Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 509,780 T→A 5.4% intergenic (+5/‑178) ybhI → / → ybhJ predicted transporter/predicted hydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported509,7800TA 5.4% 312.5 / 5.9 108intergenic (+5/‑178)ybhI/ybhJpredicted transporter/predicted hydratase
Reads supporting (aligned to +/- strand):  ref base T (49/53);  new base A (3/3);  total (52/56)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.08e-01

Only 100 of 122 total aligned reads displayed.

CCTGGTGCATATCACCCTCGGCGTGTGGTGGTGGAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGC  >  Exported/509712‑509848
                                                                    |                                                                    
ccTGGTGCATATCACCCTCGGCGTGTGGTGGTGGAATATGCTGATCGGCTGGAACATGCTGTAAATATtct                                                                    >  1:1025600/1‑71 (MQ=255)
ccTGGTGCATATCACCCTCGGCGTGTGGTGGTGGAATATGCTGATCGGCTGGAACATGCTGTAAATATtct                                                                    >  1:41681/1‑71 (MQ=255)
    gTGCATATCACCCTCGGCGTGTGGTGGTGGAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGt                                                                 >  1:586725/1‑70 (MQ=255)
     tGCATATCACCCTCGGCGTGTGGTGGTGGAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCa                                                               <  1:5127270/71‑1 (MQ=255)
     tGCATATCACCCTCGGCGTGTGGTGGTGGAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCa                                                               <  1:4812986/71‑1 (MQ=255)
     tGCATATCACCCTCGGCGTGTGGTGGTGGAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCa                                                               <  1:3247413/71‑1 (MQ=255)
      gCATATCACCCTCGGCGTGTGGTGGTGGAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCa                                                               >  1:3832712/1‑70 (MQ=255)
          atCACCCTCGGCGTGTGGTGGTGGAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACt                                                           >  1:5338661/1‑70 (MQ=255)
           taaCCCTCGGCGTGTGGTGGTGGAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACtt                                                          >  1:127593/3‑70 (MQ=255)
           tCACCCTCGGCGTGTGGTGGTGGAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACtt                                                          >  1:3014239/1‑70 (MQ=255)
             aCCCTCGGCGTGTGGTGGTGGAATATGCTGCTCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTc                                                         >  1:2719721/1‑69 (MQ=255)
             aCCCTCGGCGTGTGGTGGTGGAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTc                                                         >  1:3326407/1‑69 (MQ=255)
             aCCCTCGGCGTGTGGTGGTGGAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCaa                                                       <  1:1004877/71‑1 (MQ=255)
             aCCCTCGGCGTGTGGTGGTGGAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCa                                                        <  1:114661/70‑1 (MQ=255)
             aCCCTCGGCGTGTGGTGGTGGAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCa                                                        <  1:2256359/70‑1 (MQ=255)
              cccTCGGCGTGTGGTGGTGGAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAg                                                      <  1:1214681/71‑1 (MQ=255)
              cccTCGGCGTGTGGTGGTGGAATATGCTGATCGGCTGGAACATGCTGTAAATATACTCGTCATACTTCAAg                                                      >  1:5120169/1‑71 (MQ=255)
              cccTCGGCGTGTGGTGGTGGAATATGCTGATCGGCTGGAACATGCTGTAAATATACTCGTCATACTTCAAg                                                      >  1:3044168/1‑71 (MQ=255)
              cccTCGGCGTGTGGTGGTGGAATATGCTGATCGGCTGGAACATGCTGTAAATATACTCGTCATACTTCAAg                                                      >  1:4522119/1‑71 (MQ=255)
               ccTCGGCGTGTGGTGGTGGAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACt                                                           <  1:480015/65‑1 (MQ=255)
                 tCGGCGTGTGGTGGTGGAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTg                                                   >  1:879217/1‑71 (MQ=255)
                 tCGGCGTGTGGTGGTGGAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTg                                                   >  1:3443797/1‑71 (MQ=255)
                  cGGCGTGTGGTGGTGGAATATGCTGATCGGCTGGAACATGCTGTAAATATtct                                                                    >  1:1467498/1‑53 (MQ=255)
                   ggCGTGTGGTGGTGGAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCa                                                               >  1:1101321/1‑57 (MQ=255)
                   ggCGTGTGGTGGTGGAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGc                                                  <  1:5573751/70‑1 (MQ=255)
                    gCGTGTGGTGGTGGAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCAt                                                <  1:609298/71‑1 (MQ=255)
                    gCGTGTGGTGGTGGAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCAt                                                <  1:4336770/71‑1 (MQ=255)
                     cGTGTGGTGGTGGAATATGCTGATCGGCTGGAACATGCTGTAAATAtt                                                                      <  1:1598005/48‑1 (MQ=255)
                         tggtggtggAATATGCTGATCGGCTGGAACATGCTGTAAATATtct                                                                    <  1:1846569/46‑1 (MQ=255)
                         tggtggtggAATATGCTGATCGGCTGGAACATGCTGTAAATATtct                                                                    <  1:2651605/46‑1 (MQ=255)
                         tggtggtggAATATGCTGATCGGCTGGAACATGCTGTAAATATtct                                                                    <  1:3684232/46‑1 (MQ=255)
                         tggtggtggAATATGCTGATCGGCTGGAACATGCTGTAAATATtct                                                                    <  1:5231676/46‑1 (MQ=255)
                         tggtggtggAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTg                                                   <  1:5255330/63‑1 (MQ=255)
                          ggtggtggAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTg                                                   <  1:4414153/62‑1 (MQ=255)
                           gtggtggAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCg                                                                  >  1:189775/1‑46 (MQ=255)
                            tggtggAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGctgcg                                        <  1:1314849/71‑1 (MQ=255)
                            tggtggAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGctgcg                                        <  1:3072311/71‑1 (MQ=255)
                              gtggAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGctgcgtc                                      >  1:2255932/1‑71 (MQ=255)
                               tggAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGctgcgtct                                     >  1:2917766/1‑71 (MQ=255)
                               tggAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGctgcgtct                                     <  1:5213687/71‑1 (MQ=255)
                               tggAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGctgcggct                                     <  1:3499539/71‑1 (MQ=255)
                               tggAATATGCTGATAGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTg                                                   <  1:1983083/57‑1 (MQ=255)
                                 gAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGt                                                     >  1:245073/1‑53 (MQ=255)
                                 gAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCAtg                                               >  1:3152272/1‑59 (MQ=255)
                                 gAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCAtg                                               >  1:4264117/1‑59 (MQ=255)
                                 gAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGctgcgtctgc                                   >  1:5389750/1‑71 (MQ=255)
                                 gAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGctgcgtctg                                    <  1:4563685/70‑1 (MQ=255)
                                   atatGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGctgcgtctgcgt                                 <  1:3158806/71‑1 (MQ=255)
                                    tatGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCAt                                                <  1:4286314/55‑1 (MQ=255)
                                      tGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCAt                                                >  1:5292089/1‑53 (MQ=255)
                                      tGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCAt                                                >  1:5354150/1‑53 (MQ=255)
                                      tGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCAt                                                >  1:3887572/1‑53 (MQ=255)
                                       gCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGGGTTCGc                             >  1:1816370/1‑71 (MQ=255)
                                       gCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGc                             >  1:4312324/1‑71 (MQ=255)
                                          gATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGctgcgtct                                     <  1:4504083/60‑1 (MQ=255)
                                           aTCGGCTGGAACATGCTGTAAATATTCTCGTCATa                                                             >  1:3338534/1‑35 (MQ=255)
                                           aTCGGCTGGAACATGCTGTAAATATTCTCGTCATa                                                             >  1:2091151/1‑35 (MQ=255)
                                           aTCGGCTGGAACATGCTGTAAATATTCTCGTCATa                                                             >  1:79831/1‑35 (MQ=255)
                                           aTCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCa                                                        <  1:2059511/40‑1 (MQ=255)
                                           aTCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCa                                                        <  1:2045742/40‑1 (MQ=255)
                                           aTCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGtgct                                           <  1:1948701/53‑1 (MQ=255)
                                           aTCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCAc                         >  1:4114385/1‑71 (MQ=255)
                                            tCGGCTGGTACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGtcctgc                                         <  1:865892/54‑1 (MQ=25)
                                            tCGGCTGGAACATGCTGTAAATATTCTCGTAATACTTCAAGTTGCATGtgctgc                                         <  1:1611532/54‑1 (MQ=255)
                                             cGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGtgc                                            >  1:5328267/1‑50 (MQ=255)
                                             cGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTc                               >  1:1504264/1‑63 (MQ=255)
                                             cGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCAccc                       >  1:1641784/1‑71 (MQ=255)
                                             cGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCAccc                       >  1:2338810/1‑71 (MQ=255)
                                             cGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCAccc                       >  1:5059687/1‑71 (MQ=255)
                                             cGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCAccc                       >  1:3245123/1‑71 (MQ=255)
                                              ggCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCAccc                       <  1:3040718/70‑1 (MQ=255)
                                                   gAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGt                   <  1:5541444/69‑1 (MQ=255)
                                                   gAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGt                   <  1:390914/69‑1 (MQ=255)
                                                   gAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGt                   <  1:5508383/69‑1 (MQ=255)
                                                   gAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGt                   <  1:3622603/69‑1 (MQ=255)
                                                   gAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCa                 <  1:5594135/71‑1 (MQ=255)
                                                     aCATGCTGTAAATATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCact               <  1:5525119/71‑1 (MQ=255)
                                                     aCATGCTGTAAATATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCact               <  1:3786743/71‑1 (MQ=255)
                                                       aTGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCactta             <  1:4185844/71‑1 (MQ=255)
                                                       aTGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCactta             <  1:3844019/71‑1 (MQ=255)
                                                       aTGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCactt              >  1:3590426/1‑70 (MQ=255)
                                                       aTGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCAACCCAGTCactta             <  1:5693374/71‑1 (MQ=255)
                                                        tGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCAcccc                      >  1:3472906/1‑61 (MQ=255)
                                                          cTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCacttact           <  1:5056631/70‑1 (MQ=255)
                                                            gTAAATATTCTCGTCATACTTCAAGTTGCATGtgc                                            >  1:2049805/1‑35 (MQ=255)
                                                             tAAATATTCTCGTCATACTTCAAGTTGCATGTGctgcg                                        <  1:4336350/38‑1 (MQ=255)
                                                             tAAATATTCTCGTCATACTTCAAGTTGCATGTGctgcg                                        <  1:4785928/38‑1 (MQ=255)
                                                             tAAATATTCTCGTCATACTTCAAGTTGCATGTGctgcg                                        <  1:463243/38‑1 (MQ=255)
                                                             tAAATATTCTCGTCATACTTCAAGTTGCATGTGctgcg                                        <  1:4439663/38‑1 (MQ=255)
                                                             tAAATATTCTCGTCATACTTCAAGTTGCATGTGctgcg                                        <  1:5627321/38‑1 (MQ=255)
                                                             tAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCAccc                       <  1:1002421/55‑1 (MQ=255)
                                                             tAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCActtactta         <  1:1834590/69‑1 (MQ=255)
                                                               aaTATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCg                              >  1:5362582/1‑46 (MQ=255)
                                                               aaTATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGc                             >  1:257486/1‑47 (MQ=255)
                                                               aaTATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGc                             >  1:861187/1‑47 (MQ=255)
                                                               aaTATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCAccc                       >  1:2259062/1‑53 (MQ=40)
                                                               aaTATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGt      <  1:4915030/70‑1 (MQ=39)
                                                               aaTATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGt      <  1:3301971/70‑1 (MQ=39)
                                                                  atTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGc  >  1:3927941/1‑71 (MQ=34)
                                                                    tctcGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGt                   <  1:4111234/52‑1 (MQ=31)
                                                                    |                                                                    
CCTGGTGCATATCACCCTCGGCGTGTGGTGGTGGAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGC  >  Exported/509712‑509848

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: