| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 608,790 | A→C | 23.7% | A488A (GCT→GCG) | pflB ← | pyruvate formate lyase I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 608,790 | 0 | A | C | 23.7% | 199.7 / 90.8 | 135 | A488A (GCT→GCG) | pflB | pyruvate formate lyase I |
| Reads supporting (aligned to +/- strand): ref base A (57/46); new base C (13/19); total (70/65) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.61e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01 | |||||||||||
Only 100 of 163 total aligned reads displayed.
CAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAGGACATCGCCTTTGATC > Exported/608721‑608859 | cAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTag > 1:1534630/1‑71 (MQ=255) agagagGCTTCGTAGCTGTACTTGTCGAGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTagc > 1:3157256/1‑71 (MQ=255) tCGTAGCTGTACTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTc > 1:4167962/1‑71 (MQ=255) cGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTc < 1:2279433/70‑1 (MQ=255) agatGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGGCAGCCAGTCCAt > 1:2373097/4‑70 (MQ=255) gCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTagccagc < 1:824590/61‑1 (MQ=255) gCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTagccagc < 1:5332163/61‑1 (MQ=255) gCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATg > 1:4530813/1‑70 (MQ=255) gCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATg > 1:4338760/1‑70 (MQ=255) gCTGTACTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATg < 1:1020106/70‑1 (MQ=255) cTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATg > 1:3284711/1‑69 (MQ=255) gTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGt > 1:4273800/1‑71 (MQ=255) gTACTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGt > 1:1201001/1‑71 (MQ=255) tACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTagc < 1:2415906/53‑1 (MQ=255) tACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTagc < 1:565187/53‑1 (MQ=255) tACTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTg < 1:4662897/71‑1 (MQ=255) tACTTGTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTg < 1:5146292/71‑1 (MQ=255) aCTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGa > 1:538167/1‑71 (MQ=255) aCTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGa > 1:5543885/1‑71 (MQ=255) aCTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGa > 1:4961221/1‑71 (MQ=255) cTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTagc < 1:2815589/51‑1 (MQ=255) cTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTagc < 1:4455116/51‑1 (MQ=255) cTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGAt < 1:80889/71‑1 (MQ=255) cTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGAt < 1:628207/71‑1 (MQ=255) ttGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTagcca < 1:3823966/52‑1 (MQ=255) ttGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATc > 1:5420974/1‑71 (MQ=255) tGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTTATGTACTGTTTagcca > 1:4660148/1‑51 (MQ=255) tGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTagcca > 1:4194749/1‑51 (MQ=255) tGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGAt > 1:3390186/1‑69 (MQ=255) tGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGAt > 1:3428816/1‑69 (MQ=255) tGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGAt > 1:2211188/1‑69 (MQ=255) tGTCGTGCATGTAGTGGATGATGTTCACTGCAGTGATGTACTGTTTagcca > 1:395611/1‑51 (MQ=255) gTCTTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATc < 1:1123840/69‑1 (MQ=255) gTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATc < 1:2985682/69‑1 (MQ=255) gTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATc < 1:5453829/69‑1 (MQ=255) gTCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATc < 1:2454362/69‑1 (MQ=255) tCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATc < 1:3352880/68‑1 (MQ=255) tCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCAt > 1:4930906/1‑71 (MQ=255) tCGTGCATGTAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATcga < 1:2636195/70‑3 (MQ=255) cGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATg > 1:5019723/1‑71 (MQ=255) gCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTagccagcc < 1:1966901/48‑1 (MQ=255) cATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATgcgc < 1:35490/70‑1 (MQ=255) cATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCt > 1:5178894/1‑71 (MQ=255) tGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTagccagc < 1:919093/44‑1 (MQ=255) tGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTagcaagc < 1:558436/44‑1 (MQ=255) tGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATg < 1:3589835/53‑1 (MQ=255) tGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTc > 1:4003165/1‑70 (MQ=255) tGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTc > 1:3015956/1‑70 (MQ=255) tAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCAt < 1:4527083/71‑1 (MQ=255) tAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGaa > 1:770928/1‑53 (MQ=12) tAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGaa > 1:5577554/1‑53 (MQ=12) tAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGaa > 1:1852218/1‑53 (MQ=12) tAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATc < 1:1008395/59‑1 (MQ=255) tAGTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATc < 1:1843464/59‑1 (MQ=255) gTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAg < 1:195627/52‑1 (MQ=12) tGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGa < 1:879306/49‑1 (MQ=255) tGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGa < 1:5271029/49‑1 (MQ=255) tGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCAc < 1:954640/71‑1 (MQ=255) tGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCAc < 1:2240940/71‑1 (MQ=255) gatgatGTTCAGTGCAGTGATGTACTGTTTagccag < 1:3014893/36‑1 (MQ=255) gatgatGTTCAGTGCAGTGATGTACTGTTTagccag < 1:921637/36‑1 (MQ=255) gatgatGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAg > 1:3481864/1‑49 (MQ=255) gatgatGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGAt > 1:4689700/1‑53 (MQ=255) gatgatGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACt < 1:2768294/70‑1 (MQ=255) aatgataTTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACtt > 1:1423752/2‑71 (MQ=255) atgataTTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACttt > 1:4764583/1‑70 (MQ=255) tgataTTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACtttg < 1:2684964/71‑3 (MQ=255) tgataTTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACtttg < 1:1182116/71‑3 (MQ=255) atgTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATcc > 1:4782532/1‑51 (MQ=255) tgTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATg < 1:5018171/41‑1 (MQ=255) ttCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTtcatca > 1:5059907/1‑69 (MQ=255) ttCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCa < 1:5063728/61‑1 (MQ=255) tCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTtcat > 1:1206162/1‑66 (MQ=255) tCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAg > 1:1383646/1‑71 (MQ=255) tCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAg > 1:4597266/1‑71 (MQ=255) cagtgcagtgATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAg > 1:528916/1‑70 (MQ=255) gtgcagtgATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTc < 1:1142408/71‑1 (MQ=255) gtgcagtgATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTc < 1:3086584/71‑1 (MQ=255) gtgcagtgATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTc < 1:5513822/71‑1 (MQ=255) tgcagtgATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATcc < 1:660559/43‑1 (MQ=255) agtgATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAgg < 1:3987384/70‑1 (MQ=255) gtgATGTACTGTTTAGCCAGCCAGTCCATGAAGTGa > 1:1914363/1‑36 (MQ=255) gtgATGTACTGTTTAGCCAGCCAGTCCATGAAGTGAt > 1:1336284/1‑37 (MQ=255) gtgATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATcc < 1:2905721/39‑1 (MQ=255) gtgATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACtt < 1:4432361/55‑1 (MQ=255) gtgATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAGGAc < 1:2126794/71‑1 (MQ=255) tgATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCa < 1:2086920/66‑1 (MQ=255) tgATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAGGAc < 1:1278528/70‑1 (MQ=255) tgATGTACTGCACCGCCAGCCAGTCCATGAAGTGATc < 1:4753590/37‑1 (MQ=21) tgATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCa < 1:5043685/51‑1 (MQ=255) tACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCa < 1:4835358/43‑1 (MQ=255) tGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAGGACATCGCCttt > 1:687099/1‑71 (MQ=255) gTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCa > 1:524488/1‑39 (MQ=255) gTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCa > 1:4808103/1‑39 (MQ=255) gTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCa > 1:1775835/1‑39 (MQ=255) gTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAGGACATCGCCttt > 1:610017/1‑70 (MQ=255) tAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCa > 1:2599519/1‑39 (MQ=255) tAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAGGACATCGCCTTTGATc > 1:2921449/1‑71 (MQ=255) tAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAGGACATCGCCTTTGATc > 1:5209155/1‑71 (MQ=255) tAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAGGACATCGCCTTTGATc > 1:2914802/1‑71 (MQ=255) | CAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAGGACATCGCCTTTGATC > Exported/608721‑608859 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |