| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 756,325 | G→A | 14.2% | R151H (CGC→CAC) ‡ | lolD → | outer membrane‑specific lipoprotein transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 756,325 | 0 | G | A | 14.2% | 110.0 / 7.3 | 42 | R151H (CGC→CAC) ‡ | lolD | outer membrane‑specific lipoprotein transporter subunit |
| Reads supporting (aligned to +/- strand): ref base G (22/14); new base A (4/2); total (26/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GAGATGTTAAAAGCGGTGGGGCTGGATCATCGTGCGAATCACCGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCGGATGAACCTACCG > Exported/756255‑756393 | gagaTGTTAAAAGCGGTGGGGCTGGATCATCTTGCGAATCACCGCCCATCTGAACTTTCTGGCGGCGAACg > 1:4551754/1‑71 (MQ=255)gagaTGTTAAAAGCGGTGGGGCTGGATCATCGTGCGAATCACCGCCCATCTGAACTTTCTGGCGGCGAACg > 1:891801/1‑71 (MQ=255)gagaTGTTAAAAGCGGTGGGGCTGGATCATCGTGCGAATCACCGCCCATCTGAACTTTCTGGCGGCGAACg > 1:181060/1‑71 (MQ=255)gagaTGTTAAAAGCGGTGGGGCTGGATCATCGTGCGAATCACCGCCCATCTGAACTTTCTGGCGGCGAACg > 1:2222086/1‑71 (MQ=255)gagaTGTTAAAAGCGGTGGGGCTGGATCATCGTGCGAATCACCGCCCATCTGAACTTTCTGGCGGCGAACg > 1:2795022/1‑71 (MQ=255) tGTTAAAAGCGGTGGGGCTGGATCATCGTGCGAATCACCGCCCATCTGAACTTTCTGGCGGCGAACGCCAg < 1:3312325/71‑1 (MQ=255) aaaaGCGGTGGGGCTGGATCATCGTGCGAATCACCGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCgtg > 1:2557694/1‑71 (MQ=255) aaaGCGGTGGGGCTGGATCATCGTGCGAATCACCGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCgtgt > 1:1230763/1‑71 (MQ=255) gCGGTGGGGCTGGATCATCGTGCGAATCACCGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGc < 1:3660592/71‑1 (MQ=255) gCTGGATCATCGTGCGAATCACCGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGa < 1:1465715/65‑1 (MQ=255) gCTGGATCATCGTGCGAATCACCGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGccc > 1:4117877/1‑71 (MQ=255) cTGGATCATCGTGCGAATCACCGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCg > 1:532499/1‑71 (MQ=255) tcatcGTGCGAATCACCGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTgcgc > 1:4637102/1‑71 (MQ=255) acGTGCACATCACTACCCTTCCGAACTTTCTGGTGGTCAACAGCAGCGTGTGGCGATTGCCCGCGCGCTgg < 1:1602388/70‑1 (MQ=9) cGTGCGAATCACCGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCgtg > 1:5556916/1‑49 (MQ=255) cGTGCACATCACTACCCTTCCGAACTTTCTGGTGGTCAACAGCAGCGTGTGGCGATTGCCCGCGCGCTggc > 1:4738873/1‑70 (MQ=9) gAATCACCGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGa > 1:3205053/1‑50 (MQ=255) caTCACTACCCTTCCGAACTTTCTGGTGGTCAACAGCAGCGTGTGGCGATTGccc > 1:3741188/2‑55 (MQ=9) caTCACTACCCTTCCGAACTTTCTGGTGGTCAACAGCAGCGTGTGGCGATTGccc > 1:2360772/2‑55 (MQ=9) aaTCACCGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATaa < 1:295227/71‑1 (MQ=255) aTCACTACCCTTCCGAACTTTCTGGTGGTCAACAGCAGCGTGTGGCGATTGCCCgcg > 1:5039457/1‑55 (MQ=9) aTCACCGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAAc < 1:2119147/71‑1 (MQ=255) tCACCGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAAcc > 1:110642/1‑71 (MQ=255) aCCGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCgt > 1:799255/1‑38 (MQ=255) aCCGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCcg > 1:2491998/1‑71 (MQ=255) ccGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCcgc < 1:2408147/71‑1 (MQ=255) cGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCcgcg > 1:5257174/1‑71 (MQ=255) cGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCcgc > 1:297353/1‑70 (MQ=255) cccATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCTATTGCCCGTCCGCTGGTGAATAACCCGCGcc < 1:747219/71‑1 (MQ=255) cccATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGcc < 1:3835882/71‑1 (MQ=255) tGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGccc > 1:3982004/1‑41 (MQ=255) tGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGt > 1:449435/1‑51 (MQ=255) tGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGt > 1:3204423/1‑51 (MQ=255) tGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGt > 1:4986793/1‑51 (MQ=255) gAACTTTCTGGTGGTCAACAGCAGCGTGTGGCGATTGCCCgcg < 1:2434335/43‑3 (MQ=9) gAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTgg < 1:2890172/67‑1 (MQ=255) aaCTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGt < 1:5209298/67‑1 (MQ=255) aaCTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGt < 1:5636335/67‑1 (MQ=255) aaCTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGt < 1:4163726/67‑1 (MQ=255) aaCTTTCTGGCGGCGAACGCCAGCCTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACTg > 1:1534050/1‑71 (MQ=255) aCTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACTgg > 1:4669592/1‑71 (MQ=255) cTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTAc > 1:1533487/1‑67 (MQ=255) cTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGACCGTGCGCTGGTGAATAACCCGCGCCTGGTAc > 1:430245/1‑67 (MQ=255) tCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCgg < 1:5689915/70‑1 (MQ=255) tCTGGCGGCGAACGCCAGCGTGTGGCGATTGACCGTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCGGa > 1:920414/1‑71 (MQ=255) gcggcgAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCGGATGa < 1:4567061/70‑1 (MQ=255) aCGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCGGATGAACCTACCg > 1:3649286/1‑71 (MQ=255) | GAGATGTTAAAAGCGGTGGGGCTGGATCATCGTGCGAATCACCGCCCATCTGAACTTTCTGGCGGCGAACGCCAGCGTGTGGCGATTGCCCGTGCGCTGGTGAATAACCCGCGCCTGGTACTGGCGGATGAACCTACCG > Exported/756255‑756393 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |