Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 821,744 C→T 39.9% intergenic (‑281/+173) ldrA ← / ← ldrC toxic polypeptide, small/toxic polypeptide, small

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported821,7440CT39.9% 90.4 / 93.8 93intergenic (‑281/+173)ldrA/ldrCtoxic polypeptide, small/toxic polypeptide, small
Reads supporting (aligned to +/- strand):  ref base C (25/30);  new base T (18/19);  total (44/49)
Fisher's exact test for biased strand distribution p-value = 8.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTT  >  Exported/821679‑821814
                                                                 |                                                                      
gATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCtttt                                                                    >  1:1104757/1‑70 (MQ=40)
gATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTc                                                                   <  1:5258918/71‑1 (MQ=39)
gATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTc                                                                   <  1:5623243/71‑1 (MQ=39)
  tactacGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCaa                                                                 >  1:3703521/1‑71 (MQ=39)
  tactacGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCaa                                                                 >  1:3998554/1‑71 (MQ=39)
   actacGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCaa                                                                 >  1:4528678/1‑70 (MQ=40)
    ctacGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAAc                                                                <  1:1392233/70‑1 (MQ=40)
             aaTTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTg                                                        <  1:2788131/69‑1 (MQ=35)
                 cATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCtttt                                                                    >  1:2354029/1‑53 (MQ=35)
                 cATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCtttt                                                                    >  1:4463368/1‑53 (MQ=35)
                 cATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACa                                                               >  1:3531416/1‑58 (MQ=35)
                 cATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGc                                                             >  1:636252/1‑60 (MQ=35)
                  aTCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGt                                                 <  1:3024025/71‑1 (MQ=39)
                     ttcacttcaTCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGtt                                                >  1:3937129/1‑69 (MQ=40)
                     ttcacttcaTCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGt                                                 <  1:4887246/68‑1 (MQ=40)
                     ttcacttcaTCCCTGAAATGTTTGCAATAAAGAGAACATTCCGGCTTTTCAACAGCTGTTGCAGTCGtt                                                >  1:320400/1‑69 (MQ=255)
                      tcacttcaTCCCTGAAATGTTTGCAATAAAGAGAACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTc                                              <  1:576134/70‑1 (MQ=255)
                       cacttcaTCCCTGAAATGTTTGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCa                                             >  1:12353/1‑70 (MQ=11)
                       cacttcaTCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGttt                                               <  1:1446410/68‑1 (MQ=40)
                       cacttcaTCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTc                                              <  1:4797940/69‑1 (MQ=40)
                       cacttcaTCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTc                                              <  1:2007512/69‑1 (MQ=40)
                       cacttcaTCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTc                                              <  1:766275/69‑1 (MQ=40)
                       cacttcaTCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCa                                             >  1:2046808/1‑70 (MQ=255)
                       cacttcaTCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCAt                                            >  1:2184858/1‑71 (MQ=255)
                        acttcaTCCCTGAAATGTTTGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCAt                                            <  1:5603295/70‑1 (MQ=11)
                        acttcaTCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCAt                                            <  1:523176/70‑1 (MQ=255)
                        acttcaTCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCAt                                            <  1:2873495/70‑1 (MQ=255)
                        acttcaTCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCAt                                            <  1:4844769/70‑1 (MQ=255)
                        acttcaTCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATg                                           <  1:216736/71‑1 (MQ=255)
                        acttcaTCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATg                                           <  1:2383303/71‑1 (MQ=255)
                         cttcaTCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTc                                              <  1:5234717/67‑1 (MQ=40)
                         cttcaTCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGa                                          <  1:1444423/71‑1 (MQ=255)
                            caTCCCTGAAATGTTTGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGa                                          >  1:1055425/1‑68 (MQ=11)
                            caTCCCTGAAATGTTTGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTg                                       >  1:3842299/1‑71 (MQ=14)
                             aTCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGc                                      <  1:1585178/71‑1 (MQ=255)
                              tCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCAt                                            <  1:4156454/64‑1 (MQ=255)
                               cccTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGct                                     >  1:5006544/1‑70 (MQ=255)
                                ccTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGc                                                             >  1:5061367/1‑45 (MQ=38)
                                ccTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGc                                                             >  1:83291/1‑45 (MQ=38)
                                 cTGAAATGTTTGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTg                                  <  1:4718052/71‑1 (MQ=17)
                                  tGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTc                                                                   <  1:1754790/37‑1 (MQ=40)
                                   gAAATGTTTGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGga                                >  1:2975512/1‑71 (MQ=17)
                                   gAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGctc                                    >  1:2800009/1‑67 (MQ=255)
                                   gAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGctc                                    >  1:3716784/1‑67 (MQ=255)
                                    aaaTGTTTGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCAt                                            <  1:1047272/58‑1 (MQ=11)
                                    aaaTGTTTGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGgat                               <  1:976120/71‑1 (MQ=17)
                                    aaaTGTTTGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGgat                               <  1:2869499/71‑1 (MQ=17)
                                    aaaTGTTTGCAATAAAGAGTACATTCCGGCTTTTCaa                                                                 >  1:5271687/1‑37 (MQ=40)
                                    aaaTGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTgg                                 <  1:4647999/69‑1 (MQ=255)
                                    aaaTGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGgat                               <  1:3052047/71‑1 (MQ=255)
                                    aaaTGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGgat                               <  1:3996150/71‑1 (MQ=255)
                                    aaaTGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGgat                               <  1:5206207/71‑1 (MQ=255)
                                    aaaTGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGga                                >  1:807401/1‑70 (MQ=255)
                                     aaTGTTTGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGgatg                              <  1:3749695/71‑1 (MQ=18)
                                         tttGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGc                          >  1:385316/1‑71 (MQ=18)
                                          ttGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGgatg                              >  1:4706826/1‑66 (MQ=18)
                                          ttGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCt                         >  1:1955198/1‑71 (MQ=18)
                                          ttGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGc                                                       >  1:2712986/1‑41 (MQ=38)
                                           tGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGgatgag                            >  1:5250076/1‑66 (MQ=18)
                                           tGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGgatgag                            >  1:4322892/1‑66 (MQ=18)
                                           tGCAATGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCt                         <  1:2690654/70‑1 (MQ=18)
                                           tGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTg                                       >  1:4844364/1‑56 (MQ=255)
                                              aaTGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTg                                  <  1:1423380/58‑1 (MQ=17)
                                              aaTGAAGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTg                                  <  1:4827439/58‑1 (MQ=17)
                                               aTAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGc                                                       <  1:4906049/36‑1 (MQ=40)
                                                tgaaGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATg                                           <  1:4325493/45‑1 (MQ=14)
                                                tgaaGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATg                                           <  1:1688084/45‑1 (MQ=14)
                                                 aaaGAGTACATTCCGGCTTTTCAACAGCTGTTGCAg                                                     >  1:2203093/1‑36 (MQ=33)
                                                  aaGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGgatga                             >  1:504803/1‑59 (MQ=21)
                                                  aaGAGTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTc                 <  1:5614866/71‑1 (MQ=25)
                                                    gagTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCgg               <  1:329877/71‑1 (MQ=37)
                                                    gagTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCgg               <  1:3616340/71‑1 (MQ=37)
                                                    gagTGCATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCgg               >  1:3947259/1‑71 (MQ=37)
                                                    gagTGCATTCCGGTTTTTAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCggg              >  1:1143634/1‑71 (MQ=255)
                                                     agTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCa                                             <  1:4530933/40‑1 (MQ=255)
                                                     agTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGgatga                             <  1:2635013/56‑1 (MQ=255)
                                                      gtgcATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGt             <  1:3826935/68‑1 (MQ=37)
                                                       tgcATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGgatga                             >  1:2663971/3‑54 (MQ=25)
                                                       tACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGt                                        >  1:1192865/1‑43 (MQ=255)
                                                       tACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGatgatg                           >  1:4154224/1‑56 (MQ=255)
                                                        aCATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTg           >  1:4311177/1‑71 (MQ=255)
                                                         cATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCAt                                            >  1:5547527/1‑37 (MQ=17)
                                                         cATTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGa                                          >  1:1312919/1‑39 (MQ=17)
                                                          aTTCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGcc         <  1:501107/71‑1 (MQ=38)
                                                           ttCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAg                    <  1:545143/59‑1 (MQ=37)
                                                           ttCCGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAg                    <  1:1082392/59‑1 (MQ=37)
                                                             ccGGTTTTTCAACAGCTGTTACAGTCATTTCATGa                                          >  1:650708/1‑35 (MQ=18)
                                                             ccGGTTTTTCAACAGCTGTTACAGTCATTTCATGa                                          >  1:2092500/1‑35 (MQ=18)
                                                             ccGGTTTTTCAACAGCTGTTACAGTCATTTCATGa                                          >  1:226352/1‑35 (MQ=18)
                                                             ccGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGGATGAGGCTTCCAGCTCGGGTTGCCaa       <  1:2800549/70‑1 (MQ=255)
                                                             ccGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCaa       <  1:5418685/70‑1 (MQ=255)
                                                              cGGTTTTTCAACAGCTGTTACAGTCATTTCATGAGTGCTCTGga                                >  1:1791373/1‑44 (MQ=25)
                                                              cGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCgg               <  1:2158097/61‑1 (MQ=255)
                                                                 cTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGc                          >  1:2022357/1‑47 (MQ=255)
                                                                 cTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGc                          >  1:1070892/1‑47 (MQ=255)
                                                                 cTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATAttt  <  1:4230168/71‑1 (MQ=255)
                                                                 cTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATAttt  <  1:5015339/71‑1 (MQ=255)
                                                                 |                                                                      
GATACTACGTCTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATAAAGAGTACATTCCGGCTTTTCAACAGCTGTTGCAGTCGTTTCATGAGTGCTCTGGATGATGCTTCCAGCTCGGGTTGCCAATATTT  >  Exported/821679‑821814

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: