| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 822,222 | T→A | 18.2% | intergenic (‑198/+206) | ldrC ← / ← chaA | toxic polypeptide, small/calcium/sodium:proton antiporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 822,222 | 0 | T | A | 18.2% | 291.1 / 54.1 | 121 | intergenic (‑198/+206) | ldrC/chaA | toxic polypeptide, small/calcium/sodium:proton antiporter |
| Reads supporting (aligned to +/- strand): ref base T (42/57); new base A (11/11); total (53/68) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.36e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01 | |||||||||||
Only 100 of 123 total aligned reads displayed.
TGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAA > Exported/822153‑822285 | tGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTc > 1:122295/1‑71 (MQ=255)tGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTc < 1:4953620/71‑1 (MQ=255) accaGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCaa < 1:3772732/71‑1 (MQ=255) accaGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCaa < 1:599951/71‑1 (MQ=255) ccaGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGTATACTACGTCGCAAt < 1:3480473/71‑1 (MQ=255) ccaGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAAt > 1:1379073/1‑71 (MQ=255) aGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTc < 1:4451800/71‑1 (MQ=255) ggATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCa > 1:319644/1‑70 (MQ=255) gATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATc < 1:886652/71‑1 (MQ=255) gATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATc < 1:1662705/71‑1 (MQ=255) aaGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATcttca > 1:5087104/1‑71 (MQ=255) ccccGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATcttcactt < 1:83780/71‑1 (MQ=255) cGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGACTCAATTCATCTTCACTTCAt > 1:1714408/1‑71 (MQ=255) gCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTc > 1:1394750/1‑57 (MQ=255) gCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCa < 1:1350270/58‑1 (MQ=255) gCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCa < 1:5676508/58‑1 (MQ=255) gCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATc < 1:5680689/60‑1 (MQ=255) gCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGACTCAATTCATCTTCACTTCATc > 1:4467792/1‑71 (MQ=255) cAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAAt < 1:2390254/54‑1 (MQ=255) cATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTg < 1:1168377/71‑1 (MQ=255) aTTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTg > 1:395404/1‑70 (MQ=255) aTTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGACTCAATTCATCTTCACTTCATCCCTGa < 1:252645/71‑1 (MQ=255) ttGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGa > 1:3643404/1‑70 (MQ=255) gcgcCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGACTCAATTCATCTTCACTTCATCCCTGaaa < 1:4268317/70‑1 (MQ=255) gcgcCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGACTCAATTCATCTTCACTTCATCCCTGAAAt > 1:2072270/1‑71 (MQ=255) cgcCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTc < 1:5627899/48‑1 (MQ=255) cgcCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAAt < 1:2088267/70‑1 (MQ=255) cgcCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAAt < 1:5077101/70‑1 (MQ=255) cTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCAt > 1:4101863/1‑58 (MQ=255) cTCACCGGATAACGCCGGCTTGGTGTGGATACTACGACTCAATTCATCTTCACTTCATCCCTGAAATGttt < 1:4209075/71‑1 (MQ=255) tCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGc < 1:3699999/39‑1 (MQ=255) tCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGc < 1:4055554/39‑1 (MQ=255) tCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGaaa < 1:5540844/65‑1 (MQ=255) tCACCGGATAACGCCGGCTTGGTGTGGATACTACGACTCAATTCATCTTCACTTCATCCCTGAAATGTTTg > 1:2711550/1‑71 (MQ=255) tCACCGGATAACGCCGGCTTGGTGTGGATACTACGACTCAATTCATCTTCACTTCATCCCTGAAATGTTTg > 1:3794510/1‑71 (MQ=255) cACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGaaa < 1:5119410/64‑1 (MQ=255) aCCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGc < 1:178178/70‑1 (MQ=255) aCCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGc > 1:4915399/1‑70 (MQ=255) aCCGGATAACGCCGGCTTGGTGTGGATACTACGACTCAATTCATcttc > 1:2872262/1‑48 (MQ=11) aCCGGATAACGCCGGCTTGGTGTGGATACTACGACTCAATTCATcttc > 1:86823/1‑48 (MQ=11) aCCGGATAACGCCGGCTTGGTGTGGATACTACGACTCAATTCATCTTCACTTCATCCCTGAAATGt < 1:2789590/66‑1 (MQ=255) aCCGGATAACGCCGGCTTGGTGTGGATACTACGACTCAATTCATCTTCACTTCATCCCTGAAATGt < 1:2058814/66‑1 (MQ=255) ccGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCaa > 1:3472988/1‑71 (MQ=255) cGGATAACGCCGGCTTGGTGTGGATACTACGTCGCaa > 1:3772991/1‑37 (MQ=255) cGGATAACGCCGGCTTGGTGTGGATACTACGTCGCaa > 1:3074835/1‑37 (MQ=255) cGGATAACGCCGGCTTGGTGTGGATACTACGACTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAAt < 1:3962130/71‑1 (MQ=255) cGGATAACGCCGGCTTGGTGTGGATACTACGACTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAAt < 1:5287372/71‑1 (MQ=255) ggATAACGCCGGCTTGGTGTGGATACTACGACTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAAt > 1:5511009/1‑70 (MQ=255) gATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATcttca > 1:4295043/1‑45 (MQ=255) gATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATcttca > 1:3820851/1‑45 (MQ=255) aTAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGaa < 1:4759336/71‑1 (MQ=255) tAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATcttc < 1:4876622/42‑1 (MQ=255) tAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGttt < 1:3406352/62‑1 (MQ=255) tAACGCCGGCTTGGTGTGGATACTACGACTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAag < 1:1151289/71‑1 (MQ=255) tAACGCCGGCTTGGTGTGGATACTACGACTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAag < 1:2377871/71‑1 (MQ=255) tAACGCCGGCTTGGTGTGGATAC‑ACGACTCAATTCATCTTCACTTCATCCCTGAAATGTTTGCaa > 1:1681164/1‑65 (MQ=11) aaCGCCGGCTTGGTGTGGATACTACGTCGCAATTCATcttcactt > 1:2747774/1‑45 (MQ=255) aaCGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTAATCCCTGAAATg > 1:3432142/1‑58 (MQ=255) aCGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAagag < 1:1366693/71‑1 (MQ=255) aCGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTCAAATGTTTGc > 1:2331591/1‑62 (MQ=255) ccGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTa > 1:4998389/1‑70 (MQ=40) cGGCTTGGTGTGGATACTACGTCGCAATTCATcttca > 1:4099330/1‑37 (MQ=40) cGGCTTGGTGTGGATACTACGTCGCAATTCATcttca > 1:4686396/1‑37 (MQ=40) ggCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCAGAAATGTTTGCAATGAaga > 1:2568887/1‑65 (MQ=255) cTTGGTGTGGATACTACGTCGCAATTCATCTGCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTc < 1:295500/71‑1 (MQ=255) tGGTGTGGATACTACGTCGCAATTCATcttcacttca > 1:436697/1‑37 (MQ=40) tGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCg > 1:4329109/1‑71 (MQ=39) tGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCg > 1:4335476/1‑71 (MQ=39) tGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCg > 1:755966/1‑71 (MQ=39) gtgtGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCgg < 1:2021548/70‑1 (MQ=40) gtgGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAag < 1:4311928/56‑1 (MQ=255) gtgGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAag < 1:103331/56‑1 (MQ=255) gtgGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAag < 1:1964152/56‑1 (MQ=255) tgGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCt < 1:4948895/69‑1 (MQ=40) gATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCtttt < 1:1141021/70‑1 (MQ=40) gATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTc < 1:2363604/71‑1 (MQ=39) gATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTc < 1:3260012/71‑1 (MQ=39) tactacGTCGCAATTCATCTTCACTTCATCCCTGa < 1:1658343/35‑1 (MQ=40) tactacGTCGCAATTCATCTTCACTTCATCCCTGa < 1:2615220/35‑1 (MQ=40) tactacGTCGCAATTCATCTTCACTTCATCCCTGa < 1:5367366/35‑1 (MQ=40) tactacGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCaa < 1:2968349/71‑1 (MQ=39) actacGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACa > 1:360394/1‑56 (MQ=255) actacGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACa > 1:2521769/1‑56 (MQ=255) actacGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACa > 1:2261381/1‑56 (MQ=255) ctacGTCGCAATTCATCTTCACTTCATCCCTGAAATg < 1:1968366/37‑1 (MQ=40) ctacGTCGCAATTCATCTTCACTTCATCCCTGAAATg < 1:4764979/37‑1 (MQ=40) ctacGTCGCAATTCATCTTCACTTCATCCCTGAAATg < 1:4758551/37‑1 (MQ=40) tacGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAag < 1:4779499/48‑1 (MQ=255) tacGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAag < 1:871361/48‑1 (MQ=255) tacGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAag < 1:3611319/48‑1 (MQ=255) tacGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCaa > 1:2006096/1‑68 (MQ=40) acGTCGCAATTCATCTTCACTTCATCCCTGAAATGtt > 1:2047014/1‑37 (MQ=40) acGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCaa > 1:4560989/1‑42 (MQ=38) cGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAagag > 1:530545/1‑48 (MQ=255) cGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAagag > 1:3934197/1‑48 (MQ=255) gTCGCAATTCATCTTCACTTCATCCCTGCAATGtt < 1:731112/35‑1 (MQ=255) gTCGCAATTCATCTTCACTTCATCCCTGAAATGtt < 1:374791/35‑1 (MQ=40) gTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGc < 1:4869741/38‑1 (MQ=40) tCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGc < 1:5482184/37‑1 (MQ=40) tCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGc < 1:639143/37‑1 (MQ=40) | TGCCACCAGGGATAAAGCCCCGCAACATTGCGCCTCACCGGATAACGCCGGCTTGGTGTGGATACTACGTCGCAATTCATCTTCACTTCATCCCTGAAATGTTTGCAATGAAGAGTACATTCCGGCTTTTCAA > Exported/822153‑822285 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |