Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 538,801 T→C 10.8% intergenic (+110/+524) rhlE → / ← dps RNA helicase/Fe‑binding and storage protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported538,8010TC10.8% 199.8 / 12.3 74intergenic (+110/+524)rhlE/dpsRNA helicase/Fe‑binding and storage protein
Reads supporting (aligned to +/- strand):  ref base T (34/32);  new base C (5/3);  total (39/35)
Fisher's exact test for biased strand distribution p-value = 7.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

CAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGT  >  Exported/538731‑538861
                                                                      |                                                            
cAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCAt                                                              >  1:1691513/1‑71 (MQ=255)
     gCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCttt                                                         <  1:5315014/71‑1 (MQ=255)
      cAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCtttt                                                        <  1:264633/71‑1 (MQ=255)
       aaaaCTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTCTg                                                       >  1:1009757/1‑71 (MQ=255)
       aaaaCTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTATTg                                                       >  1:549139/1‑71 (MQ=255)
        aaaCTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCttt                                                         <  1:4702484/68‑1 (MQ=255)
        aaaCTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTg                                                       >  1:706270/1‑70 (MQ=255)
         aaCTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTg                                                       >  1:1098510/1‑69 (MQ=255)
          aCTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCtttt                                                        >  1:5410900/1‑67 (MQ=255)
          aCTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAg                                                    >  1:2288712/1‑71 (MQ=255)
          aCTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAg                                                    <  1:622238/71‑1 (MQ=255)
          aCTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAg                                                    >  1:1438223/1‑71 (MQ=255)
          aCTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAg                                                    >  1:3133039/1‑71 (MQ=255)
          aCTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAg                                                    <  1:3098963/71‑1 (MQ=255)
              ccTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCg                                                >  1:2099705/1‑71 (MQ=255)
              ccTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCg                                                <  1:2286866/71‑1 (MQ=255)
              ccTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCg                                                <  1:4185033/71‑1 (MQ=255)
              ccTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCg                                                >  1:4025865/1‑71 (MQ=255)
                 gATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCgg                                               >  1:2246883/1‑69 (MQ=255)
                 gATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGa                                              >  1:885521/1‑70 (MQ=255)
                 gATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGa                                              >  1:1793241/1‑70 (MQ=255)
                  aTGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGAt                                             <  1:4681069/70‑1 (MQ=255)
                  aTGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGAt                                             <  1:1446537/70‑1 (MQ=255)
                   tGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATaa                                           <  1:263348/71‑1 (MQ=255)
                     cgcTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGc                                                  <  1:3115061/62‑1 (MQ=255)
                     cgcTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAgg                                         >  1:3476652/1‑71 (MQ=255)
                     cgcTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAg                                          <  1:2630273/70‑1 (MQ=255)
                       cTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTa                                                     >  1:1738902/1‑57 (MQ=255)
                       cTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCg                                                >  1:5196309/1‑62 (MQ=255)
                          cGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCtttt                                                        >  1:3790145/1‑51 (MQ=255)
                           gCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGtt                                     <  1:4961625/69‑1 (MQ=255)
                               aTCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTAcgcc                               >  1:169200/1‑71 (MQ=255)
                                    gCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATc                          <  1:1674553/71‑1 (MQ=255)
                                      cTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCg                        >  1:3667419/1‑71 (MQ=255)
                                      cTACGTGAACTCTGCAATATATTGAATTTGAATGCTTTTGTAGGCAGGATaa                                           >  1:1594758/1‑52 (MQ=14)
                                       tACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTa                                   >  1:425733/1‑59 (MQ=255)
                                         cGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCgg                       <  1:4783723/69‑1 (MQ=255)
                                         cGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCgg                       <  1:1464454/69‑1 (MQ=255)
                                         cGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCgg                       <  1:942636/69‑1 (MQ=255)
                                          gTGAACTCTGCAATATATTGAATTTGCATGCTTTTg                                                       <  1:1207570/36‑1 (MQ=255)
                                          gTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAt                    <  1:1088517/71‑1 (MQ=255)
                                           tGAACTCTGCAATATATTGAATTTGCATGCTTTTGt                                                      >  1:4655884/1‑36 (MQ=255)
                                           tGAACTCTGCAATATATTGAATTTGCATGCTTTTGt                                                      >  1:892597/1‑36 (MQ=255)
                                           tGAACTCTGCAATATATTGAATTTGCATGCTTTTGt                                                      >  1:899254/1‑36 (MQ=255)
                                           tGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAtt                   >  1:873523/1‑71 (MQ=255)
                                            gAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGATTACGCCGCATCCGGCAttt                  >  1:46906/1‑71 (MQ=255)
                                             aaCTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAttt                  <  1:4354799/70‑1 (MQ=255)
                                             aaCTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAttt                  <  1:2994025/70‑1 (MQ=255)
                                             aaCTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAttt                  <  1:5217807/70‑1 (MQ=255)
                                              aCTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAttt                  <  1:3631997/69‑1 (MQ=255)
                                               ctctGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGa                                              >  1:5240586/1‑40 (MQ=40)
                                                tctGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTcac               >  1:2133937/1‑70 (MQ=255)
                                                  tGCAATGTATTGAATTTGCACGCTTTTGTAGGCCGGAt                                             <  1:3995064/38‑1 (MQ=14)
                                                  tGCAATGTATTGAATTTGCACGCTTTTGTAGGCCGGAt                                             <  1:5491886/38‑1 (MQ=14)
                                                  tGCAATGTATTGAATTTGCACGCTTTTGTAGGCCGGATAAGGCGtt                                     <  1:1410310/46‑1 (MQ=11)
                                                    cAATGTATTGAATTTGCACGCTTTTGTAGGCCGGAt                                             >  1:827221/1‑36 (MQ=14)
                                                    cAATGTATTGAATTTGCACGCTTTTGTAGGCCGGATaa                                           >  1:3643886/1‑38 (MQ=14)
                                                    cAATGTATTGAATTTGCACGCTTTTGTAGGCCGGATAAGGCGTTTa                                   >  1:5063152/1‑46 (MQ=11)
                                                    cAATGTATTGAATTTGCACGCTTTTGTAGGCCGGATAAGGCGTTTa                                   >  1:3314255/1‑46 (MQ=11)
                                                     aaTATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAg         <  1:955801/71‑1 (MQ=255)
                                                     aaTATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAg         <  1:4574589/71‑1 (MQ=255)
                                                      atatatTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGtt                                     <  1:4451630/42‑1 (MQ=38)
                                                        atatTGAATTTGGATGCTTTTGTAGGCCGGATAAGGCGTTTa                                   >  1:5085013/1‑42 (MQ=255)
                                                        atatTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTa                                   >  1:4561344/1‑42 (MQ=255)
                                                        atatTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCAc      >  1:5510004/1‑71 (MQ=255)
                                                         tatTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGtt                                     <  1:281175/39‑1 (MQ=40)
                                                         tatTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCAc      <  1:4569740/70‑1 (MQ=255)
                                                         tatTGAATTTGCACGCTTTTGTAGGCCGGATAAGGCGTTTa                                   >  1:3238293/1‑41 (MQ=255)
                                                           ttGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGtt                                     <  1:3471444/37‑1 (MQ=40)
                                                           ttGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGtt                                     <  1:1659299/37‑1 (MQ=40)
                                                           ttGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGtt                                     <  1:1652674/37‑1 (MQ=40)
                                                           ttGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTa                                   >  1:1866687/1‑39 (MQ=255)
                                                           ttGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACtt    <  1:121407/70‑1 (MQ=255)
                                                             gAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGt  >  1:2533837/1‑70 (MQ=255)
                                                                   gCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTcac               >  1:708396/1‑51 (MQ=255)
                                                                      |                                                            
CAAACGCAAAACTGCCTGATGCGCTACGCTTATCAGGCCTACGTGAACTCTGCAATATATTGAATTTGCATGCTTTTGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCATTTCACAACAAGCACTTGT  >  Exported/538731‑538861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: