| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 775,487 | C→T | 42.5% | T370T (ACC→ACT) | icd → | isocitrate dehydrogenase, specific for NADP+ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 775,487 | 0 | C | T | 42.5% | 45.1 / 95.8 | 87 | T370T (ACC→ACT) | icd | isocitrate dehydrogenase, specific for NADP+ |
| Reads supporting (aligned to +/- strand): ref base C (20/30); new base T (20/17); total (40/47) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.77e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.71e-01 | |||||||||||
CAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTC > Exported/775419‑775556 | cAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCg > 1:3467118/1‑70 (MQ=255) aGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGaa < 1:3406730/71‑1 (MQ=255) gACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGc < 1:1780961/71‑1 (MQ=255) aaaaGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAg > 1:3128897/2‑68 (MQ=255) aaGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCt > 1:2366848/1‑71 (MQ=255) aTCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCTCCACATGGGTTGGACCGAAGCGGCTGACtt > 1:2604795/1‑70 (MQ=255) gCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTg < 1:2855008/69‑1 (MQ=255) tctATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTa > 1:5074671/1‑70 (MQ=255) ctATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGtt < 1:4630290/68‑1 (MQ=255) tattatTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAg > 1:4560182/1‑71 (MQ=255) attattCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTaa > 1:4121525/1‑61 (MQ=255) ttattCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAAt < 1:4349474/61‑1 (MQ=255) ttattCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAgg < 1:4172312/70‑1 (MQ=255) tattCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGt < 1:1409386/70‑1 (MQ=255) attCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTAt > 1:3348039/1‑71 (MQ=255) ttCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTAt < 1:1629049/70‑1 (MQ=255) tctctcCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATg > 1:991582/1‑70 (MQ=255) tcCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTAt > 1:4111056/1‑65 (MQ=255) tcCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTAt > 1:2455663/1‑65 (MQ=255) ccGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGtt > 1:2983368/1‑56 (MQ=255) cGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAgcc < 1:5646367/70‑3 (MQ=255) cGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGgcg > 1:1289415/1‑71 (MQ=255) cGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGgcg > 1:1284159/1‑71 (MQ=255) cGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGgcg > 1:2200157/1‑71 (MQ=255) gagaTGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAg > 1:1381896/1‑64 (MQ=255) agaTGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATc < 1:1025373/71‑1 (MQ=255) gatgatGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATgg < 1:5351897/59‑1 (MQ=255) gatgatGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATgg < 1:3141336/59‑1 (MQ=255) gatgatGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCa < 1:2100080/71‑1 (MQ=255) gatgatGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCa < 1:327385/71‑1 (MQ=255) ctaatgCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCaa < 1:4250170/68‑1 (MQ=255) gatgCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAAcg > 1:1165095/1‑71 (MQ=255) gatgCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAAcg > 1:94836/1‑71 (MQ=255) aatgCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCaatg > 1:2445968/2‑69 (MQ=255) aatgCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCaatg > 1:3045760/2‑69 (MQ=255) aatgCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCaatg > 1:454705/2‑69 (MQ=255) atgCTGCGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAAtgc > 1:1574736/1‑71 (MQ=255) tgctgcGCCATATGGGTTTGACTTAAGCGGCTGACCTGATTGTTAAAgg < 1:4330108/49‑1 (MQ=255) tgctgcGCCATATGGGTTGGTCTGAAGCGGCTGACCTGATTGTTAAAgg < 1:1080114/49‑1 (MQ=255) tgctgcGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAgg < 1:3782503/49‑1 (MQ=255) gctgcGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTa < 1:5342534/50‑1 (MQ=255) ctgcGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAAtgcca < 1:989573/70‑3 (MQ=255) ctgcGCCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCaa < 1:1203667/71‑1 (MQ=255) ctgcGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGaa < 1:4835775/71‑1 (MQ=255) ctgcGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGa < 1:312725/70‑1 (MQ=255) cgcCATATGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCaag < 1:239143/69‑2 (MQ=255) gcCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAAcc < 1:5537158/71‑1 (MQ=255) ccACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAgg < 1:2080807/42‑1 (MQ=255) ccACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCg < 1:5590726/71‑1 (MQ=255) ataTGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAAtgcc < 1:3276543/60‑2 (MQ=255) ataTGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAAtgcc < 1:1050245/60‑2 (MQ=255) acaTGGGTTGGACCGAAGCGGCTGACTTAATTGTTTAAGGTa < 1:2474800/42‑1 (MQ=255) taTGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAAtgcc < 1:3320052/60‑2 (MQ=255) taTGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAAtgcc < 1:285809/60‑2 (MQ=255) caTGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAgg < 1:1570199/39‑1 (MQ=255) caTGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAgg < 1:320135/39‑1 (MQ=255) caTGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATgg < 1:2398603/44‑1 (MQ=255) caTGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATgg < 1:4666332/44‑1 (MQ=255) caTGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTaa > 1:487027/1‑71 (MQ=255) aTGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGaa > 1:53155/1‑45 (MQ=38) aTGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCaa < 1:3990670/52‑1 (MQ=255) tGGGTTGGACTGAAGCGGCTGACCTGATTGTTaaa > 1:1548558/1‑35 (MQ=25) tGGGTTGGACTGAAGCGGCTGACCTGATTGTTaaa > 1:4521184/1‑35 (MQ=25) tGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTa > 1:2179354/1‑39 (MQ=37) tGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTa > 1:96939/1‑39 (MQ=37) tGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTa > 1:5357055/1‑39 (MQ=37) tGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGgcgc > 1:1761179/1‑49 (MQ=38) tGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCa > 1:1562587/1‑54 (MQ=38) tGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCa > 1:1300947/1‑54 (MQ=38) tGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGAcc > 1:5220178/1‑65 (MQ=255) tGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGAcc > 1:4222670/1‑65 (MQ=255) gggTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAAct > 1:2477880/1‑69 (MQ=255) gggTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAAcc > 1:3917995/1‑70 (MQ=255) ggTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAActt < 1:4593568/70‑3 (MQ=255) ggTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCt < 1:1793318/70‑1 (MQ=255) gTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTAt < 1:1446041/71‑1 (MQ=255) gTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTAt < 1:2038393/71‑1 (MQ=255) ttGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATg < 1:344596/71‑1 (MQ=255) ggACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGa > 1:4061990/1‑37 (MQ=25) ggACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATc > 1:5673379/1‑47 (MQ=37) ggACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCaaga > 1:1993168/1‑55 (MQ=255) ggACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGAc > 1:4050037/1‑71 (MQ=255) gACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACt < 1:561481/71‑1 (MQ=255) gACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACt < 1:3039478/71‑1 (MQ=255) aCCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGgcgc < 1:3058461/41‑1 (MQ=255) aCCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATc < 1:791951/45‑1 (MQ=255) aCCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATc < 1:2646944/45‑1 (MQ=255) ccGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAg > 1:2111704/1‑36 (MQ=255) ccGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTc < 1:1391276/71‑1 (MQ=255) | CAGGACAAAGTAAATCCTGGCTCTATTATTCTCTCCGCTGAGATGATGCTGCGCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTC > Exported/775419‑775556 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |