Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	608,469	G→A	6.0%	Y595Y (TAC→TAT)	pflB ←	pyruvate formate lyase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	608,469	0	G	A	6.0%	248.6 / 3.7	83	Y595Y (TAC→TAT)	pflB	pyruvate formate lyase I
Reads supporting (aligned to +/- strand):  ref base G (37/41);  new base A (3/2);  total (40/43)
Fisher's exact test for biased strand distribution p-value = 6.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.70e-01

ACCCGTTTTCTTACCATACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAACGTTCTACCAGGTCAACAGCCAGGTCATCTACACGCG  >  Exported/608400‑608539                                                                      |                                                                       aCCCGTTTTCTTACCATACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACgg                                                                        >  1:115970/1‑70 (MQ=255)     gTTTTCTTACCATACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACgg                                                                        >  1:3634530/1‑66 (MQ=255)     gTTTTCTTACCATACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACgg                                                                        >  1:1062633/1‑66 (MQ=255)     gTTTTCTTACCATACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGgt                                                                   >  1:4710746/1‑71 (MQ=255)     gTTTTCTTACCATACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGgt                                                                   >  1:2879597/1‑71 (MQ=255)     gTTTTCTTACCATACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGgt                                                                   >  1:2541054/1‑71 (MQ=255)     gTTTTCTGGCCGTACACCACGTTAGAAGTGATAGTCAGAATCGACTGGGTAGGGACGGCGTTGCGATAGgt                                                                   >  1:2102100/1‑71 (MQ=255)        ttCTTACCATACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGgtgt                                                                 <  1:4831545/70‑1 (MQ=255)        ttCTTACCATACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGtgtg                                                                >  1:1910693/1‑71 (MQ=255)             aCCATACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCa                                                              <  1:3568046/68‑1 (MQ=255)             aCCATACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGt                                                            >  1:1920407/1‑70 (MQ=255)             aCCATACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGt                                                            <  1:4449503/70‑1 (MQ=255)              ccATACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATATCGTCACGGTAGGTGTGCAGttt                                                          <  1:82175/71‑1 (MQ=255)              ccATACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAg                                                             <  1:5049511/68‑1 (MQ=255)                aTACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGt                                                                       <  1:3255532/56‑1 (MQ=255)                aTACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCt                                                        >  1:1650878/1‑71 (MQ=255)                   caccacGTTAGAAGTGATAGTCAGAATCGACTGGGTAGGGACGGCGTTGCGATAgg                                                                    >  1:471017/1‑56 (MQ=255)                   cacaACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGaa                                                     >  1:2005261/1‑71 (MQ=255)                      aaCGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAAttt                                                  >  1:932886/1‑71 (MQ=255)                      aaCGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAAttt                                                  >  1:1961911/1‑71 (MQ=255)                      aaCGTTAGAAGTGATGGTCAGAACAGACTCAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAAttt                                                  <  1:1590691/71‑1 (MQ=255)                       aCGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAAttt                                                  >  1:5092046/1‑70 (MQ=255)                        cGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGGTTCTGAATTttc                                                <  1:4513366/71‑1 (MQ=255)                          ttAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAAttt                                                  <  1:1665726/67‑1 (MQ=255)                          ttAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAAttt                                                  <  1:478686/67‑1 (MQ=255)                          ttAGAAGTGATAGTCAGAATCGACTGGGTAGGGACGGCGTTGCGATAGGTTGGCAGCGCTTTAATTttctt                                              <  1:3191103/71‑1 (MQ=255)                            aGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCa                                            >  1:1923857/1‑71 (MQ=255)                            aGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCa                                            >  1:2664794/1‑71 (MQ=255)                            aGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCa                                            >  1:4144598/1‑71 (MQ=255)                            aGAAGTGATAGTCAGAATCGACTGGGTAGGGACGGCGTTGCGATAGGTTGGCAGCGCTTTAATTTTCTTCa                                            >  1:3691980/1‑71 (MQ=255)                             gAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCAt                                           <  1:3737269/71‑1 (MQ=255)                             gAAGTGATAGTCAGAATCGACTGGGTAGGGACGGCGTTGCGATAGGTTGGCAGCGCTTGAATTTTCTTCAt                                           <  1:903448/71‑1 (MQ=255)                                 tGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTttc                                                <  1:1842762/62‑1 (MQ=255)                                 tGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGa                                         <  1:5430284/69‑1 (MQ=255)                                 tGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGa                                         <  1:2555051/69‑1 (MQ=255)                                  gATGGTCAGAACAGACTGAGTCGGGATAGAGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAAc                                      >  1:1607965/1‑71 (MQ=255)                                   aTGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAg                                                                     >  1:1619658/1‑39 (MQ=255)                                   aTGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAAc                                      <  1:5169601/70‑1 (MQ=255)                                   aTGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAAc                                      <  1:2557183/70‑1 (MQ=255)                                   aTGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAACg                                     >  1:4177682/1‑71 (MQ=255)                                   aTGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAACg                                     <  1:411506/71‑1 (MQ=255)                                      gTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGGGTGCAGTTTCTGAATTTTCTTCATGAAACGtt                                   <  1:470384/70‑1 (MQ=255)                                       tCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAACGTTCt                                 <  1:836843/71‑1 (MQ=255)                                        cAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAAttt                                                  >  1:512016/1‑53 (MQ=255)                                        cAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAAttt                                                  >  1:3275993/1‑53 (MQ=255)                                         aGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAACGTTCTa                                >  1:457559/1‑70 (MQ=255)                                          gAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAACGTTCTAcc                              <  1:4320318/71‑1 (MQ=255)                                           aaCAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAAc                                      <  1:727378/62‑1 (MQ=255)                                           aaCAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAAc                                      <  1:4152402/62‑1 (MQ=255)                                           aaCAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAAc                                      <  1:3001829/62‑1 (MQ=255)                                            aCAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAg                                                             >  1:5564359/1‑38 (MQ=255)                                            aCAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTg                                                       >  1:2980204/1‑44 (MQ=255)                                             cAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAACGTTCTACCAgg                           >  1:2523699/1‑71 (MQ=255)                                                aCTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAAc                                      <  1:1551903/57‑1 (MQ=255)                                                aCTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAACGTTCTACCAGGTCa                        <  1:712001/71‑1 (MQ=255)                                                aCTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAACGTTCTACCAGGTCa                        <  1:5237242/71‑1 (MQ=255)                                                aCTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAACGATCTACCAGGTc                         >  1:2196096/1‑70 (MQ=255)                                                 cTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAAttt                                                  <  1:4267765/44‑1 (MQ=255)                                                 cTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAAttt                                                  <  1:4296603/44‑1 (MQ=255)                                                  tGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAAttt                                                  <  1:1389460/43‑1 (MQ=255)                                                      tCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAACGTTCTACCAGGTc                         <  1:2804514/64‑1 (MQ=255)                                                       cGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAAttt                                                  <  1:5526022/38‑1 (MQ=255)                                                       cGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAACGt                                    <  1:3884306/52‑1 (MQ=255)                                                       cGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAACGt                                    <  1:1309733/52‑1 (MQ=255)                                                        gggATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAACGTTGTACCAGGTCAACAGCCAg                >  1:3342243/1‑71 (MQ=255)                                                        gggATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAACGTTCTACCAGGTCAACAGCCAg                <  1:4270063/71‑1 (MQ=255)                                                        gggATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAACGTTCTACCAGGTCAACAGCCAg                >  1:4812152/1‑71 (MQ=255)                                                        gggATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAACGTTCTACCAGGTCAACAGCCAg                >  1:3315199/1‑71 (MQ=255)                                                        gggATAGCGTCAAGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAACGTTCTACCAGGTCAACAGCCAg                >  1:1855319/1‑71 (MQ=255)                                                           aTAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAACGTTCTACCAGGTCAACAGCCAGGTc             <  1:5285724/71‑1 (MQ=255)                                                             aGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAACg                                     >  1:4113556/1‑45 (MQ=255)                                                              gCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAACGTTCTACCAGGTCaa                       >  1:4700207/1‑58 (MQ=255)                                                              gCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAACGTTCTACCAGGTCaa                       >  1:2090656/1‑58 (MQ=255)                                                              gCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAACGTTCTACCAGGTCaa                       >  1:3637429/1‑58 (MQ=255)                                                              gCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAACGTTCTACCAGGTCAACAGCCAGGTCATc          <  1:3679454/71‑1 (MQ=255)                                                               cGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAACGTTCTa                                <  1:2057071/48‑1 (MQ=255)                                                               cGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAACGTTCTACCAGGTCAACAGCCAGGTCAt           <  1:418698/69‑1 (MQ=255)                                                               agTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAACGTTCTa                                <  1:965159/47‑1 (MQ=255)                                                                gTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAACGTTCTACCAGGTCAACAGCCAGGTCATCTa        >  1:3990721/1‑71 (MQ=255)                                                                gTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAACGTTCTACCAGGTCAACAGCCAGGTCATCTa        <  1:2031069/71‑1 (MQ=255)                                                                 tCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAACGTTCTACCAGGTCAACAGCCAGGTCATCTa        >  1:1616694/1‑70 (MQ=255)                                                                  cACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAACGTTCTACCAGGTCAACAGCCAGGTCATCTaca      >  1:2079075/1‑71 (MQ=255)                                                                   aCGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAACGTTCTACCAg                            >  1:4443120/1‑48 (MQ=255)                                                                   aCGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAACGTTCTACCAGGTCAACAGCCAGGTCATCTacac     >  1:4155460/1‑71 (MQ=255)                                                                   aCGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAACGTTCTACCAGGTCAACAGCCAGGTCATCTacac     >  1:4110448/1‑71 (MQ=255)                                                                    cGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAACGTTCTACCAGGTCaa                       <  1:3484473/52‑1 (MQ=255)                                                                      gTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAACGTTCTACCAGGTCAACAGCCAGGTCATCTACAcgcg  >  1:3661614/1‑71 (MQ=255)                                                                      |                                                                       ACCCGTTTTCTTACCATACACAACGTTAGAAGTGATGGTCAGAACAGACTGAGTCGGGATAGCGTCACGGTAGGTGTGCAGTTTCTGAATTTTCTTCATGAAACGTTCTACCAGGTCAACAGCCAGGTCATCTACACGCG  >  Exported/608400‑608539

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑