| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 832,070 | C→G | 10.9% | Y176* (TAC→TAG) ‡ | narG → | nitrate reductase 1, alpha subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 832,070 | 0 | C | G | 10.9% | 171.8 / 10.6 | 64 | Y176* (TAC→TAG) ‡ | narG | nitrate reductase 1, alpha subunit |
| Reads supporting (aligned to +/- strand): ref base C (30/27); new base G (3/4); total (33/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.04e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01 | |||||||||||
GACGCGGTGGATTTGTTCGTTCTTCCTGGCAGGAGGTGAACGAACTGATCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTT > Exported/832003‑832140 | gACGCGGTGGATTTGTTCGTTCATCCTGGCAGGAGGTGAACGAACTGATCGCCGCATCTAACGTTTacac > 1:651955/1‑70 (MQ=255) cgttcTTCCTGGCAGGAGGTGAACGAACTGATCGCCGCATCTAACGTTTac > 1:5235618/1‑51 (MQ=255) cgttcTTCCTGGCAGGAGGTGAACGAACTGATCGCCGCATCTAACGTTTac > 1:2212690/1‑51 (MQ=255) cgttcTTCCTGGCAGGAGGTGAACGAACTGATCGCCGCATCTAACGTTTACACCATCaaaaa > 1:2992601/1‑62 (MQ=255) cgttcTTCCTGGCAGGAGGTGAACGAACTGATCGCCGCATCTAACGTTTACACCATCAAAAAc > 1:2268023/1‑63 (MQ=255) ttcttcCTGGCAGGAGGTGAACGAACTGATCGCCGCATCTAACGTTTACACCATCaaa < 1:340700/58‑1 (MQ=255) cttcCTGGCAGGAGGTGAACGAACTGATCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCCCGGAc > 1:349094/1‑71 (MQ=255) ccTGGCAGGAGGTGAACGAACTGATCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCCCGGAc < 1:2145428/68‑1 (MQ=255) cTGGCAGGAGGTGAACGAACTGATCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCCCGGACCg < 1:4029823/69‑1 (MQ=255) aggTGAACGAACTGATCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCCCGGACCGTGTTGCTGGt < 1:215467/71‑1 (MQ=255) gaacgaacTGATCGCCGCATCTAACGTTTACACCATCAAAAACTAc > 1:352943/1‑46 (MQ=255) gaacgaacTGATCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTtct > 1:493275/1‑71 (MQ=255) gaacgaacTGATCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTtct > 1:4393584/1‑71 (MQ=255) gaacgaacTGATCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTtct > 1:4941474/1‑71 (MQ=255) aacgaacTGATCGCCGCATCTAACGTTTACACCATCAAAAATTACGGCCCGGACCGTGTCGCTGGCTtctc > 1:2628453/1‑71 (MQ=255) aacgaacTGATCGCCGCATCTAACGTTTACACCATCAAAAATTACGGCCCGGACCGTGTCGCTGGCTtctc > 1:4040059/1‑71 (MQ=255) aacgaacTGATCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTtct < 1:131151/70‑1 (MQ=255) aacgaacTGATCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTtc < 1:5481344/69‑1 (MQ=255) cgaacTGATCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTTCTCGc > 1:3485223/1‑71 (MQ=255) gaacTGATCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTTCTCGcc < 1:4976961/71‑1 (MQ=255) cTGATCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAAt < 1:4964170/71‑1 (MQ=255) gATCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCCCGGAc > 1:1732775/1‑46 (MQ=255) attgccgccGCTAACGTCTGGACCATCAAAACCTACGGCCCGGATCGCGTTGCCGGTTTCTCGCCGATccc < 1:3152349/68‑1 (MQ=255) aTCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCCCGGACCgtgt < 1:4243162/50‑1 (MQ=255) aTCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTcc > 1:936587/1‑71 (MQ=255) tCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCCCGGACCgtg < 1:4168738/48‑1 (MQ=255) tCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAAt > 1:1866958/1‑67 (MQ=255) tCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTCCg > 1:2305049/1‑71 (MQ=255) cgccgcATCTAACGTTTACACCATCAAAAACTACGGCCCGGACCGTGTTg > 1:3150246/1‑50 (MQ=255) gccgccGCTAACGTCTGGACCATCAAAACCTACGGCCCGGATCGCGTTGCCGGTTTCTCGCCGATCCCGGc > 1:4053174/1‑71 (MQ=255) gccgccGCTAACGTCTGGACCATCAAAACCTACGGCCCGGATCGCGTTGCCGGTTTCTCGCCGATCCCGGc > 1:634764/1‑71 (MQ=255) gccgcATCTAACGTTTACACCATCAAAAACTACGGCCCGGACCGTGTCGCTGGTTTCTCGCCAATTCCGGc < 1:2273519/71‑1 (MQ=255) cgcATCTAACGTTTACACCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCaa > 1:123709/1‑71 (MQ=255) gcATCTAACGTTTACACCATCAAAAACTACGGCCCGGACCgtg < 1:3474836/43‑1 (MQ=255) cATCTAACGTTTACACCATCAAAAACTACGGCCCGGACCGTGTTGCTGGtt > 1:2335341/1‑51 (MQ=255) cATCTAACGTTTACACCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATg < 1:5015116/71‑1 (MQ=255) tCTAACGTTTACACCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTc < 1:4360545/61‑1 (MQ=255) tCTAACGTTTACACCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGc < 1:993397/65‑1 (MQ=255) tCTAACGTTTACACCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGc < 1:2610061/65‑1 (MQ=255) tCTAACGTTTACACCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTc > 1:4559810/1‑71 (MQ=255) gcTAACGTCTGGACCATCAAAACCTACGGCCCGGATCGCGTTGCCGGTTTCTCGCCGATCCCGGc < 1:183876/64‑1 (MQ=255) gcTAACGTCTGGACCATCAAAACCTACGGCCCGGATCGCGTTGCCGGTTTCTCGCCGATCCCGGCGATGTc < 1:5170591/70‑1 (MQ=255) tAACGTTTACACCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTcc > 1:5432170/1‑60 (MQ=255) tAACGTTTACACCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGc < 1:2670855/63‑1 (MQ=255) tAACGTCTGGACCATCAAAACCTACGGCCCGGATCGCGTTGCCGGtt < 1:2404767/47‑1 (MQ=255) tAACGTCTGGACCATCAAAACCTACGGCCCGGATCGCGTTGCCGGTTtct > 1:5250444/1‑50 (MQ=255) aaCGTTTACACCATCAAAAACTACGGCCCGGACCgtgt > 1:1158981/1‑38 (MQ=255) aaCGTTTACACCATCAAAAACTACGGCCCGGACCgtgt > 1:1888533/1‑38 (MQ=255) aaCGTTTACACCATCAAAAACTACGGCCCGGACCgtg > 1:1733049/1‑37 (MQ=255) aaCGTTTACACCATCAAAAACTACGGCCCGGACCgtg > 1:3044162/1‑37 (MQ=255) aaCGTTTACACCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCg > 1:517407/1‑69 (MQ=255) aaCGTTTACACCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGAt > 1:317158/1‑71 (MQ=255) aCGTTTACACCATCAAAAACTACGGCCCGGACCgtgt < 1:2798108/37‑1 (MQ=255) aCGTTTACACCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTCCg < 1:3344827/59‑1 (MQ=255) aCGTTTACACCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCaa > 1:2961017/1‑63 (MQ=255) aCGTTTACACCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTc < 1:3704031/67‑1 (MQ=255) aCGTTTACACCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTc < 1:2022767/67‑1 (MQ=255) cGTTTACACCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTtctc > 1:2013047/1‑48 (MQ=255) gTTTACACCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGt > 1:1435766/1‑71 (MQ=255) tttACACCATCAAAAACTACGGCCCGGACCGTGTTGc < 1:1025657/37‑1 (MQ=255) tttACACCATCAAAAACTACGGCCCGGACCGTGTTGCt < 1:3938579/38‑1 (MQ=255) tttACACCATCAAAAACTACGGCCCGGACCGTGTTGCt < 1:3931721/38‑1 (MQ=255) tttACACCATCAAAAACTACGGCCCGGACCGTGTTGCt < 1:5180480/38‑1 (MQ=255) ttACACCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGtt > 1:3071241/1‑70 (MQ=255) tACACCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATg > 1:3673520/1‑60 (MQ=255) tACACCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGttt < 1:3578637/70‑1 (MQ=255) tACACCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGttt < 1:2123143/70‑1 (MQ=255) cacCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCtt < 1:1033628/71‑1 (MQ=255) cacCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCt < 1:3327177/70‑1 (MQ=255) | GACGCGGTGGATTTGTTCGTTCTTCCTGGCAGGAGGTGAACGAACTGATCGCCGCATCTAACGTTTACACCATCAAAAACTACGGCCCGGACCGTGTTGCTGGTTTCTCGCCAATTCCGGCAATGTCGATGGTTTCTT > Exported/832003‑832140 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |