Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 455,052 A→T 17.5% I11I (ATT→ATA ybgH ← predicted transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported455,0520AT17.5% 190.0 / 32.3 80I11I (ATT→ATAybgHpredicted transporter
Reads supporting (aligned to +/- strand):  ref base A (32/34);  new base T (6/8);  total (38/42)
Fisher's exact test for biased strand distribution p-value = 7.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01

GAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTCCACAATTGACGGCAACGTCACGTAATAGTT  >  Exported/454983‑455122
                                                                     |                                                                      
gagaATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTaaa                                                                       <  1:4334378/71‑1 (MQ=255)
gagaATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTaaa                                                                       >  1:328063/1‑71 (MQ=255)
gagaATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTaaa                                                                       <  1:3352644/71‑1 (MQ=255)
gagaATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTaaa                                                                       <  1:3796277/71‑1 (MQ=255)
gagaATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTaaa                                                                       <  1:4845549/71‑1 (MQ=255)
gagaATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTaaa                                                                       <  1:762769/71‑1 (MQ=255)
gagaATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTaaa                                                                       <  1:2087903/71‑1 (MQ=255)
gagaATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTaaa                                                                       <  1:2283969/71‑1 (MQ=255)
 agaATGAGTAAGGCACGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTatat                                                                      <  1:386759/71‑1 (MQ=255)
 agaATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTaaa                                                                       >  1:4696071/1‑70 (MQ=255)
    aTCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAg                                                                    <  1:1708427/70‑1 (MQ=255)
      cagcagGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAgcgc                                                                 >  1:4318729/1‑71 (MQ=255)
      cagcagGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAgcgc                                                                 >  1:2903474/1‑71 (MQ=255)
       agcagGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAgcgcg                                                                >  1:626790/1‑71 (MQ=255)
       agcagGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAgcgcg                                                                >  1:1768082/1‑71 (MQ=255)
        gcagGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTaaa                                                                       >  1:494033/1‑63 (MQ=255)
         taaggCACGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATcgcgcgcg                                                              >  1:4027102/4‑71 (MQ=255)
         cagGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAGcgcgcg                                                              >  1:925877/1‑71 (MQ=255)
          agGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCgg                                                             >  1:1929373/1‑71 (MQ=255)
          agGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCgg                                                             >  1:2968215/1‑71 (MQ=255)
          aaggCACGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCgg                                                             >  1:1049272/3‑71 (MQ=255)
          aaggCACGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCgg                                                             >  1:7948/3‑71 (MQ=255)
             gCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCtg                                                          <  1:3150876/71‑1 (MQ=255)
              cACGCATGCCGTAAAAGCTAAAATATTTCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCtgt                                                         <  1:1217570/71‑1 (MQ=255)
              cACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAg                                                                    >  1:957106/1‑60 (MQ=255)
              cACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCtgt                                                         <  1:4503315/71‑1 (MQ=255)
              cACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCtgt                                                         <  1:2786885/71‑1 (MQ=255)
              cACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCtgt                                                         <  1:4509645/71‑1 (MQ=255)
              cACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCtg                                                          <  1:1933962/70‑1 (MQ=255)
              cACGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCtgt                                                         <  1:3778408/71‑1 (MQ=255)
                cGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCTGTGa                                                       <  1:70855/71‑1 (MQ=255)
                cGCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGa                                                       <  1:294826/71‑1 (MQ=255)
                 gCATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGag                                                      >  1:2119683/1‑70 (MQ=255)
                  cATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGTTGTGATg                                                     >  1:3128724/1‑71 (MQ=255)
                  cATGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGagg                                                     <  1:121246/71‑3 (MQ=255)
                    tGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTatat                                                                      <  1:4975880/52‑1 (MQ=255)
                    tGCCGTAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGagg                                                     <  1:1998365/69‑3 (MQ=255)
                      ccGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCTGTGATGCgtg                                                 >  1:442478/1‑71 (MQ=255)
                         tAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTaaa                                                                       <  1:4982778/46‑1 (MQ=255)
                         tAAAAACTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCtgt                                                         <  1:5459242/60‑1 (MQ=255)
                           aaaaCTGAAGTACTCCCAGATTTGTATCGCCACGATATAGTATATCGCGCGCGGCTGTGag                                                      >  1:3834926/1‑60 (MQ=255)
                           aaaaCTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGAGGGTGttttc                                            <  1:1798406/70‑3 (MQ=255)
                           aaaaCTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGAGGGTGttttc                                            <  1:4056179/70‑3 (MQ=255)
                           aaaaCTGAAGTACTCCCAGATTTGGATCGCCACGATATAGTATATCGCGCGCGGCTGTGAGGGTGttttc                                            <  1:3652356/70‑3 (MQ=255)
                           aaaGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAg                                                                    >  1:1839905/1‑47 (MQ=255)
                           aaaGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCTGTGATGCGTGTTTAt                                            >  1:3473302/1‑71 (MQ=255)
                              gCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGc                                                            <  1:2532398/52‑1 (MQ=255)
                                tAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCtgt                                                         <  1:515501/53‑1 (MQ=255)
                                 aaaaTATTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCTGTGATGCGTGTTTa                                             >  1:4483838/1‑64 (MQ=255)
                                  aaaTATTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCTGTGATGCGTGTTTa                                             >  1:5443618/1‑63 (MQ=255)
                                  aaaTATTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCTGTGATGCGTGTTTATTCataat                                     <  1:4807160/71‑1 (MQ=255)
                                     tatTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCTGTGATGCGTGtt                                               <  1:1532654/58‑1 (MQ=255)
                                       ttCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTc                                <  1:4033631/71‑1 (MQ=255)
                                          ccAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTcc                               >  1:4394748/1‑69 (MQ=255)
                                          ccAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTCcac                             <  1:5599699/71‑1 (MQ=255)
                                          ccAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTCca                              >  1:3020669/1‑70 (MQ=255)
                                          ccAGATTTGCAGAGCAACGACATAGTAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTCca                              >  1:1175136/1‑70 (MQ=255)
                                           cAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTCcaca                            <  1:1510223/71‑1 (MQ=255)
                                            aGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCTGTGa                                                       >  1:2925206/1‑43 (MQ=255)
                                            aGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCTGTGa                                                       >  1:3796198/1‑43 (MQ=255)
                                            aGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTCCACaa                           >  1:4977867/1‑71 (MQ=255)
                                             gATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTCCACAAt                          >  1:5553338/1‑71 (MQ=255)
                                              aTTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTCCACAAtt                         <  1:5153035/71‑1 (MQ=255)
                                              aTTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTCCACAAtt                         >  1:1924153/1‑71 (MQ=255)
                                              aTTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTCCACAAtt                         <  1:3458865/71‑1 (MQ=255)
                                                ttGCAGCGCAACGACATAGTAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTCCACAATTGa                       >  1:5031569/1‑71 (MQ=255)
                                                ttGCAGCGCAACGACATAGTAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTCCACAATTGa                       >  1:2460373/1‑71 (MQ=255)
                                                ttGCAGAGCAACGACATAGTAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTCCACAATTGa                       >  1:737611/1‑71 (MQ=255)
                                                  gCAGCGCAACGACATAGTAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTCCACAATTGa                       <  1:5020296/69‑1 (MQ=255)
                                                   cAGCGCAACGACATAGTAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTCCACAATTGACgg                    >  1:3548632/1‑71 (MQ=255)
                                                    aGCGCAACGACATAGTAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTCCACAATTGACGGc                   <  1:1001850/71‑1 (MQ=255)
                                                     gcgcAACGACATAGTAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTCCACAATTGACGGc                   <  1:4602369/70‑1 (MQ=255)
                                                      cgcCACGATATAGTATATCGCGCGCGGCTGTGAGGGTGttttc                                            >  1:2435057/1‑41 (MQ=12)
                                                      cgcAACGACATAGTAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTCCACAATTGACGGCaa                 >  1:4018329/1‑71 (MQ=255)
                                                          aCGACATAGTAAATAGCGCGCGGCTGTGATGCGTGtt                                               <  1:940477/37‑1 (MQ=255)
                                                          aCGACATAGTAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTcc                               <  1:1744881/53‑1 (MQ=255)
                                                          aCGACATAGTAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTcc                               <  1:2095013/53‑1 (MQ=255)
                                                           cGACATAGTAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTCCACAAtt                         <  1:3388961/58‑1 (MQ=255)
                                                           cGACATAGTAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTCCACAAtt                         <  1:5311807/58‑1 (MQ=255)
                                                            gACATAGTAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTCcac                             >  1:1080846/1‑53 (MQ=255)
                                                            gACATAATAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTCCACAATTGACGGCAACGTCAc           <  1:1200868/71‑1 (MQ=255)
                                                             aCATAGTAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTCCACAATTGACGGCAACGTc             <  1:4935546/68‑1 (MQ=255)
                                                              cATAGTAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTCCACAATTGACGGCAACGTCACGt         <  1:4355098/71‑1 (MQ=255)
                                                               aTAGTAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTCCACAATTGACGGCAACGTCACGTa        <  1:5211152/71‑1 (MQ=255)
                                                               aTAGTAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTCCACAATTGACGGCAACGTCACGTa        <  1:1986489/71‑1 (MQ=255)
                                                                  gTAAATAGCGCGCGGCTGTGATGCGTGTTTATTCataat                                     <  1:923398/39‑1 (MQ=255)
                                                                  gTAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTCCACAATTGACGGCAACGTCACGTaa       >  1:5243582/1‑69 (MQ=255)
                                                                  gTAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTCCACAATTGACGGCAACGTCACGTaa       >  1:3092661/1‑69 (MQ=255)
                                                                   tAAATAGCGCGCGGCTGTGATGCGTGTTTATTCAtaataa                                   >  1:389757/1‑40 (MQ=255)
                                                                   tAAATAGCGCGCGGCTGTGATGCGTGTTTATTCAtaataa                                   >  1:512561/1‑40 (MQ=255)
                                                                   tAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTCCACAATTGACGGCAACGTCACGTaa       <  1:4625531/68‑1 (MQ=255)
                                                                   tAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTCCACAATTGACGGCAACGTCACGTaa       <  1:3794278/68‑1 (MQ=255)
                                                                   tAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTCCACAATTGACGGCAACGTCACGTaa       <  1:1133868/68‑1 (MQ=255)
                                                                    aaaTAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTCCACAATTGACGGCAACGTCACGTAATAGt   >  1:2178223/1‑71 (MQ=255)
                                                                     aaTAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTCCACAATTGACGGCAACGTCACGTAATAGtt  >  1:457941/1‑71 (MQ=255)
                                                                     |                                                                      
GAGAATCAGCAGGGCACGCATGCCGTAAAAGCTAAAATATTCCCAGATTTGCAGCGCAACGACATAGTAAATAGCGCGCGGCTGTGATGCGTGTTTATTCATAATAATTCCACAATTGACGGCAACGTCACGTAATAGTT  >  Exported/454983‑455122

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: