| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 538,787 | A→G | 8.8% | intergenic (+96/+538) | rhlE → / ← dps | RNA helicase/Fe‑binding and storage protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 538,787 | 0 | A | G | 8.8% | 220.6 / 9.2 | 79 | intergenic (+96/+538) | rhlE/dps | RNA helicase/Fe‑binding and storage protein |
| Reads supporting (aligned to +/- strand): ref base A (42/30); new base G (4/3); total (46/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.17e-01 | |||||||||||
AGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCAC > Exported/538720‑538857 | aGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAatatat > 1:5403778/1‑71 (MQ=255)aGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAatatat > 1:4804154/1‑71 (MQ=255)aGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAatatat > 1:3529830/1‑71 (MQ=255) gCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAatatat > 1:2020415/1‑69 (MQ=255) atgaACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGaa < 1:886289/69‑1 (MQ=255) atgaACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGaa < 1:266493/69‑1 (MQ=255) gaACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTg > 1:344676/1‑71 (MQ=255) gaACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTg > 1:3513849/1‑71 (MQ=255) aaCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTg > 1:1009013/1‑70 (MQ=255) aaCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTg > 1:2845064/1‑70 (MQ=255) aaCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTg > 1:1449450/1‑70 (MQ=255) aCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCa > 1:4482173/1‑71 (MQ=255) aCAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCa > 1:5458801/1‑71 (MQ=255) cAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGa > 1:3230343/1‑63 (MQ=255) cAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGa > 1:1146460/1‑63 (MQ=255) cAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGa > 1:2185690/1‑63 (MQ=255) cAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCAt > 1:1691513/1‑71 (MQ=255) aCGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAata < 1:1672785/54‑1 (MQ=255) aCGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAata < 1:222489/54‑1 (MQ=255) gCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCttt < 1:5315014/71‑1 (MQ=255) cAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCtttt < 1:264633/71‑1 (MQ=255) aaaaCTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTCTg > 1:1009757/1‑71 (MQ=255) aaaaCTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTATTg > 1:549139/1‑71 (MQ=255) aaaCTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCttt < 1:4702484/68‑1 (MQ=255) aaaCTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTg > 1:706270/1‑70 (MQ=255) aaCTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTg > 1:1098510/1‑69 (MQ=255) aCTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCtttt > 1:5410900/1‑67 (MQ=255) aCTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAg > 1:2288712/1‑71 (MQ=255) aCTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAg < 1:622238/71‑1 (MQ=255) aCTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAg > 1:1438223/1‑71 (MQ=255) aCTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAg > 1:3133039/1‑71 (MQ=255) aCTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAg < 1:3098963/71‑1 (MQ=255) ccTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCg > 1:2099705/1‑71 (MQ=255) ccTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCg < 1:4185033/71‑1 (MQ=255) ccTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCg > 1:4025865/1‑71 (MQ=255) ccTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCg < 1:2286866/71‑1 (MQ=255) gATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCgg > 1:2246883/1‑69 (MQ=255) gATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGa > 1:885521/1‑70 (MQ=255) gATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGa > 1:1793241/1‑70 (MQ=255) aTGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAata > 1:2881302/1‑39 (MQ=255) aTGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAata > 1:2048202/1‑39 (MQ=255) aTGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGc > 1:2597959/1‑51 (MQ=255) aTGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGAt < 1:4681069/70‑1 (MQ=255) aTGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGAt < 1:1446537/70‑1 (MQ=255) tGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATaa < 1:263348/71‑1 (MQ=255) cgcTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGc < 1:3115061/62‑1 (MQ=255) cgcTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAgg > 1:3476652/1‑71 (MQ=255) cgcTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAg < 1:2630273/70‑1 (MQ=255) cTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTa > 1:1738902/1‑57 (MQ=255) cTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCg > 1:5196309/1‑62 (MQ=255) tACGCTTATCAGGCCTACGTGAACTCTGCAATATATTg < 1:2743006/38‑1 (MQ=255) cGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTg < 1:4744946/42‑1 (MQ=255) cGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTg < 1:2827915/42‑1 (MQ=255) cGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCtttt > 1:3790145/1‑51 (MQ=255) gCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGtt < 1:4961625/69‑1 (MQ=255) cTTATCAGGCCTACGTGAACTCTGCAATATATTGAAttt > 1:3566313/1‑39 (MQ=255) cTTATCAGGCCTACGTGAACTCTGCAATATATTGAAttt > 1:2299777/1‑39 (MQ=255) aTCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTAcgcc > 1:169200/1‑71 (MQ=255) gCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATc < 1:1674553/71‑1 (MQ=255) cTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCg > 1:3667419/1‑71 (MQ=255) cTACGTGAACTCTGCAATATATTGAATTTGAATGCTTTTGTAGGCAGGATaa > 1:1594758/1‑52 (MQ=14) tACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTa > 1:425733/1‑59 (MQ=255) cGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCgg < 1:1464454/69‑1 (MQ=255) cGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCgg < 1:942636/69‑1 (MQ=255) cGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCgg < 1:4783723/69‑1 (MQ=255) gTGAACTCTGCAATATATTGAATTTGCATGCTTTTg < 1:1207570/36‑1 (MQ=255) gTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAt < 1:1088517/71‑1 (MQ=255) tGAACTCTGCAATATATTGAATTTGCATGCTTTTGt > 1:899254/1‑36 (MQ=255) tGAACTCTGCAATATATTGAATTTGCATGCTTTTGt > 1:4655884/1‑36 (MQ=255) tGAACTCTGCAATATATTGAATTTGCATGCTTTTGt > 1:892597/1‑36 (MQ=255) tGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAtt > 1:873523/1‑71 (MQ=255) gAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGATTACGCCGCATCCGGCAttt > 1:46906/1‑71 (MQ=255) aaCTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAttt < 1:4354799/70‑1 (MQ=255) aaCTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAttt < 1:5217807/70‑1 (MQ=255) aaCTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAttt < 1:2994025/70‑1 (MQ=255) aCTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAttt < 1:3631997/69‑1 (MQ=255) ctctGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGa > 1:5240586/1‑40 (MQ=40) tctGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTcac > 1:2133937/1‑70 (MQ=255) tGCAATGTATTGAATTTGCACGCTTTTGTAGGCCGGAt < 1:5491886/38‑1 (MQ=14) tGCAATGTATTGAATTTGCACGCTTTTGTAGGCCGGAt < 1:3995064/38‑1 (MQ=14) tGCAATGTATTGAATTTGCACGCTTTTGTAGGCCGGATAAGGCGtt < 1:1410310/46‑1 (MQ=11) cAATGTATTGAATTTGCACGCTTTTGTAGGCCGGAt > 1:827221/1‑36 (MQ=14) cAATGTATTGAATTTGCACGCTTTTGTAGGCCGGATaa > 1:3643886/1‑38 (MQ=14) cAATGTATTGAATTTGCACGCTTTTGTAGGCCGGATAAGGCGTTTa > 1:5063152/1‑46 (MQ=11) cAATGTATTGAATTTGCACGCTTTTGTAGGCCGGATAAGGCGTTTa > 1:3314255/1‑46 (MQ=11) aaTATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAg < 1:955801/71‑1 (MQ=255) aaTATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAg < 1:4574589/71‑1 (MQ=255) atatatTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGtt < 1:4451630/42‑1 (MQ=38) atatTGAATTTGGATGCTTTTGTAGGCCGGATAAGGCGTTTa > 1:5085013/1‑42 (MQ=255) atatTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTa > 1:4561344/1‑42 (MQ=255) atatTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCAc > 1:5510004/1‑71 (MQ=255) | AGGCTGATGAACAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCAC > Exported/538720‑538857 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |