Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	609,225	G→C	21.8%	G343G (GGC→GGG)	pflB ←	pyruvate formate lyase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	609,225	0	G	C	21.8%	181.2 / 42.9	87	G343G (GGC→GGG)	pflB	pyruvate formate lyase I
Reads supporting (aligned to +/- strand):  ref base G (35/33);  new base C (11/8);  total (46/41)
Fisher's exact test for biased strand distribution p-value = 7.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GGACCCATGGTGTACAGGGTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAG  >  Exported/609157‑609292                                                                     |                                                                    ggccccATAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGccc                                                                   >  1:88760/4‑71 (MQ=255) ggACCCATGGTGTACAGGGTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGcc                                                                   >  1:4277363/1‑71 (MQ=255) ggACCCATGGTGTACAGGGTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGcc                                                                   <  1:4683451/71‑1 (MQ=255) ggACCCATGGTGTACAGGGTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGcc                                                                   <  1:831753/71‑1 (MQ=255)  gccccATAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGccc                                                                   >  1:5585024/3‑70 (MQ=255)  gccccATAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGccc                                                                   >  1:1969608/3‑70 (MQ=255)    cccATAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCAt                                                                >  1:2876034/1‑71 (MQ=255)      cATGGTGTACAGGGTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATac                                                              <  1:1498445/71‑1 (MQ=255)      cATAGTGTGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCAtcc                                                              <  1:28585/71‑3 (MQ=255)       aTGGTGTACAGGGTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATac                                                              >  1:3725049/1‑70 (MQ=255)        tGGTGTACAGGGTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATacca                                                            >  1:1355781/1‑71 (MQ=255)          gtgtGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATcccgcc                                                          >  1:3131886/1‑71 (MQ=255)          gtgtGCAGGGTGTGCAAATAGCGGAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATcccgc                                                           >  1:4181715/1‑68 (MQ=255)          gtgtACAGGGTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATaccac                                                           >  1:1664215/1‑70 (MQ=255)             tACAGGGTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGAt                                                       <  1:2213120/71‑1 (MQ=255)               cAGGGTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGa                                                        >  1:1558318/1‑68 (MQ=255)               cAGGGTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATa                                                      <  1:4373535/70‑1 (MQ=255)                 gggTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATAACACCGATAGAt                                                   <  1:4219930/71‑1 (MQ=255)                      ttCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGcc                                                                   <  1:2075826/50‑1 (MQ=255)                      ttCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGcc                                                                   <  1:1232033/50‑1 (MQ=255)                      ttCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGt                                              >  1:146097/1‑71 (MQ=255)                        cAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATac                                                              >  1:1681998/1‑53 (MQ=255)                         aGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTg                                            <  1:1815458/70‑1 (MQ=255)                         aGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGc                                           <  1:3223435/71‑1 (MQ=255)                             aaCGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCAt                                                                >  1:180679/1‑46 (MQ=255)                             aaCGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAg                                       <  1:5120824/71‑1 (MQ=255)                              aCGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGa                                      <  1:1618778/71‑1 (MQ=255)                               cGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCg                                                         >  1:254371/1‑51 (MQ=255)                               cGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGa                                                        >  1:1655566/1‑52 (MQ=255)                               cGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGa                                                        >  1:3229867/1‑52 (MQ=255)                               cGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGcc                                          >  1:2858751/1‑66 (MQ=255)                               cGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGAt                                     <  1:1468218/71‑1 (MQ=255)                                ggAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGAt                                                       >  1:2928662/1‑52 (MQ=16)                                ggAAGGAGTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGAt                                                       >  1:3848782/1‑52 (MQ=16)                                ggAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCAt                                                                >  1:3627170/1‑43 (MQ=255)                                ggAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATa                                                               >  1:2470007/1‑44 (MQ=255)                                ggAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGcc                                          >  1:4980736/1‑65 (MQ=255)                                   aGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCgg                                  <  1:2476638/70‑1 (MQ=255)                                    ggagTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCGCCCAGATTGGGt                                <  1:2964678/68‑1 (MQ=255)                                       gTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGAtca                                                     >  1:5579429/1‑45 (MQ=16)                                       gTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGAtca                                                     >  1:417144/1‑45 (MQ=16)                                       gTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGAtca                                                     >  1:1750265/1‑45 (MQ=16)                                       gTTTTTGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGAtca                                                     >  1:2007762/1‑45 (MQ=16)                                       gTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCgg                                               >  1:4654558/1‑53 (MQ=255)                                        tttttGGTCACCAGCGTACGACCGTCCAGCCCCATcccgc                                                           <  1:2577542/40‑3 (MQ=12)                                        tttttGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGAtc                                                      <  1:3875433/45‑2 (MQ=12)                                        tttttGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCGCCCAGATTggg                                 >  1:4588029/1‑66 (MQ=255)                                        tttttGGTAACCAGGGTACGACCGTCGAGGCCCATa                                                               <  1:1174576/36‑1 (MQ=255)                                        tttttGGTAACCAGGGTACGACCGTCGAGGCCCATa                                                               <  1:1735616/36‑1 (MQ=255)                                        tttttGGTAACCAGGGTACGACCGTCGAGGCCCATa                                                               <  1:3161205/36‑1 (MQ=255)                                        tttttGGTAACCAGGGTACGACCGTCGAGGCCCATa                                                               <  1:1202690/36‑1 (MQ=255)                                        tttttGGTAACCAGGGTACGACCGTCGAGGCCCATa                                                               <  1:3431981/36‑1 (MQ=255)                                        tttttGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGc                             <  1:151642/70‑1 (MQ=255)                                         ttttGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGc                             >  1:2138134/1‑69 (MQ=255)                                         ttttGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGc                             >  1:1515688/1‑69 (MQ=255)                                          tttGGTAACCAGGGTACGACCGTCGAGGCCCATacca                                                            >  1:1993855/1‑37 (MQ=255)                                          tttGGTAACCAGGGTACGACCGTCGAGGCCCATacc                                                             >  1:266622/1‑36 (MQ=255)                                          tttGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCg                                   >  1:2000489/1‑62 (MQ=255)                                          tttGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGcc                            >  1:2712682/1‑69 (MQ=255)                                          tttGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGcc                            >  1:2474570/1‑69 (MQ=255)                                           ttGGTCACCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCGCCCAGATTGGGTCGccgg                          <  1:1269378/70‑3 (MQ=255)                                             ggTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCagag                        >  1:1969865/1‑70 (MQ=255)                                              gtcaCCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCGCCCAGATTGGGTCGCCGGAGaa                      <  1:4104932/68‑1 (MQ=255)                                              gTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAgaga                       <  1:2512797/70‑1 (MQ=255)                                                 aCCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAg                   >  1:845085/1‑71 (MQ=255)                                                 aCCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCGCCCAGATTGGGTCGcc                            <  1:765651/62‑1 (MQ=255)                                                 aCCAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCGCCCAGATTGGGTCGCCGGAGAACAg                   <  1:3508966/71‑1 (MQ=255)                                                   cAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGtt                 >  1:4221212/1‑71 (MQ=255)                                                   cAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGt                  >  1:2647042/1‑70 (MQ=255)                                                   cAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGt                  >  1:3660695/1‑70 (MQ=255)                                                   cAGCGTACGACCGTCCAGCCCCATCCCGCCGATCACTTCCGTCGCCCAGATTGGGTCGccgg                          >  1:2796722/1‑60 (MQ=255)                                                      ggTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCAt              <  1:1293650/71‑1 (MQ=255)                                                        tACGACCGTCGAGGCCCATACCACCGATAGATTCgg                                               >  1:4678409/1‑36 (MQ=255)                                                        tACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCg            <  1:2079290/71‑1 (MQ=255)                                                        tACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCg            <  1:3889893/71‑1 (MQ=255)                                                          cGACCGTCGAGGCCCATACCACCGATAGATTCGGTTg                                            <  1:3613668/37‑1 (MQ=255)                                                          cGACCGTCGAGGCCCATACCACCGATAGATTCGGTTg                                            <  1:3607041/37‑1 (MQ=255)                                                            aCCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTAtt        >  1:1698968/1‑71 (MQ=255)                                                            aCCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTAt         >  1:624818/1‑70 (MQ=255)                                                             ccGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTc       >  1:4124624/1‑71 (MQ=255)                                                             ccGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTc       >  1:572776/1‑71 (MQ=255)                                                               gTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCgg                                  <  1:2518763/42‑1 (MQ=255)                                                               gTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCg     <  1:1691527/71‑1 (MQ=255)                                                               gTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCg     <  1:850734/71‑1 (MQ=255)                                                                tCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACa                    <  1:4986670/55‑1 (MQ=255)                                                                tCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACa                    <  1:4247526/55‑1 (MQ=255)                                                                tCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACa                    <  1:3899602/55‑1 (MQ=255)                                                                tCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACa                    <  1:3307660/55‑1 (MQ=255)                                                                tCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCg     >  1:4205076/1‑70 (MQ=255)                                                                   aGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAg  >  1:4514107/1‑70 (MQ=255)                                                                     |                                                                    GGACCCATGGTGTACAGGGTGTTCAGGAAACGGAAGCTGTTTTTGGTAACCAGGGTACGACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAG  >  Exported/609157‑609292

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑