Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 189,567 C→A 18.1% A355E (GCG→GAG) ‡ ldcC → lysine decarboxylase 2, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported189,5670CA18.1% 173.0 / 19.3 66A355E (GCG→GAG) ‡ldcClysine decarboxylase 2, constitutive
Reads supporting (aligned to +/- strand):  ref base C (25/29);  new base A (5/7);  total (30/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CATTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGAT  >  Exported/189515‑189636
                                                    |                                                                     
cATTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGa                                                     <  1:208090/71‑1 (MQ=255)
 aTTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGa                                                     >  1:4125926/1‑70 (MQ=255)
 aTTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGa                                                     >  1:4794873/1‑70 (MQ=255)
  ttttCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCg                                                      <  1:4439234/68‑1 (MQ=255)
   tttCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGaaat                                                  >  1:1807745/1‑70 (MQ=255)
    ttCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACg                                                 >  1:5517853/1‑71 (MQ=255)
      cATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGc                                                <  1:4558411/70‑1 (MQ=255)
       aTCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCaa                                              >  1:3819833/1‑71 (MQ=255)
       aTCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCaa                                              >  1:1999617/1‑71 (MQ=255)
       aTCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCaa                                              >  1:3298468/1‑71 (MQ=255)
        tCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAAt                                             <  1:1594876/71‑1 (MQ=255)
        accGATTTACGAAGGTAAATGCGGTATGAGCGGTGGCCGTGTAGAAGGGAAAGTGATTTACGAAAccc                                                <  1:33132/67‑3 (MQ=255)
         ccGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATc                                            >  1:4838050/1‑71 (MQ=255)
         ccGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATc                                            >  1:1319715/1‑71 (MQ=255)
          cGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATc                                            >  1:3192380/1‑70 (MQ=255)
           gATTTACGAAGGTAAATGCGGTATGAGCGGTGGCCGTGTAGAAGGGAAAGTGAt                                                           <  1:4494528/54‑1 (MQ=255)
           gATTTACGAAGGTAAATGCGGTATGAGCGGTGGCCGTGTAGAAGGGAAAGTGAt                                                           <  1:5542532/54‑1 (MQ=255)
           gATTTACGAAGGTAAATGCGGTATGAGCGGTGGCCGTGTAGAAGGGAAAGTGATTTACGAAACCCAGTcca                                          <  1:2364591/71‑3 (MQ=255)
           gATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGa                                          >  1:3906376/1‑71 (MQ=255)
             acTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGAcc                                        >  1:5670592/2‑71 (MQ=255)
                aCCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCca                                      >  1:161151/1‑70 (MQ=255)
                 ccAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATc                                            <  1:1944296/63‑1 (MQ=255)
                 ccAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGa                                          >  1:1068464/1‑65 (MQ=255)
                 ccAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGa                                          >  1:5037802/1‑65 (MQ=255)
                 ccAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCcaca                                    <  1:4386824/71‑1 (MQ=255)
                 ccAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCcac                                     >  1:3364452/1‑70 (MQ=255)
                 ccAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCcac                                     >  1:365569/1‑70 (MQ=255)
                  gaaggTAAATGCGGTATGAGCGGTGTCCGTGTAGAAGGGAAAGTGATTTACGAAACCCAGTCCACTCACaa                                   <  1:2288477/68‑1 (MQ=255)
                  cAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGa                                                     <  1:2804454/53‑1 (MQ=255)
                  cAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGa                                                     <  1:2448136/53‑1 (MQ=255)
                  cAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGa                                                     <  1:3126184/53‑1 (MQ=255)
                  cAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGa                                                     <  1:731261/53‑1 (MQ=255)
                  cAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGa                                                     <  1:360930/53‑1 (MQ=255)
                  cAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACaa                                   <  1:1677336/71‑1 (MQ=255)
                   aaggTAAATGCGGTATGAGCGGTGGCCGTGTAGAAGGGAAAGTGATTTACGAAACCCAGTCCACTCACaaa                                  >  1:3386987/3‑71 (MQ=255)
                     ggTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAAt                                <  1:4932725/71‑1 (MQ=255)
                       tAAATGCGGTATGAGCGGTGGCCGTGTAGAAGGGAAAGTGATTTACGAAACCCAGTCCACTCACAAACTGc                              <  1:4873280/71‑1 (MQ=255)
                       tAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGc                                                <  1:1186021/53‑1 (MQ=255)
                       tAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGc                                                <  1:4177264/53‑1 (MQ=255)
                       tAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGc                                                <  1:4325413/53‑1 (MQ=255)
                       tAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGc                                                <  1:4392755/53‑1 (MQ=255)
                        aaaaGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCt                             >  1:1720803/1‑71 (MQ=255)
                         aaaGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCtt                                                        >  1:250502/1‑43 (MQ=255)
                          aaGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTgg                           <  1:1277332/71‑1 (MQ=255)
                          aaGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTgg                           >  1:4387942/1‑71 (MQ=255)
                             tGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACaaaa                                 >  1:1664180/1‑62 (MQ=255)
                             cggTATGAGCGGTGGCCGTGTAGAAGGGAAAGTGATTTACGAAACCCAGTCCACTCACaa                                   >  1:4637517/2‑60 (MQ=255)
                             cggTATGAGCGGTGGCCGTGTAGAAGGGAAAGTGATTTACGAAACACAGTCCACTCACaa                                   >  1:808938/2‑60 (MQ=255)
                               gTATGAGCGGTGGCCGTGTAGAAGGGAAAGTGATTTACGAAACCCAGTCCACTCACAAACTGCTGgcggcg                      >  1:765353/1‑71 (MQ=255)
                                 aTGAGCGGTGGCCGTGTAGAAGGGAAAGTGATTTACGAAACCCAGTCCACTCACAAACTGCTGGCGGCGtt                    >  1:3310451/1‑71 (MQ=255)
                                  tGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGa                                                     <  1:3555802/37‑1 (MQ=255)
                                  tGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGa                                                     <  1:1044632/37‑1 (MQ=255)
                                  tGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGa                                                     <  1:5656078/37‑1 (MQ=255)
                                  tGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGtt                    <  1:4972451/70‑1 (MQ=255)
                                  tGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTa                   <  1:4594300/71‑1 (MQ=255)
                                   gAGCGGTGGCCGTGTAGAAGGGAAAGTGATTTACGAAACCCAGTCCACTCACAAACTGCTGGCGGCGtt                    <  1:3147560/69‑1 (MQ=255)
                                    aGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGaa                                                    >  1:2471900/1‑36 (MQ=255)
                                    aGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGaa                                                    >  1:2804721/1‑36 (MQ=255)
                                     gcggcgAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCg                <  1:5024137/71‑1 (MQ=255)
                                        gcgAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAg             >  1:5478784/1‑71 (MQ=255)
                                        gcgAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAg             >  1:1361244/1‑71 (MQ=255)
                                               tgtTGCGGGAAAAGAGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCa              <  1:4531633/63‑1 (MQ=255)
                                                 ttGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAAt                                <  1:193018/43‑1 (MQ=255)
                                                 ttGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATTCTGGCGGCGTTATCGCAGGCTTCGCt     <  1:1891243/70‑1 (MQ=255)
                                                 ttGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCt     <  1:4195592/70‑1 (MQ=255)
                                                 ttGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCt     <  1:1688458/70‑1 (MQ=255)
                                                    cGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGAt  <  1:1403813/70‑1 (MQ=255)
                                                    |                                                                     
CATTTTCATCCGATCTACCAGGGTAAAAGTGGTATGAGCGGCGAGCGTGTTGCGGGAAAAGTGATCTTCGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGAT  >  Exported/189515‑189636

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: