Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 453,894 A→C 6.9% V397V (GTT→GTG ybgH ← predicted transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported453,8940AC 6.9% 179.3 / 7.1 72V397V (GTT→GTGybgHpredicted transporter
Reads supporting (aligned to +/- strand):  ref base A (39/28);  new base C (3/2);  total (42/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.42e-01

TGTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGAGAAG  >  Exported/453830‑453961
                                                                |                                                                   
tGTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCggg                                                               <  1:3208593/71‑1 (MQ=255)
tGTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCggg                                                               <  1:457871/71‑1 (MQ=255)
tGTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCggg                                                               >  1:3191217/1‑71 (MQ=255)
tGTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCggg                                                               <  1:1996475/71‑1 (MQ=255)
  tAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTc                                                             >  1:265231/1‑71 (MQ=255)
  tAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTc                                                             >  1:1880966/1‑71 (MQ=255)
      tGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGAt                                                          >  1:4846603/1‑70 (MQ=255)
      tGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATa                                                         <  1:1441290/71‑1 (MQ=255)
      tGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATa                                                         <  1:2435023/71‑1 (MQ=255)
       gCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATa                                                         <  1:2931615/70‑1 (MQ=255)
        ccGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATaaa                                                       <  1:4444835/71‑1 (MQ=255)
           gTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAg                                                    >  1:4184714/1‑71 (MQ=255)
              aaTACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGtt                                                  <  1:1686551/70‑1 (MQ=255)
              aaTACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGtt                                                  <  1:810175/70‑1 (MQ=255)
                  cGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGc                                              >  1:842821/1‑70 (MQ=255)
                    ccGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCaaa                                           <  1:5291374/71‑1 (MQ=255)
                         cacaCCGGGGATTTCAATGCGCGTAATTTGCGACATGGCa                                                                     <  1:3103625/40‑1 (MQ=255)
                         cacaCCGGGGATTTCAATGCGCGTAATTTGCGACATGGCa                                                                     <  1:3417779/40‑1 (MQ=255)
                         cacaCCGGGGATTTCAATGCGCGTAATTTGCGACATGGCa                                                                     <  1:833701/40‑1 (MQ=255)
                         cacaCCGGGGATTTCAATGCGCGTAATTAGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGccc                                       >  1:2239834/1‑70 (MQ=255)
                          acacCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCAt                                     >  1:4220582/1‑71 (MQ=255)
                           cacCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAAc                                                      <  1:5337443/53‑1 (MQ=255)
                           cacCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAAc                                                      <  1:1644636/53‑1 (MQ=255)
                           cacCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAAc                                                      <  1:158017/53‑1 (MQ=255)
                           cacCGGAGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAACGCCCAt                                     >  1:5900/1‑70 (MQ=255)
                            acCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAAc                                                                   >  1:3661368/1‑39 (MQ=255)
                            acCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAAc                                                                   >  1:3193616/1‑39 (MQ=255)
                             ccGGGGATTTCAATGCGCGTAATTTGCGACATGGCAAc                                                                   >  1:3610001/1‑38 (MQ=255)
                             ccGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATa                                                         >  1:2512915/1‑48 (MQ=255)
                              cGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCAcc                                 >  1:2404714/1‑71 (MQ=255)
                              cGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCAcc                                 >  1:2310920/1‑71 (MQ=255)
                              cGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCAcc                                 >  1:4167049/1‑71 (MQ=255)
                              cGGGGATTTCAATGCGCCTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCaaa                                           <  1:345052/61‑1 (MQ=255)
                               ggggATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCg                                >  1:11578/1‑71 (MQ=255)
                                gggATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATaaa                                                       >  1:5340545/1‑47 (MQ=255)
                                gggATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATaaa                                                       >  1:3139831/1‑47 (MQ=255)
                                  gATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAg                                          >  1:4070724/1‑58 (MQ=255)
                                   aTTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGcc                              <  1:957240/69‑1 (MQ=255)
                                   aTTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAg                            >  1:4179011/1‑71 (MQ=255)
                                    tttCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAgg                           >  1:4826648/1‑71 (MQ=255)
                                    tttCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAgg                           >  1:2737780/1‑71 (MQ=255)
                                     ttCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCAt                                     <  1:3018891/60‑1 (MQ=255)
                                       cAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATaaa                                                       >  1:1095301/1‑40 (MQ=255)
                                          tGCGCGTAATTTGCGACATTGCAACCGGGTCGATa                                                         <  1:5176365/35‑1 (MQ=255)
                                          tGCGCGTAATTTGCGACATGGCAACCGGGTCGATa                                                         <  1:3526013/35‑1 (MQ=255)
                                          tGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTaa                      >  1:196897/1‑70 (MQ=255)
                                          tGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAAt                     <  1:492960/71‑1 (MQ=255)
                                           gcgcGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCa                             >  1:3699689/1‑62 (MQ=255)
                                            cgcgTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGccc                                       >  1:3348645/1‑51 (MQ=255)
                                               gtgaTTTGCGCAATCGCCACCGGGTCAATAAAGAGTTCGGCAAAGCCCATTAGCGCCAGCCCGGATATCAt                >  1:4427525/4‑71 (MQ=255)
                                                tAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCt                        <  1:3508119/62‑1 (MQ=255)
                                                tAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCt                        <  1:3640485/62‑1 (MQ=255)
                                                 aaTTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCaaa                                           >  1:3765145/1‑42 (MQ=255)
                                                  aTTTGCGCAATCGCCACCGGGTCAATAAAGAGTTCGGCAAAGCCCa                                      >  1:3732216/1‑46 (MQ=255)
                                                  aTTTGCGCAATCGCCACCGGGTCAATAAAGAGTTCGGCAAAGCCCa                                      >  1:5094101/1‑46 (MQ=255)
                                                  aTTTGCGCAATCGCCACCGGGTCAATAAAGAGTTCGGCAAAGCCCATTAGCGCCAGCCCGGATAtcatcac             <  1:4273078/71‑2 (MQ=255)
                                                  aTTTGCGCAATCGCCACCGGGTCAATAAAGAGTTCGGCAAAGCCCATTAGCGCCAGCCCGGATAtcatcac             <  1:2031314/71‑2 (MQ=255)
                                                  aTTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAAt                     >  1:2499133/1‑63 (MQ=255)
                                                   tttGCGACATGGCAACCGGGTCGATAAACAGTTCCGc                                              <  1:4969394/37‑1 (MQ=255)
                                                   tttGCGACATGGCAACCGGGTCGATAAACAGTTCCGc                                              <  1:3443255/37‑1 (MQ=255)
                                                   tttGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTaa                      >  1:2579874/1‑61 (MQ=255)
                                                   tttGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTaa                      >  1:937381/1‑61 (MQ=255)
                                                   tttGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTaa                      >  1:4457961/1‑61 (MQ=255)
                                                   tttGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTaa                      >  1:315219/1‑61 (MQ=255)
                                                   tttGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTaa                      >  1:655023/1‑61 (MQ=255)
                                                     tGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCa                                   >  1:93137/1‑46 (MQ=255)
                                                     tGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGTCCTAATACCATCAGTgg          >  1:199680/1‑71 (MQ=255)
                                                     tGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTgg          >  1:542266/1‑71 (MQ=255)
                                                        gACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCag       <  1:63939/71‑1 (MQ=255)
                                                         aCATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCa        >  1:2817230/1‑69 (MQ=255)
                                                            tGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGagaa   <  1:496719/71‑1 (MQ=255)
                                                            tGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGagaa   <  1:1431740/71‑1 (MQ=255)
                                                            tGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGagaa   <  1:4152717/71‑1 (MQ=255)
                                                              gCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGagaag  <  1:2664782/70‑1 (MQ=255)
                                                              gCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGagaa   >  1:2964123/1‑69 (MQ=255)
                                                                aaCCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCagag     <  1:970742/65‑1 (MQ=255)
                                                                |                                                                   
TGTAGATGCCGGTTAATACGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGAGAAG  >  Exported/453830‑453961

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: