| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 609,165 | G→A | 10.5% | T363T (ACC→ACT) | pflB ← | pyruvate formate lyase I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 609,165 | 0 | G | A | 10.5% | 152.2 / 7.2 | 57 | T363T (ACC→ACT) | pflB | pyruvate formate lyase I |
| Reads supporting (aligned to +/- strand): ref base G (25/26); new base A (4/2); total (29/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.70e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
ATTTCTTGAAGTTCAGCGGCAGTTTTTCAGACCACAGAATGGTCATGTTCGGTTCCGGAGACGGACCCATGGTGTACAGGGTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATACCA > Exported/609095‑609234 | aTTTCTTGAAGTTCAGCGGCAGTTTTTCAGACCACAGAATGGTCATGTTCGGTTCCGGAGACGGACCCATg > 1:2855274/1‑71 (MQ=255)aTTTCTTGAAGTTCAGCGGCAGTTTTTCAGACCACAGAATGGTCATGTTCGGTTCCGGAGACGGACCCATg > 1:237818/1‑71 (MQ=255) cTTGAAGTTCAGCGGCAGTTTTTCAGACCACAGAATGGTCATGTTCGGTTCCGGAGACGGACCCATGgtg < 1:3231649/70‑1 (MQ=255) cTTGAAGTTCAGCGGCAGTTTTTCAGACCACAGAATGGTCATGTTCGGTTCCGGAGACGGACCCATGgtg < 1:4803976/70‑1 (MQ=255) gTTCAGCGGCAGTTTTTCAGACCACAGAATGGGCATGTTCGGTTCCGGAGACGGACCCATGGGGTACAggg < 1:630309/71‑1 (MQ=255) ttCAGCGGCAGTTTTTCAGACCACAGAATGGTCATGTTCGGTTCCGGAGACGGACCCATGGTGTACAGGgt < 1:1283183/71‑1 (MQ=255) cAGCGGCAGTTTTTCAGACCACAGAATGGTCATGTTCGGTTCCGGAGACGGACCCATGGTGTACAGGgtg < 1:3811779/70‑1 (MQ=255) cAGTTTTTCAGACCACAGAATGGTCATGTTCGGTTCCGGAGACGGACCCATGGTGTACAGGGTGTTCAgg > 1:2101799/1‑70 (MQ=255) tttttCAGACCACAGAATGGTCATGTTCGGTTCCGGAGACGGACCCATGGTGTACa < 1:5682322/56‑1 (MQ=255) ttttCAGACCACAGAATGGTCATGTTCGGTTCCGGAGACGGACCCATGGTGTAc > 1:5387739/1‑54 (MQ=255) tttCAGACCACAGAATGGTCATGTTCGGTTCCGGAGACGGACCCATGGTGTACa > 1:3332314/1‑54 (MQ=255) ttCCGACCAAAGAATGGTCAGGTTAGGTTCCGGTGCCGGCCCCATAGTGTGCAg < 1:1147016/54‑1 (MQ=255) ttCAGACCACAGAATGGTCATGTTCGGTTCCGGAGACGGACCCATGGTGTACAGGGTGTTCAGGAAACgg < 1:5219778/70‑1 (MQ=255) tCAGACCACAGAATGGTCATGTTCGGCTCCGGAGACGGACCCATGGTGTACAGGGTGTTCAGGAAACGGa < 1:3006246/70‑1 (MQ=255) ccACAGAATGGTCATGTTCGGTTCCGGAGACGGACCCATGGTGTACAGGGTGTTCAGGAAACGGAAGCTGt > 1:5574060/1‑71 (MQ=255) acaGAATGGTCATGTTCGGTTCCGGAGACGGACCCATGGTGTACAGGGTGTTCAGGAAACGGAAGCTGttt < 1:5498938/71‑1 (MQ=255) caGAATGGTCATGTTCGGTTCCGGAGACGGACCCATGGTGTACAGGGTGTTCAGGAAACGGAAGCTGtttt < 1:3007695/71‑1 (MQ=255) gAATGGTCATGTTCGGTTCCGGAGACGGACCCATGGTGTACAg < 1:5652737/43‑1 (MQ=255) gAATGGTCATGTTCGGTTCCGGAGACGGACCCATGGTGTACAGGGTGTTCAGGAAACGGAAGCTGttttt > 1:324917/1‑70 (MQ=255) aaTGGTCATGTTCGGTTCCGGAGACGGACCCATGGTGTACAGGGTGTTCAGGAAACGGAAGCTGtttt < 1:4267762/68‑1 (MQ=255) aaTGGTCATGTTCGGTTCCGGAGACGGACCCATGGTGTACAGGGTGTTCAGGAAACGGAAGCTGtttt < 1:333433/68‑1 (MQ=255) tGGTCATGTTCGGTTCCGGAGACGGACCCATGGTGTACa > 1:1275916/1‑39 (MQ=255) tCATGTTCGGTTCCGGAGACGGACCCATGGTGTACAGGgtg > 1:1903412/1‑41 (MQ=255) tCATGTTCGGTTCCGGAGACGGACCCATGGTGTACAGGGTGTTCAGGAAACggg > 1:4442518/1‑53 (MQ=255) tCATGTTCGGTTCCGGAGACGGACCCATGGTGTACAGGGTGTTCAGGAAACgg > 1:3008823/1‑53 (MQ=255) aTGTTCGGTTCCGGAGACGGACCCATGGTGTACAGGGTGTTCAGGAAACGGAAGCTGTTTTTGGTa > 1:3061706/1‑66 (MQ=255) aTGTTCGGTTCCGGAGACGGACCCATGGTGTACAGGGTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAg > 1:1055736/1‑71 (MQ=255) tGTTCGGTTCCGGAGACGGACCCATGGTGTACAGGGTGTTc < 1:4275667/41‑1 (MQ=255) tGTTCGGTTCCGGAGACGGACCCATGATGTACAGGGTGTTc < 1:4775169/41‑1 (MQ=255) gTTCGGTTCCGGAGACGGACCCATGGTGTACAGGGTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAggg > 1:1284140/1‑71 (MQ=255) ttCGGTTCCGGAGACGGACCCATGGTGTACAGGGTGTTCAGGAAACGGAAg < 1:3889965/51‑1 (MQ=255) ttCGGTTCCGGAGACGGACCCATGGTGTACAGGGTGTTCAGGAAACGGAAGCTGtt > 1:3038035/1‑56 (MQ=255) ttCGGTTCCGGAGACGGACCCATGGTGTACAGGGTGTTCAGGAAACGGAAGCTGTTTTTGGTaa > 1:260483/1‑64 (MQ=255) ttCGGTTCCGGAGACGGACCCATGGTGTACAGGGTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAggg > 1:675934/1‑70 (MQ=255) tCGGTTCCGGAGACGGACCCATGGTGTACAGGGTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTa > 1:251769/1‑71 (MQ=255) tCGGTTCCGGAGACGGACCCATGGTGTACAGGGTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTa > 1:359129/1‑71 (MQ=255) cGGTTCCGGAGACGGACCCATGGTGTACAGGGTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTAc > 1:5628799/1‑71 (MQ=255) ggTTCCGGAGACGGACCCATGGTGTACAGGGTGTTCAGGAAACGGa < 1:1371647/46‑1 (MQ=255) ggTTCCGGAGACGGACCCATGGTGTACAGGGTGTTCAGGAAACGGa < 1:663643/46‑1 (MQ=255) ggTTCCGGAGACGGACCCATGGTGTACAGGGTGTTCAGGAAACGGa < 1:3070475/46‑1 (MQ=255) ccGGAGACGGACCCATGGTGTACAGGGTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCg > 1:3917869/1‑71 (MQ=255) cGGAGACGGACCCATGGTGTACAGGGTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCg < 1:4329675/70‑1 (MQ=255) ggAGACGGACCCATGGTGTACAGGGTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTc < 1:5267283/71‑1 (MQ=255) ggAGACGGACCCATGGTGTACAGGGTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTc < 1:4430501/71‑1 (MQ=255) gaCGGACCCATGGTGTACAGGGTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAg < 1:1688737/71‑1 (MQ=255) ggccccATAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGccc > 1:88760/4‑71 (MQ=255) ggACCCATGGTGTACAGGGTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAgg > 1:3280802/1‑54 (MQ=255) ggACCCATGGTGTACAGGGTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGcc < 1:4683451/71‑1 (MQ=255) ggACCCATGGTGTACAGGGTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGcc > 1:4277363/1‑71 (MQ=255) ggACCCATGGTGTACAGGGTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGcc < 1:831753/71‑1 (MQ=255) gccccATAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGccc > 1:1969608/3‑70 (MQ=255) gccccATAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGccc > 1:5585024/3‑70 (MQ=255) cccATAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCAt > 1:2876034/1‑71 (MQ=255) ccATGGTGTACAGGGTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGa > 1:1727957/1‑63 (MQ=255) ccATGGTGTACAGGGTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGa > 1:3538263/1‑63 (MQ=255) cATGGTGTACAGGGTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATac < 1:1498445/71‑1 (MQ=255) cATAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCAtcc < 1:28585/71‑3 (MQ=255) aTGGTGTACAGGGTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATac > 1:3725049/1‑70 (MQ=255) tGGTGTACAGGGTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATacca > 1:1355781/1‑71 (MQ=255) | ATTTCTTGAAGTTCAGCGGCAGTTTTTCAGACCACAGAATGGTCATGTTCGGTTCCGGAGACGGACCCATGGTGTACAGGGTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATACCA > Exported/609095‑609234 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |