Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 189,433 C→T 15.7% G310G (GGC→GGT ldcC → lysine decarboxylase 2, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported189,4330CT15.7% 209.9 / 25.8 89G310G (GGC→GGTldcClysine decarboxylase 2, constitutive
Reads supporting (aligned to +/- strand):  ref base C (45/30);  new base T (5/9);  total (50/39)
Fisher's exact test for biased strand distribution p-value = 1.41e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

GAAAGTCGCTGCTACCACGCAAGCACAATGGCCGGTTCATGCGGTGATCACCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTG  >  Exported/189364‑189501
                                                                     |                                                                    
gaAAGTCGCTGCTACCACGCAAGCACAATGGCCGGTTCATGCGGTGATCACCAACTCCACCTATGATGGCt                                                                     >  1:3894315/1‑71 (MQ=255)
gaAAGTCGCTGCTACCACGCAAGCACAATGGCCGGTTCATGCGGTGATCACCAACTCCACCTATGATGGCt                                                                     >  1:4623857/1‑71 (MQ=255)
   aGTCGCTGCTACCACGCAAGCACAATGGCCGGTTCATGCGGTGATCACCAACTCCACCTATGATAGCtcg                                                                   >  1:5003596/1‑68 (MQ=255)
      cGCTGCTACCACGCAAGCACAATGGCCGGTTCATGCGGTGATCACCAACTCCACCTATGATGGCTTGctc                                                                >  1:854265/1‑70 (MQ=255)
      cGCTGCTACCACGCAAGCACAATGGCCGGTTCATGCGGTGATCACCAACTCCACCTATGATGGCTTGctc                                                                >  1:269261/1‑70 (MQ=255)
              ccACGCAAGCACAATGGCCGGTTCATGCGGTGATCACCAACTCCACCTATGATGGCTTGCTCTacaaca                                                         <  1:2754691/69‑1 (MQ=255)
                 cGCAAGCACAATGGCCGGTTCATGCGGTGATCACCAACTCCACCTATGATGGCTTGCTCTACAACACCGAc                                                    <  1:3266972/71‑1 (MQ=255)
                   cAAGCACAATGGCCGGTTCATGCGGTGATCACCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTg                                                  <  1:3064601/71‑1 (MQ=255)
                     aGCACAATGGCCGGTTCATGCGGTGATCACCAACTCCACCTATGATGGCTTg                                                                   <  1:4143261/52‑1 (MQ=255)
                     aGCACAATGGCCGGTTCATGCGGTGATCACCAACTCCACCTATGATGGCTTg                                                                   <  1:2624873/52‑1 (MQ=255)
                       cacaATGGCCGGTTCATGCGGTGATCACCAACTCCGCCTATGATGGCTTGct                                                                 >  1:2516591/1‑52 (MQ=255)
                       cacaATGGCCGGTTCATGCGGTGATCACCAACTCCACCTATGATGGCTTGCTCTacaa                                                           >  1:1040026/1‑58 (MQ=255)
                        acaATGGCCGGTTCATGCGGTGATCACCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCa                                             <  1:4239061/71‑1 (MQ=255)
                          cctGGCCGGTACATGCTGTAATTACCAACTCTACCTATGATGGTCTGCTGTACAACACCGa                                                     <  1:4758161/59‑1 (MQ=255)
                          cctGGCCGGTACATGCTGTAATTACCAACTCTACCTATGATGGTCTGCTGTACAACACCGACTTCATCaag                                           <  1:2019451/69‑2 (MQ=255)
                           ctGGCCGGTACATGCTGTAATTACCAACTCTACCTATGATGGTCTGCTGTACAACACCGa                                                     <  1:5471160/59‑1 (MQ=255)
                           ctGGCCGGTACATGCTGTAATTACCAACTCTACCTATGATGGTCTGCTGTACAACACCGACTTCATCaaga                                          <  1:471353/70‑3 (MQ=255)
                           aTGGCCGGTTCATGCGGTGATCACCAACTCCACCTATGATGGCTTGCTCTac                                                             <  1:2466371/52‑1 (MQ=255)
                              gCCGGTACATGCTGTAATTACCAACTCTACCTATGATGGTCTGCTGTACAACACCGACTTCATCaagaa                                         <  1:2937832/69‑4 (MQ=255)
                               ccGGTTCATGCGGTGATCACCAACTCCACCTATGATGGCTTGCTGTac                                                             >  1:5620116/1‑48 (MQ=255)
                               ccGGTTCATGCGGTGATCACCAACTCCACCTATGATGGCTTGCTCTac                                                             >  1:5037705/1‑48 (MQ=255)
                               ccGGTTCATGCGGTGATCACCAACTCCACCTATGATGGCTTGCTCTac                                                             >  1:2866530/1‑48 (MQ=255)
                               ccGGTTCATGCGGTGATCACCAACTCCACCTATGATGGCTTGCTCTac                                                             >  1:2705220/1‑48 (MQ=255)
                               ccGGTTCATGCGGTGATCACCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGAc                                      >  1:1621106/1‑71 (MQ=255)
                                 ggTTCATGCGGTGATCACCAACTCCACCTATGATGGCTTg                                                                   <  1:2316454/40‑1 (MQ=255)
                                 ggTTCATGCGGTGATCACCAACTCCACCTATGATGGCTTGCTCTACAACACCGAc                                                    >  1:799590/1‑55 (MQ=255)
                                 ggTTCATGCGGTGATCACCAACTCCACCTATGATGGCTTGCTCTACAACACCGAc                                                    >  1:4322079/1‑55 (MQ=255)
                                   ttCATGCGGTGATCACCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTg                                  >  1:338588/1‑71 (MQ=255)
                                    tCATGCGGTGATCACCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTg                                  >  1:4337910/1‑70 (MQ=255)
                                     cATGCTGTAATTACCAACTCTACCTATGATGGTCTGCTGTACAACACCGACTTCATCAAGAAAACACTGGa                                >  1:5507862/1‑71 (MQ=255)
                                     cATGCTGTAATTACCAACTCTACCTATGATGGTCTGCTGTACAACACCGACTTCATCAAGAAAACACTGGa                                <  1:1329826/71‑1 (MQ=255)
                                     cATGCTGTAATTACCAACTCTACCTATGATGGTCTGCTGTACAACACCGACTTCATCAAGAAAACACTGGa                                <  1:5363489/71‑1 (MQ=255)
                                     cATGCGGTGATCACCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTg                                                  >  1:5636815/1‑53 (MQ=255)
                                     cATGCGGTGATCACCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTg                                                  >  1:4641445/1‑53 (MQ=255)
                                     cATGCGGTGATCACCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGa                                >  1:4167162/1‑71 (MQ=255)
                                         cGGTGATCACCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCtt                                  <  1:1216458/65‑2 (MQ=255)
                                            tGATCACCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCg                         >  1:4888144/1‑71 (MQ=255)
                                            tGATCACCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCg                         >  1:5152287/1‑71 (MQ=255)
                                              aTCACCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCa                                             <  1:481164/49‑1 (MQ=255)
                                              aTCACCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCa                                             <  1:1169489/49‑1 (MQ=255)
                                              aTCACCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCa                                             <  1:5248894/49‑1 (MQ=255)
                                              aTCACCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCa                                             <  1:2425624/49‑1 (MQ=255)
                                              aTCACCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTc                       <  1:2132984/71‑1 (MQ=255)
                                                 aCCAACTCTACCTATGATGGTCTGCTGTTCAACAcc                                                       >  1:2196565/1‑36 (MQ=16)
                                                 aCCAACTCTACCTATGATGGTCTGCTGTACAACAcc                                                       >  1:5656684/1‑36 (MQ=19)
                                                 aCCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTg                                  <  1:4767132/57‑1 (MQ=255)
                                                 aCCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTg                                  <  1:1774295/57‑1 (MQ=255)
                                                 aCCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTg                                  <  1:4434977/57‑1 (MQ=255)
                                                 aCCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACCCTg                                  <  1:2794207/57‑1 (MQ=255)
                                                  ccAACTCCACCTATGATGGCTTGCTCTACAACACCGACTTGATCAAACAGACGCTGGATGTCCCGTCGAt                    >  1:2398691/1‑70 (MQ=255)
                                                  ccAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGAt                    >  1:3215029/1‑70 (MQ=255)
                                                   cAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTgg                                 <  1:1753475/56‑1 (MQ=255)
                                                   cAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTc                       >  1:4768275/1‑66 (MQ=255)
                                                   cAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTc                       >  1:4245769/1‑66 (MQ=255)
                                                   cAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTc                       >  1:4336822/1‑66 (MQ=255)
                                                    aaCTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATg                              <  1:4415161/58‑1 (MQ=255)
                                                     aCTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTc                       >  1:5210805/1‑64 (MQ=255)
                                                     aCTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCAc                >  1:2508859/1‑71 (MQ=255)
                                                     aCTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCAc                >  1:4155830/1‑71 (MQ=255)
                                                      ctctACCTATGATGGTCTGCTGTACAACACCGACTTCATCAAGAAAACACTGGATg                              <  1:2685241/56‑1 (MQ=255)
                                                        ctaCCTATGATGGTCTGCTGTACAACACCGACTTCATCAAGAAAACACTGGa                                >  1:647599/3‑52 (MQ=255)
                                                        ccACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGa                                >  1:4783054/1‑52 (MQ=255)
                                                        ccACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGa                                >  1:2292827/1‑52 (MQ=255)
                                                        ccACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGa                                >  1:4345823/1‑52 (MQ=255)
                                                        ccACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGa                                >  1:3638267/1‑52 (MQ=255)
                                                        ccACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGa                                >  1:4280845/1‑52 (MQ=255)
                                                        ccACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTc             >  1:4535807/1‑71 (MQ=255)
                                                          aCCTATGATGTCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGa           <  1:1400329/71‑1 (MQ=255)
                                                          aCCTATGATGGTCTGCTGTACAACACCGACTTCATCAAGAAAACACTGGa                                >  1:3096392/1‑50 (MQ=255)
                                                          aCCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGa                                       >  1:3073922/1‑43 (MQ=255)
                                                          aCCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTg                                  >  1:4819432/1‑48 (MQ=255)
                                                          aCCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGa           <  1:4100464/71‑1 (MQ=255)
                                                          aCCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGa           <  1:3830497/71‑1 (MQ=255)
                                                           ccTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTg                                  <  1:5408528/47‑1 (MQ=255)
                                                            cTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTgg                                 <  1:5400529/47‑1 (MQ=255)
                                                            cTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCg            <  1:2277606/68‑1 (MQ=255)
                                                             tATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGa                     >  1:2875733/1‑58 (MQ=255)
                                                             tATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGa                     >  1:1831980/1‑58 (MQ=255)
                                                             tATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGa                     >  1:5093710/1‑58 (MQ=255)
                                                             tATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGa                     >  1:417307/1‑58 (MQ=255)
                                                             tATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGa                     >  1:5669044/1‑58 (MQ=255)
                                                              atgatgGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGAt          >  1:4954634/1‑68 (MQ=255)
                                                               tgatgGTCTGCTGTACAACACCGACTTCATCAAGAAAACACTGGATGtga                           <  1:3746880/50‑3 (MQ=255)
                                                               tgatgGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTg                                  <  1:5089149/43‑1 (MQ=255)
                                                               tgatgGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTc             <  1:4663397/64‑1 (MQ=255)
                                                               tgatgGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTg      >  1:5642568/1‑71 (MQ=255)
                                                                 atgGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGcc    >  1:1373105/1‑71 (MQ=255)
                                                                  tgGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCt   <  1:4418760/71‑1 (MQ=255)
                                                                    gCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTg  <  1:614753/70‑1 (MQ=255)
                                                                     |                                                                    
GAAAGTCGCTGCTACCACGCAAGCACAATGGCCGGTTCATGCGGTGATCACCAACTCCACCTATGATGGCTTGCTCTACAACACCGACTGGATCAAACAGACGCTGGATGTCCCGTCGATTCACTTCGATTCTGCCTG  >  Exported/189364‑189501

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: