BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	608,814	C→G	9.9%	M480I (ATG→ATC) ‡	pflB ←	pyruvate formate lyase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	608,814	0	C	G	9.9%	280.7 / 17.0	101	M480I (ATG→ATC) ‡	pflB	pyruvate formate lyase I
Reads supporting (aligned to +/- strand): ref base C (53/38); new base G (4/6); total (57/44)
Fisher's exact test for biased strand distribution p-value = 3.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.73e-01

Only 100 of 108 total aligned reads displayed.

GTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAGGACATCGCCTTTGATCGGTTCAGACTTCGGACCAACCTG  >  Exported/608745‑608882

gTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATcc                                                                      <  1:2236535/70‑1 (MQ=255)

gTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCa                                                                     >  1:3310956/1‑71 (MQ=255)

 tCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCAt                                                                    >  1:4930906/1‑71 (MQ=255)

  cGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATg                                                                   >  1:5374400/1‑71 (MQ=255)

  cGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATg                                                                   >  1:5019723/1‑71 (MQ=255)

  cGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATg                                                                   >  1:5019602/1‑71 (MQ=255)

      cATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATgcgc                                                                <  1:35490/70‑1 (MQ=255)

      cATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCt                                                               >  1:5178894/1‑71 (MQ=255)

      cATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCt                                                               <  1:3455623/71‑1 (MQ=255)

        tGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTc                                                              >  1:738708/1‑70 (MQ=255)

        tGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTc                                                              >  1:3015956/1‑70 (MQ=255)

        tGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTc                                                              >  1:4003165/1‑70 (MQ=255)

          tAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCa                                                            <  1:4580983/70‑1 (MQ=255)

          tAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCAt                                                           <  1:4527083/71‑1 (MQ=255)

            gTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCa                                                         <  1:2696829/71‑1 (MQ=255)

            gTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCa                                                         <  1:3008225/71‑1 (MQ=255)

            gTGAATGATATTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCa                                                         >  1:2323210/1‑71 (MQ=255)

             tGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCAc                                                        <  1:2240940/71‑1 (MQ=255)

             tGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCAc                                                        <  1:954640/71‑1 (MQ=255)

               gatgatGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACt                                                       <  1:2768294/70‑1 (MQ=255)

               aatgataTTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACtt                                                      >  1:1423752/2‑71 (MQ=255)

                atgataTTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACttt                                                     >  1:4764583/1‑70 (MQ=255)

                atgataTTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACttt                                                     <  1:4779975/71‑2 (MQ=255)

                 tgataTTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACtttg                                                    <  1:2684964/71‑3 (MQ=255)

                 tgataTTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACtttg                                                    >  1:3939805/1‑69 (MQ=255)

                 tgataTTCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCACtttg                                                    <  1:1182116/71‑3 (MQ=255)

                   atgTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATcc                                                                      >  1:4782532/1‑51 (MQ=255)

                    tgTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTtcatca                                                 <  1:1855108/71‑1 (MQ=255)

                      ttCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTtcatca                                                 >  1:4852286/1‑69 (MQ=255)

                      ttCAGCGCGCTGATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCa                                                         <  1:5063728/61‑1 (MQ=255)

                       tCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTtcat                                                   >  1:1206162/1‑66 (MQ=255)

                       tCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAg                                              >  1:1383646/1‑71 (MQ=255)

                       tCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAg                                              >  1:4597266/1‑71 (MQ=255)

                        cagtgcagtgATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAg                                              >  1:2147734/1‑70 (MQ=255)

                        cagtgcagtgATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAg                                              >  1:528916/1‑70 (MQ=255)

                        cagtgcagtgATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAg                                              >  1:5662568/1‑70 (MQ=255)

                        cagtgcagtgATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGt                                             >  1:87301/1‑71 (MQ=255)

                          gtgcagtgATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTc                                           <  1:1142408/71‑1 (MQ=255)

                          gtgcagtgATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTc                                           <  1:5513822/71‑1 (MQ=255)

                          gtgcagtgATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTc                                           <  1:3086584/71‑1 (MQ=255)

                           tgcagtgATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATcc                                                                      <  1:660559/43‑1 (MQ=255)

                             cagtgATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAg                                         <  1:3585521/70‑1 (MQ=255)

                             cagtgATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAg                                         <  1:5444385/70‑1 (MQ=255)

                              agtgATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAgg                                        <  1:3987384/70‑1 (MQ=255)

                               gtgATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATcc                                                                      <  1:2905721/39‑1 (MQ=255)

                               gtgATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACtt                                                      <  1:4432361/55‑1 (MQ=255)

                               gtgATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAGGAc                                      <  1:5240645/71‑1 (MQ=255)

                               gtgATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAGGAc                                      <  1:2126794/71‑1 (MQ=255)

                                tgATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCa                                          <  1:2086920/66‑1 (MQ=255)

                                tgATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAGGAc                                      <  1:1278528/70‑1 (MQ=255)

                                tgATGTACTGCACCGCCAGCCAGTCCATGAAGTGATCGAGGCTGTCCATCa                                                         <  1:5043685/51‑1 (MQ=255)

                                  aTGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAg                                         >  1:3079151/1‑65 (MQ=255)

                                     tACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCa                                                            <  1:4835358/43‑1 (MQ=255)

                                       cTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAGGACATCGCCtt                              >  1:1035317/1‑71 (MQ=255)

                                       cTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAGGACATCGCCtt                              >  1:295020/1‑71 (MQ=255)

                                        tGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAGGACATCGCCttt                             >  1:687099/1‑71 (MQ=255)

                                         gTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCa                                                            >  1:1775835/1‑39 (MQ=255)

                                         gTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCa                                                            >  1:524488/1‑39 (MQ=255)

                                         gTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCa                                                            >  1:4808103/1‑39 (MQ=255)

                                         gTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAGGACATCGCCttt                             >  1:610017/1‑70 (MQ=255)

                                            tAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCa                                                         >  1:2599519/1‑39 (MQ=255)

                                            tAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCa                                                         >  1:1327243/1‑39 (MQ=255)

                                            tAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAGGACATCGCCTTTGATc                         >  1:5209155/1‑71 (MQ=255)

                                            tAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAGGACATCGCCTTTGATc                         >  1:2914802/1‑71 (MQ=255)

                                            tAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAGGACATCGCCTTTGATc                         >  1:2921449/1‑71 (MQ=255)

                                              gccagccaGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAGGACATCGCCTTTGATCgg                       <  1:772482/71‑1 (MQ=255)

                                              gccagccaGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAGGACATCGCCTTTGATCgg                       <  1:4783903/71‑1 (MQ=255)

                                               ccagccagTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAGGACATCGCCTTTGATCGGt                      >  1:3239341/1‑71 (MQ=255)

                                                cagccagTCCATGAAGTGATCCATGCGCTACATCACTTCATCATAGTTCAGGACATCGCCTTTGATCGGtt                     <  1:1554525/71‑1 (MQ=255)

                                                  gccagTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAGGACATCGCCTTTGATCGGtt                     >  1:5283357/1‑69 (MQ=255)

                                                  gccagTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAGGACATCGCCTTTGATCGGTTCa                   <  1:4025166/71‑1 (MQ=255)

                                                   ccagTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAGGACATCGCCTTTGATCGGtt                     >  1:1093147/1‑68 (MQ=255)

                                                   ccagTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAGGACATCGCCTTTGATCGGtt                     >  1:658166/1‑68 (MQ=255)

                                                    cagTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAGGACATCGCCTTTGATCGGTTCAGa                 >  1:3063933/1‑71 (MQ=255)

                                                      gTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAGGACATCGCCTTTg                            <  1:3393067/58‑1 (MQ=255)

                                                      gTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAGGACATCGCCTTTGATCGGTTCAGAc                >  1:1429563/1‑70 (MQ=255)

                                                         cATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAGGACATCGCCTTTGATCGGTTCAGACTTCg            >  1:3291293/1‑71 (MQ=255)

                                                         cATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAGGACATCGCCTTTGATCGGTTCAGACTTCg            >  1:5658349/1‑71 (MQ=255)

                                                         cATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAGGACATCGCCTTTGATCGGTTCAGACTTCg            >  1:2315182/1‑71 (MQ=255)

                                                           tGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAGGACATCGCCTTTGAt                          <  1:5092448/55‑1 (MQ=255)

                                                           tGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAGGACATCGCCTTTGATCGGTTCAGACTTCGGa          >  1:2971293/1‑71 (MQ=255)

                                                           tGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAGGACATCGCCTTTGATCGGTTCAGACTTCGGa          >  1:3450673/1‑71 (MQ=255)

                                                            gAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAGGAc                                      >  1:4064017/1‑42 (MQ=255)

                                                            gAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAGGACATCGCCTTTGATCgg                       >  1:3950326/1‑57 (MQ=255)

                                                            gAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAGGACATCGCCTTTGATCGGTTCAGACTTCg            >  1:4070583/1‑68 (MQ=255)

                                                            gAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAGGACATCGCCTTTGATCGGTTCAGACTTCGGAc         >  1:621988/1‑71 (MQ=255)

                                                              aGTGATCCATGCGCTCCATCACTTCATCATAGTTCAGGACATCGCCTTTGATCGGTTCAGACTTc             >  1:258179/1‑65 (MQ=255)

                                                               gTGATCCATGGGCTCCATCACTTCATCATAGTTCAGGACATCGCCTTTGATCGGTTCAGACTTCGGACCaa      >  1:2444895/1‑71 (MQ=255)

                                                               gTGATCCATGCGCTCCATCACTTCATCATAGTTCAg                                         <  1:4025868/36‑1 (MQ=255)

                                                               gTGATCCATGCGCTCCATCACTTCATCATAGTTCAg                                         <  1:4032457/36‑1 (MQ=255)

                                                               gTGATCCATGCGCTCCATCACTTCATCATAGTTCAGGACATCGCCTTTGATCGGTTCAGACTTCGGACCaa      >  1:5097586/1‑71 (MQ=255)

                                                                tGATCCATGCGCTCCATCACTTCATCATAGTTCAGGACATCGCCTTTg                            <  1:1825032/48‑1 (MQ=255)

                                                                tGATCCATGCGCTCCATCACTTCATCATAGTTCAGGACATCGCCTTTGATCGGTTCAGa                 >  1:1382234/1‑59 (MQ=255)

                                                                tGATCCATGCGCTCCATCACTTCATCATAGTTCAGGACATCGCCTTTGATCGGTTCAGACTTCGGACCAAc     >  1:30573/1‑71 (MQ=255)

                                                                   tCCATGCGCTCCATCACTTCATCATAGTTCAGGACATCGCCTTTGATCGGTTCAGACTTCGGACCAAc     <  1:5052332/68‑1 (MQ=255)

                                                                   tCCATGCGCTCCATCACTTCATCATAGTTCAGGACATCGCCTTTGATCGGTTCAGACTTCGGACCAAc     <  1:2257628/68‑1 (MQ=255)

                                                                   tCCATGCGCTCCATCACTTCATCATAGTTCAGGACATCGCCTTTGATCGGTTCAGACTTCGGACCAAc     <  1:1831273/68‑1 (MQ=255)

                                                                   tCCATGCGCTCCATCACTTCATCATAGTTCAGGACATCGCCTTTGATCGGTTCAGACTTCGGACCAACCTg  <  1:1473586/71‑1 (MQ=255)

                                                                    ccATGCGCTCCATCACTTCATCATAGTTCAGGACATCGCCTTTGATCGGTTCAGACTTc             >  1:5469228/1‑59 (MQ=255)

                                                                     cATGCGCTCCATCACTTCATCATAGTTCAGGACATCGCCTTTGATCGGTTCAGACTTc             <  1:669781/58‑1 (MQ=255)

GTCGTGCATGTAGTGGATGATGTTCAGTGCAGTGATGTACTGTTTAGCCAGCCAGTCCATGAAGTGATCCATGCGCTCCATCACTTCATCATAGTTCAGGACATCGCCTTTGATCGGTTCAGACTTCGGACCAACCTG  >  Exported/608745‑608882

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`