Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	608,316	T→C	27.7%	V646V (GTA→GTG)	pflB ←	pyruvate formate lyase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	608,316	0	T	C	27.7%	92.2 / 41.3	65	V646V (GTA→GTG)	pflB	pyruvate formate lyase I
Reads supporting (aligned to +/- strand):  ref base T (28/19);  new base C (12/6);  total (40/25)
Fisher's exact test for biased strand distribution p-value = 7.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACG  >  Exported/608247‑608384                                                                      |                                                                     agagAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTa                                                                     >  1:4378990/1‑71 (MQ=255) agagAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTa                                                                     >  1:2969125/1‑71 (MQ=255) agagAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTa                                                                     >  1:5573347/1‑71 (MQ=255)    gaAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTg                                                                  <  1:920718/71‑1 (MQ=255)     aaGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGc                                                                 <  1:3026919/71‑1 (MQ=255)     aaGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGc                                                                 <  1:3033685/71‑1 (MQ=255)        gtgtAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCAcc                                                              >  1:397857/1‑71 (MQ=255)          gtAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCtt                                                            >  1:267150/1‑71 (MQ=255)             ggAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCt                                                         >  1:415944/1‑71 (MQ=255)              gagaTCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCtttgcg                                                        <  1:834402/71‑4 (MQ=255)              gagaTCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCtttgcg                                                        >  1:1693457/1‑68 (MQ=255)              gagaTCCCATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCtttgcg                                                        >  1:4114886/1‑68 (MQ=255)              gagaTACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTa                                                                     >  1:3596066/1‑58 (MQ=255)              gagaTACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTa                                                                     >  1:4826911/1‑58 (MQ=255)                   aCCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCAc                                                   >  1:5691076/1‑71 (MQ=255)                    ccATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCAc                                                   >  1:3321072/1‑70 (MQ=255)                    ccATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCAc                                                   >  1:3762863/1‑70 (MQ=255)                    ccATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTgg                                                       <  1:1633976/66‑1 (MQ=255)                    ccATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCAc                                                   >  1:526394/1‑70 (MQ=255)                     cATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGa                                                 >  1:196632/1‑71 (MQ=255)                     cATCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGa                                                 >  1:489970/1‑71 (MQ=255)                      aTCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGAc                                                <  1:1258203/71‑1 (MQ=255)                       tCTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGATCACGAc                                                >  1:432341/1‑70 (MQ=255)                        cTTTGGCGTAGGTGAACGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGAcca                                              >  1:5093733/1‑70 (MQ=255)                          ttAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGt                                             >  1:3836571/1‑70 (MQ=255)                          ttAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTg                                            >  1:1192396/1‑71 (MQ=255)                          ttAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCCTg                                            >  1:4201025/1‑71 (MQ=255)                           tAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGAcc                                               <  1:635675/67‑1 (MQ=255)                           tAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGt                                             <  1:2513253/69‑1 (MQ=255)                           tAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTg                                            >  1:3385579/1‑70 (MQ=255)                              cGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATc                                        <  1:3595044/71‑1 (MQ=255)                               gTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCg                                       >  1:5471197/1‑71 (MQ=255)                                tAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCg                                       <  1:2509113/70‑1 (MQ=255)                                  aGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGAc                                                >  1:3140718/1‑59 (MQ=255)                                  aGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGAc                                                >  1:4945308/1‑59 (MQ=255)                                  aGCAAACGGCAGTTTAGAAACGGAAGTCAGAGAGGCTACTCCACCTTTCTGGTAACGACCGTGCATCGGGt                                    >  1:4693906/1‑71 (MQ=255)                                      aaCGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCATGCATCGGGTTAGc                                >  1:2058665/1‑71 (MQ=255)                                       aCGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCATGCATCGGGTTAGcg                               <  1:945225/71‑2 (MQ=255)                                       aCGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCATGCATCGGGTTAGcg                               >  1:321985/1‑70 (MQ=255)                                       aCGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCATGCATCGGGTTAGcg                               >  1:3138578/1‑70 (MQ=255)                                       aCGGCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCATGCATCGGGTTAGcg                               >  1:2844125/1‑70 (MQ=255)                                       aCGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGt                                                      >  1:2937546/1‑48 (MQ=255)                                       aCGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGc                                >  1:1711072/1‑70 (MQ=255)                                         ggCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCATGc                                           <  1:3407023/57‑1 (MQ=255)                                         ggCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCATGc                                           <  1:2386775/57‑1 (MQ=255)                                          gCAGTTTCGCCACCGACGTCAATGAGGCCACGGCACCTTTGCGGTCACGACCATGCATCGGGTTAGCGccc                            <  1:1432672/71‑1 (MQ=255)                                            aGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCgg                          <  1:3073683/71‑1 (MQ=255)                                            aGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCgg                          <  1:1762056/71‑1 (MQ=255)                                                  gCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGaa                    <  1:2120626/71‑1 (MQ=255)                                                   cAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCAc                                                   >  1:1451238/1‑39 (MQ=255)                                                      cGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGgc                <  1:5113031/71‑1 (MQ=255)                                                       ggAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCAccc                            >  1:2368787/1‑58 (MQ=255)                                                       ggAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCAccc                            >  1:1922208/1‑58 (MQ=255)                                                        gAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGgcgc              <  1:3688099/71‑1 (MQ=255)                                                          aGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGgcgcgc            >  1:980134/1‑71 (MQ=255)                                                          aGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGgcgcgc            >  1:3105067/1‑71 (MQ=255)                                                            tCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGt                                    <  1:5463821/45‑1 (MQ=255)                                                                tgagGCCACGGCACCTTTGCGGTCACGACCATGCATCGGGtt                                   <  1:900613/41‑1 (MQ=255)                                                                agagGCTACTGCACCTTTCTGGTCACGACCGTGCAt                                         >  1:2995951/1‑36 (MQ=255)                                                                agagGCTACTGCACCTTTCTGGTCACGACCGTGCAt                                         >  1:3128837/1‑36 (MQ=255)                                                                agagGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCac      <  1:2490337/71‑1 (MQ=255)                                                                agagGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCac      <  1:1399283/71‑1 (MQ=255)                                                                   ggCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCacga    >  1:2161674/1‑70 (MQ=255)                                                                    gCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCacgacg  <  1:3342709/71‑1 (MQ=255)                                                                     cTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAg                                 <  1:1676881/39‑1 (MQ=255)                                                                      |                                                                     AGAGAAGGTGTAGGAGATACCATCTTTAGCGTAAGCAAACGGCAGTTTAGCAACGGAAGTCAGAGAGGCTACTGCACCTTTCTGGTCACGACCGTGCATCGGGTTAGCACCCGGTCCGAACGGCGCGCCAGCACGACG  >  Exported/608247‑608384

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑