Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 454,710 G→A 14.9% L125L (CTC→CTT ybgH ← predicted transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported454,7100GA14.9% 276.3 / 32.2 114L125L (CTC→CTTybgHpredicted transporter
Reads supporting (aligned to +/- strand):  ref base G (49/48);  new base A (9/8);  total (58/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 116 total aligned reads displayed.

CGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGG  >  Exported/454640‑454779
                                                                      |                                                                     
cGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCg                                                                       <  1:5280789/71‑1 (MQ=255)
   tACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAg                                                                     >  1:261339/1‑70 (MQ=255)
    gcccGCAGCATACAGCAGCGAAAAACCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGc                                                                    >  1:1873060/2‑70 (MQ=255)
     cccGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCa                                                                   <  1:2095070/70‑1 (MQ=255)
     cccGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCa                                                                   <  1:3502401/70‑1 (MQ=255)
     cccGCAGCATACAGCAGCGAAAAACCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCa                                                                   <  1:4836805/70‑1 (MQ=255)
          agcATACAGCAGCGAAAAACCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAAc                                                                <  1:5091729/67‑1 (MQ=255)
          agcATACAGCAGCGAAAAACCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAg                                                              >  1:4744271/2‑70 (MQ=255)
             aTACAGCAGCGAAAAACCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCtga                                                          >  1:49635/1‑69 (MQ=255)
              tACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAg                                                                     <  1:5309415/59‑1 (MQ=255)
              tACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAg                                                                     <  1:3852371/59‑1 (MQ=255)
              tACAGCAGCGAAAAACCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCtgat                                                         >  1:3753874/1‑68 (MQ=255)
              tACAGCAGCGAAAAACCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCtgat                                                         >  1:296372/1‑68 (MQ=255)
                cATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCa                                                                   >  1:1126806/1‑59 (MQ=255)
                cATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGt                                                       >  1:4004165/1‑71 (MQ=255)
                 aTCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGtt                                                      <  1:3866004/71‑1 (MQ=255)
                  tCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCa                                                                   >  1:5491708/1‑57 (MQ=255)
                   cAGCGAAAAACCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCTGATGttt                                                     >  1:4326694/1‑69 (MQ=255)
                    aGCGAAAAACCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCTGATGTTTGa                                                   <  1:1171842/71‑1 (MQ=255)
                       gagaAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGAtt                                                 >  1:4574586/1‑70 (MQ=255)
                       gagaAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGAtt                                                 >  1:2078218/1‑70 (MQ=255)
                       gagaAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGAtt                                                 >  1:4849648/1‑70 (MQ=255)
                       gaaaaaCCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCTGATGTTTGAttt                                                <  1:1086908/68‑1 (MQ=255)
                        agaAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTa                                               <  1:3817507/71‑1 (MQ=255)
                        agaAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTa                                               <  1:1105808/71‑1 (MQ=255)
                        agaAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTa                                               <  1:742179/71‑1 (MQ=255)
                        agaAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTa                                               <  1:1109709/71‑1 (MQ=255)
                        agaAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTa                                               <  1:2163150/71‑1 (MQ=255)
                        aaaaaCCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCTGATGTTTGAtttg                                               <  1:1687386/69‑2 (MQ=255)
                         aaaaCCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCTGATGTTTGAtttga                                              <  1:1109761/70‑3 (MQ=255)
                         aaaaCCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCTGATGTTTGAtttga                                              <  1:3393136/70‑3 (MQ=255)
                         aaaaCCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCTGATGTTTGAtttg                                               >  1:674426/2‑69 (MQ=255)
                          aaaCCGCCATCACGTCGATGATCGTTCTCGTCGTAGAGCTCGCCAAGCAAACAGCtga                                                          >  1:1477802/1‑56 (MQ=255)
                          aaaCCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTa                                               >  1:2577328/1‑69 (MQ=255)
                           aaCCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTaaa                                             <  1:1567786/70‑1 (MQ=255)
                           aaCCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAAc                                            <  1:5510105/71‑1 (MQ=255)
                           aaCCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAAc                                            <  1:5627258/71‑1 (MQ=255)
                            aCCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTaaa                                             >  1:3771252/1‑69 (MQ=255)
                            aCCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTaaa                                             >  1:2965054/1‑69 (MQ=255)
                               gCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGAttt                                                >  1:1372152/1‑63 (MQ=255)
                                ccATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTAc                                                         >  1:5221943/1‑53 (MQ=255)
                                ccATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTAc                                                         >  1:1110962/1‑53 (MQ=255)
                                ccATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGAtt                                                 <  1:3610407/61‑1 (MQ=255)
                                 cATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAg                                                                     >  1:3695262/1‑40 (MQ=255)
                                 cATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTaaa                                             >  1:696834/1‑64 (MQ=255)
                                 cATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTaaa                                             >  1:2182490/1‑64 (MQ=255)
                                   tCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGAc                                                                <  1:4230463/43‑1 (MQ=255)
                                   tCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGtt                                                      <  1:4060548/53‑1 (MQ=255)
                                   tCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGtt                                                      <  1:3970061/53‑1 (MQ=255)
                                   tCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTa                                               <  1:3263689/60‑1 (MQ=255)
                                    cACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGAc                                                                <  1:3938897/42‑1 (MQ=255)
                                     acgacgCGGATCGGTTGGCTCATACAGCTCGCCGAg                                                                     <  1:2997829/36‑1 (MQ=255)
                                     acgacgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAg                                                              >  1:2915267/1‑43 (MQ=255)
                                     acgacgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCt                                                            <  1:321950/45‑1 (MQ=255)
                                     acgacgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCt                                                            <  1:3996830/45‑1 (MQ=255)
                                     acgacgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCt                                                            <  1:2100564/45‑1 (MQ=255)
                                     acgacgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGc                                  >  1:3275336/1‑71 (MQ=255)
                                       gacgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCg                                >  1:3473341/1‑71 (MQ=255)
                                       gacgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCg                                <  1:1205343/71‑1 (MQ=255)
                                        acgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGt                                                       <  1:2854758/47‑1 (MQ=255)
                                        acgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGt                                                       <  1:3498253/47‑1 (MQ=255)
                                        acgCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTaa                                              <  1:2667699/56‑1 (MQ=255)
                                             gATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTaaa                                             >  1:2236478/1‑52 (MQ=255)
                                             gATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTa                                               <  1:5402684/50‑1 (MQ=255)
                                             gATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACg                          <  1:1842496/71‑1 (MQ=255)
                                               tCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGAt                        <  1:4243344/71‑1 (MQ=255)
                                                cGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGAt                                                  >  1:692470/1‑44 (MQ=255)
                                                 ggTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATa                       <  1:3777455/70‑1 (MQ=255)
                                                     ggCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGc                  >  1:3050148/1‑71 (MQ=255)
                                                     ggCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGc                  >  1:5072854/1‑71 (MQ=255)
                                                      gCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGc                  <  1:4311849/70‑1 (MQ=255)
                                                        tCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGAc                           >  1:5183545/1‑59 (MQ=255)
                                                         cATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAgg              >  1:584049/1‑71 (MQ=255)
                                                         cATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAgg              >  1:5188323/1‑71 (MQ=255)
                                                          aTACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTaaa                                             >  1:4143060/1‑39 (MQ=255)
                                                          aTACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTaa                                              <  1:5125345/38‑1 (MQ=255)
                                                          aTACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATa                       <  1:418905/61‑1 (MQ=255)
                                                           tACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTa                                               <  1:2368459/36‑1 (MQ=255)
                                                           tACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTa                                               <  1:5052421/36‑1 (MQ=255)
                                                           tACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTa                                               <  1:2009076/36‑1 (MQ=255)
                                                           tACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCg                                <  1:4566114/51‑1 (MQ=255)
                                                           tACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGa            <  1:947705/71‑1 (MQ=255)
                                                            aCAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATa                       >  1:3571251/1‑59 (MQ=255)
                                                            aCAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGa            >  1:635432/1‑70 (MQ=255)
                                                             cAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAAc                                            >  1:3553263/1‑37 (MQ=255)
                                                             cAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCAt                                     >  1:1600839/1‑44 (MQ=255)
                                                             cAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCAt                                     >  1:5172477/1‑44 (MQ=255)
                                                             cAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCAt                                     >  1:3776662/1‑44 (MQ=255)
                                                             cAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATc                    >  1:2676194/1‑61 (MQ=255)
                                                             cAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAg               >  1:3311335/1‑66 (MQ=255)
                                                             cAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGa            >  1:1643201/1‑69 (MQ=255)
                                                             cAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGa            >  1:5314031/1‑69 (MQ=255)
                                                               gCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGa        <  1:908907/71‑1 (MQ=255)
                                                               gCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGa        <  1:1723237/71‑1 (MQ=255)
                                                                   gCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAAt                     >  1:493776/1‑54 (MQ=255)
                                                                   gCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATag     <  1:2707101/70‑1 (MQ=255)
                                                                   gCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATa      >  1:2761581/1‑69 (MQ=255)
                                                                   gCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATa      <  1:4056450/69‑1 (MQ=255)
                                                                   gCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATa      >  1:4708379/1‑69 (MQ=255)
                                                                      gAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAgg  >  1:1565390/1‑70 (MQ=255)
                                                                      |                                                                     
CGTTACCCGCCGCATACATCAGCGAGAAACCGCCATCACGACGCGGATCGGTTGGCTCATACAGCTCGCCGAGCAGACAGCTTACGTTAGATTTAAACAGGCCATAGCCGCAGACGATAATCGCCAGGGACAGATAGAGG  >  Exported/454640‑454779

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: