BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	609,762	A→G	33.3%	G164G (GGT→GGC)	pflB ←	pyruvate formate lyase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	609,762	0	A	G	33.3%	130.8 / 95.7	114	G164G (GGT→GGC)	pflB	pyruvate formate lyase I
Reads supporting (aligned to +/- strand): ref base A (50/26); new base G (17/21); total (67/47)
Fisher's exact test for biased strand distribution p-value = 4.33e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.24e-01

Only 100 of 120 total aligned reads displayed.

CAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTA  >  Exported/609693‑609828

cAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAacac                                                                   >  1:2925148/1‑71 (MQ=255)

cAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAacac                                                                   <  1:2323848/71‑1 (MQ=255)

cAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAacac                                                                   >  1:4111320/1‑71 (MQ=255)

   cgcAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAacac                                                                   >  1:5112888/1‑68 (MQ=255)

   cgcAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAacac                                                                   >  1:1550717/1‑68 (MQ=255)

    gctaCGCGGCGATAGTCACCGATAATGCGCCCACGGCCATAGCCATCTGGTAAACCGGTCAGCACGCCAGa                                                               <  1:3725503/68‑1 (MQ=255)

      aaCGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGAtt                                                             <  1:2322149/71‑1 (MQ=255)

       aCGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGAtt                                                             >  1:343396/1‑70 (MQ=255)

        cgcgGCGATAGTCACCGATAATGCGCCCACGGCCATAGCCATCTGGTAAACGGGTCAGCACGCCAGAtt                                                             <  1:2976377/69‑1 (MQ=255)

        cgcgGCGATAGTCACCGATAATGCGCCCACGGCCATAGCCATCTGGTAAACCGGTCAGCACGCCAGATTTa                                                           >  1:1043154/1‑71 (MQ=255)

        cgcgACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTa                                                           >  1:1977826/1‑71 (MQ=255)

        cgcgACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTa                                                           <  1:3262171/71‑1 (MQ=255)

        cgcgACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTa                                                           <  1:4904901/71‑1 (MQ=255)

            aCGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGc                                                       <  1:1586519/71‑1 (MQ=255)

            aCGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGc                                                       <  1:1593060/71‑1 (MQ=255)

                tAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACg                                                   >  1:3230450/1‑71 (MQ=255)

                  gTCACCGATAATGCGCCCACGGCCATAGCCATCTGGTAAACCGGTCAGCACGCCAGAttt                                                            <  1:267034/60‑1 (MQ=255)

                  gTCACCGATAATGCGCCCACGGCCATAGCCATCTGGTAAACCGGTCAGCACGCCAGAttt                                                            <  1:1618357/60‑1 (MQ=255)

                   tCACCGATAATGCGCCCACGGCCATAGCCATCTGGTAAACCGGTCAGCACGCCa                                                                 <  1:5566384/54‑1 (MQ=255)

                   tCACCGATAATGCGCCCACGGCCATAGCCATCTGGTAAACCGGTCAGCACGCCa                                                                 <  1:3137773/54‑1 (MQ=255)

                   tCACCGATAATGCGCCCACGGCCATAGCCATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAg                                                >  1:5050522/1‑71 (MQ=255)

                     aCCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACg                                                         >  1:5302453/1‑60 (MQ=255)

                     aCCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACg                                                         >  1:4480444/1‑60 (MQ=255)

                     aCCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGa                                              >  1:1910296/1‑71 (MQ=255)

                     aCCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGa                                              >  1:3452840/1‑71 (MQ=255)

                     aCCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGa                                              >  1:2137121/1‑71 (MQ=255)

                     aCCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGa                                              >  1:5385352/1‑71 (MQ=255)

                     aCCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGa                                              >  1:5506485/1‑71 (MQ=255)

                     aCCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGa                                              >  1:830147/1‑71 (MQ=255)

                     aCCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGa                                              >  1:3952179/1‑71 (MQ=255)

                       cGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATg                                            <  1:269486/71‑1 (MQ=255)

                        gatgatACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTa                                                           >  1:4793356/1‑55 (MQ=255)

                        gatgatACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTa                                                           >  1:2434345/1‑55 (MQ=255)

                        gatgatACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACg                                                         >  1:3314651/1‑57 (MQ=255)

                        gatgatACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACg                                                         >  1:2412064/1‑57 (MQ=255)

                        gataatGCGCCCACGGCCATAGCCATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCAtat                                           >  1:840490/1‑69 (MQ=255)

                         atgatACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTc                                          >  1:3351202/1‑71 (MQ=255)

                            atACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCg                                        <  1:1230781/70‑1 (MQ=255)

                            atACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCg                                        >  1:5603563/1‑70 (MQ=255)

                               cgcccacggccaTAGCCATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCAt                                             >  1:2696991/4‑62 (MQ=255)

                               cgcccacggccaTAGCCATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCAt                                             >  1:291098/4‑62 (MQ=255)

                                  ccacggccaTATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGTCAACGCAGGATGTCCGGAGTGTa                                 <  1:4426013/71‑1 (MQ=255)

                                  ccacggccaTATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTa                                 <  1:119365/71‑1 (MQ=255)

                                  ccacggccaTAGCCATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTa                                 <  1:4890959/71‑1 (MQ=255)

                                   cacggccaTATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTa                                 <  1:472761/70‑1 (MQ=255)

                                   cacggccaTAGCCATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTaa                                <  1:5234997/71‑1 (MQ=255)

                                    acggccaTAGCCATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTaaa                               >  1:1256356/1‑71 (MQ=255)

                                    acggccaTAGCCATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTa                                 <  1:5222851/69‑1 (MQ=255)

                                    acggccaTAGCCATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTa                                 <  1:826443/69‑1 (MQ=255)

                                     cggccaTATGCATCTGGCAGACCTTTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTaaa                               >  1:2340349/1‑70 (MQ=255)

                                     cggccaTATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTaaa                               >  1:1699264/1‑70 (MQ=255)

                                     cggccaTATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAAc                              >  1:2896784/1‑71 (MQ=255)

                                      ggccaTATGCATCTGGCAGACCGGTCAGAACACCAGAt                                                              >  1:391485/1‑38 (MQ=255)

                                      ggccaTATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGc                                                  >  1:709788/1‑50 (MQ=255)

                                      ggccaTAGCCATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTAAAca                             <  1:5066423/71‑2 (MQ=255)

                                       gccaTAGCCATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAgc                                               >  1:2408130/1‑51 (MQ=16)

                                         caTATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCg                          <  1:1392433/71‑1 (MQ=255)

                                           tatGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGAt                                             <  1:1389149/50‑1 (MQ=255)

                                           tatGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTa                                 <  1:2781513/62‑1 (MQ=255)

                                           tatGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGaa                        <  1:1112119/71‑1 (MQ=255)

                                           tatGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGaa                        <  1:4442888/71‑1 (MQ=255)

                                            agccATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTAAACATCaaa                        >  1:2467907/4‑70 (MQ=255)

                                            agccATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTAAACATCaaa                        >  1:4424970/4‑70 (MQ=255)

                                            agccATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTAAACATCaaa                        >  1:4223194/4‑70 (MQ=255)

                                             tGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGa                                              >  1:1136120/1‑47 (MQ=255)

                                             gccATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTaaa                               >  1:35780/3‑62 (MQ=255)

                                             gccATCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTAAACATCaaat                       <  1:4228793/68‑2 (MQ=255)

                                                 tCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATcc                                         <  1:2465098/48‑1 (MQ=16)

                                                 tCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATcc                                         <  1:4288415/48‑1 (MQ=16)

                                                 tCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATcc                                         <  1:3380499/48‑1 (MQ=16)

                                                 tCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCggtg                                     <  1:3678607/52‑3 (MQ=16)

                                                 tCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTa                                 <  1:685436/56‑1 (MQ=255)

                                                 tCTGGTAAACCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTa                                 <  1:145809/56‑1 (MQ=255)

                                                 tCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTa                                 <  1:5121823/56‑1 (MQ=255)

                                                  cTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGccc                 >  1:4247352/1‑71 (MQ=255)

                                                   tGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCa                                                 >  1:2553216/1‑38 (MQ=255)

                                                   tGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTa                                 <  1:1846544/54‑1 (MQ=255)

                                                   tGGCAGACCGGTCAGAACACCAGATTTACGG‑AACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTg               >  1:2475998/1‑71 (MQ=255)

                                                      cAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGaa                        >  1:1376146/1‑60 (MQ=255)

                                                      cAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGaa                        >  1:4702682/1‑60 (MQ=255)

                                                      cAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGaa                        >  1:3868989/1‑60 (MQ=255)

                                                       aaaCCGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTAAACATCAAATACGCCCTGGt             >  1:1024750/3‑70 (MQ=255)

                                                        gACCGGTCAGAACACCAGATTTACGGCAACGCAgg                                               >  1:1265131/1‑35 (MQ=255)

                                                        gACCGGTCAGAACACCAGATTTACGGCAACGCAgg                                               <  1:1134571/35‑1 (MQ=255)

                                                         aCCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTtgt          <  1:183171/71‑1 (MQ=255)

                                                           cGGTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTAAACATCAAATACGCCCTGGTtatgg        >  1:3710304/1‑70 (MQ=255)

                                                            ggTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTa                                 <  1:3069347/45‑1 (MQ=255)

                                                             gTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTAAACATCAAATACGccc                 >  1:4106300/1‑60 (MQ=12)

                                                             gTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTAAACATCAAATACGccc                 >  1:4575174/1‑60 (MQ=12)

                                                             gTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTAAACATCAAATACGccc                 >  1:5571530/1‑60 (MQ=12)

                                                             gTCAGCACGCCAGATTTACGGCAGCGCAGCATATCCGGTGAGTAAACATCAAATACGccc                 >  1:4581481/1‑60 (MQ=12)

                                                             gTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAaca                      >  1:1149188/1‑55 (MQ=255)

                                                              tCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTaaa                               >  1:3094220/1‑45 (MQ=255)

                                                               cAGAACACCAGATTTACGGCAACGCATGATGTCCGGAGTGTAAACGTCg                          <  1:417847/49‑1 (MQ=255)

                                                                aGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGttt    >  1:4540961/1‑70 (MQ=255)

                                                                 gAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTTAACGTCGaa                        >  1:4909086/1‑49 (MQ=255)

                                                                 gAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGaa                        >  1:2810451/1‑49 (MQ=255)

                                                                 gAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGaa                        >  1:1354427/1‑49 (MQ=255)

                                                                  aaCACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAaca                      >  1:3661589/1‑50 (MQ=255)

                                                                  aaCACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTa  >  1:1366394/1‑70 (MQ=255)

CAGCGCAACGCGACGGTAGTCACCGATGATACGGCCACGGCCATATGCATCTGGCAGACCGGTCAGAACACCAGATTTACGGCAACGCAGGATGTCCGGAGTGTAAACGTCGAACACGCCCTGGTTGTGAGTTTTA  >  Exported/609693‑609828

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`