| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 609,699 | A→T | 12.6% | V185V (GTT→GTA) | pflB ← | pyruvate formate lyase I |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 609,699 | 0 | A | T | 12.6% | 250.1 / 25.2 | 95 | V185V (GTT→GTA) | pflB | pyruvate formate lyase I |
| Reads supporting (aligned to +/- strand): ref base A (49/34); new base T (6/6); total (55/40) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.56e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.81e-01 | |||||||||||
Only 100 of 103 total aligned reads displayed.
CAGATCAGCCTGCAGAGAAGTGAACTGTGCCAGTTTGTCTTTCATCAGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATT > Exported/609630‑609769 | cAGATCAGCCTGCAGAGAAGTGAACTGTGCCAGTTTGTCTTTCATCAGGTAGTCGATACCGTACAGCGCaa < 1:2895668/71‑1 (MQ=255)cAGATCAGCCTGCAGAGAAGTGAACTGTGCCAGTTTGTCTTTCATCAGGTAGTCGATACCGTACAGCGCa < 1:371256/70‑1 (MQ=255)cAGATCAGCCTGCAGAGAAGTGAACTGTGCCAGTTTGTCTTTCATCAGGTAGTCGATACCGTACAGCGCa < 1:3961878/70‑1 (MQ=255) aGCCTGCAGAGAAGTGAACTGTGCCAGTTTGTCTTTCATCAGGTAGTCGATACCGTACAGCGCAACGCGAc > 1:686992/1‑71 (MQ=255) aGCCTGCAGAGAAGTGAACTGTGCCAGTTTGTCTTTCATCAGGTAGTCGATACCGTACAGCGCAACGCGAc > 1:3186792/1‑71 (MQ=255) cTGCAGAGAAGTGAACTGTGCCAGTTTGTCTTTCATCAGGTAGTCGATACCGTACAGCGCAACGCGACGGt < 1:927197/71‑1 (MQ=255) gaAGTGAACTTTTCCAGTTTGTCTTTCATCAGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCAcc < 1:161517/71‑1 (MQ=255) aaGTGAACTGTGCCAGTTTGTCTTTCATCAGGTAGTCGATACCGTACAGCGCAACGCGACgg < 1:757716/62‑1 (MQ=255) aGTGAACTGTGCCAGTTTGTCTTTCATCAGGTAGTCGATACCGTACAGCGCAACGCGACgg < 1:1958647/61‑1 (MQ=255) aGTGAACTGTGCCAGTTTGTCTTTCATCAGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCg < 1:5489663/70‑1 (MQ=255) gTGAACTGTGCCAGTTTGTCTTTCATCAGGTAGTCGATACCGTACAGCGCaa < 1:4461444/52‑1 (MQ=255) gTGAACTGTGCCAGTTTGTCTTTCATCAGGTAGTCGATACCGTACAGCGCaa < 1:5597802/52‑1 (MQ=255) gAACTGTGCCAGTTTGTCTTTCATCAGGTAGTCGATACCGTACAGCGCAACGCGACagtagtaa > 1:4271934/1‑62 (MQ=255) gAACTGTGCCAGTTTGTCTTTCATCAGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCga > 1:1597522/1‑68 (MQ=255) gAACTCTGCCAGTTTGTCTTTCATCAGGTAGTCGATACCGTACAGCGCAACGCGACAGTAGTCa > 1:4278588/1‑64 (MQ=255) aaCTGTGCCAGTTTGTCTTTCATCAGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCgatga > 1:5592300/1‑70 (MQ=255) aCTGTGCCAGTTTGTCTTTCATCAGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCgatga < 1:4533258/69‑1 (MQ=255) cTGTGCCAGTTTGTCTTTCATCAGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCgatga < 1:1501583/68‑1 (MQ=255) tgtgCCAGTTTGTCTTTCATCAGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATacg > 1:227242/1‑71 (MQ=255) tgCCAGTTTGTCTTTCATCAGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCgatga > 1:549199/1‑65 (MQ=255) tgCCAGTTTGTCTTTCATCAGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCgatga > 1:5575671/1‑65 (MQ=255) tgCCAGTTTGTCTTTCATCAGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATacggc > 1:58150/1‑71 (MQ=255) tgCCAGTTTGTCTTTCATCAGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATacggc > 1:274207/1‑71 (MQ=255) cAGTTTGTCTTTCATCAGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCAcc < 1:1285523/57‑1 (MQ=255) cAGTTTGTCTTTCATCAGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATacggccac > 1:4086692/1‑71 (MQ=255) cAGTTTGTCTTTCATCAGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATacggccac < 1:3335765/71‑1 (MQ=255) ttGTCTTTCATCAGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCGATTATacggccacggc < 1:939193/70‑1 (MQ=255) tGTCTTTCATCAGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCGCCgatgat > 1:1554669/1‑58 (MQ=255) tGTCTTTCATCAGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCgatgat > 1:5210927/1‑58 (MQ=255) tGTCTTTCATCAGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCgatgat > 1:1510659/1‑58 (MQ=255) tGTCTTTCATCAGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATacggccac > 1:3453361/1‑66 (MQ=255) gTCTTTCATCAGGTAGTCGATACCGTACAGCGCAACGCGACGGTa > 1:2004540/1‑45 (MQ=255) gTCTTTCATCAGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATAcggccacggcca > 1:4368058/1‑70 (MQ=255) gTCTTTCATCAGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATAcggccacggcca < 1:4981263/70‑1 (MQ=255) gTCTTTCATCAGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATAcggccacggcca < 1:4987662/70‑1 (MQ=255) cTTTCATCAGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCgatg > 1:4441436/1‑53 (MQ=255) cTTTCATCAGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCgatg > 1:1216460/1‑53 (MQ=255) cTTTCATCAGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCgatg > 1:1497996/1‑53 (MQ=255) cTTTCATCAGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCgatg > 1:1698368/1‑53 (MQ=255) ttCATCAGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCata > 1:572454/1‑68 (MQ=255) tcatcaGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGc < 1:2795615/70‑1 (MQ=255) tcatcaGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCa > 1:5208177/1‑71 (MQ=255) atcaGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCAt < 1:739018/70‑1 (MQ=255) tcaGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCt < 1:2921582/71‑1 (MQ=255) tcaGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCt < 1:5165410/71‑1 (MQ=255) gTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATAcggccacggcca < 1:3335217/58‑1 (MQ=255) gTCGATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGAc < 1:1626945/71‑1 (MQ=255) tCGATACCGTACAGCGCACCGCGACGGTAGTCAcc < 1:2471051/35‑1 (MQ=255) tCGATACCGTACAGCGCAACGCGACGGTAGTCAcc < 1:2487276/35‑1 (MQ=255) tCGATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGAc < 1:5438625/70‑1 (MQ=255) tgATGCCATACAGCGCTACGCGGCGATAGTCACCGATAATGCGCCCACGGCCATAGCCATCTGGTAAACCg > 1:4832129/2‑71 (MQ=255) cGATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCg > 1:3647090/1‑71 (MQ=255) gATACCTTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCgg > 1:2364034/1‑71 (MQ=255) gATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATAcggccacggcca < 1:2112933/52‑1 (MQ=255) gATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATAcggccacggcca < 1:4626382/52‑1 (MQ=255) gATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCgg > 1:4304771/1‑71 (MQ=255) tACCGTACAGCGCAACGCGACGGTAGTCACCGATGATacggccacggc > 1:3762709/1‑48 (MQ=255) tACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCAt > 1:5246443/1‑57 (MQ=255) tACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCAt > 1:867204/1‑57 (MQ=255) tACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCAt > 1:2385760/1‑57 (MQ=255) tACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCAt > 1:3107127/1‑57 (MQ=255) tACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCAt > 1:5135949/1‑57 (MQ=255) tACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGt > 1:652340/1‑70 (MQ=255) tACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGt > 1:1882342/1‑70 (MQ=255) tACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGACACGGCCATATGCAt > 1:5125436/1‑57 (MQ=255) ccatACAGCGCTACGCGGCGATAGTCACCGATAATGCGCCCACGGCCATAGCCATGTGGTAAACCGGTCAg < 1:3584754/68‑1 (MQ=255) ccatACAGCGCTACGCGGCGATAGTCACCGATAATGCGCCCACGGCCATAGCCATCTGGTAAACCGGTCAg < 1:1652425/68‑1 (MQ=255) ccGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCata < 1:1825870/50‑1 (MQ=255) ccGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCata < 1:3301076/50‑1 (MQ=255) ccGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAg < 1:4061678/71‑1 (MQ=255) ccGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAg < 1:819147/71‑1 (MQ=255) ccGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAg < 1:4882749/71‑1 (MQ=255) gTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGaa > 1:1245798/1‑71 (MQ=255) tACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCa < 1:4261029/51‑1 (MQ=255) tACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGaa > 1:1790933/1‑70 (MQ=255) aCAGCGCTACGCGGCGATAGTCACCGATAATGCGCCCACGGCCATAGCCATCTGGTAAACCGGTCAGCacg > 1:2452247/1‑70 (MQ=255) aCAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCa < 1:3353916/50‑1 (MQ=255) cAGCGCTACGCGGCGATAGTCACCGATAATGCGCCCACGGCCATAGCCATCTggt > 1:1897407/1‑54 (MQ=16) cAGCGCTACGCGGCGATAGTCACCGATAATGCGCCCACGGCCATAGCCATCTggt > 1:2969872/1‑54 (MQ=16) cAGCGCTACGCGGCGATAGTCACCGATAATGCGCCCACGGCCATAGCCATCTggt > 1:2963213/1‑54 (MQ=16) cAGCGCTACGCGGCGATAGTCACCGATAATGCGCCCACGGCCATAGCCATCTggt > 1:2959397/1‑54 (MQ=16) cAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCAt > 1:2296876/1‑50 (MQ=255) cAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATc > 1:2302745/1‑51 (MQ=255) cAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATc > 1:3114443/1‑51 (MQ=255) cAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAacac > 1:4111320/1‑71 (MQ=255) cAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAacac < 1:2323848/71‑1 (MQ=255) cAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAacac > 1:2925148/1‑71 (MQ=255) cAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAacac > 1:3290189/1‑71 (MQ=255) gcgcTACGCGGCGATAGTCACCGATAATGCGCCCACGGCCATAGCCATCTggta < 1:111725/54‑3 (MQ=12) cgcTACGCGGCGATAGTCACCGATAATGCGCCCACGGCCATAGCCATCTggta < 1:1037542/53‑3 (MQ=12) cgcTACGCGGCGATAGTCACCGATAATGCGCCCACGGCCATAGCCATCTggta < 1:4648563/53‑3 (MQ=12) cgcTACGCGGCGATAGTCACCGATAATGCGCCCACGGCCATAGCCATCTggta < 1:1468219/53‑3 (MQ=12) cgcAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAacac > 1:1550717/1‑68 (MQ=255) cgcAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAacac > 1:5112888/1‑68 (MQ=255) gcAACGCGACGGTAGTCACCGATGATAcggccacggcca < 1:2937721/39‑1 (MQ=255) gcAACGCGACGGTAGTCACCGATGATAcggccacggcca < 1:4342510/39‑1 (MQ=255) gcAACGCGACGGTAGTCACCGATGATAcggccacggcca < 1:4708354/39‑1 (MQ=255) aaCGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGAtt < 1:2322149/71‑1 (MQ=255) aaCGCGACGGTAGTCACCGATGATACGGCCAAGGCCATATGCATCTGGAAGAc > 1:3947898/1‑53 (MQ=39) aaCGCGACGGTAGTCACAGATGATACGGCCAAGGCCATATGCATCTGGCAGAc > 1:1878734/1‑53 (MQ=255) | CAGATCAGCCTGCAGAGAAGTGAACTGTGCCAGTTTGTCTTTCATCAGGTAGTCGATACCGTACAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATT > Exported/609630‑609769 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |