Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 702,818 T→G 7.5% A609A (GCA→GCC) ‡ ymcA ← conserved hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported702,8180TG 7.5% 170.7 / 7.2 67A609A (GCA→GCC) ‡ymcAconserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (33/29);  new base G (2/3);  total (35/32)
Fisher's exact test for biased strand distribution p-value = 6.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.79e-01

ACTGATTGCCGCCCATACCCCTACCGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCTTTTGCCAGATATTGCCCAACGCTAAGTTTCATCAGTACGCCATTGAGCGTCGGCGG  >  Exported/702755‑702886
                                                               |                                                                    
aCTGATTGCCGCCCATACCCCTACCGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGcc                                                                 >  1:4071958/1‑69 (MQ=255)
aCTGATTGCCGCCCATACCCCTACCGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCtt                                                               >  1:1781640/1‑71 (MQ=255)
aCTGATTGCCGCCCATACCCCTACCGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCt                                                                <  1:5111406/70‑1 (MQ=255)
aCTGATTGCCGCCCATACCCCTACCGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCt                                                                <  1:5609738/70‑1 (MQ=255)
 cTGATTGCCGCCCATACCCCTACCGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCtt                                                               >  1:3056408/1‑70 (MQ=255)
    aTTGCCGCCCATACCCCTACCGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTATc                                                           <  1:5502744/71‑1 (MQ=255)
        ccgccCATACCCCTACCGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCttt                                                        <  1:3106262/70‑1 (MQ=255)
          gccCATACCCCTACCGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCTTTTGc                                                     >  1:3342361/1‑71 (MQ=255)
          gccCATACCCCTACCGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCTTTTGc                                                     >  1:1568474/1‑71 (MQ=255)
          gccCATACCCCTACCGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCTTTTGc                                                     >  1:5199373/1‑71 (MQ=255)
             cATACCCCTACCGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCTTTTg                                                      >  1:5674165/1‑67 (MQ=255)
               tACCCCTACCGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTAt                                                            <  1:2749373/59‑1 (MQ=255)
                aCCCCTACCGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGAGCGTTGCGCCTTTATCTTTTGCCAGa                                                 >  1:1238703/1‑69 (MQ=255)
                 ccccTACCGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCtttt                                                       >  1:1812543/1‑62 (MQ=255)
                 ccccTACCGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCTTTTGCCAGatat                                              >  1:2570779/1‑71 (MQ=255)
                 ccccTACCGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCTTTTGCCAGatat                                              >  1:3008816/1‑71 (MQ=255)
                    cTACCGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCTTTTGCCAGata                                               >  1:4318178/1‑67 (MQ=255)
                     tACCGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCtttt                                                       >  1:5010922/1‑58 (MQ=255)
                     tACCGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCtttt                                                       >  1:3411183/1‑58 (MQ=255)
                      accaccACGCCGCTATCAAAGCGTTTGGCGATCTCCAGCGTGCCGCCtt                                                               >  1:891513/1‑49 (MQ=12)
                      accaccACGCCGCTATCAAAGCGTTTGGCGATCTCCAGCGTGCCGCCTTTATCCCCTGCCAGATACTGccc                                         <  1:298773/71‑1 (MQ=255)
                       ccaccACGCCGCTATCAAAGCGTTTGGCGATCTCCAGCGTGCCGCCTTTATCCCCTGCCAGa                                                 <  1:5241443/59‑1 (MQ=255)
                       ccaccACGCCGCTATCAAAGCGTTTGGCGATCTCCAGCGTGCCGCCTTTATCCCCTGCCAGa                                                 <  1:326507/59‑1 (MQ=255)
                       ccaccACGCCGCTATCAAAGCGTTTGGCGATCTCCAGCGTGCCGCCTTTATCCCCTGCCAGATACTGccc                                         >  1:5143422/4‑70 (MQ=255)
                       ccGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCtt                                                               >  1:3179493/1‑48 (MQ=255)
                       ccGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCTTTTGc                                                     >  1:5126942/1‑58 (MQ=255)
                       ccGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCTTTTGCCAGATATTGccc                                         >  1:1884513/1‑70 (MQ=255)
                       ccGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCTTTTGCCAGATATTGccc                                         <  1:881730/70‑1 (MQ=255)
                       ccGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCTTTTGCCAGATATTGccc                                         >  1:3446746/1‑70 (MQ=255)
                       ccGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCTTTTGCCAGATATTGccc                                         >  1:561246/1‑70 (MQ=255)
                        cGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTa                                                             <  1:601751/49‑1 (MQ=255)
                          ccACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCTTTTGCCAGATATTGCCCaa                                       <  1:288315/69‑1 (MQ=255)
                           cACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCTTTTGCCAGATATTGCCCAACGc                                    <  1:90810/71‑1 (MQ=255)
                           cACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCTTTTGCCAGATATTGCCCAACGc                                    <  1:459706/71‑1 (MQ=255)
                           cACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCTTTTGCCAGATATTGCCCAACGc                                    <  1:1653045/71‑1 (MQ=255)
                            aCGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCTTTTGCCAGa                                                 <  1:3561650/57‑1 (MQ=255)
                             cgccgcTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCTTTTGCCAGa                                                 <  1:1531551/56‑1 (MQ=255)
                             cgccgcTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCTTTTGCCAGATATTGCCCAACGCTa                                  <  1:528289/71‑1 (MQ=255)
                              gccgcTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCTTTTGCCAGATATTGCCCAACGCTa                                  <  1:521562/70‑1 (MQ=255)
                                    gTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCTTTTGCCAGATATTGCCCAACGCTAAGTTtca                           >  1:1289911/1‑71 (MQ=255)
                                     tCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCTTTTGCCAGATATTGCCCAACGCTAAGTTtca                           <  1:2857177/70‑1 (MQ=255)
                                       aaaGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCtt                                                         >  1:4887726/1‑38 (MQ=255)
                                       aaaGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCTTTTGGCAGATATTGCCCAACGCTAAGTTtcat                          >  1:418394/1‑69 (MQ=255)
                                       aaaGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCTTTTGCCAGATATTGCCCaa                                       >  1:2892223/1‑56 (MQ=255)
                                        aaGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCTTTTGCCAGATATTGCCCAACGCTAAGTTTCATCAg                       <  1:1924502/71‑1 (MQ=255)
                                         aGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCtt                                                         >  1:2909569/1‑36 (MQ=255)
                                         aGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCtt                                                         >  1:4304310/1‑36 (MQ=255)
                                           cGTTTGGCGACGTCGATCGTTGCGCCTTTATCTTTTGCCAGa                                                 >  1:2043584/1‑42 (MQ=255)
                                           cGTTTGGCGACGTCGATCGTTGCGCCTTTATCTTTTGCAAGa                                                 >  1:1337801/1‑42 (MQ=255)
                                             tttGGCGACGTCGATCGTTGCGCCTTTATCTTTTGcc                                                    <  1:2275777/37‑1 (MQ=255)
                                                   gACGTCGATCGTTGCGCCTTTATCTTTTGCCAGATATTGCCCAACGCTAAGTTTCATCAGTACGCCATTg             <  1:2907826/70‑1 (MQ=255)
                                                     cGTCGATCGTTGCGCCTTTATCTTTTGCCAGATATTGCCCAACGCTAAGTTTCATCAGTACGCCATTGAGc          >  1:2266784/1‑71 (MQ=255)
                                                     cGTCGATCGTTGCGCCTTTATCTTTTGCCAGATATTGCCCAACGCTAAGTTTCATCAGTACGCCATTGAGc          <  1:730130/71‑1 (MQ=255)
                                                     cGTCGATCGTTGCGCCTTTATCTTTTGCCAGATATTGCCCAACGCTAAGTTTCATCAGTACGCCATTGAGc          >  1:1998746/1‑71 (MQ=255)
                                                     cGTCGATCGTTGCGCCTTTATCTTTTGCCAGATATTGCCCAACGCTAAGTTTCATCAGTACGCCATTGAGc          <  1:4467349/71‑1 (MQ=255)
                                                      gTCGATCGTTGCGCCTTTATCTTTTGCCAGATATTGCCCAACGCTAAGTTtca                           >  1:2535448/1‑53 (MQ=255)
                                                       tCGATCGTTGCGCCTTTATCTTTTGCCAGATATTGCCCAACGCTAAGTTtcat                          <  1:4353090/53‑1 (MQ=255)
                                                       tCGATCGTTGCGCCTTTATCTTTTGCCAGATATTGCCCAACGCTAAGTTtca                           <  1:2084100/52‑1 (MQ=255)
                                                           tCGTTGCGCCTTTATCTTTTGCCAGATATTGCCCAACGCTAAGTTTCATCAGTACGCCGTTGAGCGTcggc    >  1:457806/1‑71 (MQ=255)
                                                           tCGTTGCGCCTTTATCTTTTGCCAGATATTGCCCAACGCTAAGTTTCATCAGTACGCCATTGAGCGTcggc    <  1:4804090/71‑1 (MQ=255)
                                                           tCGTTGCGCCTTTATCTTTTGCCAGATATTGCCCAACGCTAAGTTTCATCAGTACGCCATTGAGCGTcggc    >  1:5518192/1‑71 (MQ=255)
                                                           tCGTTGCGCCTTTATCTTTTGCCAGATATTGCCCAACGCTAAGTTTCATCAGTACGCCATTGAGCGTcggc    >  1:5626840/1‑71 (MQ=255)
                                                             gTTGCGCCTTTATCTTTTGCCAGATATTGCCCAACGCTAAGt                               <  1:2109904/42‑1 (MQ=255)
                                                             gTTGCGCCTTTATCTTTTGCCAGATATTGCCCAACGCTAAGt                               <  1:1431816/42‑1 (MQ=255)
                                                             gTTGCGCCTTTATCTTTTGCCAGATATTGCCCAACGCTAAGt                               <  1:675416/42‑1 (MQ=255)
                                                             gTTGCGCCTTTATCTTTTGCCAGATATTGCCCAACGCTAAGTTTCATCAGTACGCCATTGAGCGTcggcgg  <  1:851888/71‑1 (MQ=255)
                                                             gTTGCGCCTTTATCTTTTCCAGATATTGCCCAACGCTAAGt                               <  1:5359143/41‑1 (MQ=255)
                                                              ttGCGCCTTTATCTTTTGCCAGATATTGCCCAACGCTAAg                                <  1:2352886/40‑1 (MQ=255)
                                                               tGCGCCTTTATCTTTTGCCAGATATTGCCCAACGCTAAGttt                             >  1:5560009/1‑42 (MQ=255)
                                                               tGCGCCTTTATCTTTTGCCAGATATTGCCCAACGCTAAGttt                             >  1:2662087/1‑42 (MQ=255)
                                                               tGCGCCTTTATCTTTTGCCAGATATTGCCCAACGCTAAGt                               <  1:1579802/40‑1 (MQ=255)
                                                               tGCGCCTTTATCTTTTGCCAGATATTGCCCAACGCTAAGTTTCATCAGTa                     >  1:3640279/1‑50 (MQ=255)
                                                               |                                                                    
ACTGATTGCCGCCCATACCCCTACCGCCACGCCGCTGTCAAAGCGTTTGGCGACGTCGATCGTTGCGCCTTTATCTTTTGCCAGATATTGCCCAACGCTAAGTTTCATCAGTACGCCATTGAGCGTCGGCGG  >  Exported/702755‑702886

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: