Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 509,855 G→C 29.6% intergenic (+80/‑103) ybhI → / → ybhJ predicted transporter/predicted hydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported509,8550GC29.6% 181.3 / 90.1 124intergenic (+80/‑103)ybhI/ybhJpredicted transporter/predicted hydratase
Reads supporting (aligned to +/- strand):  ref base G (41/46);  new base C (19/17);  total (61/63)
Fisher's exact test for biased strand distribution p-value = 6.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.64e-01

Only 100 of 130 total aligned reads displayed.

GTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATATCCATTTATTATCTTTCTG  >  Exported/509796‑509922
                                                           |                                                                   
gTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGc                                                          <  1:3917907/71‑1 (MQ=32)
  tGCATGTACTGCGACTGCGTTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGCGATTCACTCGCtt                                                        >  1:2246424/1‑71 (MQ=11)
   gCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCtt                                                        <  1:4398392/70‑1 (MQ=35)
    cATGTACTGCGACTGCGTTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGCGATTCACTCGCTTg                                                       <  1:4247990/70‑1 (MQ=11)
     aTGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTc                                                     >  1:299285/1‑71 (MQ=34)
      tgtaCTGCGACTGCGTTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGCGATTCACTCGCTTGTc                                                     >  1:4104332/1‑70 (MQ=11)
      tgtaCTGCGACTGCGTTCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGCGATTCACTCGCTTGTCg                                                    >  1:2072234/1‑71 (MQ=11)
        tgctgcGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTCg                                                    >  1:1710652/1‑69 (MQ=35)
          ctgcgtctgcgtTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCt                                                 <  1:5105963/70‑1 (MQ=35)
          ctgcgtctgcgtTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCt                                                 <  1:2113211/70‑1 (MQ=35)
           tgcgtctgcgtTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGc                                                          >  1:2382228/1‑60 (MQ=33)
             cgtctgcgtTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCt                                             >  1:3089674/1‑71 (MQ=34)
             cgtctgcgtTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCt                                             >  1:4230004/1‑71 (MQ=34)
                 tgcgtTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCaa                                         >  1:1712573/1‑71 (MQ=34)
                 tgcgtTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCaa                                         >  1:1269331/1‑71 (MQ=34)
                  gcgtTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCt                                             >  1:2532864/1‑66 (MQ=35)
                  gcgtTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCa                                          >  1:3134181/1‑69 (MQ=35)
                   cgttcgttCACCCCAGTCACTTACTTATGTAAGCTCCTGGCGATTCACTCGCTTGTCGCCTTCCTGCAACt                                       >  1:2207467/1‑71 (MQ=255)
                    gtTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACt                                       >  1:1125081/1‑70 (MQ=35)
                    gtTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTc                                      <  1:986044/71‑1 (MQ=34)
                     ttCGTTCACCCCAGTCACTTACTTATGTAAGCTCCTGGCGATTCACTCGCTTGTCGCCTTCCTGCAACTCg                                     <  1:4017750/71‑1 (MQ=255)
                     ttCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCg                                     <  1:5316206/71‑1 (MQ=34)
                       cgttCACCCCAGTCACTTACTTATGTAAGCTCCTGGCGATTCACTCGCTTGTCGCCTTCCTGCAACTCGaa                                   >  1:354176/4‑71 (MQ=255)
                       cGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCGa                                    <  1:1349882/70‑1 (MQ=35)
                         cTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCGAatt                                 <  1:3511862/71‑1 (MQ=34)
                           cACCCCAGTCACTTACTTATGTAAGCTCCTGGCGATTCACTCg                                                           >  1:4693114/1‑43 (MQ=12)
                           cACCCCAGTCACTTACTTATGTAAGCTCCTGGCGATTCACTCGCTTGTCGCCTTCCTGCAACTCGAatta                                <  1:1246488/70‑1 (MQ=25)
                           cACCCCAGTCACTTACTTATGTAAGCTCCTGGCGATTCACTCGCTTGTCGCCTTCCTGCAACTCGAatta                                <  1:1828544/70‑1 (MQ=25)
                           cACCCCAGTCACTTACTTATGTAAGCTCCTGGCGATTCACTCGCTTGTCGCCTTCCTGCAACTCGAatta                                <  1:2914416/70‑1 (MQ=25)
                            aCCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCGAattatt                              >  1:436627/1‑71 (MQ=34)
                            aCCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCGAattatt                              >  1:1640462/1‑71 (MQ=34)
                            aCCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCGAattatt                              <  1:1210634/71‑1 (MQ=34)
                            aCCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCGAattat                               <  1:4421238/70‑1 (MQ=35)
                            aCCCCAGTCACTTACTTATGTAAGCTCCTGGCGATTCACTCGCTTGTCGCCTTCCTGCAACTCGAattatt                              <  1:5577780/71‑1 (MQ=21)
                            aCCCCAGTCACTTACTTATGTAAGCTCCTGGCGATTCACTCGCTTGTCGCCTTCCTGCAACTCGAattatt                              <  1:4779509/71‑1 (MQ=21)
                            aCCCCAGTAACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCGAattatt                              <  1:2074592/71‑1 (MQ=21)
                              cccAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCGAATTATTTa                            <  1:4451343/71‑1 (MQ=34)
                                cAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCt                                                 <  1:1298574/48‑1 (MQ=38)
                                cAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCGAATTATTTa                            >  1:2183000/1‑69 (MQ=35)
                                 aGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCGAat                                  >  1:4479666/1‑62 (MQ=35)
                                  gTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGt                        <  1:638039/71‑1 (MQ=34)
                                  gTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGt                        <  1:5032965/71‑1 (MQ=34)
                                   ttacttacttATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCg                                     <  1:1422843/55‑1 (MQ=25)
                                   tCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCg                                     <  1:161930/57‑1 (MQ=35)
                                    cACTTACTTATGTAAGCTCCTGGGGATTCACTCGCtt                                                        >  1:2002846/1‑37 (MQ=40)
                                    cACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCGAATTATTTa                            >  1:2058878/1‑65 (MQ=35)
                                    cACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGtat                      >  1:870343/1‑71 (MQ=34)
                                    cACTTACTTATGTAAGCTCCTGGCGATTCACTCGCTTGTCg                                                    >  1:4597285/1‑41 (MQ=255)
                                     acttacttATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCGAATTAttt                             <  1:2585870/63‑1 (MQ=35)
                                     acttacttATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGtata                     >  1:416697/1‑71 (MQ=35)
                                     acttacttATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGtata                     >  1:5515768/1‑71 (MQ=35)
                                     acttacttATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGtat                      <  1:5058157/70‑1 (MQ=35)
                                     acttacttATGTAAGCTCCTGGCGATTCACTCGCTTGTCGCCTTc                                               >  1:2311532/1‑45 (MQ=255)
                                     acttacttATGTAAGCTCCTGGCGATTCACTCGCTTGTCGCCTTCCTGCAAc                                        >  1:1850745/1‑52 (MQ=255)
                                     acgtacttcTGTACGCTCCCGGGGATTCACTCACTTGCCGCCTTGATGCAACCTGAATGATTTTGTGtata                     >  1:3672658/4‑71 (MQ=2)
                                      cgtacttcTGTACGCTCCCGGGGATTCACTCACTTGCCGCCTTGATGCAACCTGAATGATTTTGTGTatat                    >  1:5650521/3‑71 (MQ=2)
                                       gtacttcTGTACGCTCCAGGGGATTCACTCACTTGCCGCCTTGATGCAACCTGAATGATTTTGTGTatat                    >  1:3334416/2‑70 (MQ=2)
                                        tacttaTGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTc                                               >  1:82524/1‑42 (MQ=38)
                                        tacttaTGTAAGCTCCTGGCGATTCACTCGCTTGTCGCCTTCCTGCAACTCGAattatt                              <  1:3396562/59‑1 (MQ=255)
                                         acttcTGTACGCTCCCGGGGATTCACTCACTTGCCGCCTTGATGCAACCTGAATGATTTTGTGTatattac                 >  1:128915/1‑68 (MQ=2)
                                         acttaTGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTc                                               >  1:4853886/1‑41 (MQ=38)
                                         acttaTGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCGAattat                               >  1:4677619/1‑57 (MQ=35)
                                          cttaTGTAAGCTCCTGGGGATTCACTCGCTTGTCGc                                                   <  1:3119611/36‑1 (MQ=40)
                                          cttaTGTAAGCTCCTGGGGATTCACTCGCTTGTCGc                                                   <  1:1399226/36‑1 (MQ=40)
                                             aTGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCGAattatt                              <  1:358457/54‑1 (MQ=35)
                                             aTGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCGAattatt                              <  1:2641988/54‑1 (MQ=35)
                                              tGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCg                                     <  1:3287929/46‑1 (MQ=38)
                                              tGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGtatga                    <  1:2033647/63‑3 (MQ=14)
                                               gTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCGa                                    <  1:2312650/46‑1 (MQ=38)
                                               gTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCGa                                    <  1:2878486/46‑1 (MQ=38)
                                               gTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCGa                                    <  1:1611926/46‑1 (MQ=38)
                                               gTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCGa                                    <  1:1582643/46‑1 (MQ=38)
                                                tAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCaa                                         >  1:4795826/1‑40 (MQ=40)
                                                tAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATATCCATttatt           <  1:981129/70‑1 (MQ=255)
                                                tAAGCTCCTGGCGATTCACTCGCTTGTCGCCTTCCTGCa                                          <  1:3768352/39‑1 (MQ=255)
                                                tAAGCTCCTGGCGATTCACTCGCTTGTCGCCTTCCTGCa                                          <  1:2480809/39‑1 (MQ=255)
                                                tAAGCTCCTGGCGATTCACTCGCTTGTCGCCTTCCTGCAACt                                       >  1:5490590/1‑42 (MQ=255)
                                                tAAGCTCCTGGCGATTCACTCGCTTGTCGCCTTCCTGCAACTCg                                     <  1:353728/44‑1 (MQ=255)
                                                tAAGCTCCTGGCGATTCACTCGCTTGTCGCCTTCCTGCAACTCGAATTATTTa                            >  1:2407092/1‑53 (MQ=255)
                                                tAAGCTCCTGGCGATTCACTCGCTTGTCGCCTTCCTGCAACTCGAATTATTTa                            >  1:2430178/1‑53 (MQ=255)
                                                tAAGCGCCTGGCGATTCACTCGCTTGTCGCCTTCCTGCa                                          <  1:2120812/39‑1 (MQ=14)
                                                  aGCTCCTGGCGATTCACTCGCTTGTCGCCTTCCTGCAACTCGaa                                   >  1:2670201/1‑44 (MQ=255)
                                                  aGCTCCAGGCGATTCACTCGCTTGTCGCCTTCCTGCAAc                                        >  1:1778269/1‑39 (MQ=14)
                                                   gCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCg                                     <  1:3209447/41‑1 (MQ=38)
                                                   gCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCg                                     <  1:3396318/41‑1 (MQ=38)
                                                   gCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCg                                     <  1:3969627/41‑1 (MQ=38)
                                                   gCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATATCCATTTATTATCt       <  1:2140181/71‑1 (MQ=255)
                                                   gCTCCTGGCGATTCACTCGCTTGTCGCCTTCCTGCAACTCGAattatt                              <  1:2852966/48‑1 (MQ=255)
                                                      ccTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCGAATTATTTa                            >  1:5409363/1‑47 (MQ=38)
                                                       cTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCGAatta                                <  1:5241402/42‑1 (MQ=38)
                                                       cTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCGAATTATTTagag                         <  1:5452214/49‑1 (MQ=38)
                                                       cTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCGAATTATTTag                           <  1:3288349/47‑1 (MQ=38)
                                                       cTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATATCCAttt              <  1:3773579/60‑1 (MQ=255)
                                                        tGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCGAATTAttt                             <  1:110257/44‑1 (MQ=38)
                                                         ggggATTCACTCGCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATATCCATTTATTATCTTTCTg  >  1:3696160/1‑70 (MQ=255)
                                                          gggATTCACTCGCTTGTCGCCTTCCTGCAACTCGAattatt                              <  1:5470878/41‑1 (MQ=38)
                                                           ggATTCACTCGCTTGTCGCCTTCCTGCAACTCGAattatt                              <  1:5017113/40‑1 (MQ=40)
                                                           ggATTCACTCGCTTGTCGCCTTCCTGCAACTCGAATTATTTa                            <  1:5200021/42‑1 (MQ=38)
                                                           ggATTCACTCGCTTGTCGCCTTCCTGCAACTCGAATTATTTa                            <  1:796653/42‑1 (MQ=38)
                                                           ggATTCACTCGCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATATCCATttat            >  1:3390539/1‑58 (MQ=255)
                                                           |                                                                   
GTTGCATGTGCTGCGTCTGCGTTCGCTCACCCCAGTCACTTACTTATGTAAGCTCCTGGGGATTCACTCGCTTGTCGCCTTCCTGCAACTCGAATTATTTAGAGTATATCCATTTATTATCTTTCTG  >  Exported/509796‑509922

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: