Predicted mutation | |||||||
---|---|---|---|---|---|---|---|
evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | Exported | 509,780 | T→A | 5.4% | intergenic (+5/‑178) | ybhI → / → ybhJ | predicted transporter/predicted hydratase |
Read alignment evidence... | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | Exported | 509,780 | 0 | T | A | 5.4% | 312.5 / 5.9 | 108 | intergenic (+5/‑178) | ybhI/ybhJ | predicted transporter/predicted hydratase |
Reads supporting (aligned to +/- strand): ref base T (49/53); new base A (3/3); total (52/56) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.08e-01 |
Only 100 of 122 total aligned reads displayed.
CCTGGTGCATATCACCCTCGGCGTGTGGTGGTGGAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGC > Exported/509712‑509848 | ccTGGTGCATATCACCCTCGGCGTGTGGTGGTGGAATATGCTGATCGGCTGGAACATGCTGTAAATATtct > 1:1025600/1‑71 (MQ=255) ccTGGTGCATATCACCCTCGGCGTGTGGTGGTGGAATATGCTGATCGGCTGGAACATGCTGTAAATATtct > 1:41681/1‑71 (MQ=255) gTGCATATCACCCTCGGCGTGTGGTGGTGGAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGt > 1:586725/1‑70 (MQ=255) tGCATATCACCCTCGGCGTGTGGTGGTGGAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCa < 1:5127270/71‑1 (MQ=255) tGCATATCACCCTCGGCGTGTGGTGGTGGAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCa < 1:4812986/71‑1 (MQ=255) tGCATATCACCCTCGGCGTGTGGTGGTGGAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCa < 1:3247413/71‑1 (MQ=255) gCATATCACCCTCGGCGTGTGGTGGTGGAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCa > 1:3832712/1‑70 (MQ=255) atCACCCTCGGCGTGTGGTGGTGGAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACt > 1:5338661/1‑70 (MQ=255) taaCCCTCGGCGTGTGGTGGTGGAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACtt > 1:127593/3‑70 (MQ=255) tCACCCTCGGCGTGTGGTGGTGGAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACtt > 1:3014239/1‑70 (MQ=255) aCCCTCGGCGTGTGGTGGTGGAATATGCTGCTCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTc > 1:2719721/1‑69 (MQ=255) aCCCTCGGCGTGTGGTGGTGGAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTc > 1:3326407/1‑69 (MQ=255) aCCCTCGGCGTGTGGTGGTGGAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCaa < 1:1004877/71‑1 (MQ=255) aCCCTCGGCGTGTGGTGGTGGAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCa < 1:114661/70‑1 (MQ=255) aCCCTCGGCGTGTGGTGGTGGAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCa < 1:2256359/70‑1 (MQ=255) cccTCGGCGTGTGGTGGTGGAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAg < 1:1214681/71‑1 (MQ=255) cccTCGGCGTGTGGTGGTGGAATATGCTGATCGGCTGGAACATGCTGTAAATATACTCGTCATACTTCAAg > 1:5120169/1‑71 (MQ=255) cccTCGGCGTGTGGTGGTGGAATATGCTGATCGGCTGGAACATGCTGTAAATATACTCGTCATACTTCAAg > 1:3044168/1‑71 (MQ=255) cccTCGGCGTGTGGTGGTGGAATATGCTGATCGGCTGGAACATGCTGTAAATATACTCGTCATACTTCAAg > 1:4522119/1‑71 (MQ=255) ccTCGGCGTGTGGTGGTGGAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACt < 1:480015/65‑1 (MQ=255) tCGGCGTGTGGTGGTGGAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTg > 1:879217/1‑71 (MQ=255) tCGGCGTGTGGTGGTGGAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTg > 1:3443797/1‑71 (MQ=255) cGGCGTGTGGTGGTGGAATATGCTGATCGGCTGGAACATGCTGTAAATATtct > 1:1467498/1‑53 (MQ=255) ggCGTGTGGTGGTGGAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCa > 1:1101321/1‑57 (MQ=255) ggCGTGTGGTGGTGGAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGc < 1:5573751/70‑1 (MQ=255) gCGTGTGGTGGTGGAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCAt < 1:609298/71‑1 (MQ=255) gCGTGTGGTGGTGGAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCAt < 1:4336770/71‑1 (MQ=255) cGTGTGGTGGTGGAATATGCTGATCGGCTGGAACATGCTGTAAATAtt < 1:1598005/48‑1 (MQ=255) tggtggtggAATATGCTGATCGGCTGGAACATGCTGTAAATATtct < 1:1846569/46‑1 (MQ=255) tggtggtggAATATGCTGATCGGCTGGAACATGCTGTAAATATtct < 1:2651605/46‑1 (MQ=255) tggtggtggAATATGCTGATCGGCTGGAACATGCTGTAAATATtct < 1:3684232/46‑1 (MQ=255) tggtggtggAATATGCTGATCGGCTGGAACATGCTGTAAATATtct < 1:5231676/46‑1 (MQ=255) tggtggtggAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTg < 1:5255330/63‑1 (MQ=255) ggtggtggAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTg < 1:4414153/62‑1 (MQ=255) gtggtggAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCg > 1:189775/1‑46 (MQ=255) tggtggAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGctgcg < 1:1314849/71‑1 (MQ=255) tggtggAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGctgcg < 1:3072311/71‑1 (MQ=255) gtggAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGctgcgtc > 1:2255932/1‑71 (MQ=255) tggAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGctgcgtct > 1:2917766/1‑71 (MQ=255) tggAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGctgcgtct < 1:5213687/71‑1 (MQ=255) tggAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGctgcggct < 1:3499539/71‑1 (MQ=255) tggAATATGCTGATAGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTg < 1:1983083/57‑1 (MQ=255) gAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGt > 1:245073/1‑53 (MQ=255) gAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCAtg > 1:3152272/1‑59 (MQ=255) gAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCAtg > 1:4264117/1‑59 (MQ=255) gAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGctgcgtctgc > 1:5389750/1‑71 (MQ=255) gAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGctgcgtctg < 1:4563685/70‑1 (MQ=255) atatGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGctgcgtctgcgt < 1:3158806/71‑1 (MQ=255) tatGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCAt < 1:4286314/55‑1 (MQ=255) tGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCAt > 1:5292089/1‑53 (MQ=255) tGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCAt > 1:5354150/1‑53 (MQ=255) tGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCAt > 1:3887572/1‑53 (MQ=255) gCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGGGTTCGc > 1:1816370/1‑71 (MQ=255) gCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGc > 1:4312324/1‑71 (MQ=255) gATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGctgcgtct < 1:4504083/60‑1 (MQ=255) aTCGGCTGGAACATGCTGTAAATATTCTCGTCATa > 1:3338534/1‑35 (MQ=255) aTCGGCTGGAACATGCTGTAAATATTCTCGTCATa > 1:2091151/1‑35 (MQ=255) aTCGGCTGGAACATGCTGTAAATATTCTCGTCATa > 1:79831/1‑35 (MQ=255) aTCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCa < 1:2059511/40‑1 (MQ=255) aTCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCa < 1:2045742/40‑1 (MQ=255) aTCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGtgct < 1:1948701/53‑1 (MQ=255) aTCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCAc > 1:4114385/1‑71 (MQ=255) tCGGCTGGTACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGtcctgc < 1:865892/54‑1 (MQ=25) tCGGCTGGAACATGCTGTAAATATTCTCGTAATACTTCAAGTTGCATGtgctgc < 1:1611532/54‑1 (MQ=255) cGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGtgc > 1:5328267/1‑50 (MQ=255) cGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTc > 1:1504264/1‑63 (MQ=255) cGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCAccc > 1:1641784/1‑71 (MQ=255) cGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCAccc > 1:2338810/1‑71 (MQ=255) cGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCAccc > 1:5059687/1‑71 (MQ=255) cGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCAccc > 1:3245123/1‑71 (MQ=255) ggCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCAccc < 1:3040718/70‑1 (MQ=255) gAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGt < 1:5541444/69‑1 (MQ=255) gAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGt < 1:390914/69‑1 (MQ=255) gAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGt < 1:5508383/69‑1 (MQ=255) gAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGt < 1:3622603/69‑1 (MQ=255) gAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCa < 1:5594135/71‑1 (MQ=255) aCATGCTGTAAATATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCact < 1:5525119/71‑1 (MQ=255) aCATGCTGTAAATATACTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCact < 1:3786743/71‑1 (MQ=255) aTGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCactta < 1:4185844/71‑1 (MQ=255) aTGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCactta < 1:3844019/71‑1 (MQ=255) aTGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCactt > 1:3590426/1‑70 (MQ=255) aTGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCAACCCAGTCactta < 1:5693374/71‑1 (MQ=255) tGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCAcccc > 1:3472906/1‑61 (MQ=255) cTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCacttact < 1:5056631/70‑1 (MQ=255) gTAAATATTCTCGTCATACTTCAAGTTGCATGtgc > 1:2049805/1‑35 (MQ=255) tAAATATTCTCGTCATACTTCAAGTTGCATGTGctgcg < 1:4336350/38‑1 (MQ=255) tAAATATTCTCGTCATACTTCAAGTTGCATGTGctgcg < 1:4785928/38‑1 (MQ=255) tAAATATTCTCGTCATACTTCAAGTTGCATGTGctgcg < 1:463243/38‑1 (MQ=255) tAAATATTCTCGTCATACTTCAAGTTGCATGTGctgcg < 1:4439663/38‑1 (MQ=255) tAAATATTCTCGTCATACTTCAAGTTGCATGTGctgcg < 1:5627321/38‑1 (MQ=255) tAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCAccc < 1:1002421/55‑1 (MQ=255) tAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCActtactta < 1:1834590/69‑1 (MQ=255) aaTATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCg > 1:5362582/1‑46 (MQ=255) aaTATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGc > 1:257486/1‑47 (MQ=255) aaTATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGc > 1:861187/1‑47 (MQ=255) aaTATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCAccc > 1:2259062/1‑53 (MQ=40) aaTATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGt < 1:4915030/70‑1 (MQ=39) aaTATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGt < 1:3301971/70‑1 (MQ=39) atTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGc > 1:3927941/1‑71 (MQ=34) tctcGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGt < 1:4111234/52‑1 (MQ=31) | CCTGGTGCATATCACCCTCGGCGTGTGGTGGTGGAATATGCTGATCGGCTGGAACATGCTGTAAATATTCTCGTCATACTTCAAGTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGC > Exported/509712‑509848 |
Alignment Legend |
---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |