Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	609,283	T→A	6.2%	Y324F (TAC→TTC) ‡	pflB ←	pyruvate formate lyase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	609,283	0	T	A	6.2%	278.1 / 7.9	97	Y324F (TAC→TTC) ‡	pflB	pyruvate formate lyase I
Reads supporting (aligned to +/- strand):  ref base T (61/30);  new base A (4/2);  total (65/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.36e-01

ACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTT  >  Exported/609216‑609352                                                                    |                                                                      aCCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTAtt                                                                    >  1:1698968/1‑71 (MQ=255) aCCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTAt                                                                     >  1:624818/1‑70 (MQ=255)  ccGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTc                                                                   >  1:4124624/1‑71 (MQ=255)  ccGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTc                                                                   >  1:572776/1‑71 (MQ=255)    gTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCg                                                                 <  1:850734/71‑1 (MQ=255)    gTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCg                                                                 <  1:1691527/71‑1 (MQ=255)     tCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCg                                                                 >  1:4205076/1‑70 (MQ=255)        aGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAg                                                              >  1:4514107/1‑70 (MQ=255)            ccATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTGCGGAGTACg                                                          >  1:3484677/1‑70 (MQ=255)              aTACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAg                                                       >  1:4629968/1‑71 (MQ=255)               tACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAgg                                                      <  1:3820930/71‑1 (MQ=255)               tACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAgg                                                      <  1:1712763/71‑1 (MQ=255)                 ccaccGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGa                                                     <  1:3361070/70‑1 (MQ=255)                 ccaccGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGa                                                     <  1:821195/70‑1 (MQ=255)                   accGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCg                                                                 >  1:378396/1‑56 (MQ=255)                    ccGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTc                                                                   >  1:5616054/1‑53 (MQ=255)                     cGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCg                                                 <  1:1657633/70‑1 (MQ=255)                       aTAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAAc                                              >  1:4952182/1‑71 (MQ=255)                        tAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTc                                                                   <  1:4114301/49‑1 (MQ=255)                        tAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGa                                                <  1:2988351/68‑1 (MQ=255)                         aGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGc                                                  >  1:1460356/1‑65 (MQ=255)                         aGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAAcc                                             >  1:5282517/1‑70 (MQ=255)                         aGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAAc                                              >  1:4756260/1‑69 (MQ=255)                         aGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCa                                            >  1:399609/1‑71 (MQ=255)                          gATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCa                                            <  1:4702818/70‑1 (MQ=255)                          acttCCGTCGCCCAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCa                                            >  1:5649674/3‑70 (MQ=255)                              cGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACg                                        >  1:4007674/1‑70 (MQ=255)                                   gCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGtt                                   >  1:1803539/1‑70 (MQ=255)                                    cccAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTc                                 <  1:5186223/71‑1 (MQ=255)                                    cccAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTc                                 <  1:3545860/71‑1 (MQ=255)                                     ccAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCa                                >  1:3512965/1‑71 (MQ=255)                                      cAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCAt                               <  1:2266684/71‑1 (MQ=255)                                      cAGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCAt                               <  1:3130415/71‑1 (MQ=255)                                      cAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCg                                                 <  1:42153/53‑1 (MQ=255)                                      cAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCg                                                 <  1:4897229/53‑1 (MQ=255)                                      cAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCg                                                 <  1:3613448/53‑1 (MQ=255)                                       aGATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCAt                               >  1:3455217/1‑70 (MQ=255)                                       aGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCAt                               >  1:123839/1‑70 (MQ=255)                                       aGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCAt                               >  1:4891136/1‑70 (MQ=255)                                       aGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCAt                               >  1:3794498/1‑70 (MQ=255)                                       aGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCAt                               >  1:3589419/1‑70 (MQ=255)                                       aGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCAt                               >  1:2193704/1‑70 (MQ=255)                                       aGATCGGGTCGCCAGAGAACAGTTCATCGTATTACGGAGTACGCAGGAAGCGAACCATACGCAGTTTCAt                               >  1:4897799/1‑70 (MQ=255)                                        gATTGGGTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGa                             >  1:3407185/1‑71 (MQ=255)                                        gATCGGGTCGCCAGAGAACAGTTCATCGTATTTCGGAGTACGCAGGAAGCGa                                                >  1:190192/1‑52 (MQ=255)                                        gATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGc                                                         <  1:2008814/43‑1 (MQ=255)                                        gATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGaa                                               >  1:5165173/1‑53 (MQ=255)                                        gATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGaa                                               >  1:2798119/1‑53 (MQ=255)                                        gATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGaa                                               >  1:4145257/1‑53 (MQ=255)                                         aTCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGa                                                               >  1:5447180/1‑36 (MQ=255)                                         aTCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGa                                                               >  1:440374/1‑36 (MQ=255)                                         aTCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGAc                            <  1:3607582/71‑1 (MQ=255)                                          ttgggTCGCCGGAGAACAGCGAATCAAATTCCGGTGTACGCAGGAAGCGTACCATACGGATCTTCATGa                             >  1:1024587/3‑69 (MQ=255)                                          tCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCa                                                        >  1:352507/1‑42 (MQ=255)                                          tCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGa                                                >  1:1816350/1‑50 (MQ=255)                                          tCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATa                                          >  1:3601350/1‑56 (MQ=255)                                          tCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATa                                          >  1:23699/1‑56 (MQ=255)                                          tCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGAcc                           <  1:3888221/71‑1 (MQ=255)                                           cGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAAcc                                             >  1:5177806/1‑52 (MQ=255)                                           cGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAAcc                                             >  1:3362506/1‑52 (MQ=255)                                           cGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAAcc                                             >  1:5637492/1‑52 (MQ=255)                                           cGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAAcc                                             >  1:2447193/1‑52 (MQ=255)                                           cGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGttt                                  >  1:722803/1‑63 (MQ=255)                                           cGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCa                                >  1:5626028/1‑65 (MQ=255)                                           cGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGAcc                           <  1:2468755/70‑1 (MQ=255)                                           cGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGAcc                           <  1:5527717/70‑1 (MQ=255)                                           cGGGTCGCCAGAGAACAGTTCATCGTATTACGGAGTACGCAGGAAGCg                                                 >  1:5453611/1‑48 (MQ=255)                                             ggTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCa                                            <  1:2814762/51‑1 (MQ=255)                                                cGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAggtg                      >  1:4023666/1‑70 (MQ=255)                                                 gCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCg                                                 >  1:3567643/1‑42 (MQ=255)                                                 gCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGAc                            >  1:4516847/1‑63 (MQ=255)                                                 gCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAggtggt                    >  1:3271539/1‑71 (MQ=255)                                                 gCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAggtggt                    >  1:5303577/1‑71 (MQ=255)                                                   cAGAGAACAGTTCATCGTATTCCTGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCa                  <  1:5031927/71‑1 (MQ=255)                                                   cAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGt                                    <  1:228596/53‑1 (MQ=255)                                                   cAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCa                  <  1:278814/71‑1 (MQ=255)                                                    agagAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAg                         >  1:1972603/1‑63 (MQ=255)                                                     gagaACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATa                                          <  1:4348556/45‑1 (MQ=255)                                                     gagaACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCACCTGGTCat                 >  1:2931471/1‑69 (MQ=255)                                                     gagaACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCACCTGGTCat                 >  1:878281/1‑69 (MQ=255)                                                       gaACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCa              <  1:1885619/71‑1 (MQ=255)                                                         aCAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGCTTCATGACCAGGTGGTCAACCAtt            >  1:1719359/1‑71 (MQ=255)                                                         aCAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGAAGTTTCATGACCAGGTGGTCAACCAtt            >  1:4089161/1‑71 (MQ=255)                                                          cAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCAtt            >  1:2999037/1‑70 (MQ=255)                                                             ttCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGttt                                  <  1:4923374/45‑1 (MQ=255)                                                             ttCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGttt                                  <  1:2220146/45‑1 (MQ=255)                                                             ttCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGttt                                  <  1:4537443/45‑1 (MQ=255)                                                             ttCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGttt                                  <  1:64744/45‑1 (MQ=255)                                                              tCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAg                                     >  1:4645613/1‑41 (MQ=255)                                                              tCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAg                                     >  1:2543439/1‑41 (MQ=255)                                                               cATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCa                                >  1:2829690/1‑45 (MQ=255)                                                               cATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCa                                >  1:5633186/1‑45 (MQ=255)                                                               cATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCGACCATTTCCtac      >  1:3684893/1‑69 (MQ=255)                                                               cATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTgc      >  1:3693472/1‑71 (MQ=255)                                                               cATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTgc      >  1:1981534/1‑71 (MQ=255)                                                               cATCGTATTCCGGAGTAAGCAGGAAGCGAACCATACGCAGTTTCa                                >  1:5174622/1‑45 (MQ=255)                                                                   gTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGctt  >  1:4107251/1‑71 (MQ=255)                                                                    |                                                                      ACCGTCGAGGCCCATACCACCGATAGATTCGGTTGCCCAGATCGGGTCGCCAGAGAACAGTTCATCGTATTCCGGAGTACGCAGGAAGCGAACCATACGCAGTTTCATGACCAGGTGGTCAACCATTTCCTGCGCTT  >  Exported/609216‑609352

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑