Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 706,680 C→T 68.0% R244K (AGG→AAG)  lpxL ← lauryl‑acyl carrier protein (ACP)‑dependent acyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported706,6800CT68.0% 85.5 / 57.9 75R244K (AGG→AAG) lpxLlauryl‑acyl carrier protein (ACP)‑dependent acyltransferase
Reads supporting (aligned to +/- strand):  ref base C (11/13);  new base T (25/26);  total (36/39)
Fisher's exact test for biased strand distribution p-value = 8.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCGGCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCATCCAGGTTCCGGTCGTGGT  >  Exported/706611‑706750
                                                                     |                                                                      
tCGGCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGcc                                                                        >  1:405395/1‑70 (MQ=255)
tCGGCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGcc                                                                        >  1:4435626/1‑70 (MQ=255)
  ggCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCTTAc                                                                     <  1:3330071/71‑1 (MQ=255)
  ggCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCTTAc                                                                     >  1:683211/1‑71 (MQ=255)
   gCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCTTACg                                                                    <  1:1480346/71‑1 (MQ=255)
     atcatcCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCTTACGGc                                                                  <  1:1499191/71‑1 (MQ=255)
        atcCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCTTACGGCGt                                                                <  1:5252444/70‑1 (MQ=255)
        atcCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCCTACGGCGTg                                                               <  1:5070866/71‑1 (MQ=255)
         tcCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCTTACGGCGTgg                                                              <  1:1929517/71‑1 (MQ=255)
         tcCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCCTACGGCGTg                                                               >  1:4177524/1‑70 (MQ=255)
           cAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCTTAc                                                                     >  1:5202729/1‑62 (MQ=255)
           cAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCTTACGGCGTGGaa                                                            >  1:771878/1‑71 (MQ=255)
             gTGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCATTTGCCATCTGGCTTa                                                                      <  1:230162/59‑1 (MQ=255)
               ggCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCTTACGGCGTGGAACg                                                          >  1:3848430/1‑69 (MQ=255)
                   gagaACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACg                                                          <  1:4786069/65‑1 (MQ=255)
                     gaACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCCTACGGCGt                                                                >  1:3396214/1‑57 (MQ=255)
                      aaCACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGaa                                                        <  1:2719641/64‑1 (MQ=255)
                      aaCACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGaa                                                        <  1:2869639/64‑1 (MQ=255)
                        cacTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCTTACGGCGTGGAACGAAGGGCAc                                                  >  1:2065220/1‑68 (MQ=255)
                        cacTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCTTACGGCGTGGAACGAAGGGCACCAg                                               <  1:2078512/71‑1 (MQ=255)
                            ctGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACAt                                           >  1:3312363/1‑71 (MQ=255)
                            ctGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACAt                                           >  1:1433572/1‑71 (MQ=255)
                             tGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCTTACGGCGTGGAACGAAGGGCACCAGACATg                                          >  1:2262814/1‑71 (MQ=255)
                             tGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCCTACGGCGTgg                                                              <  1:4540582/51‑1 (MQ=255)
                             tGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCCTACGGCGTgg                                                              <  1:3965942/51‑1 (MQ=255)
                             tGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCCTACGGCGTgg                                                              <  1:2592606/51‑1 (MQ=255)
                             tGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATg                                          >  1:859981/1‑71 (MQ=255)
                             tGGCGGCAGCATAATCAATTGATACCCTATGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATg                                          >  1:1003549/1‑71 (MQ=255)
                               gcggcAGCATAATCAATTGATACCCTTTGCCATCTGGCTTACGGCGTGGAACGAAGGGCACCAGACATgcg                                        <  1:870674/71‑1 (MQ=255)
                               gcggcAGCATAATCAATTGATACCCTTTGCCATCTGGCTTACGGCGTGGAACGAAGGGCACCAGACATgc                                         <  1:2143725/70‑1 (MQ=255)
                               gcggcAGCATAATCAATTGATACCCTTTGCCATCTGGCTTACGGCGTGGAACGAAGGGCACCAGACATgc                                         <  1:4120916/70‑1 (MQ=255)
                                 ggcAGCATAATCAATTGATACCCTTTGCCATCTGGCTTACGGCGTGGAACGAAGGGCACCAGACATgcgc                                       <  1:3707976/70‑1 (MQ=255)
                                     gcaTAATCAATTGATACCCTTTGCCATCTGGCTTACGGCGTGGAACGAAggg                                                     <  1:5687728/52‑1 (MQ=255)
                                      caTAATCAATTGATACCCTTTGCCATCTGGCTTACGGCGTGGAACGAAGGGCAc                                                  <  1:5686564/54‑1 (MQ=255)
                                      caTAATCAATTGATACCCTTTGCCATCTGGCTTACGGCGTGGAACGAAGGGCAc                                                  <  1:1332793/54‑1 (MQ=255)
                                       aTAATCAATTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACAt                                >  1:1883669/1‑71 (MQ=255)
                                        tAATCAATTGATACCCTTTGCCATCTGGCTTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATc                               <  1:1732411/71‑1 (MQ=255)
                                        tAATCAATTGATACCCTTTGCCATCTGGCTTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATc                               <  1:498099/71‑1 (MQ=255)
                                        tAATCAATTGATACCCTTTGCCATCTGGCTTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATc                               <  1:1488989/71‑1 (MQ=255)
                                        tAATCAATTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGc                                                    >  1:29518/1‑50 (MQ=255)
                                          aTCAATTGATACCCTTTGCCATCTGGCTTACGGCGTGGAACGAAGGGCACCAGACa                                            >  1:492608/1‑56 (MQ=255)
                                          aTCAATTGATACCCTTTGCCATCTGGCTTACGGCGTGGAACGAAGGGCACCAGACa                                            >  1:5666328/1‑56 (MQ=255)
                                          aTCAATTGATACCCTTTGCCATCTGGCTTACGGCGTGGAACGAAGGGCACCAGACa                                            >  1:2173836/1‑56 (MQ=255)
                                          aTCAATTGATACCCTTTGCCATCTGGCTTACGGCGTGGAACGAAGGGCACCAGACa                                            >  1:1464473/1‑56 (MQ=255)
                                          aTCAATTGATACCCTTTGCCATCTGGCTTACGGCGTGGAACGAAGGGCACCAGACa                                            >  1:252752/1‑56 (MQ=255)
                                          aTCAATTGATACCCTTTGCCATCTGGCTTACGGCGTGGAACGAAGGGCACCAGACa                                            >  1:2489365/1‑56 (MQ=255)
                                          aTCAATTGATACCCTTTGCCATCTGGCTTACGGCGTGGAACGAAGGGCACCAGACa                                            >  1:4166294/1‑56 (MQ=255)
                                          aTCAATTGATACCCTTTGCCATCTGGCTTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCg                             >  1:4814013/1‑71 (MQ=255)
                                          aTCAATTGATACCCTTTGCCATCTGGCTTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCg                             >  1:67273/1‑71 (MQ=255)
                                               ttGATACCCTTTGCCATCTGGCTTACGGCGTGGAACGAAGGGCACCAGACAt                                           <  1:5338626/52‑1 (MQ=255)
                                               ttGATACCCTTTGCCATCTGGCTTACGGCGTGGAACGAAGGGCACCAGACAt                                           <  1:1889321/52‑1 (MQ=255)
                                               ttGATACCCTTTGCCATCTGGCTTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGc                          >  1:1376289/1‑69 (MQ=255)
                                                 gATACCCTTTGCCATCTGGCTTACGGCGTGGAACGa                                                         >  1:1777895/1‑36 (MQ=255)
                                                 gATACCCTTTGCCATCTGGCTTACGGCGTGGAACGAAGGGCACCAGACAt                                           >  1:2483225/1‑50 (MQ=255)
                                                 gATACCCTTTGCCATCTGGCTTACGGCGTGGAACGAAGGGCACCAGACAt                                           >  1:2055499/1‑50 (MQ=255)
                                                  aTACCCTTTGCCATCTGGCTTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGc                      <  1:3215081/70‑1 (MQ=255)
                                                  aTACCCTTTGCCATCTGGCTTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCa                     >  1:421786/1‑71 (MQ=255)
                                                     cccTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCATcc                  <  1:4090322/71‑1 (MQ=255)
                                                     cccTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCATc                   <  1:5008766/70‑1 (MQ=255)
                                                     cccTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCATc                   <  1:2598863/70‑1 (MQ=255)
                                                     cccTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCATc                   <  1:3551589/70‑1 (MQ=255)
                                                      ccTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCATc                   <  1:4203195/69‑1 (MQ=255)
                                                           gCCATCTGGCCTACGGCGTGGAACGAAGGGCACAGACATGCGCCGGACATCCGTGCCAGCATCCAGGTTc            <  1:574531/70‑1 (MQ=255)
                                                              aTCTGGCTTACGGCGTGGAACGAAGGGCACCAGACATGCGCCg                                     <  1:4088636/43‑1 (MQ=255)
                                                              aTCTGGCTTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGa                                   <  1:2283814/45‑1 (MQ=255)
                                                              aTCTGGCTTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGa                                   <  1:3267296/45‑1 (MQ=255)
                                                              aTCTGGCTTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATc                               <  1:3603419/49‑1 (MQ=255)
                                                              aTCTGGCTTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCATCCAGGt              <  1:1354604/66‑1 (MQ=255)
                                                              aTCTGGCTTACGGCGTGGAACGAAGGGCACAAGACATGCGCCGGACATCCGTGCCAGCATCCAGGt              <  1:4751659/66‑1 (MQ=255)
                                                                cTGGCTTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGa                                   >  1:1847570/1‑43 (MQ=255)
                                                                cTGGCTTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGa                                   >  1:4952296/1‑43 (MQ=255)
                                                                cTGGCTTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGa                                   >  1:3954867/1‑43 (MQ=255)
                                                                cTGGCTTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACa                                 >  1:821757/1‑45 (MQ=255)
                                                                 tGGCTTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCATCCAGGTTCCGGTCg      >  1:3304611/1‑71 (MQ=255)
                                                                 tGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCATCCAGGTTCCGGTCg      >  1:5241956/1‑71 (MQ=255)
                                                                 tGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCAAGCATCCAGGTTCCGGTCg      >  1:4517339/1‑71 (MQ=255)
                                                                 tGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGACAGCATCCAGGTTCCGGTCg      >  1:4353489/1‑71 (MQ=255)
                                                                    ccTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCATCCAGGTTCCGGTCGTgg   >  1:397122/1‑71 (MQ=255)
                                                                     cTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCATCCAGGTTCCGGTCGTGGt  <  1:2555206/71‑1 (MQ=255)
                                                                     |                                                                      
TCGGCATCATCCAGTGGCGGAGAACACTCTGGCGGCAGCATAATCAATTGATACCCTTTGCCATCTGGCCTACGGCGTGGAACGAAGGGCACCAGACATGCGCCGGACATCCGTGCCAGCATCCAGGTTCCGGTCGTGGT  >  Exported/706611‑706750

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: