Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	608,670	A→C	5.8%	A528A (GCT→GCG)	pflB ←	pyruvate formate lyase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	608,670	0	A	C	5.8%	227.6 / 4.1	85	A528A (GCT→GCG)	pflB	pyruvate formate lyase I
Reads supporting (aligned to +/- strand):  ref base A (46/34);  new base C (3/2);  total (49/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

TTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTG  >  Exported/608601‑608739                                                                      |                                                                       ttCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAg                                                                       >  1:2221473/1‑71 (MQ=255) ttCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAg                                                                       >  1:5559560/1‑71 (MQ=255)   cGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGc                                                                      <  1:525254/70‑1 (MQ=255)   cGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGc                                                                      <  1:5559538/70‑1 (MQ=255)   cGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGc                                                                      <  1:829012/70‑1 (MQ=255)    gTCACGGATTGGTTTCACGCGGGCATATTTGATGGCAGACAGGGAGTCCGTCGCCACCGACAGGCCCGc                                                                      <  1:5654942/69‑1 (MQ=255)     tCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGAt                                                                   <  1:4089282/71‑1 (MQ=255)       aCGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATaa                                                                 >  1:4233397/1‑70 (MQ=255)       aCGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATAc                                                                 >  1:1333560/1‑71 (MQ=255)         gAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACca                                                               <  1:2480866/71‑1 (MQ=255)         gAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACca                                                               <  1:463864/71‑1 (MQ=255)           aTCGGTTTAACTTTCGCATATTTTATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACcaca                                                             <  1:3384186/71‑1 (MQ=255)           aTCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACcaca                                                             <  1:2769197/71‑1 (MQ=255)            tCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCacac                                                            >  1:5277265/1‑71 (MQ=255)               gTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGcc                                                         <  1:1521978/71‑1 (MQ=255)               gTTAAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGc                                                          <  1:3387994/70‑1 (MQ=255)                 ttAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCAt                                                       >  1:5109093/1‑71 (MQ=255)                  tAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATg                                                      >  1:3085328/1‑71 (MQ=255)                  tAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATg                                                      >  1:115620/1‑71 (MQ=255)                  tAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATg                                                      >  1:5075723/1‑71 (MQ=255)                   aaCTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATg                                                      >  1:2842431/1‑70 (MQ=255)                     cTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCa                                                                        <  1:4223321/50‑1 (MQ=255)                     cTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCa                                                                        <  1:38620/50‑1 (MQ=255)                     cTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCa                                                                        <  1:758341/50‑1 (MQ=255)                     cTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCa                                                                        <  1:4423978/50‑1 (MQ=255)                     cTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTg                                                   <  1:117589/71‑1 (MQ=255)                      tttCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGc                                                  >  1:2713393/1‑71 (MQ=255)                       ttCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTg                                                   >  1:5359604/1‑69 (MQ=255)                       ttCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCg                                                 >  1:4107728/1‑71 (MQ=255)                        tCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGc                                                  <  1:2585078/69‑1 (MQ=255)                        tCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGc                                                  <  1:2390300/69‑1 (MQ=255)                        gggCATATTTGATGGCAGACAGGGAGTCCGTCGCCACCGACAGGCCCGCGATGCCGCATGCCATAGTGCga                                                <  1:373464/69‑2 (MQ=255)                         cGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATgg                                                     >  1:1881742/1‑65 (MQ=255)                         cGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATgg                                                     >  1:1824049/1‑65 (MQ=255)                          gCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGAt                                              <  1:937516/71‑1 (MQ=255)                           cttatTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATa                                             <  1:2431491/69‑1 (MQ=255)                           cATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATa                                             <  1:920176/71‑1 (MQ=255)                           cATATTTGATGGCAGACAGGGAGTCCGTCGCCACCGACAGGCCCGCGa                                                                    >  1:1089330/1‑48 (MQ=255)                             tatTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGAt                                                                   <  1:1127564/47‑1 (MQ=255)                              atTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGc                                                                      <  1:574690/43‑1 (MQ=255)                              atTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACg                                         >  1:1149481/1‑71 (MQ=255)                               tttGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGa                                                                    <  1:218253/44‑1 (MQ=255)                               tttGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGt                                        >  1:4624953/1‑71 (MQ=255)                               tttGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGt                                        >  1:4120457/1‑71 (MQ=255)                               tttGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGt                                        >  1:3464094/1‑71 (MQ=255)                               tttGATGGCAGACAGGGAGTCCGTCGCCACCGACAGGCCCGc                                                                      >  1:1975401/1‑42 (MQ=255)                                ttGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGt                                        >  1:3549713/1‑70 (MQ=255)                                ttGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGt                                        >  1:4482926/1‑70 (MQ=255)                                 tGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTc                                       <  1:4495706/70‑1 (MQ=255)                                 tGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACACCATGGTGCGGATA‑ACGTCa                                      >  1:1826472/1‑71 (MQ=255)                                    ttGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACgg                                   <  1:709857/71‑1 (MQ=255)                                     tGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGt                                  >  1:3881570/1‑71 (MQ=255)                                      gCAGACAGGGAGTCAGCAGCGACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTc                                 >  1:1409918/1‑71 (MQ=255)                                      gCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATa‑a                                           >  1:2960014/1‑61 (MQ=255)                                      gCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATa‑a                                           >  1:5666001/1‑61 (MQ=255)                                      gCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTc                                 >  1:4431275/1‑71 (MQ=255)                                      gCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTc                                 >  1:5365467/1‑71 (MQ=255)                                      gCAGACAGGGAGTCAGCAGCAACGGACAGAACAGCGATACCACACGCCATGGTGCGGATa‑a                                           >  1:898897/1‑61 (MQ=255)                                       cAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTc                                 <  1:70206/70‑1 (MQ=255)                                       cAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCg                                >  1:3893114/1‑71 (MQ=255)                                         gACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCgcg                              <  1:994016/71‑3 (MQ=255)                                         gACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTg                              <  1:2664318/71‑1 (MQ=255)                                          aCAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTg                              >  1:4380746/1‑70 (MQ=255)                                          aCAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGc                             <  1:259841/71‑1 (MQ=255)                                           cAGGGAGTCAGCAGCACCGGACAGACCAGCGATACcaca                                                             >  1:4979632/1‑39 (MQ=255)                                           cAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGc                             <  1:5063168/70‑1 (MQ=255)                                           cAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCa                            <  1:2870297/71‑1 (MQ=255)                                            aGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAg                           >  1:4929491/1‑71 (MQ=255)                                              ggAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCa                            >  1:4325930/1‑68 (MQ=255)                                              ggAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCa                            >  1:767944/1‑68 (MQ=255)                                                  tCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCAt                     <  1:5488704/71‑1 (MQ=255)                                                    agcagcAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAg                           >  1:4966893/1‑63 (MQ=255)                                                    agcagcAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCAt                     >  1:3859894/1‑69 (MQ=255)                                                    agcagcAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCAt                     >  1:2339797/1‑69 (MQ=255)                                                    agcagcAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCa                   <  1:5218487/71‑1 (MQ=255)                                                         caACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑Ac                                          <  1:1283199/43‑1 (MQ=255)                                                          aaCGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑Ac                                          <  1:1612146/42‑1 (MQ=255)                                                           aCGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCagaga               >  1:5597367/1‑68 (MQ=255)                                                           aCGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCagaga               >  1:606469/1‑68 (MQ=255)                                                           aCGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGc            >  1:225641/1‑71 (MQ=255)                                                            ccgACAGGCCCGCGATGCCGCATGCCATAGTGC‑GATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCt           >  1:2536424/3‑71 (MQ=255)                                                             ggACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCtt          <  1:203083/71‑1 (MQ=255)                                                               aCAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCg        >  1:190803/1‑71 (MQ=255)                                                                     cAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCtt          >  1:4804471/1‑63 (MQ=255)                                                                     cAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCtt          >  1:4797998/1‑63 (MQ=255)                                                                     cAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCtt          >  1:449662/1‑63 (MQ=255)                                                                     cAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCtt          >  1:789345/1‑63 (MQ=255)                                                                     cAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTg  <  1:4899071/71‑1 (MQ=255)                                                                     cAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGGCGTGCAGCGCCAt                     <  1:4551539/52‑1 (MQ=255)                                                                      |                                                                       TTCGTCACGAATCGGTTTAACTTTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTG  >  Exported/608601‑608739

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑