Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	453,915	C→G	7.8%	A390A (GCG→GCC)	ybgH ←	predicted transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	453,915	0	C	G	7.8%	188.6 / 5.1	64	A390A (GCG→GCC)	ybgH	predicted transporter
Reads supporting (aligned to +/- strand):  ref base C (42/17);  new base G (3/2);  total (45/19)
Fisher's exact test for biased strand distribution p-value = 6.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.26e-01

CGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGAGAAGAGTGACCATACATTGCGGACCAG  >  Exported/453848‑453984                                                                    |                                                                      cGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGc                                                                     >  1:842821/1‑70 (MQ=255)   ccGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCaaa                                                                  <  1:5291374/71‑1 (MQ=255)        cacaCCGGGGATTTCAATGCGCGTAATTAGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGccc                                                              >  1:2239834/1‑70 (MQ=255)         acacCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCAt                                                            >  1:4220582/1‑71 (MQ=255)          cacCGGAGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAACGCCCAt                                                            >  1:5900/1‑70 (MQ=255)             cGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCAcc                                                        >  1:4167049/1‑71 (MQ=255)             cGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCAcc                                                        >  1:2310920/1‑71 (MQ=255)             cGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCAcc                                                        >  1:2404714/1‑71 (MQ=255)             cGGGGATTTCAATGCGCCTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCaaa                                                                  <  1:345052/61‑1 (MQ=255)              ggggATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCg                                                       >  1:11578/1‑71 (MQ=255)                 gATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAg                                                                 >  1:4070724/1‑58 (MQ=255)                  aTTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGcc                                                     <  1:957240/69‑1 (MQ=255)                  aTTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAg                                                   >  1:4179011/1‑71 (MQ=255)                   tttCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAgg                                                  >  1:4826648/1‑71 (MQ=255)                   tttCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAgg                                                  >  1:2737780/1‑71 (MQ=255)                    ttCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCAt                                                            <  1:3018891/60‑1 (MQ=255)                         tGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTaa                                             >  1:196897/1‑70 (MQ=255)                         tGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAAt                                            <  1:492960/71‑1 (MQ=255)                          gcgcGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCa                                                    >  1:3699689/1‑62 (MQ=255)                           cgcgTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGccc                                                              >  1:3348645/1‑51 (MQ=255)                              gtgaTTTGCGCAATCGCCACCGGGTCAATAAAGAGTTCGGCAAAGCCCATTAGCGCCAGCCCGGATATCAt                                       >  1:4427525/4‑71 (MQ=255)                               tAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCt                                               <  1:3640485/62‑1 (MQ=255)                               tAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCt                                               <  1:3508119/62‑1 (MQ=255)                                aaTTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCaaa                                                                  >  1:3765145/1‑42 (MQ=255)                                 aTTTGCGCAATCGCCACCGGGTCAATAAAGAGTTCGGCAAAGCCCa                                                             >  1:5094101/1‑46 (MQ=255)                                 aTTTGCGCAATCGCCACCGGGTCAATAAAGAGTTCGGCAAAGCCCa                                                             >  1:3732216/1‑46 (MQ=255)                                 aTTTGCGCAATCGCCACCGGGTCAATAAAGAGTTCGGCAAAGCCCATTAGCGCCAGCCCGGATAtcatcac                                    <  1:4273078/71‑2 (MQ=255)                                 aTTTGCGCAATCGCCACCGGGTCAATAAAGAGTTCGGCAAAGCCCATTAGCGCCAGCCCGGATAtcatcac                                    <  1:2031314/71‑2 (MQ=255)                                 aTTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAAt                                            >  1:2499133/1‑63 (MQ=255)                                  tttGCGACATGGCAACCGGGTCGATAAACAGTTCCGc                                                                     <  1:3443255/37‑1 (MQ=255)                                  tttGCGACATGGCAACCGGGTCGATAAACAGTTCCGc                                                                     <  1:4969394/37‑1 (MQ=255)                                  tttGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTaa                                             >  1:937381/1‑61 (MQ=255)                                  tttGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTaa                                             >  1:4457961/1‑61 (MQ=255)                                  tttGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTaa                                             >  1:2579874/1‑61 (MQ=255)                                  tttGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTaa                                             >  1:655023/1‑61 (MQ=255)                                  tttGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTaa                                             >  1:315219/1‑61 (MQ=255)                                    tGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCa                                                          >  1:93137/1‑46 (MQ=255)                                    tGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGTCCTAATACCATCAGTgg                                 >  1:199680/1‑71 (MQ=255)                                    tGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTgg                                 >  1:542266/1‑71 (MQ=255)                                       gACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCag                              <  1:63939/71‑1 (MQ=255)                                        aCATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCa                               >  1:2817230/1‑69 (MQ=255)                                           tGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGagaa                          <  1:4152717/71‑1 (MQ=255)                                           tGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGagaa                          <  1:1431740/71‑1 (MQ=255)                                           tGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGagaa                          <  1:496719/71‑1 (MQ=255)                                             gCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGagaag                         <  1:2664782/70‑1 (MQ=255)                                             gCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGagaa                          >  1:2964123/1‑69 (MQ=255)                                               aaCCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCagag                            <  1:970742/65‑1 (MQ=255)                                                 ccGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGAGAAGAGTGa                    <  1:3441930/71‑1 (MQ=255)                                                  cGGTTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGAGAAGAGTGAc                   >  1:3428610/1‑71 (MQ=255)                                                  cGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATAcc                                         >  1:4291826/1‑49 (MQ=255)                                                  cGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGagaaga                        >  1:3739576/1‑66 (MQ=255)                                                  cGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGagaaga                        >  1:3224927/1‑66 (MQ=255)                                                  cGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGagaaga                        >  1:4803228/1‑66 (MQ=255)                                                  cGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGAGAAGAGTGAc                   >  1:1983394/1‑71 (MQ=255)                                                  cGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGAGAAGAGTGAc                   >  1:2630935/1‑71 (MQ=255)                                                    ggTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCa                                     >  1:4381831/1‑51 (MQ=255)                                                           aaaCAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGAGAAGAGTGACCATACATTg          >  1:3501221/1‑71 (MQ=255)                                                            aaCAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGAGAAGAGTGACCATACATTg          <  1:4135773/70‑1 (MQ=255)                                                                 ttCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGAGAAGAGTGACCATACATTg          >  1:5547377/1‑65 (MQ=255)                                                                  tCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCa                                     >  1:5467602/1‑37 (MQ=255)                                                                  tCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCa                                     >  1:2046932/1‑37 (MQ=255)                                                                  tCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCa                                     >  1:5289835/1‑37 (MQ=255)                                                                  tCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCa                                     >  1:1122304/1‑37 (MQ=255)                                                                  tCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGAGAAGAGTGACCATACATTGCGGAc     >  1:808467/1‑69 (MQ=255)                                                                   ccGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGAGAAGAGTGACCATACa             >  1:1067316/1‑60 (MQ=255)                                                                    cGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGAGAAGAGTGACCATACATTGCGGACCAg  <  1:5527685/70‑1 (MQ=255)                                                                    |                                                                      CGCCGGTCACACCGGGGATTTCAATGCGCGTAATTTGCGACATGGCAACCGGGTCGATAAACAGTTCCGCAAAGCCCATCACCGCCAGGCCTAATACCATCAGTGGCAGAGAAGAGTGACCATACATTGCGGACCAG  >  Exported/453848‑453984

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑