ=255)
                                            gcgAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAAcc                                      <  1:4683993/61‑1 (MQ=255)
                                            gcgAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCa                            <  1:4922145/71‑1 (MQ=255)
                                             cgAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTa                                         <  1:2599167/57‑1 (MQ=255)
                                              gAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAAc                                                     <  1:2855109/44‑1 (MQ=255)
                                              gAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAAc                                                     <  1:1450240/44‑1 (MQ=255)
                                              gAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAAc                                                     <  1:3675457/44‑1 (MQ=255)
                                              gAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCaaa                          >  1:5554143/1‑71 (MQ=255)
                                               aaGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAAc                         <  1:1930727/71‑1 (MQ=255)
                                                aGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGaa                                                      >  1:4688802/1‑41 (MQ=255)
                                                aGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACat                                                   >  1:4705646/1‑44 (MQ=255)
                                                aGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACat                                                   >  1:3678828/1‑44 (MQ=255)
                                                 gCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCgg                                             >  1:3400112/1‑49 (MQ=255)
                                                 gCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCgg                                             >  1:5383963/1‑49 (MQ=255)
                                                 gCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCgg                                             >  1:4724351/1‑49 (MQ=255)
                                                 gCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCgg                                             >  1:141617/1‑49 (MQ=255)
                                                 gCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCgg                                             >  1:4655295/1‑49 (MQ=255)
                                                  cTGATGATTGGTATGCCTGATTACGACAACTATGTCGa                                                       <  1:3402980/38‑1 (MQ=255)
                                                  cTGATGATTGGTATGCCTGATTACGACAACTATGTCGa                                                       <  1:1829094/38‑1 (MQ=255)
                                                  cTGATGATTGGTATGCCTGATTACGACAACTATGTCGa                                                       <  1:3186493/38‑1 (MQ=255)
                                                  cTGATGATTGGTATGCCTGATTACGACAACTATGTCGa                                                       <  1:3094796/38‑1 (MQ=255)
                                                  cTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGcc                      <  1:4705111/71‑1 (MQ=255)
                                                  cTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGcc                      <  1:4857755/71‑1 (MQ=255)
                                                  cTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGc                       >  1:1988167/1‑70 (MQ=255)
                                                   tgatgaTTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCaaa                          >  1:4794735/1‑66 (MQ=255)
                                                   tgatgaTTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCa                            <  1:2149469/64‑1 (MQ=255)
                                                    gatgatTGGTATGCCTGATTACGACAACTATGTCGAAc                                                     >  1:504197/1‑38 (MQ=255)
                                                    gatgatTGGTATGCCTGATTACGACAACTATGTCGAAc                                                     >  1:1959478/1‑38 (MQ=255)
                                                    gatgatTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTa                                         >  1:938213/1‑50 (MQ=255)
                                                    gatgatTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCg                     <  1:2939755/70‑1 (MQ=255)
                                                    gatgatTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGa                    >  1:4499753/1‑71 (MQ=255)
                                                     ttgatTGGCATTCCAGACTACGACAACTACGTCGAGCATATg                                                <  1:2812804/41‑1 (MQ=255)
                                                     ttgatTGGCATTCCAGACTACGACAACTACGTCGAGCATATg                                                <  1:2616689/41‑1 (MQ=255)
                                                     atgatTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAAc                                       >  1:1637707/1‑51 (MQ=255)
                                                     atgatTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAAc                                       >  1:2251007/1‑51 (MQ=255)
                                                     atgatTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCaaa                          >  1:3227089/1‑64 (MQ=255)
                                                     atgatTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCaaa                          >  1:790725/1‑64 (MQ=255)
                                                     atgatTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGAt                   <  1:2675114/71‑1 (MQ=255)
                                                      tgatTGGTATGCCTGATTACGACAACTATGTCGAACata                                                  >  1:1283650/1‑39 (MQ=255)
                                                      tgatTGGTATGCCTGATTACGACAACTATGTCGAACata                                                  >  1:5366212/1‑39 (MQ=255)
                                                      tgatTGGCATTCCAGACTACGACAACTACGTCGAGCATATgaa                                              >  1:1488582/1‑41 (MQ=255)
                                                       gatTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCaaa                          >  1:906862/1‑62 (MQ=255)
                                                       gatTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCaaa                          >  1:1087035/1‑62 (MQ=255)
                                                            gTATGCCTGATTACGACAACTATGTCGATCATATGCGGGTTAACCATCCCGATCAAACGCCGATGAcc               <  1:3956184/68‑1 (MQ=255)
                                                            gTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATGAcc               <  1:4742001/68‑1 (MQ=255)
                                                             tATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATGACCTACg           <  1:2161725/71‑1 (MQ=255)
                                                              aTGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATGAcc               <  1:4525697/66‑1 (MQ=255)
                                                               tGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATc                             <  1:2533314/51‑1 (MQ=255)
                                                               tGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATc                             <  1:3262400/51‑1 (MQ=255)
                                                               tGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATg                  <  1:962539/62‑1 (MQ=255)
                                                               tGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATg                  <  1:4388754/62‑1 (MQ=255)
                                                                gCCTGATTACGACAACTATGTCGAACATATGCGGGTTAAcc                                      <  1:477154/41‑1 (MQ=255)
                                                                gCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCTTCCCGATCa                            <  1:5054868/51‑1 (MQ=255)
                                                                   tGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCg                                <  1:2534417/44‑1 (MQ=255)
                                                                   tGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATg                  <  1:1671054/58‑1 (MQ=255)
                                                                     aTTACGACAACTATGTCGAACATATGCGGGTTAACCATccc                                 >  1:214245/1‑41 (MQ=255)
                                                                     aTTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGAt                              >  1:2376903/1‑44 (MQ=255)
                                                                     aTTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGcc                      <  1:600208/52‑1 (MQ=255)
                                                                     aTTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATGACCTACGAAGAGttt   >  1:2478839/1‑71 (MQ=255)
                                                                     aTTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATGACCTACGAAGAGt     <  1:3980287/69‑1 (MQ=255)
                                                                      |                                                                      
TGTTTGATTCACTGGCAAAAGCCGGAAAATATTTAGGTCAGGCGGCGAAGCTGATGATTGGTATGCCTGATTACGACAACTATGTCGAACATATGCGGGTTAACCATCCCGATCAAACGCCGATGACCTACGAAGAGTTTT  >  Exported/387080‑387220

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: