| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 775,538 | A→G | 39.7% | K387K (AAA→AAG) | icd → | isocitrate dehydrogenase, specific for NADP+ |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 775,538 | 0 | A | G | 39.7% | 94.3 / 128.7 | 121 | K387K (AAA→AAG) | icd | isocitrate dehydrogenase, specific for NADP+ |
| Reads supporting (aligned to +/- strand): ref base A (25/48); new base G (31/17); total (56/65) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.47e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.35e-01 | |||||||||||
Only 100 of 123 total aligned reads displayed.
GCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGAC > Exported/775471‑775607 | gcCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAAcc < 1:5537158/71‑1 (MQ=255) ccACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCg < 1:5590726/71‑1 (MQ=255) caTGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTaa > 1:487027/1‑71 (MQ=255) tGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGAcc > 1:5220178/1‑65 (MQ=255) tGGGTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGAcc > 1:4222670/1‑65 (MQ=255) gggTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAAcc > 1:3917995/1‑70 (MQ=255) ggTTGGACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAActt < 1:4593568/70‑3 (MQ=255) ggTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCt < 1:1793318/70‑1 (MQ=255) gTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTAt < 1:2038393/71‑1 (MQ=255) ggACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGAc > 1:4050037/1‑71 (MQ=255) gACTGAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACt < 1:3039478/71‑1 (MQ=255) ccGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTc < 1:1391276/71‑1 (MQ=255) tgAAGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTc > 1:5559034/2‑70 (MQ=255) aaGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAg > 1:3040542/1‑71 (MQ=255) aaGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAg > 1:5476851/1‑71 (MQ=255) aaGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAg > 1:5344229/1‑71 (MQ=255) aaGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCg < 1:334058/69‑1 (MQ=255) aGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGc < 1:1003337/71‑1 (MQ=255) aGCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGaac > 1:3739297/1‑69 (MQ=255) gCGGCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACg > 1:76223/1‑71 (MQ=255) ggCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGt < 1:4635065/51‑1 (MQ=25) ggCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTc > 1:1230933/1‑71 (MQ=255) gCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCt < 1:3725303/71‑1 (MQ=255) gCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCt < 1:3995916/71‑1 (MQ=255) gCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCt > 1:1036956/1‑71 (MQ=255) gCTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCt < 1:5690598/71‑1 (MQ=255) cTGACCTGATTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGt < 1:5204902/49‑1 (MQ=25) aCTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTgatg > 1:4622093/1‑71 (MQ=255) aCTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTgatg > 1:3232674/1‑71 (MQ=255) ttAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTAt < 1:5277416/51‑1 (MQ=255) ttAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTAt < 1:1634366/51‑1 (MQ=255) ctgaTTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTgatgg > 1:2006119/4‑70 (MQ=255) tAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCg < 1:3817347/57‑1 (MQ=255) gaTTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTgatggaag > 1:2912439/2‑69 (MQ=255) aaTTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTgatggat < 1:5175587/70‑1 (MQ=255) aTTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATg > 1:188082/1‑49 (MQ=25) aTTGTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTgatggaa < 1:4079068/69‑2 (MQ=255) aTTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTAt < 1:1165710/48‑1 (MQ=255) aTTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTgatggatg < 1:311700/70‑1 (MQ=255) ttGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGatggatgg < 1:4256673/70‑1 (MQ=255) tGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTgatgga > 1:3630303/1‑66 (MQ=255) gTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAAc > 1:2432984/1‑41 (MQ=25) gTTAAAGGTATGGAAGGCGCAATCAATGCCAAGACCGTAAc > 1:5672166/1‑41 (MQ=25) gTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGgcgc > 1:349504/1‑71 (MQ=255) aaaGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTa < 1:3914945/70‑1 (MQ=255) aaaGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTa < 1:536297/70‑1 (MQ=255) aaGGTATGGAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTaag > 1:5649732/1‑70 (MQ=255) aaGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTaaa < 1:2884133/71‑1 (MQ=255) ggTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGGCTGATGGATGGCGCTAAAct < 1:3408985/71‑1 (MQ=255) gTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTc < 1:3137538/51‑1 (MQ=255) gTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAActg > 1:210280/1‑71 (MQ=255) tATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGa < 1:868828/45‑1 (MQ=255) tATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGa < 1:2077164/45‑1 (MQ=255) tATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGa < 1:2508219/45‑1 (MQ=255) tGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGa < 1:2561306/43‑1 (MQ=255) tGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTgatg < 1:1580396/53‑1 (MQ=255) gAAGGCGCAATCAATGCCAAGACCGTAACTTATGa > 1:1542456/1‑35 (MQ=12) gAAGGCGCAATCAATGCCAAGACCGTAACTTATGa > 1:4330831/1‑35 (MQ=12) gAAGGCGCAATCAATGCCAAGACCGTAACTTATGa > 1:1970754/1‑35 (MQ=12) gAAGGCGCAATCAATGCCAAGACCGTAACTTATGa > 1:3726986/1‑35 (MQ=12) gAAGGCGCAATCAATGCCAAGACCGTAACTTATGa > 1:5521586/1‑35 (MQ=12) gAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTc > 1:791745/1‑46 (MQ=21) gAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTc > 1:1877086/1‑46 (MQ=21) gAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTc > 1:4726941/1‑46 (MQ=21) gAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTga > 1:2877551/1‑49 (MQ=21) gAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTga > 1:1332639/1‑49 (MQ=21) gAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTga > 1:1332533/1‑49 (MQ=21) gAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGaa < 1:3614765/71‑1 (MQ=255) gAAGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGa > 1:1651041/1‑70 (MQ=255) gAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCg < 1:2690109/40‑1 (MQ=255) gAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCg < 1:1430011/40‑1 (MQ=255) gAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTgatgga > 1:1150515/1‑53 (MQ=255) gAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTgatgga > 1:1494705/1‑53 (MQ=255) gAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGa > 1:1523257/1‑70 (MQ=255) gAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGa > 1:2422665/1‑70 (MQ=255) gAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGa > 1:2611725/1‑70 (MQ=255) gAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGa > 1:3780148/1‑70 (MQ=255) gAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGCATGGCGCTAAACTGCTGaa < 1:1418655/71‑1 (MQ=255) aaGGCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGaaa > 1:3417508/1‑71 (MQ=255) aaGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGaaa > 1:4507738/1‑71 (MQ=255) ggCGCAATCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGGCGCTAAGCTGCTGaaa > 1:4129865/1‑69 (MQ=255) gcgcAATCAATGCCAAGACCGTAACTTATGACTTCGAACGt < 1:1197820/41‑1 (MQ=12) cgcAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGtt < 1:379986/71‑1 (MQ=255) cAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTaaa > 1:2774159/1‑56 (MQ=255) cAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGtt > 1:2122522/1‑69 (MQ=255) aTCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGatggatgg > 1:1237810/1‑47 (MQ=255) tCAATGCCAAGACCGTAACTTATGACTTCGAACGTCTGATGGAAGgcg < 1:3514670/48‑1 (MQ=16) tCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGtt < 1:2951441/66‑1 (MQ=255) tCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCagag > 1:437349/1‑71 (MQ=255) tCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCagag > 1:1999120/1‑71 (MQ=255) aaCGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTa < 1:3365003/49‑1 (MQ=255) aaCGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAgtgt < 1:1036445/70‑1 (MQ=255) aCGGGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTaaa < 1:112212/50‑1 (MQ=255) aCGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTaaa < 1:1823507/50‑1 (MQ=255) aCGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGttt < 1:2189580/71‑1 (MQ=255) cgAAAACCGTAACCTATGACTTCGAGCGTCTGatggatgg > 1:577322/1‑40 (MQ=255) cgAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGt < 1:2182948/71‑1 (MQ=255) gAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGt > 1:758211/1‑70 (MQ=255) aaaCCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGAc > 1:4910777/1‑71 (MQ=255) aaCCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGaaa > 1:3692746/1‑52 (MQ=255) | GCCACATGGGTTGGACCGAAGCGGCTGACTTAATTGTTAAAGGTATGGAAGGCGCAATCAACGCGAAAACCGTAACCTATGACTTCGAGCGTCTGATGGATGGCGCTAAACTGCTGAAATGTTCAGAGTTTGGTGAC > Exported/775471‑775607 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |